Abstract:
Background:Transgelin, an actin-binding protein, is associated with cytoskeleton remodeling. Findings from our previous studies demonstrated that transgelin was up-regulated in node-positive colorectal cancer (CRC) versus node-negative disease. Over-expression of TAGLN affected the expression of 256 downstream transcripts and increased the metastatic potential of colon cancer cells in vitro and in vivo. This study aims to explore the mechanisms through which transgelin participates in the metastasis of colon cancer cells. Methods:Immunofluorescence and immunoblotting analysis were used to determine the cellular localization of endogenous and exogenous transgelin in colon cancer cells. Co-immunoprecipitation and subsequently high-performance liquid chromatography/tandem mass spectrometry were performed to identify the proteins that were potentially interacting with transgelin. The 256 downstream transcripts regulated by transgelin were analyzed with bioinformatics methods to discriminate the specific key genes and signaling pathways. The Gene-Cloud of Biotechnology Information (GCBI) tools were used to predict the potential transcription factors (TFs) for the key genes. The predicted TFs corresponded to the proteins identified to interact with transgelin. The interaction between transgelin and the TFs was verified by co-immunoprecipitation and immunofluorescence. Results:Transgelin was found to localize in both the cytoplasm and nucleus of the colon cancer cells. Approximately 297 proteins were identified to interact with transgelin. The overexpression of TAGLN led to the differential expression of 184 downstream genes. Network topology analysis discriminated seven key genes, including CALM1, MYO1F, NCKIPSD, PLK4, RAC1, WAS and WIPF1, which are mostly involved in the Rho signaling pathway. Poly (ADP-ribose) polymerase-1 (PARP1) was predicted as the unique TF for the key genes and concurrently corresponded to the DNA-binding proteins potentially interacting with transgelin. The interaction between PARP1 and transgelin in human RKO colon cancer cells was further validated by immunoprecipitation and immunofluorescence assays. Conclusions:Our results suggest that transgelin binds to PARP1 and regulates the expression of downstream key genes, which are mainly involved in the Rho signaling pathway, and thus participates in the metastasis of colon cancer.
journal_name
Cancer Cell Intjournal_title
Cancer cell internationalauthors
Lew ZX,Zhou HM,Fang YY,Ye Z,Zhong W,Yang XY,Yu Z,Chen DY,Luo SM,Chen LF,Lin Ydoi
10.1186/s12935-020-01461-ysubject
Has Abstractpub_date
2020-08-03 00:00:00pages
366issn
1475-2867pii
1461journal_volume
20pub_type
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journal_title:Cancer cell international
pub_type: 杂志文章
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journal_title:Cancer cell international
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journal_title:Cancer cell international
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journal_title:Cancer cell international
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journal_title:Cancer cell international
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journal_title:Cancer cell international
pub_type: 杂志文章
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journal_title:Cancer cell international
pub_type: 杂志文章
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journal_title:Cancer cell international
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journal_title:Cancer cell international
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更新日期:2017-09-26 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-019-1004-x
更新日期:2019-11-06 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-018-0710-0
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journal_title:Cancer cell international
pub_type: 杂志文章
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journal_title:Cancer cell international
pub_type: 杂志文章
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journal_title:Cancer cell international
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更新日期:2018-12-27 00:00:00
abstract::[This corrects the article DOI: 10.1186/s12935-017-0407-9.]. ...
journal_title:Cancer cell international
pub_type: 已发布勘误
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更新日期:2020-07-17 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
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abstract::BACKGROUND: The aim of this work was to investigate in vitro the putative role of EGR-1 in the growth of glioma cells. EGR-1 expression was examined during the early passages in vitro of 17 primary cell lines grown from 3 grade III and from 14 grade IV malignant astrocytoma explants. The explanted tumors were genetica...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-4-1
更新日期:2004-01-07 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-018-0545-8
更新日期:2018-04-02 00:00:00
abstract:BACKGROUND:Natural killer (NK) cells eliminate virus-infected and tumor cells through the release of perforins and granzymes; they also produce Interferon gamma (IFN-γ) and Tumor necrosis factor alpha (TNF-α), which induce apoptosis in target cells. Many tumors express Heme oxygenase 1 (HO-1), and this expression has b...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-014-0100-1
更新日期:2014-10-01 00:00:00
abstract:Background:Accumulating studies showed that long noncoding RNAs (lncRNAs) played vital roles in cancer progression. LncRNA MIR4435-2HG was proved to act as an oncogene in various tumors. However, the underlying function of MIR4435-2HG in ovarian cancer (OC) remains unclear. Methods:The expression levels of MIR4435-2HG...
journal_title:Cancer cell international
pub_type: 杂志文章
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更新日期:2020-05-01 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-019-0822-1
更新日期:2019-04-16 00:00:00
abstract:Background:Dysregulation of long non-coding RNAs (lncRNAs) results in development of human diseases including hepatocellular carcinoma (HCC). Although several HCC related lncRNAs have been reported, the biological functions of many lncRNAs during the development of HCC remains unknown. Methods:The expression of ST8SIA...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-01325-5
更新日期:2020-06-11 00:00:00
abstract:Background:This study aims to investigate the role of microRNA-92a (miR-92a) in metastasis of osteosarcoma (OS) cells in vivo and in vitro by regulatingTCF21 with the transmission of bone marrow derived mesenchymal stem cells (BMSCs). Methods:BMSCs were isolated, purified and cultured from healthy adult bone marrow ti...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-019-0741-1
更新日期:2019-02-12 00:00:00