Abstract:
BACKGROUND:The Rapid-i is a new FDA cleared closed carrier for embryo vitrification. The cooling rate of - 1220°C/min is far lower than that reported with open vitrification systems such as the cryoloop (-15,000°C/min). Little published data is currently available on this device. This study presents our initial clinical data, as well as live birth outcomes, with the Rapid-i. The efficacy of this device for the cryopreservation of cleavage, as well as blastocyst stage human embryos is also analyzed. We further compare outcomes to those achieved with the cryoloop, an "open" vitrification system routinely used in our laboratory. METHODS:Human embryos were vitrified at either the 8-10 cell stage or else the blastocyst stage. The vitrification protocol was: 7.5% DMSO/7.5% ethylene glycol (EG) (2-3 min) followed by incubation in 15% DMSO /15% EG (45 sec) before loading on the vitrification carrier. Cryoprotectant was removed during warming by sequential washes in 0.25 M and 0.125 M sucrose in culture medium. Clinical outcome data for frozen cycles between January 2011 and August 2012 were stratified according to carrier and cell stage. The student t-test and chi square test were used to compare results. P value of < 0.05 was considered significant. RESULTS:A total of 486 vitrified-warmed embryos were assessed and 92% of them were transferred. The clinical pregnancy rate (CPR) and implantation rate (IR) with Rapid-i vitrified blastocysts were 59% and 49%, versus 47% and 37%, respectively for cleavage stage embryos. This was not statistically different from results with the cryoloop vitrified blastocysts (CPR 46%, IR 38%) nor the cleavage stage vitrified embryos (CPR 49%, IR 35%). To date, there have been 31 deliveries of 34 healthy infants from Rapid-i vitrified embryos, with another 12 pregnancies still on-going. CONCLUSIONS:The Rapid-i offers an excellent alternative to existing open vitrification devices for embryo cryopreservation at the 8-10 cell stage as well as the blastocyst stage. Use of this type of "closed" sealed system that prevents direct contact between the embryos and liquid nitrogen reduces the potential risk of sample cross-contamination or infection. These preliminary data and live birth outcomes have paved the way toward transitioning to a closed vitrification system in our own IVF program.
journal_name
Reprod Biol Endocrinoljournal_title
Reproductive biology and endocrinology : RB&Eauthors
Desai NN,Goldberg JM,Austin C,Falcone Tdoi
10.1186/1477-7827-11-41subject
Has Abstractpub_date
2013-05-15 00:00:00pages
41issn
1477-7827pii
1477-7827-11-41journal_volume
11pub_type
杂志文章abstract:BACKGROUND:There is limited literature investigating the effects of body mass index (BMI) and androgen level on in vitro fertilization (IVF) outcomes with a gonadotropin-releasing hormone (GnRH)-antagonist protocol in polycystic ovary syndrome (PCOS). Androgen-related variation in the effect of body mass index (BMI) on...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/s12958-018-0438-7
更新日期:2018-11-19 00:00:00
abstract:BACKGROUND:In assisted reproductive technology, prediction of treatment failure remains a great challenge. The development of more sensitive assays for measuring anti-Müllerian hormone (AMH) has allowed for the possibility to investigate if a lower threshold of AMH can be established predicting very limited or no respo...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/s12958-019-0452-4
更新日期:2019-01-11 00:00:00
abstract:BACKGROUND:During the estrous cycle, the rat uterine endometrium undergoes many changes such as cell proliferation and apoptosis. If implantation occurs, stromal cells differentiate into decidual cells and near the end of pregnancy, a second wave of apoptosis occurs. This process called decidual regression, is tightly ...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-7-80
更新日期:2009-08-05 00:00:00
abstract::The response of Meishan conceptuses to an exogenous precursor for oestradiol-17beta biosynthesis was investigated in vitro, to determine whether gestational age or morphological stage of development elicit changes in hormone metabolism. Conceptuses were recovered on days 11, 12, 13 or 15 after the onset of oestrus and...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-1-16
更新日期:2003-02-11 00:00:00
abstract:BACKGROUND:Several kinds of anti-oxidants have drawn a lot of intention for their benefits on vascular protection. In addition, it has been demonstrated that exercise training could improve endothelial function by up-regulating endothelial nitric oxide synthase (eNOS) protein. Therefore, the present study aims to inves...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-7-93
更新日期:2009-09-07 00:00:00
abstract:BACKGROUND:Despite being born with a significant number of primordial cells which representing the ancestor cells of the germ-line, women experience a depletion of ovarian reserve and sub-fertility mid-way into their healthy lives. The poor ovarian response is a substantial limiting factor amplified with higher materna...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/s12958-019-0464-0
更新日期:2019-02-11 00:00:00
abstract:BACKGROUND:In early pregnancy, increased plasma levels of the endocannabinoid anandamide (AEA) are associated with miscarriage through mechanisms that might affect the developing placenta or maternal decidua. METHODS:In this study, we compare AEA levels in failed and viable pregnancies with the levels of the trophobla...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-9-152
更新日期:2011-11-29 00:00:00
abstract::The endometrium undergoes cyclic remodelling throughout the menstrual cycle in preparation for embryo implantation which occurs in a short window during the mid-secretory phase. It is during this short 'receptive window' that the endometrial luminal epithelium acquires adhesive capacity permitting blastocysts firm adh...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/s12958-020-00682-0
更新日期:2020-12-14 00:00:00
abstract:BACKGROUND:Meta-analysis revealed that embryo placement 20 mm from the fundal endometrial surface resulted in higher pregnancy rate, ongoing pregnancy rate, and live birth rate compared with placement 10 mm from the fundal endometrial surface. Pregnancy and implantation rates according to relative position were higher ...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章,随机对照试验
doi:10.1186/s12958-015-0072-6
更新日期:2015-07-29 00:00:00
abstract:BACKGROUND:To evaluate the association between leukocytes (polymorphonuclear granulocytes -PMNL) and semen parameters at different leukocyte concentrations. METHODS:This was a retrospective clinical study at a university hospital andrology clinic. Semen samples from infertile men were analyzed for sperm morphology and...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-8-12
更新日期:2010-02-05 00:00:00
abstract:BACKGROUND:Endometriosis is a known cause of infertility. Differences in immune tolerance caused by regulatory T cells (Tregs) and transforming growth factor-β (TGF-β) are thought to be involved in the pathology of endometriosis. Evidence has indicated that Tregs can be separated into three functionally and phenotypica...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/s12958-018-0325-2
更新日期:2018-02-01 00:00:00
abstract:BACKGROUND:To examine the effects of IVF, ICSI and FET, as well as in vitro culture, on the safety of offspring, this study was conducted from the perspective of genetic imprinting to investigate whether assisted reproductive technology would influence the parental and maternal imprinting genes. METHODS:Eighteen foetu...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/s12958-018-0344-z
更新日期:2018-07-05 00:00:00
abstract:BACKGROUND:Leukocytes represent the predominant source of reactive oxygen species both in seminal plasma and in sperm suspensions and have been demonstrated to negatively influence sperm function and fertilization rate in assisted reproduction procedures. Peroxidase test is the standard method recommended by WHO to det...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-7-128
更新日期:2009-11-19 00:00:00
abstract:OBJECTIVES:To report the occurrence of a rare case of a huge benign ovarian tumour (mucinous cystadenoma) in Jazan city, Saudi Arabia. PATIENTS:Our reported case was a middle-aged Saudi woman presented with marked abdominal distension and discomfort at the gynaecology clinic of Jazan General Hospital, Jazan city, Saud...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-8-24
更新日期:2010-03-11 00:00:00
abstract:BACKGROUND:Considering that clinical trial studies are limited in polycystic ovary syndrome (PCOS) patients, and there is no consensus on an optimum endometrial preparation protocol for frozen embryo transfer (FET), the present study was designed as a randomized clinical trial to compare the reproductive outcomes follo...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/s12958-020-00675-z
更新日期:2020-11-20 00:00:00
abstract:BACKGROUND:The E2F family of transcription factors is required for the activation or repression of differentially expressed gene programs during the cell cycle in normal and abnormal development of tissues. We previously determined that members of the retinoblastoma protein family that interacts with the E2F family are...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-4-63
更新日期:2006-12-05 00:00:00
abstract::Human embryonic stem cells (hESC) have emerged as attractive candidates for cell-based therapies that are capable of restoring lost cell and tissue function. These unique cells are able to self-renew indefinitely and have the capacity to differentiate in to all three germ layers (ectoderm, endoderm and mesoderm). Harn...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 历史文章,杂志文章,评审
doi:10.1186/s12958-015-0005-4
更新日期:2015-02-22 00:00:00
abstract:BACKGROUND:With infertility populations rapidly aging, treatments improving pregnancy chances assume increasing clinical importance. Dehydroepiandrosterone (DHEA) has been reported to improve pregnancy rates and lower miscarriage rates in women with diminished ovarian function. This study was planned to evaluate whethe...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章,随机对照试验
doi:10.1186/s12958-015-0014-3
更新日期:2015-03-09 00:00:00
abstract:BACKGROUND:Brain-derived ectonucleoside triphosphate diphosphohydrolases (NTPDases) have been known as plasma membrane-incorporated enzymes with their ATP-hydrolyzing domain outside of the cell. As such, these enzymes are thought to regulate purinergic intercellular signaling by hydrolyzing ATP to ADP-AMP, thus regulat...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章,评审
doi:10.1186/1477-7827-7-63
更新日期:2009-06-16 00:00:00
abstract:BACKGROUND:Previous caesarean scar pregnancy is one type of ectopic pregnancy in myometrium and fibrous tissue of previous caesarean scar. One of the therapeutic methods of this type of ectopic pregnancy is treatment with methotrexate. Given various findings on the treatment of caesarean scar pregnancy with methotrexat...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/s12958-020-00666-0
更新日期:2020-11-09 00:00:00
abstract:BACKGROUND:L-carnitine (LC), and its acetylated form, acetyl L-carnitine (ALC), have immense functional capabilities to regulate the oxidative and metabolic status of the female reproductive system. The vulnerability of this system to free radicals demand for advanced strategies to combat them. For this purpose, the 'q...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章,评审
doi:10.1186/s12958-018-0323-4
更新日期:2018-01-26 00:00:00
abstract::During the follicular phase of the primate menstrual cycle, a single follicle usually matures to the preovulatory stage and releases its oocyte for fertilization and the potential establishment of pregnancy. In assisted reproductive technology procedures, it is desirable to override the natural process of follicle sel...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章,评审
doi:10.1186/1477-7827-2-31
更新日期:2004-06-16 00:00:00
abstract:BACKGROUND:Relaxin is detected in seminal plasma of many species and its association with sperm motility may be beneficial in some aspects of assisted reproduction. Here, we immunolocalized relaxin receptors and investigated the effects of exogenous relaxin on motility characteristics, viability, and cAMP content of bo...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/s12958-015-0021-4
更新日期:2015-03-31 00:00:00
abstract::Gonadotropin-primed immature rats (GPIR) constitute a widely used model for the study of ovulation. Although the equivalence between the ovulatory process in immature and adult rats is generally assumed, the morphological and functional characteristics of ovulation in immature rats have been scarcely considered. We de...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-2-63
更新日期:2004-09-03 00:00:00
abstract:BACKGROUND:High levels of dopamine (DA) were described in human ovary and recently evidence for DA receptors in granulosa and luteal cells has been provided, as well. However, neither the full repertoire of ovarian receptors for DA, nor their specific role, is established. Human granulosa cells (GCs) derived from women...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-5-40
更新日期:2007-10-25 00:00:00
abstract::For the extrapolation of data obtained from experimental animals to the human situation, it is important to know the similarities and differences between human and animal species. Some important characteristics of nitric oxide synthase (NOS) in myometrium and vagina from human and rat were compared. NOS-activity was m...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-1-10
更新日期:2003-02-06 00:00:00
abstract:BACKGROUND:Doxorubicin is a potent chemotherapeutic drug used against a variety of cancers. It acts through interaction with polymerases and topoisomerase II and free radical production. Doxorubicin activity is not specific to cancer cells and can also damage healthy cells, especially those undergoing rapid proliferati...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-10-79
更新日期:2012-09-11 00:00:00
abstract:BACKGROUND:A decrease in HOXA11 expression in eutopic mid-secretory endometrium has been found in women with endometriosis-associated infertility. METHODS:Using Real-time quantitative PCR (RQ-PCR) and western blotting analysis we studied the HOXA11 transcript and protein levels in mid-luteal eutopic endometrium from e...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-10-1
更新日期:2012-01-10 00:00:00
abstract:BACKGROUND:To study the expression of Plexin-B1, Glycodelin, and MMP7 during the menstrual cycle in the endometrium and in the fallopian tube. METHODS:The research included women undergoing hysterectomy, tubal sterilization or salpingo-oophoerectomy. Total RNA from endometrial and fallopian tube tissues was extracted ...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-7-152
更新日期:2009-12-29 00:00:00
abstract:BACKGROUND:Infertile men have higher levels of semen reactive oxygen species (ROS) than fertile men. High levels of semen ROS can cause sperm dysfunction, sperm DNA damage and reduced male reproductive potential. This study investigated the effects of supplementation with N-acetyl-cysteine (NAC) on the sperm quality, c...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章,随机对照试验
doi:10.1186/s12958-019-0468-9
更新日期:2019-02-16 00:00:00