Abstract：
BACKGROUND:miRNA expression profiles in ectopic endometrium (EC) serving as pathophysiologic genetic fingerprints contribute to determining endometriosis progression; however, the underlying molecular mechanisms remain unknown. METHODS:miRNA microarray analysis was used to determine the expression profiling of EC fresh tissues. qRT-PCR was performed to screen miR-205-5p expression in EC tissues. The roles of miR-205-5p and its candidate target gene, angiopoietin-2 (ANGPT2), in endometriosis progression were confirmed on the basis of both in vitro and in vivo systems. miR-205-5p and ANGPT2 expression were measured by in situ hybridization and immunochemistry, and their clinical significance was statistically analysed. RESULTS:miR-205-5p was screened as a novel suppressor of endometriosis through primary ectopic endometrial stromal cell migration, invasion, and apoptosis assay in vitro, along with endometrial-like xenograft growth and apoptosis in vivo. In addition, ANGPT2 was identified as a direct target of miR-205-5p through bioinformatic target prediction and luciferase reporter assay. Re-expression and knockdown of ANGPT2 could respectively rescue and simulate the effects induced by miR-205-5p. Importantly, the miR-205-5p-ANGPT2 axis was found to activate the ERK/AKT pathway in endometriosis. Finally, miR-205-5p and ANGPT2 expression were closely correlated with the endometriosis severity. CONCLUSION:The newly identified miR-205-5p-ANGPT2-AKT/ERK axis illustrates the molecular mechanism of endometriosis progression and may represent a novel diagnostic biomarker and therapeutic target for disease treatment.
journal_name
Stem Cell Res Therjournal_title
Stem cell research & therapyauthors
Zhou CF,Liu MJ,Wang W,Wu S,Huang YX,Chen GB,Liu LM,Peng DX,Wang XF,Cai XZ,Li XX,Feng WQ,Ma Ydoi
10.1186/s13287-019-1388-5subject
Has Abstractpub_date
2019-09-23 00:00:00pages
287issue
1issn
1757-6512pii
10.1186/s13287-019-1388-5journal_volume
10pub_type
杂志文章abstract：BACKGROUND:For patients with thromboangiitis obliterans (TAO), revascularization with bypass or angioplasty is frequently not feasible due to the poor outflow of the distal small vessels. We evaluated the long-term results of our experience treating patients with TAO with autologous bone marrow-derived mononuclear cell...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-018-0784-6
更新日期：2018-02-22 00:00:00
abstract：BACKGROUND:Human adipose-derived stem cells (hASCs) are a subset of mesenchymal stem cells (MSCs); it has been regarded as one of the most promising stem cells. We previously found that fibroblast growth factor-2 (FGF-2) enhanced the proliferation and differentiation of hASC. However, the mechanisms involved in the gro...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-019-1462-z
更新日期：2019-11-27 00:00:00
abstract：:Stem cell therapy has been applied in many fields. Basic and clinical studies on stem cell therapy for acute kidney injury (AKI) have been conducted. Stem cells have been found to exert renal protection through a variety of mechanisms, such as regulating the immune system and secreting growth factors, cytokines, and e...
journal_title：Stem cell research & therapy
pub_type： 杂志文章,评审
doi：10.1186/s13287-019-1371-1
更新日期：2019-09-03 00:00:00
abstract：BACKGROUND:Although adult human tissue-derived epidermal stem cells are capable of differentiating into enamel-secreting ameloblasts and forming teeth with regenerated enamel when recombined with mouse dental mesenchyme that possesses odontogenic potential, the induction rate is relatively low. In addition, whether the...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-018-0822-4
更新日期：2018-05-02 00:00:00
abstract：BACKGROUND:Urine-derived stem cells (USCs) are a valuable stem cell source for tissue engineering because they can be harvested non-invasively. Small intestine submucosa (SIS) has been used as scaffolds for soft tissue repair in the clinic. However, the feasibility and efficacy of a combination of USCs and SIS for skin...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-01662-2
更新日期：2020-04-06 00:00:00
abstract：:On 23 May 2013, the Italian health minister decided to endorse a law regarding controversial stem cell-based therapies in 32 young terminally ill patients, shocking many scientists. According to the international scientific community, these therapies could be dangerous because they were not rigorously tested in humans...
journal_title：Stem cell research & therapy
pub_type： 杂志文章,评审
doi：10.1186/scrt333
更新日期：2013-10-11 00:00:00
abstract：BACKGROUND:Dystrophic epidermolysis bullosa (DEB), a rare genodermatosis, is characterized by the formation of intra-epidermal blistering and the development of chronic nonhealing skin wounds. Recently, attempts have been made to develop cell-based therapies for this currently intractable disorder. The molecular mechan...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-016-0388-y
更新日期：2016-08-27 00:00:00
abstract：INTRODUCTION:Endothelial dysfunction is found in different pathologies such as diabetes and renal and heart diseases, representing one of the major health problems. The reduced vasodilation of impaired endothelium starts a prothrombotic state associated with irregular blood flow. We aimed to explore the potential of am...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-015-0204-0
更新日期：2015-10-31 00:00:00
abstract：INTRODUCTION:Studies have demonstrated that mesenchymal stromal cells (MSCs) could reverse acute and chronic kidney injury by a paracrine or endocrine mechanism, and microvesicles (MVs) have been regarded as a crucial means of intercellular communication. In the current study, we focused on the therapeutic effects of h...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/scrt428
更新日期：2014-03-19 00:00:00
abstract：BACKGROUND:Liver fibrosis resulting from chronic liver injury is one of the major causes of mortality worldwide. Stem cell-secreted secretome has been evaluated for overcoming the limitations of cell-based therapy in hepatic disease, while maintaining its advantages. METHODS:In this study, we investigated the effect o...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-01891-5
更新日期：2020-09-03 00:00:00
abstract：:Adipogenesis is essential in in vitro experimentation to assess differentiation capability of stem cells, and therefore, its accurate measurement is important. Quantitative analysis of adipogenic levels, however, is challenging and often susceptible to errors due to non-specific reading or manual estimation by observe...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-019-1141-0
更新日期：2019-01-22 00:00:00
abstract：INTRODUCTION:Human hematopoietic stem cells (HSCs) have been clinically used for transplantation and gene and cellular therapy for more than 4 decades. However, this use is limited because of the challenges in the ex vivo culturing of HSCs. The major hurdle is to amplify these cells without losing their self-renewing p...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/scrt208
更新日期：2013-05-24 00:00:00
abstract：INTRODUCTION:Neural stem cells (NSCs) are among the most promising candidates for cell replacement therapy in neuronal injury and neurodegenerative diseases. One of the remaining obstacles for NSC therapy is to overcome the alloimmune response on NSCs by the host. METHODS:To investigate the mechanisms of immune modula...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/scrt206
更新日期：2013-05-21 00:00:00
abstract：BACKGROUND:Severe acute pancreatitis (SAP) is an acute abdominal disease characterized by pancreatic necrosis and systemic disease. In a previous study, we showed that bone marrow-derived mesenchymal stem cells (BMSCs) can reduce SAP by secreting microRNA (miR)-9; however, the underlying mechanism remains unclear. The ...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-018-1022-y
更新日期：2018-10-25 00:00:00
abstract：BACKGROUND:Patients suffering from osteoporosis show an increased number of adipocytes in their bone marrow, concomitant with a reduction in the pool of human mesenchymal stem cells (hMSCs) that are able to differentiate into osteoblasts, thus leading to suppressed osteogenesis. METHODS:In order to be able to interfer...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-016-0375-3
更新日期：2016-08-26 00:00:00
abstract：INTRODUCTION:Impaction allograft with cement is a common technique used in revision hip surgeries for the last 20 years. However, its clinical results are inconsistent. Recent studies have shown that mesenchymal stem cells (MSCs) seeded onto allograft can enhance bone formation. This in vitro study investigates whether...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/scrt350
更新日期：2013-01-01 00:00:00
abstract：INTRODUCTION:Stem cell therapy has emerged as potential therapeutic strategy for damaged heart muscles. Umbilical cord blood (UCB) cells are the most prevalent stem cell source available, yet have not been fully tested in cardiac regeneration. Herein, studies were performed to evaluate the cardiovascular safety and ben...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-015-0044-y
更新日期：2015-03-26 00:00:00
abstract：INTRODUCTION:Mesenchymal stem cells (MSCs) are adult, multipotent, stem cells with immunomodulatory properties. The mechanisms involved in the capacity of MSCs to inhibit the proliferation of proinflammatory T lymphocytes, which appear responsible for causing autoimmune disease, have yet to be fully elucidated. One of ...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/scrt216
更新日期：2013-06-04 00:00:00
abstract：INTRODUCTION:This is the first study evaluating the interactions of human adipose tissue derived stem cells (ADSCs) and human squamous cell carcinoma cells (SCCs), with regard to a prospective cell-based skin regenerative therapy and a thereby unintended co-localization of ADSCs and SCCs. METHODS:ADSCs were co-culture...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/scrt454
更新日期：2014-05-12 00:00:00
abstract：BACKGROUND:Apelin plays a key beneficial role in energy metabolism by increasing glucose uptake and insulin sensitivity; however, apelin has a short half-life because it is rapidly cleared from the circulation limiting its therapeutic benefit. The aim of this study is to create a new approach to treat type 2 diabetes b...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-018-1084-x
更新日期：2018-12-07 00:00:00
abstract：BACKGROUND:Acute liver failure (ALF) is a complicated condition that is characterized by global hepatocyte death and often requires immediate liver transplantation. However, this therapy is limited by shortage of donor organs. Mesenchymal stem cells (MSCs) and hepatocytes are two attractive sources of cell-based therap...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-01962-7
更新日期：2020-10-23 00:00:00
abstract：BACKGROUND:Alveolar bone loss is a frequent occurrence. Dental pulp stem cells (DPSCs) which have invasive accessibility and high osteogenic potential is a promising source for cell-based bone regeneration. EphrinB2 is involved in bone homeostasis and osteogenesis. The aim of this study was to investigate the effect an...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-019-1540-2
更新日期：2020-01-29 00:00:00
abstract：INTRODUCTION:The bursa subacromialis (BS) provides the gliding mechanism of the shoulder and regenerates itself after surgical removal. Therefore, we explored the presence of mesenchymal stem cells (MSCs) within the human adult BS tissue and characterized the BS cells compared to MSCs from bone marrow (BMSCs) on a mole...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-015-0104-3
更新日期：2015-06-03 00:00:00
abstract：:The role of p53 as "a guardian of the genome" has been well established in somatic cells. However, its role in pluripotent stem cells remains much more elusive. Here, we discuss research progress in understanding the role of p53 in pluripotent stem cells and in pluripotent stem cell-like cancer stem cells. The p53 pro...
journal_title：Stem cell research & therapy
pub_type： 杂志文章,评审
doi：10.1186/s13287-017-0498-1
更新日期：2017-02-28 00:00:00
abstract：BACKGROUND:Although endothelial progenitor cells (EPCs) contribute to wound repair by promoting neovascularization, the mechanism of EPC-mediated wound healing remains poorly understood due to the lack of pivotal molecular targets of dermal wound repair. METHODS AND RESULTS:We found that genetic targeting of the Lnk g...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-016-0403-3
更新日期：2016-10-28 00:00:00
abstract：BACKGROUND:Many studies have shown that long noncoding RNAs (lncRNAs) are closely related to the stimulation of osteogenic differentiation of adipose-derived stem cells (ADSCs) and the prevention of osteoporosis. Current research aimed to investigate the novel lncRNA and explored the function and molecular mechanism of...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-01754-z
更新日期：2020-06-17 00:00:00
abstract：BACKGROUND:Bone marrow-derived cell therapy has been used to treat acute myocardial infarction. However, the therapeutic efficacy of this approach remains controversial. Here, we performed a systematic review and meta-analysis to evaluate short-term and long-term effectiveness of bone marrow-derived therapy. METHODS:W...
journal_title：Stem cell research & therapy
pub_type： 杂志文章,meta分析
doi：10.1186/s13287-016-0415-z
更新日期：2016-10-20 00:00:00
abstract：INTRODUCTION:Octamer-binding transcription factor 4 (Oct4) is a master regulator of early mammalian development. Its expression begins from the oocyte stage, becomes restricted to the inner cell mass of the blastocyst and eventually remains only in primordial germ cells. Unearthing the interactions of Oct4 would provid...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/scrt67
更新日期：2011-05-13 00:00:00
abstract：BACKGROUND:Islets of Langerhans transplantation is a promising therapy for type 1 diabetes mellitus, but this technique is compromised by transplantation stresses including inflammation. In other tissues, co-transplantation with mesenchymal stem cells has been shown to reduce damage by improving anti-inflammatory and a...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-019-1190-4
更新日期：2019-03-12 00:00:00
abstract：INTRODUCTION:Estrogen plays an important role in the osteogenic differentiation of mesenchymal stem cells, while stem cells from apical papilla (SCAP) can contribute to the formation of dentin/bone-like tissues. To date, the effects of estrogen on the differentiation of SCAP remain unclear. METHODS:SCAP was isolated a...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/scrt515
更新日期：2014-11-17 00:00:00
