Abstract:
:Modifications to the surface chemistry, charge, and hydrophilicity/hydrophobicity of nanoparticles are applicable approaches to the alterations of the in vivo fate of intravenously administered nano-sized drug carriers. The objective of this study is to investigate the in vitro and in vivo antitumor efficacies of curcumin PLGA nanoparticles in relation to their surface structural modification via self-assembling coating with unique fungal hydrophobin. The hydophobin-coated curcumin PLGA nanoparticles (HPB PLGA NPs) were obtained by simply soaking curcumin-loaded PLGA nanoparticles (PLGA NPs) in aqueous fungal hydrophobin solution. The in vitro drug release behavior of the HPB PLGA NPS was also tested. The cytotoxicity and cellular uptake of these nanoparticles were determined in HepG2, A549, and Hela cell lines using MTT assay method and CLSM observation. The in vivo antitumor activity was evaluated in Hela tumor xenografted mice model. Compared with the PLGA NPs, the size and zeta potential of the nanoparticles were changed after hydrophobin coating, whereas similar in vitro release pattern was observed. The pharmacodynamics study showed prolonged blood retention of both nano-formulations than that of free curcumin, but no significant difference between the hydrophobin coated and uncoated nanoparticles. It was found that HPB PLGA NPs had increased cytotoxicities, higher cellular uptake, and improved antitumor efficacy. Surface modification of nanoparticles via self-assembling of hydrophobin is a convenient and promising method of changing particle surface physiochemical properties and antitumor performances. Further investigations, especially on tissue distribution, were needed to assess the potential application of the hydrophobin self-assembling coating in nano-drug delivery carriers.
journal_name
AAPS PharmSciTechjournal_title
AAPS PharmSciTechauthors
Sun L,Xu H,Xu JH,Wang SN,Wang JW,Zhang HF,Jia WR,Li LSdoi
10.1208/s12249-020-01698-wsubject
Has Abstractpub_date
2020-06-11 00:00:00pages
171issue
5issn
1530-9932pii
10.1208/s12249-020-01698-wjournal_volume
21pub_type
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journal_title:AAPS PharmSciTech
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journal_title:AAPS PharmSciTech
pub_type: 传,历史文章,新闻
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journal_title:AAPS PharmSciTech
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journal_title:AAPS PharmSciTech
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journal_title:AAPS PharmSciTech
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journal_title:AAPS PharmSciTech
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journal_title:AAPS PharmSciTech
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journal_title:AAPS PharmSciTech
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journal_title:AAPS PharmSciTech
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journal_title:AAPS PharmSciTech
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journal_title:AAPS PharmSciTech
pub_type: 杂志文章,评审
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journal_title:AAPS PharmSciTech
pub_type: 杂志文章
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journal_title:AAPS PharmSciTech
pub_type: 杂志文章,评审
doi:10.1208/s12249-013-0062-y
更新日期:2014-04-01 00:00:00
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journal_title:AAPS PharmSciTech
pub_type: 杂志文章
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journal_title:AAPS PharmSciTech
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journal_title:AAPS PharmSciTech
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journal_title:AAPS PharmSciTech
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journal_title:AAPS PharmSciTech
pub_type: 杂志文章
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journal_title:AAPS PharmSciTech
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journal_title:AAPS PharmSciTech
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更新日期:2019-06-18 00:00:00
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journal_title:AAPS PharmSciTech
pub_type: 杂志文章
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abstract::The purpose of this research was to compare the viscoelastic properties of several neutral and anionic polysaccharide polymers with their mucociliary transport rates (MTR) across explants of ciliated bovine tracheal tissue to identify rheologic parameters capable of predicting the extent of reduction in mucociliary tr...
journal_title:AAPS PharmSciTech
pub_type: 杂志文章
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journal_title:AAPS PharmSciTech
pub_type: 杂志文章
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更新日期:2018-02-01 00:00:00
abstract::The objective of this paper is to assess the number of drug particles or droplets contained in metered dose inhaler (MDI) aerosols. Equations were developed to estimate this. The number of drug particles was estimated to be as high as about 300 million for QVAR solution MDIs and as low as 670,000 for Beclovent MDIs. T...
journal_title:AAPS PharmSciTech
pub_type: 杂志文章
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