Abstract:
INTRODUCTION:Impaction allograft with cement is a common technique used in revision hip surgeries for the last 20 years. However, its clinical results are inconsistent. Recent studies have shown that mesenchymal stem cells (MSCs) seeded onto allograft can enhance bone formation. This in vitro study investigates whether the increase in temperature related to the polymerisation of bone cement will affect the viability of human MSCs. METHODS:The viability of human MSCs was measured after incubating them at temperatures of 38°C, 48°C and 58°C; durations 45 seconds, 80 seconds and 150 seconds. A control group was kept at 37°C and 5% carbon dioxide for the duration of the investigation (7 days). During the course of the study the human MSCs were analysed for cell metabolic activity using the alamarBlue™ assay, cell viability using both Trypan Blue dye exclusion and calcein staining under fluorescent microscopy, and necrosis and apoptosis using Annexin V and propidium iodide for flow cytometric analysis. A one-way analysis of variance with a priori Dunnett’s test was used to indicate the differences between the treatment groups, when analysed against the control. This identified conditions with a significant difference in cell metabolic activity (alamarBlue™) and cell viability (Trypan Blue). RESULTS:Results showed that cell metabolism was not severely affected up to 48°C/150 seconds, while cells in the 58°C group died. Similar results were shown using Trypan Blue and calcein analysis for cell viability. No significant difference in apoptosis and necrosis of the cells was observed when human MSCs treated at 48°C/150 seconds were compared with the control group. CONCLUSIONS:The study suggests that human MSCs seeded onto allograft can be exposed to temperatures up to 48°C for 150 seconds. Exposure to this temperature for this time period is unlikely to occur during impaction allograft surgery when cement is used. Therefore, in many situations, the addition of human MSCs to cemented impaction grafting may be carried out without detrimental effects to the cells. Furthermore, previous studies have shown that this can enhance new bone formation and repair the defects in revision situations.
journal_name
Stem Cell Res Therjournal_title
Stem cell research & therapyauthors
Reissis Y,García-Gareta E,Korda M,Blunn GW,Hua Jdoi
10.1186/scrt350subject
Has Abstractpub_date
2013-01-01 00:00:00pages
139issue
6issn
1757-6512pii
scrt350journal_volume
4pub_type
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journal_title:Stem cell research & therapy
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pub_type: 杂志文章,评审
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journal_title:Stem cell research & therapy
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更新日期:2020-10-15 00:00:00
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journal_title:Stem cell research & therapy
pub_type: 杂志文章
doi:10.1186/scrt292
更新日期:2013-08-08 00:00:00
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journal_title:Stem cell research & therapy
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journal_title:Stem cell research & therapy
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更新日期:2014-08-21 00:00:00
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journal_title:Stem cell research & therapy
pub_type: 杂志文章
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更新日期:2017-11-13 00:00:00
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journal_title:Stem cell research & therapy
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journal_title:Stem cell research & therapy
pub_type: 杂志文章
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journal_title:Stem cell research & therapy
pub_type: 杂志文章
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更新日期:2011-11-14 00:00:00
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journal_title:Stem cell research & therapy
pub_type: 杂志文章
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更新日期:2014-08-01 00:00:00
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journal_title:Stem cell research & therapy
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更新日期:2013-04-23 00:00:00
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更新日期:2014-08-13 00:00:00
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