Abstract：
INTRODUCTION:Stem cells have been evaluated as a potential therapeutic approach for several neurological disorders of the central and peripheral nervous system as well as for traumatic brain and spinal cord injury. Currently, the lack of a reliable and safe method to accurately and non-invasively locate the site of implantation and track the migration of stem cells in vivo hampers the development of stem cell therapy and its clinical application. In this report, we present data that demonstrate the feasibility of using the human sodium iodide symporter (hNIS) as a reporter gene for tracking neural stem cells (NSCs) after transplantation in the brain by using single-photon emission tomography/computed tomography (SPECT/CT) imaging. METHODS:NSCs were isolated from the hippocampus of adult rats (Hipp-NSCs) and transduced with a lentiviral vector containing the hNIS gene. Hipp-NSCs expressing the hNIS (NIS-Hipp-NSCs) were characterized in vitro and in vivo after transplantation in the rat brain and imaged by using technetium-99m ((99m)Tc) and a small rodent SPECT/CT apparatus. Comparisons were made between Hipp-NSCs and NIS-Hipp-NSCs, and statistical analysis was performed by using two-tailed Student's t test. RESULTS:Our results show that the expression of the hNIS allows the repeated visualization of NSCs in vivo in the brain by using SPECT/CT imaging and does not affect the ability of Hipp-NSCs to generate neuronal and glial cells in vitro and in vivo. CONCLUSIONS:These data support the use of the hNIS as a reporter gene for non-invasive imaging of NSCs in the brain. The repeated, non-invasive tracking of implanted cells will accelerate the development of effective stem cell therapies for traumatic brain injury and other types of central nervous system injury.
journal_name
Stem Cell Res Therjournal_title
Stem cell research & therapyauthors
Micci MA,Boone DR,Parsley MA,Wei J,Patrikeev I,Motamedi M,Hellmich HLdoi
10.1186/s13287-015-0129-7subject
Has Abstractpub_date
2015-07-21 00:00:00pages
131issn
1757-6512pii
10.1186/s13287-015-0129-7journal_volume
6pub_type
杂志文章abstract：BACKGROUND:Nitric oxide (NO) plays a role in a number of physiological processes including stem cell differentiation and osteogenesis. Endothelial nitric oxide synthase (eNOS), one of three NO-producing enzymes, is located in a close conformation with the caveolin-1 (CAV-1WT) membrane protein which is inhibitory to NO ...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-016-0442-9
更新日期：2016-12-07 00:00:00
abstract：BACKGROUND:Autologous epidermal basal cell suspension therapy has been proven to be one of the most effective treatments for full-thickness wounds. However, we found there remain obvious defects that significantly confined the utilization and function of the epidermal basal cells (EBCs), especially the epidermal stem c...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-019-1236-7
更新日期：2019-09-11 00:00:00
abstract：BACKGROUND:Dystrophic epidermolysis bullosa (DEB), a rare genodermatosis, is characterized by the formation of intra-epidermal blistering and the development of chronic nonhealing skin wounds. Recently, attempts have been made to develop cell-based therapies for this currently intractable disorder. The molecular mechan...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-016-0388-y
更新日期：2016-08-27 00:00:00
abstract：:Periostin is a matricellular protein that is reactivated during tissue damage and repair and has been shown to be a critical regulator of multiple biological pathways involved in the repair of tissue after myocardial infarction, peripheral vascular disease, and skin wounds. The tissue repair properties attributed to p...
journal_title：Stem cell research & therapy
pub_type： 评论,杂志文章
doi：10.1186/s13287-015-0215-x
更新日期：2015-11-04 00:00:00
abstract：OBJECTIVES:Mesenchymal stem cells (MSCs) have been intensively investigated as to their therapeutic potentials. However, the full chemical-defined medium supporting the isolation and expansion of human MSCs has not been developed yet. MATERIALS AND METHODS:Here, we developed the full chemical-defined medium, NBVbe med...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-01641-7
更新日期：2020-03-19 00:00:00
abstract：BACKGROUND:Exosomes are nanovesicles (30-120 nm) of endosomal origin. These exosomes contain various functional proteins and RNAs that could be used for therapeutic purposes. Currently, having a standard method for exosome isolation retaining its biological properties with increased yield and purity is a major challeng...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-018-0923-0
更新日期：2018-07-04 00:00:00
abstract：BACKGROUND:Lung cancer stem cells have the ability to self-renew and are resistant to conventional chemotherapy. MicroRNAs (miRNAs) regulate and control the expression and function of many target genes; therefore, miRNA disorders are involved in the pathogenesis of human diseases, such as cancer. However, the effects o...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-018-1072-1
更新日期：2018-11-23 00:00:00
abstract：BACKGROUND:Spermatogonial stem cell transplantation (SSCT) could become a fertility restoration tool for childhood cancer survivors. However, since in mice, the colonization efficiency of transplanted spermatogonial stem cells (SSCs) is only 12%, the efficiency of the procedure needs to be improved before clinical impl...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-018-1065-0
更新日期：2018-11-21 00:00:00
abstract：BACKGROUND:Nicotinamide adenine dinucleotide (NAD+) is a critical molecule involved in various biological functions. Poly (ADP-ribose) polymerase 1 (PARP1) and sirtuin 1 (SIRT1) affect cellular NAD+ levels and play essential roles in regulating metabolism. However, there has been little research on the effects of PARP1...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-018-0961-7
更新日期：2018-08-23 00:00:00
abstract：BACKGROUND:Complex immunophenotypic repertoires defining discrete adipose-derived stem cell (ASC) subpopulations may hold a key toward identifying predictors of clinical utility. To this end, we sorted out of the freshly established ASCs four subpopulations (SPs) according to a specific pattern of co-expression of six ...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-016-0435-8
更新日期：2016-12-01 00:00:00
abstract：BACKGROUND:Endothelial cells have been shown to mediate angiogenesis in ischemic injury sites and contribute to the repair of damaged tissues. However, the treatment of ischemic disease requires a significant number of endothelial cells, which are difficult to isolate from patients. Embryonic stem cells have been consi...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-018-0945-7
更新日期：2018-07-18 00:00:00
abstract：INTRODUCTION:Idiopathic pulmonary fibrosis is a progressive diffuse parenchymal lung disorder of unknown etiology. Mesenchymal stem cell (MSC)-based therapy is a novel approach with great therapeutic potential for the treatment of lung diseases. Despite demonstration of MSC grafting, the populations of engrafted MSCs h...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-015-0081-6
更新日期：2015-05-20 00:00:00
abstract：BACKGROUND:In the brain of adult mammals, neural stem cells persist in the subventricular zone of the lateral ventricle and the subgranular zone of the dentate gyrus, which are specialized niches with proliferative capacity. Most neural stem cells are in a quiescent state, but in response to extrinsic stimuli, they can...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-02119-2
更新日期：2021-01-09 00:00:00
abstract：BACKGROUND:Intervertebral disc (IVD) degeneration is characterized by an early decrease in cellularity of the nucleus pulposus (NP) region, and associated extracellular matrix changes, reduced hydration, and progressive degeneration. Cell-based IVD therapy has emerged as an area of great interest, with studies reportin...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-018-0797-1
更新日期：2018-03-09 00:00:00
abstract：INTRODUCTION:Mesenchymal stromal cells (MSCs) play an important role in the prevention of cell and tissue fibrosis. Senescence may decrease the function of MSCs during recovery from tissue and organ damage. Extracellular vesicles (EVs) released from MSCs contribute to the repair of kidney injury. We explored the influe...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-01792-7
更新日期：2020-07-08 00:00:00
abstract：BACKGROUND:Bone marrow mesenchymal stem cells (BMMSCs) are suitable cell sources for dental pulp regeneration, but the mechanism of BMMSCs differentiation into odontogenic lineage remains unknown. The aim of the present study was to reveal the role of magnesium transporter protein 1 (MagT1) and MAPK pathways in the odo...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-019-1148-6
更新日期：2019-01-31 00:00:00
abstract：BACKGROUND:The therapeutic effects of adipose-derived mesenchymal stem cell (ADSC) transplantation have been demonstrated in several models of central nervous system (CNS) injury and are thought to involve the modulation of the inflammatory response. However, the exact underlying molecular mechanism is poorly understoo...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-01724-5
更新日期：2020-06-03 00:00:00
abstract：:Mesenchymal stem cells have been used for cardiovascular regenerative therapy for decades. These cells have been established as one of the potential therapeutic agents, following several tests in animal models and clinical trials. In the process, various sources of mesenchymal stem cells have been identified which hel...
journal_title：Stem cell research & therapy
pub_type： 杂志文章,评审
doi：10.1186/s13287-016-0341-0
更新日期：2016-06-04 00:00:00
abstract：:Temperature is an important microenvironmental factor that functions epigenetically in normal embryonic development. However, the effect of hyperthermia in the stem cells is not fully understood. Oct4 is a tightly regulated master regulator of pluripotency maintenance in stem cells and during early embryonic developme...
journal_title：Stem cell research & therapy
pub_type： 信件
doi：10.1186/s13287-020-01715-6
更新日期：2020-05-24 00:00:00
abstract：BACKGROUND:MEG3 downregulated the expression in several tumors and inhibits human tumorigenesis. But so far, the mechanism of MEG3 in tumorigenesis is still unclear. METHODS:In gene infection, cellular and molecular technologies and tumorigenesis test in vitro and in vivo were performed, respectively. RESULTS:Our res...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-02036-4
更新日期：2020-11-30 00:00:00
abstract：INTRODUCTION:Studies have demonstrated that mesenchymal stromal cells (MSCs) could reverse acute and chronic kidney injury by a paracrine or endocrine mechanism, and microvesicles (MVs) have been regarded as a crucial means of intercellular communication. In the current study, we focused on the therapeutic effects of h...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/scrt428
更新日期：2014-03-19 00:00:00
abstract：BACKGROUND:Treatment-induced cardiotoxicity is a leading noncancer-related cause of acute and late onset morbidity and mortality in cancer patients on antineoplastic drugs such as melphalan-increasing clinical case reports have documented that it could induce cardiotoxicity including severe arrhythmias and heart failur...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-01984-1
更新日期：2020-11-05 00:00:00
abstract：BACKGROUND:The transfection of human mesenchymal stem cells (hMSCs) with the hyperpolarization-activated cyclic nucleotide-gated ion channel 2 (HCN2) gene has been demonstrated to provide biological pacing in dogs with complete heart block. The mechanism appears to be the generation of the ion current (If) by the HCN2-...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-016-0326-z
更新日期：2016-04-30 00:00:00
abstract：BACKGROUND:C-kit-positive cardiac stem cells (CSCs) have been shown to be a promising candidate treatment for myocardial infarction and heart failure. Insulin-like growth factor (IGF)-1 is an anabolic growth hormone that regulates cellular proliferation, differentiation, senescence, and death in various tissues. Althou...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-017-0762-4
更新日期：2018-02-21 00:00:00
abstract：BACKGROUND:The avian influenza virus (AIV) can cross species barriers and expand its host range from birds to mammals, even humans. Avian influenza is characterized by pronounced activation of the proinflammatory cytokine cascade, which perpetuates the inflammatory response, leading to persistent systemic inflammatory ...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-016-0395-z
更新日期：2016-10-28 00:00:00
abstract：BACKGROUND:Bone reconstruction in congenital craniofacial differences, which affect about 2-3% of newborns, has long been the focus of intensive research in the field of bone tissue engineering. The possibility of using mesenchymal stromal cells in regenerative medicine protocols has opened a new field of investigation...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-02017-7
更新日期：2020-11-25 00:00:00
abstract：BACKGROUND:Many studies have shown that long noncoding RNAs (lncRNAs) are closely related to the stimulation of osteogenic differentiation of adipose-derived stem cells (ADSCs) and the prevention of osteoporosis. Current research aimed to investigate the novel lncRNA and explored the function and molecular mechanism of...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-01754-z
更新日期：2020-06-17 00:00:00
abstract：BACKGROUND:To investigate the therapeutic effect of intercellular adhesion molecule (ICAM)-1-modified mesenchymal stem cells (MSCs) in a mouse model of inflammatory bowel disease (IBD) induced by dextran sulfate sodium. METHODS:Primary MSCs and ICAM-1-overexpressing MSCs (C3 cells) were generated in vitro. The IBD mou...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-019-1384-9
更新日期：2019-08-23 00:00:00
abstract：BACKGROUND:Many factors may influence the efficacy of cell therapy for intrinsic urinary sphincter deficiency (ISD), including the route of administration of the cells and the condition of the sphincter. The goal of this study was to compare local versus intravenous administration of autologous skeletal muscle precurso...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-016-0411-3
更新日期：2016-10-07 00:00:00
abstract：BACKGROUND:Senescent cells are undesirable in cell therapy products due to reduced therapeutic activity and risk of aberrant cellular effects, and methods for assessing senescence are needed. Early-passage mesenchymal stromal cells (MSCs) are known to be small and spindle-shaped but become enlarged upon cell aging. Ind...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-017-0740-x
更新日期：2018-01-10 00:00:00
