Abstract：
:Crohn's disease (CD) is characterized by compromised immune tolerance to the intestinal commensal microbiota, intestinal barrier inflammation, and hyperplasia of creeping fat (CF) and mesenteric adipose tissue (AT), which seems to be directly related to disease activity. Gut microbiota dysbiosis might be a determining factor in CD etiology, manifesting as a low microbial diversity and a high abundance of potentially pathogenic bacteria. We tested the hypothesis that CF is a reservoir of bacteria through 16S-rRNA sequencing of several AT depots of patients with active and inactive disease and controls. We found a microbiome signature within CF and mesenteric AT from patients, but not in subcutaneous fat. We failed to detect bacterial DNA in any fat depot of controls. Proteobacteria was the most abundant phylum in both CF and mesenteric AT, and positively correlated with fecal calprotectin/C-reactive protein. Notably, the clinical status of patients seemed to be related to the microbiome signature, as those with the inactive disease showed a reduction in the abundance of pathogenic bacteria. Predictive functional profiling revealed many metabolic pathways including lipopolysaccharide biosynthesis and sulfur metabolism overrepresented in active CD relative to that in inactive CD. Our findings demonstrate that microbiota dysbiosis associated with CD pathophysiology is reflected in AT and might contribute to disease severity.
journal_name
J Clin Medjournal_title
Journal of clinical medicineauthors
Serena C,Queipo-Ortuño M,Millan M,Sanchez-Alcoholado L,Caro A,Espina B,Menacho M,Bautista M,Monfort-Ferré D,Terrón-Puig M,Núñez-Roa C,Maymó-Masip E,Rodriguez MM,Tinahones FJ,Espin E,Martí M,Fernández-Veledo S,Vendrell Jdoi
10.3390/jcm9082448subject
Has Abstractpub_date
2020-07-31 00:00:00issue
8issn
2077-0383pii
jcm9082448journal_volume
9pub_type
杂志文章abstract：:To define the role of spleen stiffness (SS) and liver stiffness (LS) in myelofibrosis and other Philadelphia (Ph)-negative myeloproliferative neoplasms (MPNs), we studied, by ultrasonography (US) and elastography (ES), 70 consecutive patients with myelofibrosis (MF) (no.43), essential thrombocythemia (ET) (no.10), and...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9072149
更新日期：2020-07-08 00:00:00
abstract：:Several conditions can lead to the development of a subchondral cyst. The mechanism by which the cysts form, their location, and their severity depend on the underlying pathology, although the exact pathogenesis is not fully elucidated. Treatment options vary according to the location of the cyst, with less invasive p...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9051358
更新日期：2020-05-06 00:00:00
abstract：:Considering the progress made in the management of sickle cell disease during the past 30 years, along with the excellent results obtained with hematopoietic stem cell transplantation (SCT), it is important to reexamine why, who, when and how to recommend allogeneic SCT in children with sickle cell disease. While sick...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm8101523
更新日期：2019-09-22 00:00:00
abstract：:In the past two decades, multiple studies have been undertaken to elucidate the genetic cause of the predisposition to mismatch repair (MMR)-proficient nonpolyposis colorectal cancer (CRC). Here, we present the proposed candidate genes according to their involvement in specific pathways considered relevant in heredita...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm9061954
更新日期：2020-06-23 00:00:00
abstract：:Diabetes in pregnancy, both preexisting type 1 or type 2 and gestational diabetes, is a highly prevalent condition, which has a great impact on maternal and fetal health, with short and long-term implications. Gestational Diabetes Mellitus (GDM) is a condition triggered by metabolic adaptation, which occurs during the...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm7020011
更新日期：2018-01-25 00:00:00
abstract：:Neovascular age related macular degeneration (nAMD) leads to severe vision loss worldwide and is characterized by the formation of choroidal neovascularization (CNV) and fibrosis. In the current study, we aimed to investigate the effect of blockade for platelet derived growth factor receptor-β (PDGFR-β) on the formati...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9072242
更新日期：2020-07-15 00:00:00
abstract：OBJECTIVE:Clinical presentation of patients with mitral paravalvular leakage (PVL) varies from asymptomatic to heart failure related with hemolytic anemia or pulmonary hypertension. We aimed to investigate the structural and functional characteristics of mitral PVL by multimodal imaging and their association with the s...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm10020222
更新日期：2021-01-10 00:00:00
abstract：:Early diagnosis and timely management of tooth or dental material wear is imperative to avoid extensive restorations. Previous studies suggested different methods for tooth wear assessment, but no study has developed a three-dimensional (3D) superimposition technique applicable in cases where tooth surfaces, other tha...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9123937
更新日期：2020-12-04 00:00:00
abstract：INTRODUCTION:Telemedicine is believed to be helpful in managing patients suffering from chronic diseases, in particular elderly patients with numerous accompanying conditions. This was the basis for the "GERIATRICS and e-Technology (GER-e-TEC) study", which was an experiment involving the use of the smart MyPredi™ e-pl...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9123836
更新日期：2020-11-26 00:00:00
abstract：BACKGROUND:Our study aims to perform a meta-analysis of benefits and risks associated with the use of non-vitamin K oral anticoagulants (NOAC) versus vitamin K antagonists (VKA) in patients with a percutaneous coronary intervention (PCI) with a particular focus on the combination type: dual vs. dual antithrombotic ther...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9041120
更新日期：2020-04-14 00:00:00
abstract：:In recent years, the genetic architecture of dilated cardiomyopathy (DCM) has been more thoroughly elucidated. However, there is still insufficient knowledge on the modifiers and regulatory principles that lead to the failure of myocardial function. The current study investigates the association of epigenome-wide DNA ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9051499
更新日期：2020-05-16 00:00:00
abstract：:Despite the overall success of cochlear implantation, language outcomes remain suboptimal and subject to large inter-individual variability. Early auditory rehabilitation techniques have mostly focused on low-level sensory abilities. However, a new body of literature suggests that cognitive operations are critical for...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9041058
更新日期：2020-04-08 00:00:00
abstract：:Restless legs syndrome (RLS) is highly prevalent among pregnant women. In the present study, a neurological-obstetrical sample of 561 postpartum women was retrospectively screened for RLS symptoms during pregnancy and in the first 12 weeks postpartum. The first screening took place within 1 to 6 days of delivery (T0) ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9093046
更新日期：2020-09-21 00:00:00
abstract：:Traumatic brain injury is not a discrete event but an unfolding sequence of damage to the central nervous system. Not only the acute phase but also the subacute and chronic period after injury, i.e., during inpatient rehabilitation, is characterized by multiple neurotransmitter alterations, cellular dysfunction, and m...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm4091815
更新日期：2015-09-22 00:00:00
abstract：:Oxidative stress arises when cellular antioxidant defences become overwhelmed by a surplus generation of reactive oxygen species (ROS). Once this occurs, many cellular biomolecules such as DNA, lipids, and proteins become susceptible to free radical-induced oxidative damage, and this may consequently lead to cellular ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm6110100
更新日期：2017-10-27 00:00:00
abstract：:The widely varying therapeutic response of patients with inflammatory bowel disease (IBD) continues to raise questions regarding the unclarified heterogeneity of pathological mechanisms promoting disease progression. While biomarkers for the differentiation of Crohn's disease (CD) versus ulcerative colitis (UC) have b...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9051360
更新日期：2020-05-06 00:00:00
abstract：:COVID-19 has catastrophically affected the world's panoramic view of human well-being in terms of healthcare and management. With the increase in the number of cases worldwide, neurological symptoms and psychological illnesses from COVID-19 have increasingly upsurged. Mental health illness and affective disorders, inc...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm9082624
更新日期：2020-08-13 00:00:00
abstract：:: Direct energy deposition (DED) technology has gained increasing attention as a new implant surface technology that replicates the porous structure of natural bones facilitating osteoblast colonization and bone ingrowth. However, concerns have arisen over osteolysis or chronic inflammation that could be caused by Cob...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9020478
更新日期：2020-02-09 00:00:00
abstract：:Endometrial cancer (EC) is the sixth most common cancer in women worldwide and its mortality is directly associated with the presence of poor prognostic factors driving tumor recurrence. Stratification systems are based on few molecular, and mostly clinical and pathological parameters, but these systems remain inaccur...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm9061900
更新日期：2020-06-17 00:00:00
abstract：:Endometriosis concerns more than 10% of women of reproductive age, frequently leading to chronic pelvic pain. Repetitive transcranial magnetic stimulation (rTMS) over the primary motor cortex (M1) induces an analgesic effect. This effect on chronic pelvic pain is yet to be evaluated. The objective of this study was to...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm8040508
更新日期：2019-04-13 00:00:00
abstract：:Often discovered at an advanced stage, ovarian cancer progresses to peritoneal carcinoma, which corresponds to the invasion of the serosa by multiple tumor implants. The current treatment is based on the combination of chemotherapy and tumor cytoreduction surgery. Despite the progress and standardization of surgical t...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9041185
更新日期：2020-04-21 00:00:00
abstract：:α-Klotho is a known anti-aging protein that exerts diverse physiological effects, including phosphate homeostasis. Klotho expression occurs predominantly in the kidney and is significantly decreased in patients with chronic kidney disease. However, changes in serum klotho levels and impacts of klotho on outcomes among...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9061834
更新日期：2020-06-12 00:00:00
abstract：:The focus of the presented work was to isolate and characterize circulating endometrial cells (CECs) enriched from peripheral blood (PB) of patients with diagnosed endometriosis. The molecular characteristics of CECs could be supportive for an understanding of endometriosis pathogenesis and treatment decisions in the ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm8111938
更新日期：2019-11-11 00:00:00
abstract：:Hybrid transplantation of skeletal muscle-derived multipotent stem cells (Sk-MSCs) and bioabsorbable polyglyconate (PGA) felt was studied as a novel regeneration therapy for the transected recurrent laryngeal nerve (RLN). Sk-MSCs were isolated from green fluorescence protein transgenic mice and then expanded and trans...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm7090276
更新日期：2018-09-12 00:00:00
abstract：:We aimed to examine the usefulness of serum glutathione peroxidase 3 (GPx3) as a biomarker of lung cancer recurrence after complete resection. We prospectively collected serial serum samples at the baseline, as well as 3, 6 and 12 months after surgery from complete resection cases in 2013. GPx3 levels were measured by...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9123801
更新日期：2020-11-24 00:00:00
abstract：:Although skin manifestations are quite common in systemic sclerosis (SSc), a link between SSc and psoriasis (PsO) has been poorly investigated. We assessed the Clalit medical database in a cohort study to compare the prevalence of PsO between SSc-patients and SSc-free controls. We also evaluated the SSc-related autoan...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm8040521
更新日期：2019-04-16 00:00:00
abstract：:Tadalafil 5 mg represents the standard for men with Erectile dysfunction (ED) and lower urinary tract symptoms (LUTS)/benign prostatic enlargement (BPE). We carried out an observational trial aiming to assess the efficacy and safety of Tadalafil compared with Tadalafil plus Tamsulosin. Seventy-five patients complainin...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm8081126
更新日期：2019-07-29 00:00:00
abstract：:The prognostic significance of sarcopenia in unresectable advanced esophageal cancer remains unclear. Our study retrospectively evaluated 176 consecutive Japanese patients with esophageal squamous cell carcinoma who had been diagnosed with unresectable advanced cancer in Aichi Cancer Center Hospital between January 20...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm8101647
更新日期：2019-10-09 00:00:00
abstract：:In excess of three quarters of patients with psoriatic arthritis (PsA) have preceding psoriasis (PsO), which offers a clinical biomarker for the recognition of early PsA. Numerous surveys have shown a remarkably high frequency of clinically occult musculoskeletal symptoms in psoriasis patients. Imaging studies, partic...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm9103262
更新日期：2020-10-12 00:00:00
abstract：:Acute myeloid leukemia is mainly characterized by a complex and dynamic genomic instability. Next-generation sequencing has significantly improved the ability of diagnostic research to molecularly characterize and stratify patients. This detailed outcome allowed the discovery of new therapeutic targets and predictive ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm9030802
更新日期：2020-03-16 00:00:00
