Abstract:
:Somatic cell nuclear transfer (SCNT) has shown a wide application in the generation of transgenic animals, protection of endangered animals, and therapeutic cloning. However, the efficiency of SCNT remains very low due to some poorly characterized key factors. Compared with fertilized embryos, somatic donor cells lack some important components of sperm, such as sperm small noncoding RNA (sncRNA) and proteins. Loss of these factors is considered an important reason for the abnormal development of SCNT embryo. This study focused on recent advances of SCNT and the roles of sperm in development. Sperm-derived factors play an important role in nucleus reprogramming and cytoskeleton remodeling during SCNT embryo development. Hence, considering the role of sperm may provide a new strategy for improving cloning efficiency.
journal_name
Stem Cell Res Therjournal_title
Stem cell research & therapyauthors
Qu P,Wang Y,Zhang C,Liu Edoi
10.1186/s13287-020-01599-6subject
Has Abstractpub_date
2020-02-18 00:00:00pages
65issue
1issn
1757-6512pii
10.1186/s13287-020-01599-6journal_volume
11pub_type
杂志文章,评审abstract:BACKGROUND:In regenerative medicine the maintenance of stem cell properties is of crucial importance. Ageing is considered a cause of reduced stemness capability. The limbus is a stem niche of easy access and harbors two stem cell populations: epithelial stem cells and fibroblast-like stem cells. Our aim was to investi...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-016-0342-z
更新日期:2016-06-13 00:00:00
abstract:BACKGROUND:Mesenchymal stromal/stem cells (MSCs) have demonstrated pro-healing properties due to their anti-inflammatory, angiogenic, and even antibacterial properties. We have shown previously that minocycline enhances the wound healing phenotype of MSCs, and MSCs encapsulated in poly(ethylene glycol) and gelatin-base...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-017-0623-1
更新日期:2017-07-21 00:00:00
abstract:INTRODUCTION:Despite attempts to prevent brain injury during the hyperacute phase of stroke, most sufferers end up with significant neuronal loss and functional deficits. The use of cell-based therapies to recover the injured brain offers new hope. In the current study, we employed human neural stem cells (hNSCs) isola...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-015-0175-1
更新日期:2015-09-29 00:00:00
abstract:BACKGROUND:Mesenchymal stromal cells (MSCs) are considered potential candidates that hold great promise in the treatment of immune-related diseases. For therapeutic applications, it is necessary to isolate and expand MSCs with procedures complying with good manufacturing practice (GMP). Recent studies reported the use ...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-019-1175-3
更新日期:2019-03-07 00:00:00
abstract:BACKGROUND:Degeneration of smooth muscles in sphincters can cause debilitating diseases such as fecal incontinence. Skeletal muscle-derived cells have been effectively used in clinics for the regeneration of the skeletal muscle sphincters, such as the external anal or urinary sphincter. However, little is known about t...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-020-01749-w
更新日期:2020-06-12 00:00:00
abstract:INTRODUCTION:Short-term neural stem cell (NSC) transplantation improves cognition in Alzheimer's disease (AD) transgenic mice by enhancing endogenous synaptic connectivity. However, this approach has no effect on the underlying beta-amyloid (Aβ) and neurofibrillary tangle pathology. Long term efficacy of cell based app...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/scrt440
更新日期:2014-04-16 00:00:00
abstract:BACKGROUND:Severe acute pancreatitis (SAP) is an acute abdominal disease characterized by pancreatic necrosis and systemic disease. In a previous study, we showed that bone marrow-derived mesenchymal stem cells (BMSCs) can reduce SAP by secreting microRNA (miR)-9; however, the underlying mechanism remains unclear. The ...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-018-1022-y
更新日期:2018-10-25 00:00:00
abstract:BACKGROUND:The intervertebral disc (IVD) has limited self-healing potential and disc repair strategies require an appropriate cell source such as progenitor cells that could regenerate the damaged cells and tissues. The objective of this study was to identify nucleus pulposus-derived progenitor cells (NPPC) and examine...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-016-0337-9
更新日期:2016-05-23 00:00:00
abstract:BACKGROUND:A keloid is a fibroproliferative disorder occurring in wounds characterized by an exaggerated response to injury. To date, no effective cure has been identified. As multipotent stem cells, human adipose-derived stem cells (ADSCs) may show the possibility for curing diseases such as fibrosis. This study sough...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-018-0786-4
更新日期:2018-02-21 00:00:00
abstract:INTRODUCTION:The bursa subacromialis (BS) provides the gliding mechanism of the shoulder and regenerates itself after surgical removal. Therefore, we explored the presence of mesenchymal stem cells (MSCs) within the human adult BS tissue and characterized the BS cells compared to MSCs from bone marrow (BMSCs) on a mole...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-015-0104-3
更新日期:2015-06-03 00:00:00
abstract:BACKGROUND:Critical limb ischemia (CLI) constitutes the most aggressive form of peripheral arterial occlusive disease, characterized by the blockade of arteries supplying blood to the lower extremities, significantly diminishing oxygen and nutrient supply. CLI patients usually undergo amputation of fingers, feet, or ex...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-020-01591-0
更新日期:2020-03-06 00:00:00
abstract::Parkinson's disease (PD) is a neurodegenerative disorder characterized by the progressive accumulation of Lewy body inclusions along with selective destruction of dopaminergic (DA) neurons in the nigrostriatal tract of the brain. Genetic studies have revealed much about the pathophysiology of PD, enabling the identifi...
journal_title:Stem cell research & therapy
pub_type: 杂志文章,评审
doi:10.1186/scrt102
更新日期:2012-04-03 00:00:00
abstract:BACKGROUND:Stress urinary incontinence (SUI) is a common and bothersome condition. Invasive surgery will always be considered after conservative treatment fails, but the rates of postoperative complications and long-term recurrence are high. Thus, a new treatment strategy is still needed. In recent years, bone marrow m...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-020-01808-2
更新日期:2020-07-16 00:00:00
abstract:BACKGROUND:Considerable progress has been made in converting human pluripotent stem cells (hPSCs) into cortical neurons for disease modeling and regenerative medicine. However, these procedures are hard to provide sufficient cells for their applications. Using a combination of small-molecules and growth factors, we pre...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-020-01939-6
更新日期:2020-10-02 00:00:00
abstract::The original article [1] contained errors in the presentation of Figure 7. ...
journal_title:Stem cell research & therapy
pub_type: 已发布勘误
doi:10.1186/s13287-018-1116-6
更新日期:2019-01-08 00:00:00
abstract::Tissue engineering has developed many paradigms and techniques on how to best integrate cells and extracellular matrix to create in vitro structures that replicate native tissue. The strategy best suited for building these constructs depends mainly on the target cells, tissues, and organ of interest, and how readily t...
journal_title:Stem cell research & therapy
pub_type: 杂志文章,评审
doi:10.1186/scrt162
更新日期:2013-02-14 00:00:00
abstract:BACKGROUND:Optimal cell type as cell-based therapies for heart failure (HF) remains unclear. We sought to compare the safety and efficacy of direct intramyocardial transplantation of human embryonic stem cell-derived cardiomyocytes (hESC-CMs) and human induced pluripotent stem cell-derived mesenchymal stem cells (hiPSC...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-019-1183-3
更新日期:2019-03-07 00:00:00
abstract:BACKGROUND:Graft-versus-host disease (GvHD) is the main life-threatening complication of allogeneic hematopoietic stem cell transplantation (HSCT). Thirty to 80% of GvHD patients do not respond to first-line treatment and a second-line treatment is not universally established. Based on their immunomodulatory properties...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-020-01592-z
更新日期:2020-02-18 00:00:00
abstract:BACKGROUND:Precision or individualized medicine is increasingly pervasive across medical and surgical disciplines. However, the concept has not been introduced in regenerative medicine. MAIN BODY:Targeted and engineered cellular and acellular therapy, specific to individuals or disease states, could significantly impr...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-020-02092-w
更新日期:2021-01-07 00:00:00
abstract:BACKGROUND:WNT and TGFβ signaling pathways play critical regulatory roles in cardiomyocyte fate determination and differentiation. MiRNAs are also known to regulate different biological processes and signaling pathways. Here, we intended to find candidate miRNAs that are involved in cardiac differentiation through regu...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-019-1249-2
更新日期:2019-06-27 00:00:00
abstract:BACKGROUND:Lung cancer stem cells have the ability to self-renew and are resistant to conventional chemotherapy. MicroRNAs (miRNAs) regulate and control the expression and function of many target genes; therefore, miRNA disorders are involved in the pathogenesis of human diseases, such as cancer. However, the effects o...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-018-1072-1
更新日期:2018-11-23 00:00:00
abstract:BACKGROUND:Mesenchymal stem cells (MSCs) are a heterogeneous subset of stromal cells currently tested for multiple therapeutic purposes. Their potential to home into tumors, to secrete trophic/vasculogenic factors, and to suppress immune response raises questions regarding their biosafety. Our aim was to evaluate wheth...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-018-0878-1
更新日期:2018-05-11 00:00:00
abstract:BACKGROUND:Spermatogonial stem cell transplantation (SSCT) could become a fertility restoration tool for childhood cancer survivors. However, since in mice, the colonization efficiency of transplanted spermatogonial stem cells (SSCs) is only 12%, the efficiency of the procedure needs to be improved before clinical impl...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-018-1065-0
更新日期:2018-11-21 00:00:00
abstract::Tissues characterized by constant turnover contain post-mitotic, terminally differentiated cells originating from highly proliferative progenitors, which in turn derive from a relatively small population of stem cells. At the population level, self-renewal and differentiation are the possible outcomes of stem cell pro...
journal_title:Stem cell research & therapy
pub_type: 杂志文章,评审
doi:10.1186/scrt101
更新日期:2012-03-19 00:00:00
abstract:BACKGROUND:The therapeutic effect of mesenchymal stem cells (MSCs) from human adipose tissue on renal interstitial fibrosis has been demonstrated by several groups. However, the way to enhance the renoprotective effect of adipose-derived mesenchymal stem cells (AMSCs) and the possible mechanisms are still unclear. The ...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-020-02049-z
更新日期:2021-01-07 00:00:00
abstract::Paneth cells (PCs) are located at the bottom of small intestinal crypts and play an important role in maintaining the stability of the intestinal tract. Previous studies reported on how PCs shape the intestinal microbiota or the response to the immune system. Recent studies have determined that PCs play an important r...
journal_title:Stem cell research & therapy
pub_type: 杂志文章,评审
doi:10.1186/s13287-020-01857-7
更新日期:2020-08-12 00:00:00
abstract::The efficacy of tendon-derived stem cells (TDSCs) for the promotion of tendon and tendon-bone junction repair has been reported in animal studies. Modulation of the tendon stem cell niche in vivo has also been reported to influence tendon structure. There is a need to have specific and reliable markers that can define...
journal_title:Stem cell research & therapy
pub_type: 杂志文章,评审
doi:10.1186/s13287-015-0097-y
更新日期:2015-06-02 00:00:00
abstract:BACKGROUND:Exploring the effects of lncRNA SNHG1 in the process of osteogenic differentiation of periodontal ligament stem cells (PDLSCs) would provide novel therapeutic strategies for tissue regeneration. METHODS:Loss-of-function and gain-of-function assays were induced by lentivirus. The osteogenic differentiation o...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-020-01953-8
更新日期:2020-10-07 00:00:00
abstract::The original article [1] contains an error in Fig. 5 whereby sub-Fig. 5c, d & 5e are mistakenly mixed-up. ...
journal_title:Stem cell research & therapy
pub_type: 已发布勘误
doi:10.1186/s13287-020-1553-x
更新日期:2020-01-23 00:00:00
abstract:BACKGROUND:Due to their intrinsic properties, stem cells are promising tools for new developments in tissue engineering and particularly for cartilage tissue regeneration. Although mesenchymal stromal/stem cells from bone marrow (BM-MSC) have long been the most used stem cell source in cartilage tissue engineering, the...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/s13287-015-0263-2
更新日期:2015-12-30 00:00:00