Abstract:
INTRODUCTION:Endothelial dysfunction is found in different pathologies such as diabetes and renal and heart diseases, representing one of the major health problems. The reduced vasodilation of impaired endothelium starts a prothrombotic state associated with irregular blood flow. We aimed to explore the potential of amniotic fluid stem (AFS) cells as a source for regenerative medicine in this field; for the first time, we focused on third-trimester amniotic fluid AFS cells and compared them with the already-described AFS cells from the second trimester. METHODS:Cells from the two trimesters were cultured, selected and expanded in normoxia (20 % oxygen) and hypoxia (5 % oxygen). Cells were analysed to compare markers, proliferation rate and differentiation abilities. Endothelial potential was assessed not only in vitro-Matrigel tube formation assay, acetylated human low-density lipoprotein (AcLDL) uptake-but also in vivo (Matrigel plug with cell injection and two animal models). Specifically, for the latter, we used established protocols to assess the involvement of AFS cells in two different mouse models of endothelial dysfunction: (1) a chronic ischemia model with local injection of cells and (2) an electric carotid damage where cells were systemically injected. RESULTS:We isolated and expanded AFS cells from third-trimester amniotic fluid samples by using CD117 as a selection marker. Hypoxia enhanced the proliferation rate, the surface protein pattern was conserved between the trimesters and comparable differentiation was achieved after culture in both normoxia and hypoxia. Notably, the expression of early endothelial transcription factors and AngiomiRs was detected before and after induction. When in vivo, AFS cells from both trimesters expanded in hypoxia were able to rescue the surface blood flow when locally injected in mice after chronic ischemia damage, and importantly AFS cells at term of gestation possessed enhanced ability to fix carotid artery electric damage compared with AFS cells from the second trimester. CONCLUSIONS:To the best of our knowledge, this is the first research work that fully characterizes AFS cells from the third trimester for regenerative medicine purposes. The results highlight how AFS cells, in particular at term of gestation and cultured in hypoxia, can be considered a promising source of stem cells possessing significant endothelial regenerative potential.
journal_name
Stem Cell Res Therjournal_title
Stem cell research & therapyauthors
Schiavo AA,Franzin C,Albiero M,Piccoli M,Spiro G,Bertin E,Urbani L,Visentin S,Cosmi E,Fadini GP,De Coppi P,Pozzobon Mdoi
10.1186/s13287-015-0204-0subject
Has Abstractpub_date
2015-10-31 00:00:00pages
209issn
1757-6512pii
10.1186/s13287-015-0204-0journal_volume
6pub_type
杂志文章abstract:BACKGROUND:Neural stem cells for the treatment of spinal cord injury (SCI) are of particular interest for future therapeutic use. However, until now, stem cell therapies are often limited due to the inhibitory environment following the injury. Therefore, in this study, we aimed at testing a combinatorial approach with ...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-015-0268-x
更新日期:2016-01-13 00:00:00
abstract:BACKGROUND:Mesenchymal stem cells (MSCs) were shown to harbor therapeutic potential in models of respiratory diseases, such as bronchopulmonary dysplasia (BPD), the most common sequel of preterm birth. In these studies, cells or animals were challenged with hyperoxia or other injury-inducing agents. However, little is ...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-020-02028-4
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abstract:BACKGROUND:Liver organoid technology holds great promises to be used in large-scale population-based drug screening and in future regenerative medicine strategies. Recently, some studies reported robust protocols for generating isogenic liver organoids using liver parenchymal and non-parenchymal cells derived from indu...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-019-1367-x
更新日期:2019-08-15 00:00:00
abstract:BACKGROUND:Sepsis is a global inflammatory disease that causes death. It has been reported that mesenchymal stem cell (MSC) treatment can attenuate inflammatory and septic symptoms. In this study, we investigated how interactions between neutrophils and human umbilical cord blood (hUCB)-MSCs in the liver of septic mice...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-020-1559-4
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abstract:BACKGROUND:Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) from the human umbilical cord have been studied extensively due to their immunomodulatory functions. Large-conductance Ca2+-activated K+ (BKCa channels) channels are involved in many inflammatory responses, but their involvement in the anti-inflammator...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-020-01952-9
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abstract:BACKGROUND:Mesenchymal stromal cell (MSC)-based therapy is a promising strategy for preventing the progression of chronic kidney disease (CKD), with the potential to induce tissue regeneration. In search of the best cellular source we compared, in the rat model of adriamycin (ADR) nephropathy, the regenerative potentia...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-018-0960-8
更新日期:2018-08-14 00:00:00
abstract::Ischemic heart disease is the major cause of death globally, and a recently developed stem cell transplantation is a promising therapy for myocardial infarction. Mesenchymal stem cells (MSCs) exist in a wide range of tissues, and their differentiation potential and immunoregulatory capacity make them a more optimal ca...
journal_title:Stem cell research & therapy
pub_type: 评论,杂志文章
doi:10.1186/scrt213
更新日期:2013-06-05 00:00:00
abstract:INTRODUCTION:Cell-based therapy represents a new frontier in the treatment of a wide variety of human diseases traditionally associated with morbidity outcomes, including those involving inflammation, autoimmunity, tissue damage, and cancer. However, the use of mesenchymal stem cells (MSCs) to treat multiple myeloma (M...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/scrt322
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:The transfection of human mesenchymal stem cells (hMSCs) with the hyperpolarization-activated cyclic nucleotide-gated ion channel 2 (HCN2) gene has been demonstrated to provide biological pacing in dogs with complete heart block. The mechanism appears to be the generation of the ion current (If) by the HCN2-...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-016-0326-z
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journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-015-0021-5
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abstract:INTRODUCTION:Degenerative retinal diseases like age-related macular degeneration (AMD) are the leading cause of blindness. Cell transplantation showed promising therapeutic effect for such diseases, and embryonic stem cell (ESC) is one of the sources of such donor cells. Here, we aimed to generate retinal progenitor ce...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-015-0207-x
更新日期:2015-11-09 00:00:00
abstract::Extracellular vesicles (EVs) contain proteins, microRNAs, mRNAs, long non-coding RNAs, and phospholipids, and are a novel mechanism of intercellular communication. It has been proposed that the immunomodulatory and regenerative effects of mesenchymal stem/stromal cells (MSCs) are mainly mediated by soluble paracrine f...
journal_title:Stem cell research & therapy
pub_type: 杂志文章,评审
doi:10.1186/s13287-019-1484-6
更新日期:2019-11-28 00:00:00
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journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-018-0956-4
更新日期:2018-08-09 00:00:00
abstract:INTRODUCTION:Studies have shown that stem cells exert their therapeutic effects on acute kidney injury (AKI) through paracrine/endocrine actions. If the protective effect is mediated in an endocrine manner, the injection of the factors that these cells secrete could be effective, but the effect of conditioned medium (C...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/scrt489
更新日期:2014-08-21 00:00:00
abstract::The use of secretome may be a new strand of cell therapy, which is equal to or even superior to the injection of live cells, called cell-free therapy. In ovarian transplantation, this approach may be a therapeutic possibility for the ovarian graft in hypoxia. We designed the present study to evaluate whether the cell-...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-018-1054-3
更新日期:2018-11-21 00:00:00
abstract:BACKGROUND:Recent studies have demonstrated mesenchymal stem cells (MSCs) as effective mitochondrial donors with therapeutic success in multiple experimental models of human disease. MSCs obtained from different tissue sources such as bone marrow (BM), adipose (AD), dental pulp (DP), and Wharton's jelly (WJ) are routin...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-018-1012-0
更新日期:2018-11-08 00:00:00
abstract:BACKGROUND:Nitric oxide (NO) plays a role in a number of physiological processes including stem cell differentiation and osteogenesis. Endothelial nitric oxide synthase (eNOS), one of three NO-producing enzymes, is located in a close conformation with the caveolin-1 (CAV-1WT) membrane protein which is inhibitory to NO ...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-016-0442-9
更新日期:2016-12-07 00:00:00
abstract:BACKGROUND:Exploring the effects of lncRNA SNHG1 in the process of osteogenic differentiation of periodontal ligament stem cells (PDLSCs) would provide novel therapeutic strategies for tissue regeneration. METHODS:Loss-of-function and gain-of-function assays were induced by lentivirus. The osteogenic differentiation o...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-020-01953-8
更新日期:2020-10-07 00:00:00
abstract:BACKGROUND:Autologous epidermal basal cell suspension therapy has been proven to be one of the most effective treatments for full-thickness wounds. However, we found there remain obvious defects that significantly confined the utilization and function of the epidermal basal cells (EBCs), especially the epidermal stem c...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-019-1236-7
更新日期:2019-09-11 00:00:00
abstract::Periostin is a matricellular protein that is reactivated during tissue damage and repair and has been shown to be a critical regulator of multiple biological pathways involved in the repair of tissue after myocardial infarction, peripheral vascular disease, and skin wounds. The tissue repair properties attributed to p...
journal_title:Stem cell research & therapy
pub_type: 评论,杂志文章
doi:10.1186/s13287-015-0215-x
更新日期:2015-11-04 00:00:00
abstract:BACKGROUND:Efficient and sustained hematopoietic recovery after hematopoietic stem cell or bone marrow transplantation is supported by paracrine signaling from specific subpopulations of mesenchymal stromal cells (MSCs). Here, we considered whether in vitro mechanopriming of human MSCs could be administered to predicti...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-018-0982-2
更新日期:2018-10-24 00:00:00
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journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/scrt151
更新日期:2013-01-06 00:00:00
abstract:BACKGROUND:Graft-versus-host disease (GvHD) is the main life-threatening complication of allogeneic hematopoietic stem cell transplantation (HSCT). Thirty to 80% of GvHD patients do not respond to first-line treatment and a second-line treatment is not universally established. Based on their immunomodulatory properties...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-020-01592-z
更新日期:2020-02-18 00:00:00
abstract:BACKGROUND:The development of cellular therapies to treat muscle wastage with disease or age is paramount. Resident muscle satellite cells are not currently regarded as a viable cell source due to their limited migration and growth capability ex vivo. This study investigated the potential of muscle-derived PW1+/Pax7- i...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-017-0612-4
更新日期:2017-07-04 00:00:00
abstract:INTRODUCTION:Stem cells have been evaluated as a potential therapeutic approach for several neurological disorders of the central and peripheral nervous system as well as for traumatic brain and spinal cord injury. Currently, the lack of a reliable and safe method to accurately and non-invasively locate the site of imp...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-015-0129-7
更新日期:2015-07-21 00:00:00
abstract::The outbreak of 2019 novel coronavirus disease (COVID-19) worldwide is becoming rapidly a major concern. The number of severe cases has increased dramatically worldwide, while specific treatment options are scarce. The main pathologic features of severe or critical COVID-19 were consistent with acute lung injure (ALI)...
journal_title:Stem cell research & therapy
pub_type: 信件
doi:10.1186/s13287-020-01678-8
更新日期:2020-05-04 00:00:00
abstract:INTRODUCTION:A feature which makes stem cells promising candidates for cell therapy is their ability to migrate effectively into damaged or diseased tissues. Recent reports demonstrated the increased motility of human mesenchymal stem cells (hMSC) grown under hypoxic conditions compared to normoxic cells. However, the ...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/scrt153
更新日期:2013-01-07 00:00:00
abstract:BACKGROUND:Emerging evidence suggests that neural crest-derived cells (NCCs) present important functions in peripheral nerve regeneration to correct the insufficiency of autogenous Schwann cells. Postmigratory NCCs have been successfully isolated from adult rat bone marrow in our previous work. In this study, we aim to...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-019-1517-1
更新日期:2019-12-18 00:00:00
abstract:OBJECTIVE:Autograft microskin transplantation has been widely used as a skin graft therapy in full-thickness skin defect. However, skin grafting failure can lead to a pathological delay wound healing due to a poor vascularization bed. Considering the active role of adipose-derived stem cell (ADSC) in promoting angiogen...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-019-1389-4
更新日期:2019-08-30 00:00:00
abstract:BACKGROUND:For adipose-derived stromal cells (ASCs) to be safe for use in the clinical setting, they need to be prepared using good manufacturing practices (GMPs). Fetal bovine serum (FBS), used to expand ASCs in vitro in some human clinical trials, runs the risk of xenoimmunization and zoonotic disease transmission. T...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-019-1370-2
更新日期:2019-08-14 00:00:00