Abstract：
BACKGROUND:Bone marrow mesenchymal stem cells (BMSCs) exhibit the capacity to self-renew and differentiate into multi-lineage cell types, including osteoblasts, which are crucial regulators of fracture healing. Thus, this study aims to investigate the effect of microRNA (miR)-22-3p from BMSC-derived EVs on osteogenic differentiation and its underlying mechanism. METHODS:Extracellular vesicles (EVs) were isolated from BMSCs and taken up with BMSCs. Dual-luciferase reporter gene assay was used to verify the binding relationship between miR-22-3p and FTO. Loss- and gain-of-function experiments were performed to determine the roles of EV-delivered miR-22-3p and FTO in osteogenic differentiation as well as their regulatory role in the MYC/PI3K/AKT axis. To determine the osteogenic differentiation, ALP and ARS stainings were conducted, and the levels of RUNX2, OCN, and OPN level were determined. In vivo experiment was conducted to determine the function of EV-delivered miR-22-3p and FTO in osteogenic differentiation, followed by ALP and ARS staining. RESULTS:miR-22-3p expression was repressed, while FTO expression was elevated in the ovariectomized mouse model. Overexpression of miR-22-3p, EV-delivered miR-22-3p, increased ALP activity and matrix mineralization of BMSCs and promoted RUNX2, OCN, and OPN expressions in BMSCs. miR-22-3p negatively targeted FTO expression. FTO silencing rescued the suppressed osteogenic differentiation by EV-delivered miR-22-3p inhibitor. FTO repression inactivated the MYC/PI3K/AKT pathway, thereby enhancing osteogenic differentiation both in vivo and in vitro. CONCLUSION:In summary, miR-22-3p delivered by BMSC-derived EVs could result in the inhibition of the MYC/PI3K/AKT pathway, thereby promoting osteogenic differentiation via FTO repression.
journal_name
Stem Cell Res Therjournal_title
Stem cell research & therapyauthors
Zhang X,Wang Y,Zhao H,Han X,Zhao T,Qu P,Li G,Wang Wdoi
10.1186/s13287-020-01707-6subject
Has Abstractpub_date
2020-06-10 00:00:00pages
227issue
1issn
1757-6512pii
10.1186/s13287-020-01707-6journal_volume
11pub_type
杂志文章abstract：BACKGROUND:The dog represents an excellent large animal model for translational cell-based studies. Importantly, the properties of canine multipotent stromal cells (cMSCs) and the ideal tissue source for specific translational studies have yet to be established. The aim of this study was to characterize cMSCs derived f...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-017-0639-6
更新日期：2017-10-03 00:00:00
abstract：BACKGROUND:Adult full-thickness cutaneous wound repair suffers from an imbalanced immune response, leading to nonfunctional reconstructed tissue and fibrosis. Although various treatments have been reported, the immune-mediated tissue regeneration driven by biomaterial offers an attractive regenerative strategy for dama...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-018-0768-6
更新日期：2018-01-31 00:00:00
abstract：INTRODUCTION:Fallopian tube, which is normally discarded in surgical procedures, has proven to be a source of mesenchymal stem cells (MSCs) with increasing evidence. However, fallopian tube mucosa, which can be acquired via non-invasive procedures, is a previously unknown source of MSCs. In the present study, we explor...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-015-0094-1
更新日期：2015-05-21 00:00:00
abstract：BACKGROUND:Sepsis is a global inflammatory disease that causes death. It has been reported that mesenchymal stem cell (MSC) treatment can attenuate inflammatory and septic symptoms. In this study, we investigated how interactions between neutrophils and human umbilical cord blood (hUCB)-MSCs in the liver of septic mice...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-1559-4
更新日期：2020-02-03 00:00:00
abstract：BACKGROUND:Alveolar bone loss is a frequent occurrence. Dental pulp stem cells (DPSCs) which have invasive accessibility and high osteogenic potential is a promising source for cell-based bone regeneration. EphrinB2 is involved in bone homeostasis and osteogenesis. The aim of this study was to investigate the effect an...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-019-1540-2
更新日期：2020-01-29 00:00:00
abstract：BACKGROUND:We aimed to generate a bioengineered multi-lamellar human corneal stroma tissue in vitro by differentiating periodontal ligament stem cells (PDLSCs) towards keratocytes on an aligned silk membrane. METHODS:Human PDLSCs were isolated and identified. The neuropeptide substance P (SP) was added in keratocyte d...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-017-0715-y
更新日期：2017-11-13 00:00:00
abstract：BACKGROUND:Graft-versus-host disease (GvHD) is the main life-threatening complication of allogeneic hematopoietic stem cell transplantation (HSCT). Thirty to 80% of GvHD patients do not respond to first-line treatment and a second-line treatment is not universally established. Based on their immunomodulatory properties...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-01592-z
更新日期：2020-02-18 00:00:00
abstract：:The original article [1] contains an error in Fig. 5 whereby sub-Fig. 5c, d & 5e are mistakenly mixed-up. ...
journal_title：Stem cell research & therapy
pub_type： 已发布勘误
doi：10.1186/s13287-020-1553-x
更新日期：2020-01-23 00:00:00
abstract：BACKGROUND:Bone marrow mesenchymal stem cells (BMSC) transfer has been attempted as a therapeutic strategy in experimental lung injury and fibrosis. Reduction of neutrophilic infiltration is one of the mechanisms involved in this effect. However, the mechanisms by which BMSC modulate neutrophil remains unknown. METHOD...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-018-0983-1
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abstract：OBJECTIVES:Mesenchymal stem cells (MSCs) have been intensively investigated as to their therapeutic potentials. However, the full chemical-defined medium supporting the isolation and expansion of human MSCs has not been developed yet. MATERIALS AND METHODS:Here, we developed the full chemical-defined medium, NBVbe med...
journal_title：Stem cell research & therapy
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journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-1550-0
更新日期：2020-01-23 00:00:00
abstract：BACKGROUND:Considerable progress has been made in converting human pluripotent stem cells (hPSCs) into cortical neurons for disease modeling and regenerative medicine. However, these procedures are hard to provide sufficient cells for their applications. Using a combination of small-molecules and growth factors, we pre...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-01939-6
更新日期：2020-10-02 00:00:00
abstract：:Natural killer (NK) cells play a crucial role in host immunity by detecting cells that downregulate MHC class I presentation and upregulate stress ligands, as commonly seen in cancers. Current NK therapies using primary NK cells are prone to manufacturing issues related to expansion and storage. Alternative cell sourc...
journal_title：Stem cell research & therapy
pub_type： 杂志文章,评审
doi：10.1186/s13287-020-01741-4
更新日期：2020-06-16 00:00:00
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journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-019-1519-z
更新日期：2020-01-03 00:00:00
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journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-018-1054-3
更新日期：2018-11-21 00:00:00
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journal_title：Stem cell research & therapy
pub_type： 评论,杂志文章
doi：10.1186/s13287-015-0215-x
更新日期：2015-11-04 00:00:00
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journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-016-0432-y
更新日期：2016-12-01 00:00:00
abstract：:Somatic cell nuclear transfer (SCNT) has shown a wide application in the generation of transgenic animals, protection of endangered animals, and therapeutic cloning. However, the efficiency of SCNT remains very low due to some poorly characterized key factors. Compared with fertilized embryos, somatic donor cells lack...
journal_title：Stem cell research & therapy
pub_type： 杂志文章,评审
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更新日期：2020-02-18 00:00:00
abstract：:The original article [1] contained errors in the presentation of Figure 7. ...
journal_title：Stem cell research & therapy
pub_type： 已发布勘误
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journal_title：Stem cell research & therapy
pub_type： 杂志文章,评审
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journal_title：Stem cell research & therapy
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更新日期：2015-08-22 00:00:00
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journal_title：Stem cell research & therapy
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更新日期：2016-05-23 00:00:00
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journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-017-0521-6
更新日期：2017-03-11 00:00:00
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journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-016-0410-4
更新日期：2016-09-29 00:00:00
abstract：BACKGROUND:Due to their intrinsic properties, stem cells are promising tools for new developments in tissue engineering and particularly for cartilage tissue regeneration. Although mesenchymal stromal/stem cells from bone marrow (BM-MSC) have long been the most used stem cell source in cartilage tissue engineering, the...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-015-0263-2
更新日期：2015-12-30 00:00:00
abstract：BACKGROUND:Type 1 diabetes (T1D) is an autoimmune disease resulting from the destruction of insulin-secreting islet β cells by autoreactive T cells. Non-obese diabetic (NOD) mice are the widely used animal model for human T1D. Autoimmunity in NOD mice is associated with particular major histocompatibility complex (MHC)...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-019-1347-1
更新日期：2019-08-06 00:00:00
abstract：BACKGROUND:Epidermal grafting using cells derived from pluripotent stem cells will change the face of this side of regenerative cutaneous medicine. To date, the safety of the graft would be the major unmet deal in order to implement long-term skin grafting. In this context, experiments on large animals appear unavoidab...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-017-0741-9
更新日期：2017-12-19 00:00:00
abstract：BACKGROUND:Patients suffering from osteoporosis show an increased number of adipocytes in their bone marrow, concomitant with a reduction in the pool of human mesenchymal stem cells (hMSCs) that are able to differentiate into osteoblasts, thus leading to suppressed osteogenesis. METHODS:In order to be able to interfer...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-016-0375-3
更新日期：2016-08-26 00:00:00
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journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-018-1090-z
更新日期：2018-12-29 00:00:00
abstract：INTRODUCTION:Stem cell therapy has emerged as potential therapeutic strategy for damaged heart muscles. Umbilical cord blood (UCB) cells are the most prevalent stem cell source available, yet have not been fully tested in cardiac regeneration. Herein, studies were performed to evaluate the cardiovascular safety and ben...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-015-0044-y
更新日期：2015-03-26 00:00:00
