Abstract：
:The focus of the presented work was to isolate and characterize circulating endometrial cells (CECs) enriched from peripheral blood (PB) of patients with diagnosed endometriosis. The molecular characteristics of CECs could be supportive for an understanding of endometriosis pathogenesis and treatment decisions in the future. MATERIAL AND METHODS:Blood samples (n = 423) were tested for CECs presence. Subsequently, gene expression analysis (GEA) was carried out for CECs. In parallel, CECs presence and characteristics were tested during menstrual cycle (MC) phases in 11 patients. CECs were enriched by size-based separation. RESULTS:CECs were present in 78.4% of the tested blood samples. In line with the revised American Fertility Society (rAFS) classification, CECs presence was confirmed in all the acknowledged endometriosis stages: minimal, mild, moderate, and severe. Surprisingly, CECs negativity rate was also reported for severe disease in 21.1% of cases. The CECs captured during MC phases displayed different cytomorphology, including epithelial, stromal, and stem cell-like characteristics. The highest CECs numbers were detected in the mid-secretory phase of MC, which corresponds to uterine lining decidualization. CECs captured during mid-secretory periods expressed genes KRT18, NANOG, and VIM in higher amounts when compared to the proliferative phase of MC, where genes KRT19 and ESR1 were mostly elevated. GEA of the super-positive CECs samples (1000 CECs/8 mL PB) revealed high expression of genes KRT18, VIM, NANOG, and FLT1. The expression of these genes was also elevated in the endometriosis tissue samples and endometrioma. CONCLUSION:The panel of the identified CEC genes could be tested in a prospective manner to confirm the role of CECs in endometriosis pathogenesis and diagnostics.
journal_name
J Clin Medjournal_title
Journal of clinical medicineauthors
Pospisilova E,Kiss I,Souckova H,Tomes P,Spicka J,Matkowski R,Jedryka M,Ferrero S,Bobek V,Kolostova Kdoi
10.3390/jcm8111938subject
Has Abstractpub_date
2019-11-11 00:00:00issue
11issn
2077-0383pii
jcm8111938journal_volume
8pub_type
杂志文章abstract：:Permanent hypoparathyroidism, a feared thyroidectomy complication, leads to significant patient morbidity, medical treatment, and monitoring. This study explores whether preoperative high-dose vitamin D loading decreases the incidence of permanent hypoparathyroidism. In a subgroup analysis, the study examines the pred...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm10030442
更新日期：2021-01-24 00:00:00
abstract：:Ocular surgery is one of the most performed medical procedures in the world. Its limitation or suspension, recursively extended over time, could be associated with a significant increase in the number of blind people worldwide. Indeed, cataract causes more than half of all cases of visual impairment in those countries...
journal_title：Journal of clinical medicine
pub_type： 社论
doi：10.3390/jcm9051403
更新日期：2020-05-09 00:00:00
abstract：:We investigated the prognostic influence of androgen receptor (AR) on breast cancer. AR status was assessed using immunohistochemistry with tissue microarrays from 395 operable primary breast cancer patients who received curative surgery. The Kaplan-Meier estimator was used to analyze the survival rates and a log-rank...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9041083
更新日期：2020-04-10 00:00:00
abstract：BACKGROUND:Invasive fungal disease (IFD) is common in solid organ transplant (SOT) recipients and contributes to high morbidity and mortality. Although kidney transplantation (KT) is a commonly performed SOT, data on the risk factors for IFD-related mortality are limited. METHODS:A 1:2 retrospective case-control study...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9061824
更新日期：2020-06-11 00:00:00
abstract：:To define the role of spleen stiffness (SS) and liver stiffness (LS) in myelofibrosis and other Philadelphia (Ph)-negative myeloproliferative neoplasms (MPNs), we studied, by ultrasonography (US) and elastography (ES), 70 consecutive patients with myelofibrosis (MF) (no.43), essential thrombocythemia (ET) (no.10), and...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9072149
更新日期：2020-07-08 00:00:00
abstract：:Sarcoidosis is a systemic disease of unknown etiology characterized by the presence of noncaseating granulomas that can occur in any organ, most commonly the lungs. Early and accurate diagnosis of sarcoidosis remains challenging because initial presentations may vary, many patients are asymptomatic, and there is no si...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm9082363
更新日期：2020-07-24 00:00:00
abstract：:Healthcare systems in the developed world are struggling with the demand of emergency room presentations; the study of the factors driving such demand is of fundamental importance. From a database of all emergency medical admissions (66,933 episodes in 36,271 patients) to St James' Hospital, Dublin, Ireland, over 12 y...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm3041220
更新日期：2014-10-29 00:00:00
abstract：:Multidrug resistant Gram-Negative Bacterial Infections (MR-GNBI) are an increasing cause of mortality in acute myeloid leukemia (AML), compromising the success of antineoplastic therapy. We prospectively explored a novel strategy, including mandatory fluoroquinolone prophylaxis, weekly surveillance cultures (SC) and t...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm8111985
更新日期：2019-11-15 00:00:00
abstract：:Actinic cheilitis (AC) is one of the most frequent pathologies to affect the lips. Studies show that the most commonplace oral malignancy, squamous cell carcinoma (SCC), often emerges from AC lesions. Invasive diagnostic techniques performed on the lips carry a high risk of complications, but reflectance confocal micr...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9061987
更新日期：2020-06-25 00:00:00
abstract：BACKGROUND:Nine percent of all cases of tuberculosis are bone and joint tuberculosis (BJTB). BJTB occurs in two main forms: spinal (STB) and extraspinal (ESTB). The aim of this study was to compare STB with ESTB in terms of diagnosis, treatment and outcomes. METHODS:We collected demographic, clinical, microbiological,...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9082529
更新日期：2020-08-05 00:00:00
abstract：INTRODUCTION:The inherent immunosuppression of uremia increases the susceptibility of hemodialysis patients to infection. There is still limited evidence on hemodialysis patients and COVID-19. The clinical and analytical spectrum and treatment responses and mortality are poorly characterized. MATERIAL AND METHODS:Clin...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9072264
更新日期：2020-07-16 00:00:00
abstract：:Torsional abnormalities of the femur represent a significant risk factor for patellar instability or patellofemoral complaints. Although their clinical implication has been demonstrated, there is still a debate going on about different aspects. These include, especially, the various methods of measurements with a wide...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm10030474
更新日期：2021-01-27 00:00:00
abstract：BACKGROUND:Hyponatraemia is associated with increased morbidity, increased mortality and is frequently hospital-acquired due to inappropriate administration of hypotonic fluids. Despite several attempts to minimise the risk, knowledge is lacking as to whether inappropriate prescribing practice continues to be a concern...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9092790
更新日期：2020-08-29 00:00:00
abstract：:This phase II randomized controlled trial tested whether intracoronary autologous CD34+ cell therapy could further improve left ventricular (LV) systolic function in patients with diffuse coronary artery disease (CAD) with relatively preserved LV ejection fraction (defined as LVEF >40%) unsuitable for coronary interve...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9041043
更新日期：2020-04-07 00:00:00
abstract：:Diabetes mellitus is one of the most prevalent chronic diseases worldwide. Diabetic patients are at risk of developing cataract and present for surgery at an earlier age than non-diabetics. The aim of this study was to review the problems associated with cataract surgery in a diabetic patient. Corneal complications in...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm8050716
更新日期：2019-05-20 00:00:00
abstract：BACKGROUND:The Dyskinesia Impairment Scale (DIS) is a new assessment scale for dystonia and choreoathetosis in children and youth with dyskinetic cerebral palsy. Today, the Burke-Fahn-Marsden Dystonia Rating Scale (BFM) is mostly used to assess dystonia in children with inherited dystonia. The aim of this study was to ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9082597
更新日期：2020-08-11 00:00:00
abstract：:Most studies on well-being during the COVID-19 pandemic have focused on the mental health of the general population; far less attention has been given to more specific populations, such as patients with mental illness. Indeed, it is important to examine the psychiatric population, given its vulnerability. The present ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9113787
更新日期：2020-11-23 00:00:00
abstract：BACKGROUND:Prostate cancer (PCa) is characterized by significant heterogeneity in its molecular, genomic, and immunologic characteristics. METHODS:Whole transcriptome RNAseq data from The Cancer Genome Atlas of prostate adenocarcinomas (n = 492) was utilized. The immune microenvironment was characterized using the CIB...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9061973
更新日期：2020-06-24 00:00:00
abstract：:The relationship between serum uric acid (SUA) and hypertension has been a subject of increasing interest since the 1870 discovery by Frederick Akbar Mahomed. Several epidemiological studies have shown a strong association between high SUA levels and the presence or the development of hypertension. Genetic analyses ha...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm10030448
更新日期：2021-01-24 00:00:00
abstract：:Both pre-gestational maternal obesity (PGMO) and excessive gestational weight gain (EGWG) increase the risk of gestational diabetes mellitus (GDM). Here, we conducted a retrospective study to comparatively examine the relation between fetal birth weight (FW) and placental weight (PW) in PGMO (n = 100) compared to EGWG...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9113530
更新日期：2020-10-31 00:00:00
abstract：:The idea of right heart catheterization (RHC) grew in the milieu of modern thinking about the cardiovascular system, influenced by the experiments of William Harvey, which were inspired by the treatises of Greek philosophers like Aristotle and Gallen, who made significant contributions to the subject. RHC was first di...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm8091331
更新日期：2019-08-28 00:00:00
abstract：:Differing beliefs about the acceptability of living-donor kidney transplants (LDKTs) have been proposed as explaining age, ethnic and socioeconomic disparities in their uptake. We investigated whether certain patient groups hold beliefs incompatible with LDKTs. This questionnaire-based case-control study was based at ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9010031
更新日期：2019-12-21 00:00:00
abstract：:Atherosclerosis is a progressive, inflammatory cardiovascular disorder characterized by the development of lipid-filled plaques within arteries. Endothelial cell dysfunction in the walls of blood vessels results in an increase in vascular permeability, alteration of the components of the extracellular matrix, and rete...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm8111856
更新日期：2019-11-02 00:00:00
abstract：:Injury is a major cause of worldwide child mortality. This retrospective nationwide study aimed to evaluate the characteristics of paediatric injured patients in Japan and their in-hospital mortality trends from 2009 to 2018. Injured patients aged <17 years were enrolled. Data were extracted from the Japan Trauma Data...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9103273
更新日期：2020-10-12 00:00:00
abstract：:Pediatric Adamantinomatous Craniopharyngiomas (ACPs) are histologically benign brain tumors that often follow an aggressive clinical course. Their suprasellar location leaves them in close proximity to critical neurological and vascular structures and often results in significant neuroendocrine morbidity. Current trea...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm9020519
更新日期：2020-02-14 00:00:00
abstract：:In the statin era, the incidence of atherosclerotic cardiovascular diseases (ASCVD) in patients with familial hypercholesterolemia (FH) has not been updated. We aimed to determine the incidence of ASCVD in patients with FH-phenotype (FH-P) and to compare it with that of normal low-density lipoprotein cholesterol (LDL-...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm8071080
更新日期：2019-07-23 00:00:00
abstract：:The investigation of the potential association between ischemic stroke and subclinical atrial fibrillation (SCAF) is important for secondary prevention. We aimed to determine whether SCAF can be predicted by atrial substrate measurement with P wave signal-averaged electrocardiography (SAECG). We recruited 125 consecut...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9041134
更新日期：2020-04-15 00:00:00
abstract：BACKGROUND:Inflammation is often considered relating to pediatric obstructive sleep apnea (OSA). We conducted a study investigating cytokines, including Il-17 and Il-23, in children with OSA before and after adenotonsillectomy (T&A), compared with controls. METHODS:Children with OSA between age 4 and 12 receiving T&A ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9041028
更新日期：2020-04-05 00:00:00
abstract：BACKGROUND:There is a paucity of head-to-head comparisons of the efficacy and harms of pharmacological treatments for systemic sclerosis-related interstitial lung disease (SSc-ILD). METHODS:We conducted a network meta-analysis (NMA) in order to compare the effects of different treatments with the placebo on change in ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm9082560
更新日期：2020-08-07 00:00:00
abstract：:Abnormalities in body composition are associated with poor prognosis in cancer patients. We investigated the association between body composition and 90-day mortality in cancer patients who developed septic shock. We included consecutive septic shock patients with active cancer from 2010 to 2017. The muscle area at th...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm8101583
更新日期：2019-10-02 00:00:00
