当前位置： SCI文献检索 > Viruses-Basel期刊下所有文献 > Successive Passage In Vitro Led to Lower Virulence and Higher Titer of A Variant Porcine Epidemic Diarrhea Virus.

Successive Passage In Vitro Led to Lower Virulence and Higher Titer of A Variant Porcine Epidemic Diarrhea Virus.

Abstract：

:A highly virulent porcine epidemic diarrhea virus (PEDV) appeared in China and spread rapidly to neighbor countries, which have led to great economic losses to the pig industry. In the present study, we isolated a PEDV using Vero cells and serially propagated 100 passages. PEDV SDSX16 was characterized in vitro and in vivo. The viral titers increased to 107.6 TCID50/mL (100th) by serial passages. The spike (S) gene and the whole gene of the SDSX16 virus was fully sequenced to assess the genetic stability and relatedness to previously identified PEDV. Along with successive passage in vitro, there were 18 nucleotides (nt) deletion occurred in the spike (S) gene resulting in a deletion of six amino acids when the SDSX16 strain was passaged to the 64th generation, and this deletion was stable until the P100. However, the ORF1a/b, M, N, E, and ORF3 genes had only a few point mutations in amino acids and no deletions. According to growth kinetics experiments, the SDSX16 deletion strain significantly enhanced its replication in Vero cells since it was passaged to the 64th generation. The animal studies showed that PEDV SDSX16-P10 caused more severe diarrhea and vomiting, fecal shedding, and acute atrophic enteritis than SDSX16-P75, indicating that SDSX16-P10 is enteropathogenic in the natural host, and the pathogenicity of SDSX16 decreased with successive passage in vitro. However, SDSX16-P10 was found to cause lower levels of cytokine expression than SDSX16-P75 using real-time PCR and flow cytometry, such as IL1β, IL6, IFN-β, TNF-α, indicating that SDSX16-P10 might inhibit the expression of cytokines. Our data indicated that successive passage in vitro resulted in virulent attenuation in vivo of the PEDV variant strain SDSX16.

journal_name

Viruses

journal_title

Viruses

authors

Zhao P,Wang S,Chen Z,Yu J,Tang R,Qiu W,Zhao L,Liu Y,Guo X,He H,Xu G,Li J,Wu J

doi

10.3390/v12040391

subject

Has Abstract

pub_date

2020-04-01 00:00:00

issue

4

issn

1999-4915

pii

v12040391

journal_volume

12

pub_type

杂志文章

相关文献

Viruses-Basel文献大全
  • Application of CRISPR/Cas9 Gene Editing System on MDV-1 Genome for the Study of Gene Function.

    abstract：:Marek's disease virus (MDV) is a member of alphaherpesviruses associated with Marek's disease, a highly contagious neoplastic disease in chickens. Complete sequencing of the viral genome and recombineering techniques using infectious bacterial artificial chromosome (BAC) clones of Marek's disease virus genome have ide...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v10060279

    authors： Zhang Y,Tang N,Sadigh Y,Baigent S,Shen Z,Nair V,Yao Y

    更新日期：2018-05-24 00:00:00

  • Whole-Genome Sequencing of Human Enteroviruses from Clinical Samples by Nanopore Direct RNA Sequencing.

    abstract：:Enteroviruses are small RNA viruses that affect millions of people each year by causing an important burden of disease with a broad spectrum of symptoms. In routine diagnostic laboratories, enteroviruses are identified by PCR-based methods, often combined with partial sequencing for genotyping. In this proof-of-princi...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v12080841

    authors： Grädel C,Terrazos Miani MA,Baumann C,Barbani MT,Neuenschwander S,Leib SL,Suter-Riniker F,Ramette A

    更新日期：2020-07-31 00:00:00

  • Vaccinia Virus Natural Infections in Brazil: The Good, the Bad, and the Ugly.

    abstract：:The orthopoxviruses (OPV) comprise several emerging viruses with great importance to human and veterinary medicine, including vaccinia virus (VACV), which causes outbreaks of bovine vaccinia (BV) in South America. Historically, VACV is the most comprehensively studied virus, however, its origin and natural hosts remai...

    journal_title：Viruses

    pub_type： 杂志文章,评审

    doi：10.3390/v9110340

    authors： Oliveira JS,Figueiredo PO,Costa GB,Assis FL,Drumond BP,da Fonseca FG,Nogueira ML,Kroon EG,Trindade GS

    更新日期：2017-11-15 00:00:00

  • Distinct Molecular Mechanisms of Host Immune Response Modulation by Arenavirus NP and Z Proteins.

    abstract：:Endemic to West Africa and South America, mammalian arenaviruses can cross the species barrier from their natural rodent hosts to humans, resulting in illnesses ranging from mild flu-like syndromes to severe and fatal haemorrhagic zoonoses. The increased frequency of outbreaks and associated high fatality rates of the...

    journal_title：Viruses

    pub_type： 杂志文章,评审

    doi：10.3390/v12070784

    authors： Stott RJ,Strecker T,Foster TL

    更新日期：2020-07-21 00:00:00

  • Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays.

    abstract：:SARS-CoV-2 enters cells using its Spike protein, which is also the main target of neutralizing antibodies. Therefore, assays to measure how antibodies and sera affect Spike-mediated viral infection are important for studying immunity. Because SARS-CoV-2 is a biosafety-level-3 virus, one way to simplify such assays is ...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v12050513

    authors： Crawford KHD,Eguia R,Dingens AS,Loes AN,Malone KD,Wolf CR,Chu HY,Tortorici MA,Veesler D,Murphy M,Pettie D,King NP,Balazs AB,Bloom JD

    更新日期：2020-05-06 00:00:00

  • Sphingolipids as Potential Therapeutic Targets against Enveloped Human RNA Viruses.

    abstract：:Several notable human diseases are caused by enveloped RNA viruses: influenza, AIDS, hepatitis C, dengue hemorrhagic fever, microcephaly, and Guillain-Barré Syndrome. Being enveloped, the life cycle of this group of viruses is critically dependent on host lipid biosynthesis. Viral binding and entry involve interaction...

    journal_title：Viruses

    pub_type： 杂志文章,评审

    doi：10.3390/v11100912

    authors： Yager EJ,Konan KV

    更新日期：2019-10-01 00:00:00

  • In Vitro and In Vivo Antiviral Activity of Gingerenone A on Influenza A Virus Is Mediated by Targeting Janus Kinase 2.

    abstract：:Janus kinase (JAK) inhibitors have been developed as novel immunomodulatory drugs and primarily used for treating rheumatoid arthritis and other inflammatory diseases. Recent studies have suggested that this category of anti-inflammatory drugs could be potentially useful for the control of inflammation "storms" in res...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v12101141

    authors： Wang J,Prinz RA,Liu X,Xu X

    更新日期：2020-10-08 00:00:00

  • Caerin1.1 Suppresses the Growth of Porcine Epidemic Diarrhea Virus In Vitro via Direct Binding to the Virus.

    abstract：:Porcine epidemic diarrhea (PED) has re-emerged in recent years and has already caused huge economic losses to the porcine industry all over the world. Therefore, it is urgent for us to find out efficient ways to prevent and control this disease. In this study, the antiviral activity of a cationic amphibian antimicrobi...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v10090507

    authors： Guo N,Zhang B,Hu H,Ye S,Chen F,Li Z,Chen P,Wang C,He Q

    更新日期：2018-09-18 00:00:00

  • Expression Level of Small Envelope Protein in Addition to Sequence Divergence inside Its Major Hydrophilic Region Contributes to More Efficient Surface Antigen Secretion by Hepatitis B Virus Subgenotype D2 than Subgenotype A2.

    abstract：:Hepatitis B surface antigen (HBsAg) promotes persistent hepatitis B virus (HBV) infection. It primarily corresponds to small (S) envelope protein secreted as subviral particles. We previously found that genotype D clones expressed less S protein than genotype A clones but showed higher extracellular/intracellular rati...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v12090967

    authors： Wang Q,Fu S,Zhang J,Yuan Q,Li J,Xia N,Wen YM,Wang Y,Tong S

    更新日期：2020-09-01 00:00:00

  • Engineering RNA Virus Interference via the CRISPR/Cas13 Machinery in Arabidopsis.

    abstract：:Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) systems are key immune mechanisms helping prokaryotic species fend off RNA and DNA viruses. CRISPR/Cas9 has broad applications in basic research and biotechnology and has been widely used across eukaryotic species for genome...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v10120732

    authors： Aman R,Mahas A,Butt H,Aljedaani F,Mahfouz M

    更新日期：2018-12-19 00:00:00

  • Suppression of a Subset of Interferon-Induced Genes by Human Papillomavirus Type 16 E7 via a Cyclin Dependent Kinase 8-Dependent Mechanism.

    abstract：:Persistent infection by human papillomaviruses (HPVs), small, double-stranded DNA viruses that infect keratinocytes of the squamous epithelia, can lead to the development of cervical and other cancers. The viral oncoprotein E7 contributes to viral persistence in part by regulating host gene expression through binding ...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v12030311

    authors： Rice S,Kim SM,Rodriguez C,Songock W,Raikhy G,Lopez R,Henderson L,Yusufji A,Bodily J

    更新日期：2020-03-13 00:00:00

  • Modes of Human T Cell Leukemia Virus Type 1 Transmission, Replication and Persistence.

    abstract：:Human T-cell leukemia virus type 1 (HTLV-1) is a retrovirus that causes cancer (Adult T cell Leukemia, ATL) and a spectrum of inflammatory diseases (mainly HTLV-associated myelopathy-tropical spastic paraparesis, HAM/TSP). Since virions are particularly unstable, HTLV-1 transmission primarily occurs by transfer of a c...

    journal_title：Viruses

    pub_type： 杂志文章,评审

    doi：10.3390/v7072793

    authors： Carpentier A,Barez PY,Hamaidia M,Gazon H,de Brogniez A,Perike S,Gillet N,Willems L

    更新日期：2015-07-07 00:00:00

  • Upregulation of miR-101 during Influenza A Virus Infection Abrogates Viral Life Cycle by Targeting mTOR Pathway.

    abstract：:Micro RNAs (miRNAs) are a class of small non-coding single-stranded RNA, which play an important role in modulating host-Influenza A virus (IAV) crosstalk. The interplay between influenza and miRNA interaction is defined by a plethora of complex mechanisms, which are not fully understood yet. Here, we demonstrate that...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v12040444

    authors： Sharma S,Chatterjee A,Kumar P,Lal S,Kondabagil K

    更新日期：2020-04-15 00:00:00

  • Merkel Cell Polyomavirus Large T Antigen Unique Domain Regulates Its Own Protein Stability and Cell Growth.

    abstract：:Merkel cell polyomavirus (MCV) is the only known human oncogenic virus in the polyomaviridae family and the etiological agent of most Merkel cell carcinomas (MCC). MCC is an aggressive and highly metastatic skin cancer with a propensity for recurrence and poor prognosis. Large tumor antigen (LT), is an essential oncop...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v12091043

    authors： Nwogu N,Ortiz LE,Kwun HJ

    更新日期：2020-09-18 00:00:00

  • Peroxisome Proliferator-Activated Receptor Gamma (PPAR) Suppresses Inflammation and Bacterial Clearance during Influenza-Bacterial Super-Infection.

    abstract：:Influenza virus is among the most common causes of respiratory illness worldwide and can be complicated by secondary bacterial pneumonia, a frequent cause of mortality. When influenza virus infects the lung, the innate immune response is activated, and interferons and inflammatory mediators are released. This "cytokin...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v11060505

    authors： Gopal R,Mendy A,Marinelli MA,Richwalls LJ,Seger PJ,Patel S,McHugh KJ,Rich HE,Grousd JA,Forno E,Alcorn JF

    更新日期：2019-06-01 00:00:00

  • Oropouche Fever: A Review.

    abstract：:Oropouche fever is an emerging zoonotic disease caused by Oropouche virus (OROV), an arthropod transmitted Orthobunyavirus circulating in South and Central America. During the last 60 years, more than 30 epidemics and over half a million clinical cases attributed to OROV infection have been reported in Brazil, Peru, P...

    journal_title：Viruses

    pub_type： 杂志文章,评审

    doi：10.3390/v10040175

    authors： Sakkas H,Bozidis P,Franks A,Papadopoulou C

    更新日期：2018-04-04 00:00:00

  • Noncanonical expression of a murine cytomegalovirus early protein CD8 T-cell epitope as an immediate early epitope based on transcription from an upstream gene.

    abstract：:Viral CD8 T-cell epitopes, represented by viral peptides bound to major histocompatibility complex class-I (MHC-I) glycoproteins, are often identified by "reverse immunology", a strategy not requiring biochemical and structural knowledge of the actual viral protein from which they are derived by antigen processing. In...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v6020808

    authors： Fink A,Büttner JK,Thomas D,Holtappels R,Reddehase MJ,Lemmermann NA

    更新日期：2014-02-14 00:00:00

  • Pectobacterium atrosepticum Phage vB_PatP_CB5: A Member of the Proposed Genus 'Phimunavirus'.

    abstract：:Pectobacterium atrosepticum is a phytopathogen of economic importance as it is the causative agent of potato blackleg and soft rot. Here we describe the Pectobacterium phage vB_PatP_CB5 (abbreviated as CB5), which specifically infects the bacterium. The bacteriophage is characterized in detail and TEM micrographs indi...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v10080394

    authors： Buttimer C,Lucid A,Neve H,Franz CMAP,O'Mahony J,Turner D,Lavigne R,Coffey A

    更新日期：2018-07-26 00:00:00

  • Live Attenuated African Swine Fever Viruses as Ideal Tools to Dissect the Mechanisms Involved in Cross-Protection.

    abstract：:African swine fever (ASF) has become the major threat for the global swine industry. Furthermore, the epidemiological situation of African swine fever virus (ASFV) in some endemic regions of Sub-Saharan Africa is worse than ever, with multiple virus strains and genotypes currently circulating in a given area. Despite ...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v12121474

    authors： Lopez E,van Heerden J,Bosch-Camós L,Accensi F,Navas MJ,López-Monteagudo P,Argilaguet J,Gallardo C,Pina-Pedrero S,Salas ML,Salt J,Rodriguez F

    更新日期：2020-12-21 00:00:00

  • Hazard Characterization of Modified Vaccinia Virus Ankara Vector: What Are the Knowledge Gaps?

    abstract：:Modified vaccinia virus Ankara (MVA) is the vector of choice for human and veterinary applications due to its strong safety profile and immunogenicity in vivo. The use of MVA and MVA-vectored vaccines against human and animal diseases must comply with regulatory requirements as they pertain to environmental risk asses...

    journal_title：Viruses

    pub_type： 杂志文章,评审

    doi：10.3390/v9110318

    authors： Okeke MI,Okoli AS,Diaz D,Offor C,Oludotun TG,Tryland M,Bøhn T,Moens U

    更新日期：2017-10-29 00:00:00

  • Conflict in the Intracellular Lives of Endosymbionts and Viruses: A Mechanistic Look at Wolbachia-Mediated Pathogen-blocking.

    abstract：:At the forefront of vector control efforts are strategies that leverage host-microbe associations to reduce vectorial capacity. The most promising of these efforts employs Wolbachia, a maternally transmitted endosymbiotic bacterium naturally found in 40% of insects. Wolbachia can spread through a population of insects...

    journal_title：Viruses

    pub_type： 杂志文章,评审

    doi：10.3390/v10040141

    authors： Lindsey ARI,Bhattacharya T,Newton ILG,Hardy RW

    更新日期：2018-03-21 00:00:00

  • Base composition and translational selection are insufficient to explain codon usage bias in plant viruses.

    abstract：:Viral codon usage bias may be the product of a number of synergistic or antagonistic factors, including genomic nucleotide composition, translational selection, genomic architecture, and mutational or repair biases. Most studies of viral codon bias evaluate only the relative importance of genomic base composition and ...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v5010162

    authors： Cardinale DJ,DeRosa K,Duffy S

    更新日期：2013-01-15 00:00:00

  • Autophagy and Noroviruses.

    abstract：:Autophagy is an essential cellular process by which a cell degrades materials within its cytoplasm. Intracellular pathogens like viruses must deal with autophagy, either positively or negatively, for their own survival and replication. For some viruses, autophagy can even play proviral roles, helping their replication...

    journal_title：Viruses

    pub_type： 杂志文章,评审

    doi：10.3390/v11030244

    authors： Furlong K,Hwang S

    更新日期：2019-03-12 00:00:00

  • Enterovirus Transmission by Secretory Autophagy.

    abstract：:Present in many cell types, non-degradative secretory autophagy is a newly discovered pathway in which autophagosomes fuse with the plasma membrane instead of lysosomes. Surprisingly, some viruses exploit secretory autophagy to exit cells non-lytically, shedding into the extracellular environment as particle populatio...

    journal_title：Viruses

    pub_type： 杂志文章,评审

    doi：10.3390/v10030139

    authors： Mutsafi Y,Altan-Bonnet N

    更新日期：2018-03-20 00:00:00

  • The Zebrafish Xenograft Platform-A Novel Tool for Modeling KSHV-Associated Diseases.

    abstract：:Kaposi's sarcoma associated-herpesvirus (KSHV, also known as human herpesvirus-8) is a gammaherpesvirus that establishes life-long infection in human B lymphocytes. KSHV infection is typically asymptomatic, but immunosuppression can predispose KSHV-infected individuals to primary effusion lymphoma (PEL); a malignancy ...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v12010012

    authors： Pringle ES,Wertman J,Melong N,Coombs AJ,Young AL,O'Leary D,Veinotte C,Robinson CA,Ha MN,Dellaire G,Druley TE,McCormick C,Berman JN

    更新日期：2019-12-20 00:00:00

  • Novel Flavivirus Attenuation Markers Identified in the Envelope Protein of Alfuy Virus.

    abstract：:Alfuy (ALFV) is an attenuated flavivirus related to the Murray Valley encephalitis virus (MVEV). We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273-277) and lack of glycosylation at E154-156. To further determine the mechanis...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v13020147

    authors： Westlake D,Bielefeldt-Ohmann H,Prow NAA,Hall RAA

    更新日期：2021-01-20 00:00:00

  • The HIV-1 Reverse Transcriptase A62V Mutation Influences Replication Fidelity and Viral Fitness in the Context of Multi-Drug-Resistant Mutations.

    abstract：:Emergence of human immunodeficiency virus type 1 (HIV-1) drug resistance arises from mutation fixation in the viral genome during antiretroviral therapy. Primary mutations directly confer antiviral drug resistance, while secondary mutations arise that do not confer drug resistance. The A62V amino acid substitution in ...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v10070376

    authors： Maldonado JO,Mansky LM

    更新日期：2018-07-19 00:00:00

  • Phage Lytic Enzymes.

    abstract：:Phage lytic enzymes are enzymes produced by bacterial viruses, either as part of their virion to facilitate bacterial infection through local peptidoglycan degradation, or as soluble proteins to induce massive cell lysis at the end of the lytic replication cycle [...]. ...

    journal_title：Viruses

    pub_type： 社论

    doi：10.3390/v11020113

    authors： Briers Y

    更新日期：2019-01-29 00:00:00

  • Porcine HMGCR Inhibits Porcine Circovirus Type 2 Infection by Directly Interacting with the Viral Proteins.

    abstract：:Porcine circovirus type 2 (PCV2) is the etiological agent of porcine circovirus diseases and porcine circovirus-associated diseases (PCVDs/PCVADs). However, the pathogenesis of PCV2 is not fully understood. We previously found that 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) is negatively associated with P...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v11060544

    authors： Ouyang T,Niu G,Zhang Y,Liu X,Zhang X,Zhang S,Geng Y,Pang D,Ouyang H,Ren L

    更新日期：2019-06-11 00:00:00

  • Orchestrating the Selection and Packaging of Genomic RNA by Retroviruses: An Ensemble of Viral and Host Factors.

    abstract：:Infectious retrovirus particles contain two copies of unspliced viral RNA that serve as the viral genome. Unspliced retroviral RNA is transcribed in the nucleus by the host RNA polymerase II and has three potential fates: (1) it can be spliced into subgenomic messenger RNAs (mRNAs) for the translation of viral protein...

    journal_title：Viruses

    pub_type： 杂志文章,评审

    doi：10.3390/v8090257

    authors： Kaddis Maldonado RJ,Parent LJ

    更新日期：2016-09-20 00:00:00