Abstract：
BACKGROUND:Stem cells from human exfoliated deciduous teeth (SHED) have been reported to show the in vivo and in vitro hepatic differentiation, SHED-Heps; however, the cholangiogenic potency of SHED-Heps remains unclear. Here, we hypothesized that SHED-Heps contribute to the regeneration of intrahepatic bile duct system in chronic fibrotic liver. METHODS:SHED were induced into SHED-Heps under cytokine stimulation. SHED-Heps were intrasplenically transplanted into chronically CCl4-treated liver fibrosis model mice, followed by the analysis of donor integration and hepatobiliary metabolism in vivo. Immunohistochemical assay was examined for the regeneration of intrahepatic bile duct system in the recipient liver. Furthermore, SHED-Heps were induced under the stimulation of tumor necrosis factor alpha (TNFA). RESULTS:The intrasplenic transplantation of SHED-Heps into CCl4-treated mice showed that donor SHED-Heps behaved as human hepatocyte paraffin 1- and human albumin-expressing hepatocyte-like cells in situ and ameliorated CCl4-induced liver fibrosis. Of interest, the integrated SHED-Heps not only expressed biliary canaliculi ATP-binding cassette transporters including ABCB1, ABCB11, and ABCC2, but also recruited human keratin 19- (KRT19-) and KRT17-positive cells, which are considered donor-derived cholangiocytes, regenerating the intrahepatic bile duct system in the recipient liver. Furthermore, the stimulation of TNFA induced SHED-Heps into KRT7- and SRY-box 9-positive cells. CONCLUSIONS:Collectively, our findings demonstrate that infused SHED-Heps showed cholangiogenic ability under the stimulation of TNFA in CCl4-damaged livers, resulting in the regeneration of biliary canaliculi and interlobular bile ducts in chronic fibrotic liver. Thus, the present findings suggest that SHED-Heps may be a novel source for the treatment of cholangiopathy.
journal_name
Stem Cell Res Therjournal_title
Stem cell research & therapyauthors
Yuniartha R,Yamaza T,Sonoda S,Yoshimaru K,Matsuura T,Yamaza H,Oda Y,Ohga S,Taguchi Tdoi
10.1186/s13287-020-02113-8subject
Has Abstractpub_date
2021-01-13 00:00:00pages
57issue
1issn
1757-6512pii
10.1186/s13287-020-02113-8journal_volume
12pub_type
杂志文章abstract：INTRODUCTION:Nervous system injuries comprise a diverse group of disorders that include traumatic brain injury (TBI). The potential of mesenchymal stem cells (MSCs) to differentiate into neural cell types has aroused hope for the possible development of autologous therapies for central nervous system injury. METHODS:I...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/scrt151
更新日期：2013-01-06 00:00:00
abstract：BACKGROUND:Stem cells from human exfoliated deciduous teeth (SHED) have recently attracted attention as novel multipotential stem cell sources. However, their application is limited due to in vitro replicative senescence. Ectopic expression of telomerase reverse transcriptase (TERT) is a promising strategy for overcomi...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-016-0309-0
更新日期：2016-04-04 00:00:00
abstract：OBJECTIVE:Autograft microskin transplantation has been widely used as a skin graft therapy in full-thickness skin defect. However, skin grafting failure can lead to a pathological delay wound healing due to a poor vascularization bed. Considering the active role of adipose-derived stem cell (ADSC) in promoting angiogen...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-019-1389-4
更新日期：2019-08-30 00:00:00
abstract：:Periostin is a matricellular protein that is reactivated during tissue damage and repair and has been shown to be a critical regulator of multiple biological pathways involved in the repair of tissue after myocardial infarction, peripheral vascular disease, and skin wounds. The tissue repair properties attributed to p...
journal_title：Stem cell research & therapy
pub_type： 评论,杂志文章
doi：10.1186/s13287-015-0215-x
更新日期：2015-11-04 00:00:00
abstract：INTRODUCTION:This is the first study evaluating the interactions of human adipose tissue derived stem cells (ADSCs) and human squamous cell carcinoma cells (SCCs), with regard to a prospective cell-based skin regenerative therapy and a thereby unintended co-localization of ADSCs and SCCs. METHODS:ADSCs were co-culture...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/scrt454
更新日期：2014-05-12 00:00:00
abstract：BACKGROUND:Graft-versus-host disease (GvHD) is the main life-threatening complication of allogeneic hematopoietic stem cell transplantation (HSCT). Thirty to 80% of GvHD patients do not respond to first-line treatment and a second-line treatment is not universally established. Based on their immunomodulatory properties...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-01592-z
更新日期：2020-02-18 00:00:00
abstract：INTRODUCTION:Estrogen plays an important role in the osteogenic differentiation of mesenchymal stem cells, while stem cells from apical papilla (SCAP) can contribute to the formation of dentin/bone-like tissues. To date, the effects of estrogen on the differentiation of SCAP remain unclear. METHODS:SCAP was isolated a...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/scrt515
更新日期：2014-11-17 00:00:00
abstract：BACKGROUND:Bone reconstruction in congenital craniofacial differences, which affect about 2-3% of newborns, has long been the focus of intensive research in the field of bone tissue engineering. The possibility of using mesenchymal stromal cells in regenerative medicine protocols has opened a new field of investigation...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-02017-7
更新日期：2020-11-25 00:00:00
abstract：INTRODUCTION:Mesenchymal stem cells (MSCs) are adult multipotent stem cells that possess regenerative and immunomodulatory properties. They have been widely investigated as therapeutic agents for a variety of disease conditions, including tissue repair, inflammation, autoimmunity, and organ transplantation. Importantly...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-015-0151-9
更新日期：2015-09-21 00:00:00
abstract：BACKGROUND:Vocal fold (VF) scarring, caused by surgery or inflammation, often results in severe voice problems or aphonia. Effective lasting treatment is lacking. Previous in vitro and in vivo animal studies reported positive effects on VF scar resolution with mesenchymal stromal cell (MSC) implantation. The principal ...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-01632-8
更新日期：2020-03-20 00:00:00
abstract：BACKGROUND:Amyotrophic lateral sclerosis (ALS) represents a devastating, progressive, heterogeneous, and the most common motor neuron (MN) disease. To date, no cure has been available for the condition. Studies with transgenic mice have yielded significant results that help us understand the underlying mechanisms of AL...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-018-1048-1
更新日期：2018-11-15 00:00:00
abstract：:Mesenchymal stem cells have been used for cardiovascular regenerative therapy for decades. These cells have been established as one of the potential therapeutic agents, following several tests in animal models and clinical trials. In the process, various sources of mesenchymal stem cells have been identified which hel...
journal_title：Stem cell research & therapy
pub_type： 杂志文章,评审
doi：10.1186/s13287-016-0341-0
更新日期：2016-06-04 00:00:00
abstract：:Temperature is an important microenvironmental factor that functions epigenetically in normal embryonic development. However, the effect of hyperthermia in the stem cells is not fully understood. Oct4 is a tightly regulated master regulator of pluripotency maintenance in stem cells and during early embryonic developme...
journal_title：Stem cell research & therapy
pub_type： 信件
doi：10.1186/s13287-020-01715-6
更新日期：2020-05-24 00:00:00
abstract：BACKGROUND:The avian influenza virus (AIV) can cross species barriers and expand its host range from birds to mammals, even humans. Avian influenza is characterized by pronounced activation of the proinflammatory cytokine cascade, which perpetuates the inflammatory response, leading to persistent systemic inflammatory ...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-016-0395-z
更新日期：2016-10-28 00:00:00
abstract：INTRODUCTION:Adipose tissue-derived stromal cells (ADSCs) are abundant and easy to obtain, but the diversity of differentiation potential from different locations may vary with the developmental origin of their mesenchymal compartment. We therefore aim to compare the myogenic differentiation and reparative activity of ...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/scrt481
更新日期：2014-08-01 00:00:00
abstract：BACKGROUND:Epidermal grafting using cells derived from pluripotent stem cells will change the face of this side of regenerative cutaneous medicine. To date, the safety of the graft would be the major unmet deal in order to implement long-term skin grafting. In this context, experiments on large animals appear unavoidab...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-017-0741-9
更新日期：2017-12-19 00:00:00
abstract：INTRODUCTION:Advances in tendon engineering with mesenchymal stem cells (MSCs) are hindered by a need for cues to direct tenogenesis, and markers to assess tenogenic state. We examined the effects of factors involved in embryonic tendon development on adult MSCs, and compared MSC responses to that of embryonic tendon p...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-015-0043-z
更新日期：2015-05-09 00:00:00
abstract：BACKGROUND:For patients with thromboangiitis obliterans (TAO), revascularization with bypass or angioplasty is frequently not feasible due to the poor outflow of the distal small vessels. We evaluated the long-term results of our experience treating patients with TAO with autologous bone marrow-derived mononuclear cell...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-018-0784-6
更新日期：2018-02-22 00:00:00
abstract：BACKGROUND:Skin is an organ that plays an important role as a physical barrier and has many other complex functions. Skin mimetics may be useful for studying the pathophysiology of diseases in vitro and for repairing lesions in vivo. Cord blood mononuclear cells (CBMCs) have emerged as a potential cell source for regen...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-018-0958-2
更新日期：2018-08-13 00:00:00
abstract：BACKGROUND:Although endothelial progenitor cells (EPCs) contribute to wound repair by promoting neovascularization, the mechanism of EPC-mediated wound healing remains poorly understood due to the lack of pivotal molecular targets of dermal wound repair. METHODS AND RESULTS:We found that genetic targeting of the Lnk g...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-016-0403-3
更新日期：2016-10-28 00:00:00
abstract：BACKGROUND:Parthenogenetic stem cells (PSCs) are a promising source of regenerated cardiomyocytes; however, their application may be limited without a paternal genome. Insulin-like growth factor-II (IGF-II), a paternally expressed growth hormone, is critical in embryonic differentiation. This study investigated whether...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-1575-4
更新日期：2020-02-26 00:00:00
abstract：BACKGROUND:Human embryonic stem cells (hESCs) hold tremendous promise for cell replacement therapies for a range of degenerative diseases. In order to provide cost-effective treatments affordable by public health systems, HLA-matched allogeneic tissue banks of the highest quality clinical-grade hESCs will be required. ...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-017-0561-y
更新日期：2017-06-05 00:00:00
abstract：BACKGROUND:Human adipose-derived stem cells (hASCs) are a subset of mesenchymal stem cells (MSCs); it has been regarded as one of the most promising stem cells. We previously found that fibroblast growth factor-2 (FGF-2) enhanced the proliferation and differentiation of hASC. However, the mechanisms involved in the gro...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-019-1462-z
更新日期：2019-11-27 00:00:00
abstract：INTRODUCTION:Administration of bone marrow-derived cells produces beneficial effects in experimental extrapulmonary acute respiratory distress syndrome (ARDS). However, there are controversies regarding the effects of timing of cell administration and initial insult severity on inflammatory response. We evaluated the e...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/scrt334
更新日期：2013-10-13 00:00:00
abstract：BACKGROUND:Mesenchymal stem cells, including those derived from human adipose tissue (hASCs), are currently being widely investigated for cell therapy. However, when transplanted at the site of injury, the survival and engraftment rates of hASCs are low, mainly due to the harsh microenvironment they encounter, characte...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-01851-z
更新日期：2020-08-03 00:00:00
abstract：BACKGROUND:Patients suffering from osteoporosis show an increased number of adipocytes in their bone marrow, concomitant with a reduction in the pool of human mesenchymal stem cells (hMSCs) that are able to differentiate into osteoblasts, thus leading to suppressed osteogenesis. METHODS:In order to be able to interfer...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-016-0375-3
更新日期：2016-08-26 00:00:00
abstract：BACKGROUND:C-kit-positive cardiac stem cells (CSCs) have been shown to be a promising candidate treatment for myocardial infarction and heart failure. Insulin-like growth factor (IGF)-1 is an anabolic growth hormone that regulates cellular proliferation, differentiation, senescence, and death in various tissues. Althou...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-017-0762-4
更新日期：2018-02-21 00:00:00
abstract：BACKGROUND:Mesenchymal stromal cells (MSCs) have been largely investigated, in the past decade, as potential therapeutic strategies for various acute and chronic pathological conditions. MSCs isolated from different sources, such as bone marrow (BM), umbilical cord tissue (UCT) and adipose tissue (AT), share many biolo...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-016-0383-3
更新日期：2016-08-25 00:00:00
abstract：BACKGROUND:In recent years, adipose-derived stromal cells (ASCs) have been heavily studied for soft tissue regeneration, augmentation, and dermal wound healing. METHODS:In this review, we investigated the trends in injectable scaffolds for ASC delivery in the dermis, and injectable or implantable scaffolds for ASC del...
journal_title：Stem cell research & therapy
pub_type： 杂志文章,评审
doi：10.1186/s13287-020-02132-5
更新日期：2021-01-19 00:00:00
abstract：:The efficacy of tendon-derived stem cells (TDSCs) for the promotion of tendon and tendon-bone junction repair has been reported in animal studies. Modulation of the tendon stem cell niche in vivo has also been reported to influence tendon structure. There is a need to have specific and reliable markers that can define...
journal_title：Stem cell research & therapy
pub_type： 杂志文章,评审
doi：10.1186/s13287-015-0097-y
更新日期：2015-06-02 00:00:00
