Abstract:
BACKGROUND:The development of cellular therapies to treat muscle wastage with disease or age is paramount. Resident muscle satellite cells are not currently regarded as a viable cell source due to their limited migration and growth capability ex vivo. This study investigated the potential of muscle-derived PW1+/Pax7- interstitial progenitor cells (PICs) as a source of tissue-specific stem/progenitor cells with stem cell properties and multipotency. METHODS:Sca-1+/PW1+ PICs were identified on tissue sections from hind limb muscle of 21-day-old mice, isolated by magnetic-activated cell sorting (MACS) technology and their phenotype and characteristics assessed over time in culture. Green fluorescent protein (GFP)-labelled PICs were used to determine multipotency in vivo in a tumour formation assay. RESULTS:Isolated PICs expressed markers of pluripotency (Oct3/4, Sox2, and Nanog), were clonogenic, and self-renewing with >60 population doublings, and a population doubling time of 15.8 ± 2.9 h. PICs demonstrated an ability to generate both striated and smooth muscle, whilst also displaying the potential to differentiate into cell types of the three germ layers both in vitro and in vivo. Moreover, PICs did not form tumours in vivo. CONCLUSION:These findings open new avenues for a variety of solid tissue engineering and regeneration approaches, utilising a single multipotent stem cell type isolated from an easily accessible source such as skeletal muscle.
journal_name
Stem Cell Res Therjournal_title
Stem cell research & therapyauthors
Cottle BJ,Lewis FC,Shone V,Ellison-Hughes GMdoi
10.1186/s13287-017-0612-4subject
Has Abstractpub_date
2017-07-04 00:00:00pages
158issue
1issn
1757-6512pii
10.1186/s13287-017-0612-4journal_volume
8pub_type
杂志文章abstract::Evidence suggests that transdifferentiation of mesenchymal stem cells (MSCs) into various neuronal cells contributes to functional recovery after experimental spinal cord injury. Qiu et al. have recently published an exciting article in Stem Cell Research & Therapy demonstrating the transdifferentiation of already dif...
journal_title:Stem cell research & therapy
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doi:10.1186/s13287-015-0186-y
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journal_title:Stem cell research & therapy
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doi:10.1186/s13287-015-0044-y
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journal_title:Stem cell research & therapy
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abstract::Ischemic heart disease is the major cause of death globally, and a recently developed stem cell transplantation is a promising therapy for myocardial infarction. Mesenchymal stem cells (MSCs) exist in a wide range of tissues, and their differentiation potential and immunoregulatory capacity make them a more optimal ca...
journal_title:Stem cell research & therapy
pub_type: 评论,杂志文章
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journal_title:Stem cell research & therapy
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journal_title:Stem cell research & therapy
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journal_title:Stem cell research & therapy
pub_type: 杂志文章
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abstract::Owing to the aging of the population, our society now faces an impending wave of age-related neurodegenerative pathologies, the most significant of which is Alzheimer's disease. Currently, no effective therapies for Alzheimer's disease have been developed. However, recent advances in the fields of neural stem cells an...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
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journal_title:Stem cell research & therapy
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journal_title:Stem cell research & therapy
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更新日期:2020-08-27 00:00:00
abstract:BACKGROUND:Exploring the effects of lncRNA SNHG1 in the process of osteogenic differentiation of periodontal ligament stem cells (PDLSCs) would provide novel therapeutic strategies for tissue regeneration. METHODS:Loss-of-function and gain-of-function assays were induced by lentivirus. The osteogenic differentiation o...
journal_title:Stem cell research & therapy
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journal_title:Stem cell research & therapy
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doi:10.1186/s13287-015-0098-x
更新日期:2015-05-22 00:00:00
abstract:BACKGROUND:Bone marrow mesenchymal stem cells (BMSCs) exhibit the capacity to self-renew and differentiate into multi-lineage cell types, including osteoblasts, which are crucial regulators of fracture healing. Thus, this study aims to investigate the effect of microRNA (miR)-22-3p from BMSC-derived EVs on osteogenic d...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-020-01707-6
更新日期:2020-06-10 00:00:00
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journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-015-0204-0
更新日期:2015-10-31 00:00:00
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journal_title:Stem cell research & therapy
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journal_title:Stem cell research & therapy
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journal_title:Stem cell research & therapy
pub_type: 杂志文章,评审
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journal_title:Stem cell research & therapy
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更新日期:2019-01-03 00:00:00
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journal_title:Stem cell research & therapy
pub_type: 杂志文章,评审
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更新日期:2018-04-13 00:00:00
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journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-015-0016-2
更新日期:2015-03-13 00:00:00
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journal_title:Stem cell research & therapy
pub_type: 杂志文章
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更新日期:2013-06-04 00:00:00
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journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-017-0532-3
更新日期:2017-04-18 00:00:00
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journal_title:Stem cell research & therapy
pub_type: 杂志文章
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更新日期:2020-04-06 00:00:00
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journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-018-1084-x
更新日期:2018-12-07 00:00:00
abstract:BACKGROUND:Neonatal hypoxia-ischemia induces massive brain damage during the perinatal period, resulting in long-term consequences to central nervous system structural and functional maturation. Although neural progenitor cells (NPCs) migrate through the parenchyma and home in to injury sites in the rodent brain, the m...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-017-0474-9
更新日期:2017-02-07 00:00:00