Microvesicles derived from human Wharton's Jelly mesenchymal stromal cells ameliorate renal ischemia-reperfusion injury in rats by suppressing CX3CL1.


INTRODUCTION:Studies have demonstrated that mesenchymal stromal cells (MSCs) could reverse acute and chronic kidney injury by a paracrine or endocrine mechanism, and microvesicles (MVs) have been regarded as a crucial means of intercellular communication. In the current study, we focused on the therapeutic effects of human Wharton-Jelly MSCs derived microvesicles (hWJMSC-MVs) in renal ischemia/reperfusion injury and its potential mechanisms. METHODS:MVs isolated from conditioned medium were injected intravenously in rats immediately after ischemia of the left kidney for 60 minutes. The animals were sacrificed at 24 hours, 48 hours and 2 weeks after reperfusion. The infiltration of inflammatory cells was identified by the immunostaining of CD68+ cells. ELISA was employed to determine the inflammatory factors in the kidney and serum von Willebrand Factor (VWF). Tubular cell proliferation and apoptosis were identified by immunostaining. Renal fibrosis was assessed by Masson's tri-chrome straining and alpha-smooth muscle actin (α-SMA) staining. The CX3CL1 expression in the kidney was measured by immunostaining and Western blot, respectively. In vitro, human umbilical vein endothelial cells were treated with or without MVs for 24 or 48 hours under hypoxia injury to test the CX3CL1 by immunostaining and Western blot. RESULTS:After administration of hWJMSC-MVs in acute kidney injury (AKI) rats, renal cell apoptosis was mitigated and proliferation was enhanced, inflammation was also alleviated in the first 48 hours. MVs also could suppress the expression of CX3CL1 and decrease the number of CD68+ macrophages in the kidney. In the late period, improvement of renal function and abrogation of renal fibrosis were observed. In vitro, MVs could down-regulate the expression of CX3CL1 in human umbilical vein endothelial cells under hypoxia injury at 24 or 48 hours. CONCLUSIONS:A single administration of MVs immediately after ischemic AKI could ameliorate renal injury in both the acute and chronic stage, and the anti-inflammatory property of MVs through suppression of CX3CL1 may be a potential mechanism. This establishes a substantial foundation for future research and treatment.


Stem Cell Res Ther


Zou X,Zhang G,Cheng Z,Yin D,Du T,Ju G,Miao S,Liu G,Lu M,Zhu Y




Has Abstract


2014-03-19 00:00:00












  • Automated image analysis detects aging in clinical-grade mesenchymal stromal cell cultures.

    abstract:BACKGROUND:Senescent cells are undesirable in cell therapy products due to reduced therapeutic activity and risk of aberrant cellular effects, and methods for assessing senescence are needed. Early-passage mesenchymal stromal cells (MSCs) are known to be small and spindle-shaped but become enlarged upon cell aging. Ind...

    journal_title:Stem cell research & therapy

    pub_type: 杂志文章


    authors: Oja S,Komulainen P,Penttilä A,Nystedt J,Korhonen M

    更新日期:2018-01-10 00:00:00

  • Protective effect of miRNA-containing extracellular vesicles derived from mesenchymal stromal cells of old rats on renal function in chronic kidney disease.

    abstract:INTRODUCTION:Mesenchymal stromal cells (MSCs) play an important role in the prevention of cell and tissue fibrosis. Senescence may decrease the function of MSCs during recovery from tissue and organ damage. Extracellular vesicles (EVs) released from MSCs contribute to the repair of kidney injury. We explored the influe...

    journal_title:Stem cell research & therapy

    pub_type: 杂志文章


    authors: Wang Y,Guo YF,Fu GP,Guan C,Zhang X,Yang DG,Shi YC

    更新日期:2020-07-08 00:00:00

  • Nanosecond pulsed electric fields enhanced chondrogenic potential of mesenchymal stem cells via JNK/CREB-STAT3 signaling pathway.

    abstract:BACKGROUND:Nanosecond pulsed electric fields (nsPEFs) can produce more significant biological effects than traditional electric fields and have thus attracted rising attention in developing medical applications based on short pulse duration and high field strength, such as effective cancer therapy. However, little is k...

    journal_title:Stem cell research & therapy

    pub_type: 杂志文章


    authors: Ning T,Guo J,Zhang K,Li K,Zhang J,Yang Z,Ge Z

    更新日期:2019-01-24 00:00:00

  • Co-transplantation of mesenchymal stem cells improves spermatogonial stem cell transplantation efficiency in mice.

    abstract:BACKGROUND:Spermatogonial stem cell transplantation (SSCT) could become a fertility restoration tool for childhood cancer survivors. However, since in mice, the colonization efficiency of transplanted spermatogonial stem cells (SSCs) is only 12%, the efficiency of the procedure needs to be improved before clinical impl...

    journal_title:Stem cell research & therapy

    pub_type: 杂志文章


    authors: Kadam P,Ntemou E,Baert Y,Van Laere S,Van Saen D,Goossens E

    更新日期:2018-11-21 00:00:00

  • Myogenic differentiation and reparative activity of stromal cells derived from pericardial adipose in comparison to subcutaneous origin.

    abstract:INTRODUCTION:Adipose tissue-derived stromal cells (ADSCs) are abundant and easy to obtain, but the diversity of differentiation potential from different locations may vary with the developmental origin of their mesenchymal compartment. We therefore aim to compare the myogenic differentiation and reparative activity of ...

    journal_title:Stem cell research & therapy

    pub_type: 杂志文章


    authors: Wang X,Zhang H,Nie L,Xu L,Chen M,Ding Z

    更新日期:2014-08-01 00:00:00

  • EphrinB2 overexpression enhances osteogenic differentiation of dental pulp stem cells partially through ephrinB2-mediated reverse signaling.

    abstract:BACKGROUND:Alveolar bone loss is a frequent occurrence. Dental pulp stem cells (DPSCs) which have invasive accessibility and high osteogenic potential is a promising source for cell-based bone regeneration. EphrinB2 is involved in bone homeostasis and osteogenesis. The aim of this study was to investigate the effect an...

    journal_title:Stem cell research & therapy

    pub_type: 杂志文章


    authors: Wang W,Yuan C,Geng T,Liu Y,Zhu S,Zhang C,Liu Z,Wang P

    更新日期:2020-01-29 00:00:00

  • Epigenetic silencing of KLF2 by long non-coding RNA SNHG1 inhibits periodontal ligament stem cell osteogenesis differentiation.

    abstract:BACKGROUND:Exploring the effects of lncRNA SNHG1 in the process of osteogenic differentiation of periodontal ligament stem cells (PDLSCs) would provide novel therapeutic strategies for tissue regeneration. METHODS:Loss-of-function and gain-of-function assays were induced by lentivirus. The osteogenic differentiation o...

    journal_title:Stem cell research & therapy

    pub_type: 杂志文章


    authors: Li Z,Guo X,Wu S

    更新日期:2020-10-07 00:00:00

  • Transplantation of MHC-mismatched mouse embryonic stem cell-derived thymic epithelial progenitors and MHC-matched bone marrow prevents autoimmune diabetes.

    abstract:BACKGROUND:Type 1 diabetes (T1D) is an autoimmune disease resulting from the destruction of insulin-secreting islet β cells by autoreactive T cells. Non-obese diabetic (NOD) mice are the widely used animal model for human T1D. Autoimmunity in NOD mice is associated with particular major histocompatibility complex (MHC)...

    journal_title:Stem cell research & therapy

    pub_type: 杂志文章


    authors: Su M,Lin Y,He Z,Lai L

    更新日期:2019-08-06 00:00:00

  • 3',4'-Dimethoxyflavone and valproic acid promotes the proliferation of human hematopoietic stem cells.

    abstract:INTRODUCTION:Human hematopoietic stem cells (HSCs) have been clinically used for transplantation and gene and cellular therapy for more than 4 decades. However, this use is limited because of the challenges in the ex vivo culturing of HSCs. The major hurdle is to amplify these cells without losing their self-renewing p...

    journal_title:Stem cell research & therapy

    pub_type: 杂志文章


    authors: Kaur K,Mirlashari MR,Kvalheim G,Kjeldsen-Kragh J

    更新日期:2013-05-24 00:00:00

  • Tackling the physiological barriers for successful mesenchymal stem cell transplantation into the central nervous system.

    abstract::Over the past decade a lot of research has been performed towards the therapeutic use of mesenchymal stem cells (MSCs) in neurodegenerative and neuroinflammatory diseases. MSCs have shown to be beneficial in different preclinical studies of central nervous system (CNS) disorders due to their immunomodulatory propertie...

    journal_title:Stem cell research & therapy

    pub_type: 杂志文章,评审


    authors: De Vocht N,Praet J,Reekmans K,Le Blon D,Hoornaert C,Daans J,Berneman Z,Van der Linden A,Ponsaerts P

    更新日期:2013-08-22 00:00:00

  • Mesenchymal stem cells as a potential therapy for COVID-19.

    abstract::The outbreak of 2019 novel coronavirus disease (COVID-19) worldwide is becoming rapidly a major concern. The number of severe cases has increased dramatically worldwide, while specific treatment options are scarce. The main pathologic features of severe or critical COVID-19 were consistent with acute lung injure (ALI)...

    journal_title:Stem cell research & therapy

    pub_type: 信件


    authors: Liu S,Peng D,Qiu H,Yang K,Fu Z,Zou L

    更新日期:2020-05-04 00:00:00

  • Eliminating senescent chondrogenic progenitor cells enhances chondrogenesis under intermittent hydrostatic pressure for the treatment of OA.

    abstract:BACKGROUND:Osteoarthritis (OA) is a major cause of limb dysfunction, and distraction arthroplasty which generates intermittent hydrostatic pressure (IHP) is an effective approach for OA treatment. However, the result was not always satisfactory and the reasons remained unresolved. Because aging is recognized as an impo...

    journal_title:Stem cell research & therapy

    pub_type: 杂志文章


    authors: Dai H,Chen R,Gui C,Tao T,Ge Y,Zhao X,Qin R,Yao W,Gu S,Jiang Y,Gui J

    更新日期:2020-05-25 00:00:00

  • miR-205-5p inhibits human endometriosis progression by targeting ANGPT2 in endometrial stromal cells.

    abstract:BACKGROUND:miRNA expression profiles in ectopic endometrium (EC) serving as pathophysiologic genetic fingerprints contribute to determining endometriosis progression; however, the underlying molecular mechanisms remain unknown. METHODS:miRNA microarray analysis was used to determine the expression profiling of EC fres...

    journal_title:Stem cell research & therapy

    pub_type: 杂志文章


    authors: Zhou CF,Liu MJ,Wang W,Wu S,Huang YX,Chen GB,Liu LM,Peng DX,Wang XF,Cai XZ,Li XX,Feng WQ,Ma Y

    更新日期:2019-09-23 00:00:00

  • Co-encapsulation of HNF4α overexpressing UMSCs and human primary hepatocytes ameliorates mouse acute liver failure.

    abstract:BACKGROUND:Acute liver failure (ALF) is a complicated condition that is characterized by global hepatocyte death and often requires immediate liver transplantation. However, this therapy is limited by shortage of donor organs. Mesenchymal stem cells (MSCs) and hepatocytes are two attractive sources of cell-based therap...

    journal_title:Stem cell research & therapy

    pub_type: 杂志文章


    authors: Kong D,Xu H,Chen M,Yu Y,Qian Y,Qin T,Tong Y,Xia Q,Hang H

    更新日期:2020-10-23 00:00:00

  • Paracrine stimulation of perinatal lung functional and structural maturation by mesenchymal stem cells.

    abstract:BACKGROUND:Mesenchymal stem cells (MSCs) were shown to harbor therapeutic potential in models of respiratory diseases, such as bronchopulmonary dysplasia (BPD), the most common sequel of preterm birth. In these studies, cells or animals were challenged with hyperoxia or other injury-inducing agents. However, little is ...

    journal_title:Stem cell research & therapy

    pub_type: 杂志文章


    authors: Obendorf J,Fabian C,Thome UH,Laube M

    更新日期:2020-12-09 00:00:00

  • Human adipose-derived stem cells inhibit bioactivity of keloid fibroblasts.

    abstract:BACKGROUND:A keloid is a fibroproliferative disorder occurring in wounds characterized by an exaggerated response to injury. To date, no effective cure has been identified. As multipotent stem cells, human adipose-derived stem cells (ADSCs) may show the possibility for curing diseases such as fibrosis. This study sough...

    journal_title:Stem cell research & therapy

    pub_type: 杂志文章


    authors: Wang X,Ma Y,Gao Z,Yang J

    更新日期:2018-02-21 00:00:00

  • Mesenchymal stem cells inhibit multiple myeloma cells via the Fas/Fas ligand pathway.

    abstract:INTRODUCTION:Cell-based therapy represents a new frontier in the treatment of a wide variety of human diseases traditionally associated with morbidity outcomes, including those involving inflammation, autoimmunity, tissue damage, and cancer. However, the use of mesenchymal stem cells (MSCs) to treat multiple myeloma (M...

    journal_title:Stem cell research & therapy

    pub_type: 杂志文章


    authors: Atsuta I,Liu S,Miura Y,Akiyama K,Chen C,An Y,Shi S,Chen FM

    更新日期:2013-01-01 00:00:00

  • Placental mesenchymal stem cells of fetal and maternal origins demonstrate different therapeutic potentials.

    abstract:INTRODUCTION:Therapeutic potentials of mesenchymal stem cells (MSCs) from different sources have been evaluated in pre-clinical and clinical settings. Although MSCs from different sources share MSC-specific characteristics and functions, inconsistent or controversial results of pre-clinical and clinical applications of...

    journal_title:Stem cell research & therapy

    pub_type: 杂志文章


    authors: Zhu Y,Yang Y,Zhang Y,Hao G,Liu T,Wang L,Yang T,Wang Q,Zhang G,Wei J,Li Y

    更新日期:2014-04-10 00:00:00

  • Development of a novel imaging system for cell therapy in the brain.

    abstract:INTRODUCTION:Stem cells have been evaluated as a potential therapeutic approach for several neurological disorders of the central and peripheral nervous system as well as for traumatic brain and spinal cord injury. Currently, the lack of a reliable and safe method to accurately and non-invasively locate the site of imp...

    journal_title:Stem cell research & therapy

    pub_type: 杂志文章


    authors: Micci MA,Boone DR,Parsley MA,Wei J,Patrikeev I,Motamedi M,Hellmich HL

    更新日期:2015-07-21 00:00:00

  • Transcriptome differences in adipose stromal cells derived from pre- and postmenopausal women.

    abstract:BACKGROUND:As the population ages, an increasing number of postmenopausal women are donors of adipose stromal cells (ASCs) and may benefit from autologous ASC-related treatments. However, the effect of menopausal status on ASCs has not been investigated. METHODS:RNA sequencing data were downloaded, and differentially ...

    journal_title:Stem cell research & therapy

    pub_type: 杂志文章


    authors: Xie Y,Fang B,Liu W,Li G,Huang RL,Zhang L,He J,Zhou S,Liu K,Li Q

    更新日期:2020-02-28 00:00:00

  • Human levator veli palatini muscle: a novel source of mesenchymal stromal cells for use in the rehabilitation of patients with congenital craniofacial malformations.

    abstract:BACKGROUND:Bone reconstruction in congenital craniofacial differences, which affect about 2-3% of newborns, has long been the focus of intensive research in the field of bone tissue engineering. The possibility of using mesenchymal stromal cells in regenerative medicine protocols has opened a new field of investigation...

    journal_title:Stem cell research & therapy

    pub_type: 杂志文章


    authors: Bueno DF,Kabayashi GS,Pinheiro CCG,Tanikawa DYS,Raposo-Amaral CE,Rocha DL,Ferreira JRM,Shibuya Y,Hokugo A,Jarrahy R,ZuK PA,Passos-Bueno MR

    更新日期:2020-11-25 00:00:00

  • Correction to: BM-MSC-derived exosomes alleviate radiation-induced bone loss by restoring the function of recipient BM-MSCs and activating Wnt/β-catenin signaling.

    abstract::The original article [1] contains an error in Fig. 5 whereby sub-Fig. 5c, d & 5e are mistakenly mixed-up. ...

    journal_title:Stem cell research & therapy

    pub_type: 已发布勘误


    authors: Zuo R,Liu M,Wang Y,Li J,Wang W,Wu J,Sun C,Li B,Wang Z,Lan W,Zhang C,Shi C,Zhou Y

    更新日期:2020-01-23 00:00:00

  • Angiogenic potency evaluation of cell therapy candidates by a novel application of the in vitro aortic ring assay.

    abstract:BACKGROUND:Due to limitations of current angiogenesis assays, we aimed to develop a novel application of the rat aortic ring assay to assess the angiogenic potential of mesenchymal stromal cells (MSCs). First-trimester human umbilical cord-derived perivascular cells (FTM HUCPVCs) have multipotent characteristics and pr...

    journal_title:Stem cell research & therapy

    pub_type: 杂志文章


    authors: Iqbal F,Szaraz P,Librach M,Gauthier-Fisher A,Librach CL

    更新日期:2017-08-14 00:00:00

  • An improvised one-step sucrose cushion ultracentrifugation method for exosome isolation from culture supernatants of mesenchymal stem cells.

    abstract:BACKGROUND:Exosomes are nanovesicles (30-120 nm) of endosomal origin. These exosomes contain various functional proteins and RNAs that could be used for therapeutic purposes. Currently, having a standard method for exosome isolation retaining its biological properties with increased yield and purity is a major challeng...

    journal_title:Stem cell research & therapy

    pub_type: 杂志文章


    authors: Gupta S,Rawat S,Arora V,Kottarath SK,Dinda AK,Vaishnav PK,Nayak B,Mohanty S

    更新日期:2018-07-04 00:00:00

  • LAMA2 regulates the fate commitment of mesenchymal stem cells via hedgehog signaling.

    abstract:BACKGROUND:Bone defects are a common clinical condition that has gained an increasing amount of attention in recent years. Causes of bone defect include tumors, inflammation, and fractures. Bone tissue engineering is a novel treatment of bone defect, and human mesenchymal stem cells (hMSCs) are the ideal seed cells for...

    journal_title:Stem cell research & therapy

    pub_type: 杂志文章


    authors: Zhu Y,Zhang X,Gu R,Liu X,Wang S,Xia D,Li Z,Lian X,Zhang P,Liu Y,Zhou Y

    更新日期:2020-03-25 00:00:00

  • Mesenchymal stem cells in cardiac regeneration: a detailed progress report of the last 6 years (2010-2015).

    abstract::Mesenchymal stem cells have been used for cardiovascular regenerative therapy for decades. These cells have been established as one of the potential therapeutic agents, following several tests in animal models and clinical trials. In the process, various sources of mesenchymal stem cells have been identified which hel...

    journal_title:Stem cell research & therapy

    pub_type: 杂志文章,评审


    authors: Singh A,Singh A,Sen D

    更新日期:2016-06-04 00:00:00

  • Efficacy of matrilin-3-primed adipose-derived mesenchymal stem cell spheroids in a rabbit model of disc degeneration.

    abstract:BACKGROUND:Chronic low back pain is a prevalent disability, often caused by intervertebral disc (IVD) degeneration. Mesenchymal stem cell (MSC) therapy could be a safe and feasible option for repairing the degenerated disc. However, for successful translation to the clinic, various challenges need to be overcome includ...

    journal_title:Stem cell research & therapy

    pub_type: 杂志文章


    authors: Muttigi MS,Kim BJ,Kumar H,Park S,Choi UY,Han I,Park H,Lee SH

    更新日期:2020-08-24 00:00:00

  • Hypoxic preconditioning of human urine-derived stem cell-laden small intestinal submucosa enhances wound healing potential.

    abstract:BACKGROUND:Urine-derived stem cells (USCs) are a valuable stem cell source for tissue engineering because they can be harvested non-invasively. Small intestine submucosa (SIS) has been used as scaffolds for soft tissue repair in the clinic. However, the feasibility and efficacy of a combination of USCs and SIS for skin...

    journal_title:Stem cell research & therapy

    pub_type: 杂志文章


    authors: Zhang XR,Huang YZ,Gao HW,Jiang YL,Hu JG,Pi JK,Chen AJ,Zhang Y,Zhou L,Xie HQ

    更新日期:2020-04-06 00:00:00

  • HMGB1-modified mesenchymal stem cells attenuate radiation-induced vascular injury possibly via their high motility and facilitation of endothelial differentiation.

    abstract:BACKGROUND:Vascular injury is one of the most common detrimental effects of cancer radiotherapy on healthy tissues. Since the efficacy of current preventive and therapeutic strategies remains limited, the exploration of new approaches to treat radiation-induced vascular injury (RIV) is on high demands. The use of mesen...

    journal_title:Stem cell research & therapy

    pub_type: 杂志文章


    authors: Tao X,Sun M,Chen M,Ying R,Su W,Zhang J,Xie X,Wei W,Meng X

    更新日期:2019-03-13 00:00:00

  • Extracellular vesicle-encapsulated miR-22-3p from bone marrow mesenchymal stem cell promotes osteogenic differentiation via FTO inhibition.

    abstract:BACKGROUND:Bone marrow mesenchymal stem cells (BMSCs) exhibit the capacity to self-renew and differentiate into multi-lineage cell types, including osteoblasts, which are crucial regulators of fracture healing. Thus, this study aims to investigate the effect of microRNA (miR)-22-3p from BMSC-derived EVs on osteogenic d...

    journal_title:Stem cell research & therapy

    pub_type: 杂志文章


    authors: Zhang X,Wang Y,Zhao H,Han X,Zhao T,Qu P,Li G,Wang W

    更新日期:2020-06-10 00:00:00