Abstract：
BACKGROUND:Using a toxin-induced nonhuman primate model of acute liver failure (ALF), we previously reported that peripheral infusion of human umbilical cord mesenchymal stem cells (hUC-MSCs) strongly suppresses the activation of circulating monocytes and interleukin-6 (IL-6) production, thereby disrupting the development of a cytokine storm and improving the prognosis of monkeys. MSCs are considered to play a therapeutic role under different stresses by adaptively producing specific factors, prompting us to investigate the factors that hUC-MSCs produce in response to high serum levels of IL-6, which plays a critical role in initiating and accelerating ALF. METHODS:We stimulated hUC-MSCs with IL-6, and the hUC-MSC-derived exosomes were deeply sequenced. The miRNAs in the exosomes that have potential to suppress IL-6-associated signaling pathway were screened, and the role of one of the most possible miRNAs was tested in the mouse model of inflammatory liver injury. RESULT:We determined that miR-455-3p, which is secreted through exosomes and potentially targets PI3K signaling, was highly produced by hUC-MSCs with IL-6 stimulation. The miR-455-3p-enriched exosomes could inhibit the activation and cytokine production of macrophages challenged with lipopolysaccharide (LPS) both in vivo and in vitro. In a chemical liver injury mouse model, enforced expression of miR-455-3p could attenuate macrophage infiltration and local liver damage and reduce the serum levels of inflammatory factors, thereby improving liver histology and systemic disorder. CONCLUSIONS:miR-455-3p-enriched exosomes derived from hUC-MSCs are a promising therapy for acute inflammatory liver injury.
journal_name
Stem Cell Res Therjournal_title
Stem cell research & therapyauthors
Shao M,Xu Q,Wu Z,Chen Y,Shu Y,Cao X,Chen M,Zhang B,Zhou Y,Yao R,Shi Y,Bu Hdoi
10.1186/s13287-020-1550-0subject
Has Abstractpub_date
2020-01-23 00:00:00pages
37issue
1issn
1757-6512pii
10.1186/s13287-020-1550-0journal_volume
11pub_type
杂志文章abstract：BACKGROUND:As a type of high-frequency electrotherapy, a short-wave can promote the fracture healing process; yet, its underlying therapeutic mechanisms remain unclear. PURPOSE:To observe the effect of Short-Wave therapy on mesenchymal stem cell (MSC) homing and relative mechanisms associated with fracture healing. M...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-01888-0
更新日期：2020-09-07 00:00:00
abstract：BACKGROUND:We aimed to generate a bioengineered multi-lamellar human corneal stroma tissue in vitro by differentiating periodontal ligament stem cells (PDLSCs) towards keratocytes on an aligned silk membrane. METHODS:Human PDLSCs were isolated and identified. The neuropeptide substance P (SP) was added in keratocyte d...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-017-0715-y
更新日期：2017-11-13 00:00:00
abstract：BACKGROUND:miRNA expression profiles in ectopic endometrium (EC) serving as pathophysiologic genetic fingerprints contribute to determining endometriosis progression; however, the underlying molecular mechanisms remain unknown. METHODS:miRNA microarray analysis was used to determine the expression profiling of EC fres...
journal_title：Stem cell research & therapy
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abstract：INTRODUCTION:Topical administration of eye drops is the major route for drug delivery to the cornea. Orbital fat-derived stem cells (OFSCs) possess an in vitro corneal epithelial differentiation capacity. Both the safety and immunomodulatory ability of systemic OFSC transplantation were demonstrated in our previous wor...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/scrt223
更新日期：2013-06-14 00:00:00
abstract：BACKGROUND:The skeletal muscle regeneration relays on the satellite cells which are stem cells located between basal lamina and plasmalemma of muscle fiber. In the injured muscles, the satellite cells become activated, start to proliferate, and then differentiate into myoblasts, which fuse to form myotubes and finally ...
journal_title：Stem cell research & therapy
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doi：10.1186/s13287-020-01827-z
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journal_title：Stem cell research & therapy
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doi：10.1186/s13287-015-0104-3
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abstract：BACKGROUND:Due to limitations of current angiogenesis assays, we aimed to develop a novel application of the rat aortic ring assay to assess the angiogenic potential of mesenchymal stromal cells (MSCs). First-trimester human umbilical cord-derived perivascular cells (FTM HUCPVCs) have multipotent characteristics and pr...
journal_title：Stem cell research & therapy
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doi：10.1186/s13287-017-0631-1
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abstract：BACKGROUND:Sepsis is a global inflammatory disease that causes death. It has been reported that mesenchymal stem cell (MSC) treatment can attenuate inflammatory and septic symptoms. In this study, we investigated how interactions between neutrophils and human umbilical cord blood (hUCB)-MSCs in the liver of septic mice...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-1559-4
更新日期：2020-02-03 00:00:00
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journal_title：Stem cell research & therapy
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journal_title：Stem cell research & therapy
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journal_title：Stem cell research & therapy
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journal_title：Stem cell research & therapy
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abstract：BACKGROUND:Osteoarthritis (OA) is a major cause of limb dysfunction, and distraction arthroplasty which generates intermittent hydrostatic pressure (IHP) is an effective approach for OA treatment. However, the result was not always satisfactory and the reasons remained unresolved. Because aging is recognized as an impo...
journal_title：Stem cell research & therapy
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doi：10.1186/s13287-020-01708-5
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journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/scrt436
更新日期：2014-04-10 00:00:00
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journal_title：Stem cell research & therapy
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doi：10.1186/scrt35
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journal_title：Stem cell research & therapy
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更新日期：2018-01-10 00:00:00
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journal_title：Stem cell research & therapy
pub_type： 杂志文章,评审
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更新日期：2021-01-19 00:00:00
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journal_title：Stem cell research & therapy
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doi：10.1186/s13287-017-0577-3
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journal_title：Stem cell research & therapy
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journal_title：Stem cell research & therapy
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journal_title：Stem cell research & therapy
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journal_title：Stem cell research & therapy
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更新日期：2013-10-11 00:00:00
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journal_title：Stem cell research & therapy
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journal_title：Stem cell research & therapy
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doi：10.1186/s13287-018-0984-0
更新日期：2018-09-21 00:00:00
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journal_title：Stem cell research & therapy
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doi：10.1186/s13287-019-1347-1
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journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/scrt418
更新日期：2014-02-26 00:00:00
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journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-015-0016-2
更新日期：2015-03-13 00:00:00
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journal_title：Stem cell research & therapy
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doi：10.1186/s13287-018-1106-8
更新日期：2019-01-03 00:00:00
abstract：BACKGROUND:Nitric oxide (NO) plays a role in a number of physiological processes including stem cell differentiation and osteogenesis. Endothelial nitric oxide synthase (eNOS), one of three NO-producing enzymes, is located in a close conformation with the caveolin-1 (CAV-1WT) membrane protein which is inhibitory to NO ...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-016-0442-9
更新日期：2016-12-07 00:00:00
