Abstract：
OBJECTIVE:Autograft microskin transplantation has been widely used as a skin graft therapy in full-thickness skin defect. However, skin grafting failure can lead to a pathological delay wound healing due to a poor vascularization bed. Considering the active role of adipose-derived stem cell (ADSC) in promoting angiogenesis, we intend to investigate the efficacy of autograft microskin combined with ADSC transplantation for facilitating wound healing in a full-thickness skin defect mouse model. MATERIAL AND METHODS:An in vivo full-thickness skin defect mouse model was used to evaluate the contribution of transplantation microskin and ADSC in wound healing. The angiogenesis was detected by immunohistochemistry staining. In vitro paracrine signaling pathway was evaluated by protein array and Gene Ontology, Kyoto Encyclopedia of Genes and Genomes pathway, and protein-protein interaction network analysis. RESULTS:Co-transplantation of microskin and ADSC potentiated the wound healing with better epithelization, smaller scar thickness, and higher angiogenesis (CD31) in the subcutaneous layer. We found both EGF and VEGF cytokines were secreted by microskin in vitro. Additionally, secretome proteomic analysis in a co-culture system of microskin and ADSC revealed that ADSC could secrete a wide range of important molecules to form a reacting network with microskin, including VEGF, IL-6, EGF, uPAR, MCP-3, G-CSF, and Tie-2, which most likely supported the angiogenesis effect as observed. CONCLUSION:Overall, we concluded that the use of ADSC partially modulates microskin function and enhances wound healing by promoting angiogenesis in a full-thickness skin defect mouse model.
journal_name
Stem Cell Res Therjournal_title
Stem cell research & therapyauthors
Luo Y,Yi X,Liang T,Jiang S,He R,Hu Y,Bai L,Wang C,Wang K,Zhu Ldoi
10.1186/s13287-019-1389-4subject
Has Abstractpub_date
2019-08-30 00:00:00pages
279issue
1issn
1757-6512pii
10.1186/s13287-019-1389-4journal_volume
10pub_type
杂志文章abstract：BACKGROUND:Vocal fold (VF) scarring, caused by surgery or inflammation, often results in severe voice problems or aphonia. Effective lasting treatment is lacking. Previous in vitro and in vivo animal studies reported positive effects on VF scar resolution with mesenchymal stromal cell (MSC) implantation. The principal ...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-01632-8
更新日期：2020-03-20 00:00:00
abstract：INTRODUCTION:Despite attempts to prevent brain injury during the hyperacute phase of stroke, most sufferers end up with significant neuronal loss and functional deficits. The use of cell-based therapies to recover the injured brain offers new hope. In the current study, we employed human neural stem cells (hNSCs) isola...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-015-0175-1
更新日期：2015-09-29 00:00:00
abstract：INTRODUCTION:Mesenchymal stromal cells (MSCs) play an important role in the prevention of cell and tissue fibrosis. Senescence may decrease the function of MSCs during recovery from tissue and organ damage. Extracellular vesicles (EVs) released from MSCs contribute to the repair of kidney injury. We explored the influe...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-01792-7
更新日期：2020-07-08 00:00:00
abstract：BACKGROUND:Mesenchymal stromal cells (MSCs) have been largely investigated, in the past decade, as potential therapeutic strategies for various acute and chronic pathological conditions. MSCs isolated from different sources, such as bone marrow (BM), umbilical cord tissue (UCT) and adipose tissue (AT), share many biolo...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-016-0383-3
更新日期：2016-08-25 00:00:00
abstract：BACKGROUND:Many studies have shown that long noncoding RNAs (lncRNAs) are closely related to the stimulation of osteogenic differentiation of adipose-derived stem cells (ADSCs) and the prevention of osteoporosis. Current research aimed to investigate the novel lncRNA and explored the function and molecular mechanism of...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-01754-z
更新日期：2020-06-17 00:00:00
abstract：BACKGROUND:iRoot BP Plus is a novel bioceramic endodontic material. Recently, it has been considered as an alternative to MTA which is the most popular scaffold cover during regenerative endodontic therapy. This study aimed to evaluate the effects of iRoot BP Plus on the osteo/odontogenic capacity of bone marrow mesenc...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-019-1345-3
更新日期：2019-07-29 00:00:00
abstract：BACKGROUND:Epidermal grafting using cells derived from pluripotent stem cells will change the face of this side of regenerative cutaneous medicine. To date, the safety of the graft would be the major unmet deal in order to implement long-term skin grafting. In this context, experiments on large animals appear unavoidab...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-017-0741-9
更新日期：2017-12-19 00:00:00
abstract：INTRODUCTION:Administration of bone marrow-derived cells produces beneficial effects in experimental extrapulmonary acute respiratory distress syndrome (ARDS). However, there are controversies regarding the effects of timing of cell administration and initial insult severity on inflammatory response. We evaluated the e...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/scrt334
更新日期：2013-10-13 00:00:00
abstract：AIM:Myocardial infarction (MI) is a severe disease with increased mortality and disability rates, posing heavy economic burden for society. Exosomes were uncovered to mediate intercellular communication after MI. This study aims to explore the effect and mechanism of lncRNA KLF3-AS1 in exosomes secreted by human mesenc...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-019-1522-4
更新日期：2019-12-17 00:00:00
abstract：INTRODUCTION:Studies have shown that stem cells exert their therapeutic effects on acute kidney injury (AKI) through paracrine/endocrine actions. If the protective effect is mediated in an endocrine manner, the injection of the factors that these cells secrete could be effective, but the effect of conditioned medium (C...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/scrt489
更新日期：2014-08-21 00:00:00
abstract：:Tissue engineering has developed many paradigms and techniques on how to best integrate cells and extracellular matrix to create in vitro structures that replicate native tissue. The strategy best suited for building these constructs depends mainly on the target cells, tissues, and organ of interest, and how readily t...
journal_title：Stem cell research & therapy
pub_type： 杂志文章,评审
doi：10.1186/scrt162
更新日期：2013-02-14 00:00:00
abstract：BACKGROUND:Bone marrow mesenchymal stem cells (BMSCs) exhibit the capacity to self-renew and differentiate into multi-lineage cell types, including osteoblasts, which are crucial regulators of fracture healing. Thus, this study aims to investigate the effect of microRNA (miR)-22-3p from BMSC-derived EVs on osteogenic d...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-01707-6
更新日期：2020-06-10 00:00:00
abstract：INTRODUCTION:Delivered systemically or natively circulating mesenchymal stem cells accumulate in injured tissues. During homing mesenchymal stem cells adhere to endothelial cells and infiltrate underlying tissue. Previously we have shown that adhesiveness of endothelial cells for mesenchymal stem cells correlates with ...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/scrt35
更新日期：2010-11-17 00:00:00
abstract：BACKGROUND:Mesenchymal stromal cells (MSCs) are considered to have a modest benefit on left ventricular ejection fraction (LVEF) in patients with acute myocardial infarction (AMI). However, the optimal injection timing and dose needed to induce beneficial cardiac effects are unknown. The purpose of this meta-analysis w...
journal_title：Stem cell research & therapy
pub_type： 杂志文章,meta分析
doi：10.1186/s13287-016-0450-9
更新日期：2017-01-28 00:00:00
abstract：BACKGROUND:Optimal cell type as cell-based therapies for heart failure (HF) remains unclear. We sought to compare the safety and efficacy of direct intramyocardial transplantation of human embryonic stem cell-derived cardiomyocytes (hESC-CMs) and human induced pluripotent stem cell-derived mesenchymal stem cells (hiPSC...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-019-1183-3
更新日期：2019-03-07 00:00:00
abstract：BACKGROUND:Spinal metastasis is a major challenge in patients with advanced lung cancer, but the mechanisms in the organotropism of metastasis are still unclear. Adipose-derived mesenchymal stem cells (ADSCs) exhibit cancer-promoting properties that influence the tumour microenvironment; however, there is no research o...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-019-1280-3
更新日期：2019-06-13 00:00:00
abstract：BACKGROUND:This study explored the details of the immune response after autologous hematopoietic stem cell transplantation (AHSCT) treatment in type 1 diabetes mellitus. METHODS:Peripheral blood mononuclear cells (PBMCs) from 18 patients with type 1 diabetes mellitus were taken at baseline and 12 months after AHSCT or...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-017-0542-1
更新日期：2017-04-18 00:00:00
abstract：:Mesenchymal stem cells have been used for cardiovascular regenerative therapy for decades. These cells have been established as one of the potential therapeutic agents, following several tests in animal models and clinical trials. In the process, various sources of mesenchymal stem cells have been identified which hel...
journal_title：Stem cell research & therapy
pub_type： 杂志文章,评审
doi：10.1186/s13287-016-0341-0
更新日期：2016-06-04 00:00:00
abstract：BACKGROUND:The Activin A and bone morphogenetic protein (BMP) pathways are critical regulators of the immune system and of bone formation. Inappropriate activation of these pathways, as in conditions of congenital heterotopic ossification, are thought to activate an osteogenic program in endothelial cells. However, if ...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-016-0372-6
更新日期：2016-08-17 00:00:00
abstract：INTRODUCTION:Topical administration of eye drops is the major route for drug delivery to the cornea. Orbital fat-derived stem cells (OFSCs) possess an in vitro corneal epithelial differentiation capacity. Both the safety and immunomodulatory ability of systemic OFSC transplantation were demonstrated in our previous wor...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/scrt223
更新日期：2013-06-14 00:00:00
abstract：OBJECTIVE:Autologous bone marrow stem cell (ABMSC) transplantation has been considered a promising option for hepatitis B virus-related cirrhosis (HBV-C). Although an analysis of the published literature has been performed, the exact effects and safety have yet to be systematically investigated. METHODS:We conducted a...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-01627-5
更新日期：2020-03-05 00:00:00
abstract：INTRODUCTION:Estrogen plays an important role in the osteogenic differentiation of mesenchymal stem cells, while stem cells from apical papilla (SCAP) can contribute to the formation of dentin/bone-like tissues. To date, the effects of estrogen on the differentiation of SCAP remain unclear. METHODS:SCAP was isolated a...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/scrt515
更新日期：2014-11-17 00:00:00
abstract：INTRODUCTION:Nervous system injuries comprise a diverse group of disorders that include traumatic brain injury (TBI). The potential of mesenchymal stem cells (MSCs) to differentiate into neural cell types has aroused hope for the possible development of autologous therapies for central nervous system injury. METHODS:I...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/scrt151
更新日期：2013-01-06 00:00:00
abstract：:Over the past decade a lot of research has been performed towards the therapeutic use of mesenchymal stem cells (MSCs) in neurodegenerative and neuroinflammatory diseases. MSCs have shown to be beneficial in different preclinical studies of central nervous system (CNS) disorders due to their immunomodulatory propertie...
journal_title：Stem cell research & therapy
pub_type： 杂志文章,评审
doi：10.1186/scrt312
更新日期：2013-08-22 00:00:00
abstract：INTRODUCTION:Fallopian tube, which is normally discarded in surgical procedures, has proven to be a source of mesenchymal stem cells (MSCs) with increasing evidence. However, fallopian tube mucosa, which can be acquired via non-invasive procedures, is a previously unknown source of MSCs. In the present study, we explor...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-015-0094-1
更新日期：2015-05-21 00:00:00
abstract：BACKGROUND:Acute myocardial infarction (MI) leads to an irreversible loss of proper cardiac function. Application of stem cell therapy is an attractive option for MI treatment. Adipose tissue has proven to serve as a rich source of stem cells (ADSCs). Taking into account the different morphogenesis, anatomy, and physio...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-016-0343-y
更新日期：2016-06-13 00:00:00
abstract：:The role of p53 as "a guardian of the genome" has been well established in somatic cells. However, its role in pluripotent stem cells remains much more elusive. Here, we discuss research progress in understanding the role of p53 in pluripotent stem cells and in pluripotent stem cell-like cancer stem cells. The p53 pro...
journal_title：Stem cell research & therapy
pub_type： 杂志文章,评审
doi：10.1186/s13287-017-0498-1
更新日期：2017-02-28 00:00:00
abstract：BACKGROUND:Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder that affects female fertility. However, with the lack of a corresponding research model, the pathology mechanism of PCOS is poorly understood. Induced pluripotent stem cell (iPSC) technology has been recognized as means to generate...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-018-0950-x
更新日期：2018-08-09 00:00:00
abstract：BACKGROUND:Within the last years, the interest in physical exercise as non-invasive stimulus influencing circulating hematopoietic stem and progenitor cell (CPC) concentrations has constantly grown. Cell estimates are often derived by determining the subgroup of CPC as percent lymphocytes (LYM) or mononuclear cells (MN...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-019-1403-x
更新日期：2019-10-17 00:00:00
abstract：INTRODUCTION:Chronic wounds are a major health-care issue, but research is limited by the complexity and heterogeneity in terms of wound etiology as well as patient-related factors. A suitable animal model that replicates the situation in humans is not available. Therefore, the aim of the present work is to present a s...
journal_title：Stem cell research & therapy
pub_type： 临床试验,杂志文章
doi：10.1186/scrt407
更新日期：2014-01-29 00:00:00
