Transgelin interacts with PARP1 in human colon cancer cells.

Abstract:

Background:Transgelin, an actin-binding protein, is associated with cytoskeleton remodeling. Findings from our previous studies demonstrated that transgelin was up-regulated in node-positive colorectal cancer (CRC) versus node-negative disease. Over-expression of TAGLN affected the expression of 256 downstream transcripts and increased the metastatic potential of colon cancer cells in vitro and in vivo. This study aims to explore the mechanisms through which transgelin participates in the metastasis of colon cancer cells. Methods:Immunofluorescence and immunoblotting analysis were used to determine the cellular localization of endogenous and exogenous transgelin in colon cancer cells. Co-immunoprecipitation and subsequently high-performance liquid chromatography/tandem mass spectrometry were performed to identify the proteins that were potentially interacting with transgelin. The 256 downstream transcripts regulated by transgelin were analyzed with bioinformatics methods to discriminate the specific key genes and signaling pathways. The Gene-Cloud of Biotechnology Information (GCBI) tools were used to predict the potential transcription factors (TFs) for the key genes. The predicted TFs corresponded to the proteins identified to interact with transgelin. The interaction between transgelin and the TFs was verified by co-immunoprecipitation and immunofluorescence. Results:Transgelin was found to localize in both the cytoplasm and nucleus of the colon cancer cells. Approximately 297 proteins were identified to interact with transgelin. The overexpression of TAGLN led to the differential expression of 184 downstream genes. Network topology analysis discriminated seven key genes, including CALM1, MYO1F, NCKIPSD, PLK4, RAC1, WAS and WIPF1, which are mostly involved in the Rho signaling pathway. Poly (ADP-ribose) polymerase-1 (PARP1) was predicted as the unique TF for the key genes and concurrently corresponded to the DNA-binding proteins potentially interacting with transgelin. The interaction between PARP1 and transgelin in human RKO colon cancer cells was further validated by immunoprecipitation and immunofluorescence assays. Conclusions:Our results suggest that transgelin binds to PARP1 and regulates the expression of downstream key genes, which are mainly involved in the Rho signaling pathway, and thus participates in the metastasis of colon cancer.

journal_name

Cancer Cell Int

authors

Lew ZX,Zhou HM,Fang YY,Ye Z,Zhong W,Yang XY,Yu Z,Chen DY,Luo SM,Chen LF,Lin Y

doi

10.1186/s12935-020-01461-y

subject

Has Abstract

pub_date

2020-08-03 00:00:00

pages

366

issn

1475-2867

pii

1461

journal_volume

20

pub_type

杂志文章
  • NEAT1/miR-23a-3p/KLF3: a novel regulatory axis in melanoma cancer progression.

    abstract:Background:Melanoma is an extremely aggressive malignant skin tumor with high mortality. Many types of long noncoding RNAs and microRNAs have been reported to be associated with the oncogenesis of melanoma. However, a novel lncRNA-NEAT has not been thoroughly investigated in melanoma cancer. The purposes of this study ...

    journal_title:Cancer cell international

    pub_type: 杂志文章

    doi:10.1186/s12935-019-0927-6

    authors: Ding F,Lai J,Gao Y,Wang G,Shang J,Zhang D,Zheng S

    更新日期:2019-08-22 00:00:00

  • Long noncoding RNA DLX6-AS1 promotes neuroblastoma progression by regulating miR-107/BDNF pathway.

    abstract:Background:Long noncoding RNAs (lncRNAs) play essential roles in tumor progression. However, the functions and targets of lncRNAs in neuroblastoma (NB) progression still remain to be determined. In this study, we aimed to investigate the effect of lncRNA DLX6 antisense RNA 1 (DLX6-AS1) on NB and the underlying mechanis...

    journal_title:Cancer cell international

    pub_type: 杂志文章

    doi:10.1186/s12935-019-0968-x

    authors: Zhang HY,Xing MQ,Guo J,Zhao JC,Chen X,Jiang Z,Zhang H,Dong Q

    更新日期:2019-11-27 00:00:00

  • Survivin: a unique target for tumor therapy.

    abstract::Survivin is the smallest member of the Inhibitor of apoptosis (IAP) family of proteins, involved in inhibition of apoptosis and regulation of cell cycle. These functional attributes make Survivin a unique protein exhibiting divergent functions i.e. regulating cell proliferation and cell death. Expression pattern of Su...

    journal_title:Cancer cell international

    pub_type: 杂志文章,评审

    doi:10.1186/s12935-016-0326-1

    authors: Garg H,Suri P,Gupta JC,Talwar GP,Dubey S

    更新日期:2016-06-23 00:00:00

  • Verteporfin disrupts multiple steps of autophagy and regulates p53 to sensitize osteosarcoma cells.

    abstract:BACKGROUND:Osteosarcoma (OS) is a malignant tumor of the bone mostly observed in children and adolescents. The current treatment approach includes neoadjuvant and adjuvant chemotherapy; however, drug resistance often hinders therapy in OS patients. Also, the post-relapse survival of OS patients is as low as 20%. We the...

    journal_title:Cancer cell international

    pub_type: 杂志文章

    doi:10.1186/s12935-020-01720-y

    authors: Saini H,Sharma H,Mukherjee S,Chowdhury S,Chowdhury R

    更新日期:2021-01-14 00:00:00

  • Dual EGFR and BRAF blockade overcomes resistance to vemurafenib in BRAF mutated thyroid carcinoma cells.

    abstract:BACKGROUND:BRAF inhibitors are effective anticancer agents in BRAF-mutated melanomas. By contrast, evidences about sensitivity of thyroid carcinomas to BRAF inhibition are conflicting and it has been proposed that BRAF V600E thyroid carcinoma cells are less sensitive to BRAF inhibitors due to activation of parallel sig...

    journal_title:Cancer cell international

    pub_type: 杂志文章

    doi:10.1186/s12935-017-0457-z

    authors: Notarangelo T,Sisinni L,Condelli V,Landriscina M

    更新日期:2017-10-04 00:00:00

  • Caspase-2 is involved in cell death induction by taxanes in breast cancer cells.

    abstract:BACKGROUND:We studied the role of caspase-2 in apoptosis induction by taxanes (paclitaxel, novel taxane SB-T-1216) in breast cancer cells using SK-BR-3 (nonfunctional p53, functional caspase-3) and MCF-7 (functional p53, nonfunctional caspase-3) cell lines. RESULTS:Both taxanes induced apoptosis in SK-BR-3 as well as ...

    journal_title:Cancer cell international

    pub_type: 杂志文章

    doi:10.1186/1475-2867-13-42

    authors: Jelínek M,Balušíková K,Kopperová D,Nĕmcová-Fürstová V,Šrámek J,Fidlerová J,Zanardi I,Ojima I,Kovář J

    更新日期:2013-05-15 00:00:00

  • BTG1 inhibits malignancy as a novel prognosis signature in endometrial carcinoma.

    abstract:Background:Endometrial carcinoma (EC) is one of the three major malignant tumors of the female reproductive system. In recent years, the incidence and mortality rate of EC have increased. B-cell translocation gene 1 (BTG1) is an anti-proliferation gene that regulates the occurrence and development of a variety of tumor...

    journal_title:Cancer cell international

    pub_type: 杂志文章

    doi:10.1186/s12935-020-01591-3

    authors: Li Y,Huo J,He J,Zhang Y,Ma X

    更新日期:2020-10-07 00:00:00

  • The histone demethylase JMJD2A promotes glioma cell growth via targeting Akt-mTOR signaling.

    abstract:Background:A number of JmjC domain-containing histone demethylases have been identified and biochemically characterized in mammalian models and humans. JMJD2A is a transcriptional co-factor and enzyme that catalyzes the demethylation of histone H3 lysine 9 and 36 (H3K9 and H3K36). Here in this study, we reported the ro...

    journal_title:Cancer cell international

    pub_type: 杂志文章

    doi:10.1186/s12935-020-01177-z

    authors: Li M,Cheng J,Ma Y,Guo H,Shu H,Huang H,Kuang Y,Yang T

    更新日期:2020-03-30 00:00:00

  • Changes in P-glycoprotein activity are mediated by the growth of a tumour cell line as multicellular spheroids.

    abstract:BACKGROUND:Expression of P-glycoprotein (P-gp), the multidrug resistance (MDR) 1 gene product, can lead to multidrug resistance in tumours. However, the physiological role of P-gp in tumours growing as multicellular spheroids is not well understood. Recent evidence suggests that P-gp activity may be modulated by cellul...

    journal_title:Cancer cell international

    pub_type: 杂志文章

    doi:10.1186/1475-2867-5-20

    authors: Ponce de León V,Barrera-Rodríguez R

    更新日期:2005-07-07 00:00:00

  • Overexpression of microRNA-29b inhibits epithelial-mesenchymal transition and angiogenesis of colorectal cancer through the ETV4/ERK/EGFR axis.

    abstract:BACKGROUND:Recent studies have reported the involvement of microRNA-29 (miR-29) family members in human cancers through their ability to regulate cellular functions. The present study investigated biological function of miR-29b in colorectal cancer (CRC). METHODS:CRC tissues and adjacent normal tissues were collected ...

    journal_title:Cancer cell international

    pub_type: 杂志文章

    doi:10.1186/s12935-020-01700-2

    authors: Leng Y,Chen Z,Ding H,Zhao X,Qin L,Pan Y

    更新日期:2021-01-06 00:00:00

  • CDK5RAP3 inhibits angiogenesis in gastric neuroendocrine carcinoma by modulating AKT/HIF-1α/VEGFA signaling.

    abstract:Background:Angiogenesis plays critical roles in the progression and metastasis of malignant tumors. Gastric neuroendocrine carcinoma is an uncommon stomach cancer that is rich in blood vessels and exhibits highly malignant biological behavior with a poor prognosis. The role of CDK5RAP3 in GNEC has not been reported to ...

    journal_title:Cancer cell international

    pub_type: 杂志文章

    doi:10.1186/s12935-019-0997-5

    authors: Lin JX,Weng XF,Xie XS,Lian NZ,Qiu SL,Wang JB,Lu J,Chen QY,Cao LL,Lin M,Tu RH,Yang YH,Liu SJ,Hu M,Lin YK,Huang CM,Zheng CH,Li P,Xie JW

    更新日期:2019-11-07 00:00:00

  • CircNEIL3 promotes cervical cancer cell proliferation by adsorbing miR-137 and upregulating KLF12.

    abstract:BACKGROUND:CircRNAs play crucial roles in multiple tumours. However, the functions of most circRNAs in cervical cancer remain unclear. METHODS:This study collected GSE113696 data from the GEO database to search for differentially expressed circRNAs in cervical cancer. Quantitative reverse transcription PCR was used to...

    journal_title:Cancer cell international

    pub_type: 杂志文章

    doi:10.1186/s12935-020-01736-4

    authors: Chen Y,Geng Y,Huang J,Xi D,Xu G,Gu W,Shao Y

    更新日期:2021-01-07 00:00:00

  • Understanding the colon cancer stem cells and perspectives on treatment.

    abstract::An area of research that has been recently gaining attention is the relationship between cancer stem cell (CSC) biology and chemo-resistance in colon cancer patients. It is well recognized that tumor initiation, growth, invasion and metastasis are promoted by CSCs. An important reason for the widespread interest in th...

    journal_title:Cancer cell international

    pub_type: 杂志文章

    doi:10.1186/s12935-015-0163-7

    authors: Garza-Treviño EN,Said-Fernández SL,Martínez-Rodríguez HG

    更新日期:2015-01-28 00:00:00

  • Evidence that phospholipase C is involved in the antitumour action of NSC768313, a new thieno[2,3-b]pyridine derivative.

    abstract:BACKGROUND:The thieno[2,3-b]pyridines were discovered by virtual high throughput screening as potential inhibitors of phospholipase C (PLC) isoforms and showed potent growth inhibitory effects in National Cancer Institute's human tumour cell line panel (NCI60). The mechanism of the anti-proliferative activity of thieno...

    journal_title:Cancer cell international

    pub_type: 杂志文章

    doi:10.1186/s12935-016-0293-6

    authors: Reynisson J,Jaiswal JK,Barker D,D'mello SA,Denny WA,Baguley BC,Leung EY

    更新日期:2016-03-10 00:00:00

  • MiR-20a-5p suppresses tumor proliferation by targeting autophagy-related gene 7 in neuroblastoma.

    abstract:Background:Neuroblastoma (NB) is the most common malignant tumor originating from the extracranial sympathetic nervous system in children. The molecular mechanisms underlying this disease are complex, and not completely understood. Methods:Quantitative real-time PCR (qRT-PCR) was applied to quantify the expression of ...

    journal_title:Cancer cell international

    pub_type: 杂志文章

    doi:10.1186/s12935-017-0499-2

    authors: Yu Y,Zhang J,Jin Y,Yang Y,Shi J,Chen F,Han S,Chu P,Lu J,Wang H,Guo Y,Ni X

    更新日期:2018-01-04 00:00:00

  • The heterogeneous clinical and pathological landscapes of metastatic Braf-mutated colorectal cancer.

    abstract::Colorectal cancer (CRC) is a complex and molecularly heterogeneous disease representing one of the most frequent causes of cancer-related death worldwide. About 8-15% of CRCs harbor a mutation in BRAF gene, a proto-oncogene involved in cell proliferation, differentiation and survival through the MAPK signaling cascade...

    journal_title:Cancer cell international

    pub_type: 杂志文章,评审

    doi:10.1186/s12935-020-1117-2

    authors: Fanelli GN,Dal Pozzo CA,Depetris I,Schirripa M,Brignola S,Biason P,Balistreri M,Dal Santo L,Lonardi S,Munari G,Loupakis F,Fassan M

    更新日期:2020-01-29 00:00:00

  • Identifying the novel key genes in renal cell carcinoma by bioinformatics analysis and cell experiments.

    abstract:Background:Although major driver gene have been identified, the complex molecular heterogeneity of renal cell cancer (RCC) remains unclear. Therefore, more relevant genes need to be identified to explain the pathogenesis of renal cancer. Methods:Microarray datasets GSE781, GSE6344, GSE53000 and GSE68417 were downloade...

    journal_title:Cancer cell international

    pub_type: 杂志文章

    doi:10.1186/s12935-020-01405-6

    authors: Chen Y,Gu D,Wen Y,Yang S,Duan X,Lai Y,Yang J,Yuan D,Khan A,Wu W,Zeng G

    更新日期:2020-07-21 00:00:00

  • Ebp1 expression in benign and malignant prostate.

    abstract:BACKGROUND:ErbB3-binding protein 1 (Ebp1) is a member of the PA2G4 family of proliferation-regulated proteins that is expressed in multiple malignant and non-malignant cells. ErbB3 and other members of the EGFR family have been implicated in cancer progression, it however remains unknown whether Ebp1 participate in pro...

    journal_title:Cancer cell international

    pub_type: 杂志文章

    doi:10.1186/1475-2867-8-18

    authors: Gannon PO,Koumakpayi IH,Le Page C,Karakiewicz PI,Mes-Masson AM,Saad F

    更新日期:2008-11-24 00:00:00

  • Real-time RT-PCR analysis of mRNA decay: half-life of Beta-actin mRNA in human leukemia CCRF-CEM and Nalm-6 cell lines.

    abstract::BACKGROUND: We describe an alternative method to determine mRNA half-life (t1/2) based on the Real-Time RT-PCR procedure. This approach was evaluated by using the beta-actin gene as a reference molecule for measuring of mRNA stability. RESULTS: Human leukemia Nalm-6 and CCRF-CEM cells were treated with various concent...

    journal_title:Cancer cell international

    pub_type: 杂志文章

    doi:10.1186/1475-2867-2-1

    authors: Leclerc GJ,Leclerc GM,Barredo JC

    更新日期:2002-03-07 00:00:00

  • Oncogenic UBE3C promotes breast cancer progression by activating Wnt/β-catenin signaling.

    abstract:BACKGROUND:Breast cancer (BrCa) is the most common female malignancy worldwide and has the highest morbidity among all cancers in females. Unfortunately, the mechanisms of BrCa growth and metastasis, which lead to a poor prognosis in BrCa patients, have not been well characterized. METHODS:Immunohistochemistry (IHC) w...

    journal_title:Cancer cell international

    pub_type: 杂志文章

    doi:10.1186/s12935-020-01733-7

    authors: Hang C,Zhao S,Wang T,Zhang Y

    更新日期:2021-01-06 00:00:00

  • Implications of HIF-1α in the tumorigenesis and progression of pancreatic cancer.

    abstract::Pancreatic cancer is one of the leading causes of cancer-related deaths worldwide and is characterized by highly hypoxic tumor microenvironment. Hypoxia-inducible factor-1 alpha (HIF-1α) is a major regulator of cellular response to changes in oxygen concentration, supporting the adaptation of tumor cells to hypoxia in...

    journal_title:Cancer cell international

    pub_type: 杂志文章,评审

    doi:10.1186/s12935-020-01370-0

    authors: Jin X,Dai L,Ma Y,Wang J,Liu Z

    更新日期:2020-06-24 00:00:00

  • Apigenin promotes apoptosis, inhibits invasion and induces cell cycle arrest of T24 human bladder cancer cells.

    abstract:BACKGROUND:Apigenin (4',5,7-trihydroxyflavone) was recently shown effective in inhibiting several cancers. The aim of this study was to investigate the effect and mechanism of apigenin in the human bladder cancer cell line T24 for the first time. METHODS:T24 cells were treated with varying concentrations and time of a...

    journal_title:Cancer cell international

    pub_type: 杂志文章

    doi:10.1186/1475-2867-13-54

    authors: Zhu Y,Mao Y,Chen H,Lin Y,Hu Z,Wu J,Xu X,Xu X,Qin J,Xie L

    更新日期:2013-06-01 00:00:00

  • HMGB1 mediates lipopolysaccharide-induced inflammation via interacting with GPX4 in colon cancer cells.

    abstract:Background:Inflammation is one of a main reason for colon cancer progression and poor prognosis. The high-mobility group box-1 (HMGB1) and glutathione peroxidase 4 (GPX4) are responsible for inflammation, but the relationship between HMGB1 and GPX4 remains unknown about inflammation in colon cancer. Methods:RT-qPCR wa...

    journal_title:Cancer cell international

    pub_type: 杂志文章

    doi:10.1186/s12935-020-01289-6

    authors: Yang Y,Yang L,Jiang S,Yang T,Lan J,Lei Y,Tan H,Pan K

    更新日期:2020-05-29 00:00:00

  • LBX2-AS1 up-regulated by NFIC boosts cell proliferation, migration and invasion in gastric cancer through targeting miR-491-5p/ZNF703.

    abstract:Background:The crucial role of long non-coding RNAs (lncRNAs) has been certified in human cancers. The lncRNAs with abnormal expressions could act as tumor inhibitors or oncogenes in the advancement of tumors. LBX2-AS1 was once reported to accelerate esophageal squamous cell carcinoma. Nonetheless, its function in gast...

    journal_title:Cancer cell international

    pub_type: 杂志文章

    doi:10.1186/s12935-020-01207-w

    authors: Xu G,Zhang Y,Li N,Wu Y,Zhang J,Xu R,Ming H

    更新日期:2020-04-26 00:00:00

  • SOX2OT knockdown derived changes in mitotic regulatory gene network of cancer cells.

    abstract:Background:SOX2 overlapping transcript (SOX2OT) is a long non-coding RNA, over-expressed in human tumor tissues and embryonic cells. Evidences support its function in the cell cycle; however there is no clear mechanism explaining its function in cell proliferation regulation. Here we investigated cancer cell response t...

    journal_title:Cancer cell international

    pub_type: 杂志文章

    doi:10.1186/s12935-018-0618-8

    authors: Saghaeian Jazi M,Samaei NM,Mowla SJ,Arefnezhad B,Kouhsar M

    更新日期:2018-09-05 00:00:00

  • Functional promoter -765 G > C variant in COX-2 gene is associated with the susceptibility of breast cancer in Chinese Han women.

    abstract:BACKGROUND:Cyclooxygenase (COX) is a rate-limiting enzyme in prostaglandins synthesis which exists in two isoforms, COX-1 and COX-2. Over-expression of COX-2 was considered to increase the proliferation and enhance the invasiveness of breast cancer cells. It was suggested that genetic variations in COX-2 could influenc...

    journal_title:Cancer cell international

    pub_type: 杂志文章

    doi:10.1186/1475-2867-14-38

    authors: Gao J,Kang HF,Ma XB,Tang W,Liu D,Zhao Y,Zhang SQ,Guan HT,Lin S,Ren HT,Wang XJ,Dai ZJ

    更新日期:2014-05-06 00:00:00

  • Ubiquitin ligase CHAF1B induces cisplatin resistance in lung adenocarcinoma by promoting NCOR2 degradation.

    abstract:Background:Lung cancer is the most common malignant tumor in the world. The Whole-proteome microarray showed that ubiquitin ligase chromatin assembly factor 1 subunit B (CHAF1B) expression in A549/DDP cells is higher than in A549 cells. Our study explored the molecular mechanism of CHAF1B affecting cisplatin resistance...

    journal_title:Cancer cell international

    pub_type: 杂志文章

    doi:10.1186/s12935-020-01263-2

    authors: Gong L,Hu Y,He D,Zhu Y,Xiang L,Xiao M,Bao Y,Liu X,Zeng Q,Liu J,Zhou M,Zhou Y,Cheng Y,Zhang Y,Deng L,Zhu R,Lan H,Cao K

    更新日期:2020-05-25 00:00:00

  • SENP1-mediated deSUMOylation of USP28 regulated HIF-1α accumulation and activation during hypoxia response.

    abstract:Background:The ubiquitin-specific protease 28 (USP28) is an oncogenic deubiquitinase, which plays a critical role in tumorigenesis via antagonizing the ubiquitination and degradation of tumor suppressor protein FBXW7-mediated oncogenic substrates. USP28 controls hypoxia-dependent angiogenesis and metastasis by preventi...

    journal_title:Cancer cell international

    pub_type: 杂志文章

    doi:10.1186/s12935-018-0722-9

    authors: Du SC,Zhu L,Wang YX,Liu J,Zhang D,Chen YL,Peng Q,Liu W,Liu B

    更新日期:2019-01-03 00:00:00

  • Silenced lncRNA SNHG14 restrains the biological behaviors of bladder cancer cells via regulating microRNA-211-3p/ESM1 axis.

    abstract:BACKGROUND:Bladder cancer (BCa) is a malignant tumor that occurs on the mucosa of the bladder, in which dysregulated long non-coding RNAs (lncRNAs) are involved. This study investigated the effect of lncRNA small nucleolar RNA host gene 1 (SNHG14) on the biological characteristics of BCa cells from microRNA (miR)-211-3...

    journal_title:Cancer cell international

    pub_type: 杂志文章

    doi:10.1186/s12935-020-01717-7

    authors: Feng R,Li Z,Wang X,Ge G,Jia Y,Wu D,Ji Y,Wang C

    更新日期:2021-01-22 00:00:00

  • Circular RNA and tumor microenvironment.

    abstract::Circular RNAs (circRNAs) are small non-coding RNAs with a unique ring structure and play important roles as gene regulators. Disturbed expressions of circRNAs is closely related to varieties of pathological processes. The roles of circRNAs in cancers have gained increasing concerns. The communications between the canc...

    journal_title:Cancer cell international

    pub_type: 杂志文章,评审

    doi:10.1186/s12935-020-01301-z

    authors: Song H,Liu Q,Liao Q

    更新日期:2020-06-03 00:00:00