Adipose tissue-derived extracellular fraction characterization: biological and clinical considerations in regenerative medicine.

abstract：BACKGROUND:Transplantation of endothelial progenitor cells (EPCs)/endothelial cells (ECs) has been used for the treatment of ischemic diseases and hemophilia A, due to their great capacity for producing factor VIII and for repairing vascular damage. We established an effective approach to stimulate the expansion and di...

journal_title：Stem cell research & therapy

authors： Qin M,Guan X,Wang H,Zhang Y,Shen B,Zhang Q,Dai W,Ma Y,Jiang Y

更新日期：2017-02-07 00:00:00

abstract：:The discovery of induced pluripotent stem cells (iPSCs) in 2006 was a major breakthrough for regenerative medicine. The establishment of patient-specific iPSCs has created the opportunity to model diseases in culture systems, with the potential to rapidly advance the drug discovery field. Current methods of drug disco...

journal_title：Stem cell research & therapy

authors： Guo Z,Higgins CA,Gillette BM,Itoh M,Umegaki N,Gledhill K,Sia SK,Christiano AM

更新日期：2013-01-01 00:00:00

abstract：BACKGROUND:Osteoarthritis (OA) is a major cause of limb dysfunction, and distraction arthroplasty which generates intermittent hydrostatic pressure (IHP) is an effective approach for OA treatment. However, the result was not always satisfactory and the reasons remained unresolved. Because aging is recognized as an impo...

journal_title：Stem cell research & therapy

authors： Dai H,Chen R,Gui C,Tao T,Ge Y,Zhao X,Qin R,Yao W,Gu S,Jiang Y,Gui J

更新日期：2020-05-25 00:00:00

abstract：BACKGROUND:Nanosecond pulsed electric fields (nsPEFs) can produce more significant biological effects than traditional electric fields and have thus attracted rising attention in developing medical applications based on short pulse duration and high field strength, such as effective cancer therapy. However, little is k...

journal_title：Stem cell research & therapy

authors： Ning T,Guo J,Zhang K,Li K,Zhang J,Yang Z,Ge Z

更新日期：2019-01-24 00:00:00

abstract：BACKGROUND:Senescent cells are undesirable in cell therapy products due to reduced therapeutic activity and risk of aberrant cellular effects, and methods for assessing senescence are needed. Early-passage mesenchymal stromal cells (MSCs) are known to be small and spindle-shaped but become enlarged upon cell aging. Ind...

journal_title：Stem cell research & therapy

authors： Oja S,Komulainen P,Penttilä A,Nystedt J,Korhonen M

更新日期：2018-01-10 00:00:00

abstract：BACKGROUND:Efficient and sustained hematopoietic recovery after hematopoietic stem cell or bone marrow transplantation is supported by paracrine signaling from specific subpopulations of mesenchymal stromal cells (MSCs). Here, we considered whether in vitro mechanopriming of human MSCs could be administered to predicti...

journal_title：Stem cell research & therapy

authors： Liu FD,Tam K,Pishesha N,Poon Z,Van Vliet KJ

更新日期：2018-10-24 00:00:00

abstract：BACKGROUND:Induced pluripotent stem cells (iPSCs) exhibit limitless pluripotent plasticity and proliferation capability to provide an abundant cell source for tissue regenerative medicine. Thus, inducing iPSCs toward a specific differentiation direction is an important scientific question. Traditionally, iPSCs have bee...

journal_title：Stem cell research & therapy

authors： Hu Y,Chen L,Gao Y,Cheng P,Yang L,Wu C,Jie Q

更新日期：2020-02-21 00:00:00

abstract：BACKGROUND:Multiple sclerosis is a demyelinating disease mostly of autoimmune origin that affects and damages the central nervous system, leading to a disabling condition. The aim of the present study was to investigate whether administration of mesenchymal stem cells from human periodontal ligament (hPDLSCs) could ame...

journal_title：Stem cell research & therapy

authors： Trubiani O,Giacoppo S,Ballerini P,Diomede F,Piattelli A,Bramanti P,Mazzon E

更新日期：2016-01-04 00:00:00

abstract：BACKGROUND:This study explored the details of the immune response after autologous hematopoietic stem cell transplantation (AHSCT) treatment in type 1 diabetes mellitus. METHODS:Peripheral blood mononuclear cells (PBMCs) from 18 patients with type 1 diabetes mellitus were taken at baseline and 12 months after AHSCT or...

journal_title：Stem cell research & therapy

authors： Ye L,Li L,Wan B,Yang M,Hong J,Gu W,Wang W,Ning G

更新日期：2017-04-18 00:00:00

abstract：BACKGROUND:Expansion protocols aim at both increasing the number of umbilical cord blood (UCB) hematopoietic stem cells and progenitor cells (HSPCs) and reducing the period of neutropenia in UCB HSPC graft. Because glycosaminoglycans (GAGs) are known to be important components of the hematopoietic niche and to modulate...

journal_title：Stem cell research & therapy

authors： Faivre L,Parietti V,Siñeriz F,Chantepie S,Gilbert-Sirieix M,Albanese P,Larghero J,Vanneaux V

更新日期：2016-01-07 00:00:00

abstract：:Adipogenesis is essential in in vitro experimentation to assess differentiation capability of stem cells, and therefore, its accurate measurement is important. Quantitative analysis of adipogenic levels, however, is challenging and often susceptible to errors due to non-specific reading or manual estimation by observe...

journal_title：Stem cell research & therapy

authors： Yuan C,Chakraborty S,Chitta KK,Subramanian S,Lim TE,Han W,Bhanu Prakash KN,Sugii S

更新日期：2019-01-22 00:00:00

abstract：BACKGROUND:Inner ear supporting cells (SCs) in the neonatal mouse cochlea are a potential source for hair cell (HC) regeneration, but several studies have shown that the regeneration ability of SCs decreases dramatically as mice age and that lost HCs cannot be regenerated in adult mice. To better understand how SCs mig...

journal_title：Stem cell research & therapy

authors： Cheng C,Wang Y,Guo L,Lu X,Zhu W,Muhammad W,Zhang L,Lu L,Gao J,Tang M,Chen F,Gao X,Li H,Chai R

更新日期：2019-12-02 00:00:00

abstract：BACKGROUND:Considerable progress has been made in converting human pluripotent stem cells (hPSCs) into cortical neurons for disease modeling and regenerative medicine. However, these procedures are hard to provide sufficient cells for their applications. Using a combination of small-molecules and growth factors, we pre...

journal_title：Stem cell research & therapy

authors： Zhao S,Duan K,Ai Z,Niu B,Chen Y,Kong R,Li T

更新日期：2020-10-02 00:00:00

abstract：INTRODUCTION:Estrogen plays an important role in the osteogenic differentiation of mesenchymal stem cells, while stem cells from apical papilla (SCAP) can contribute to the formation of dentin/bone-like tissues. To date, the effects of estrogen on the differentiation of SCAP remain unclear. METHODS:SCAP was isolated a...

journal_title：Stem cell research & therapy

authors： Li Y,Yan M,Wang Z,Zheng Y,Li J,Ma S,Liu G,Yu J

更新日期：2014-11-17 00:00:00

abstract：INTRODUCTION:This is the first study evaluating the interactions of human adipose tissue derived stem cells (ADSCs) and human squamous cell carcinoma cells (SCCs), with regard to a prospective cell-based skin regenerative therapy and a thereby unintended co-localization of ADSCs and SCCs. METHODS:ADSCs were co-culture...

journal_title：Stem cell research & therapy

authors： Koellensperger E,Gramley F,Preisner F,Leimer U,Germann G,Dexheimer V

更新日期：2014-05-12 00:00:00

abstract：BACKGROUND:Stem cells from human exfoliated deciduous teeth (SHED) have been reported to show the in vivo and in vitro hepatic differentiation, SHED-Heps; however, the cholangiogenic potency of SHED-Heps remains unclear. Here, we hypothesized that SHED-Heps contribute to the regeneration of intrahepatic bile duct syste...

journal_title：Stem cell research & therapy

authors： Yuniartha R,Yamaza T,Sonoda S,Yoshimaru K,Matsuura T,Yamaza H,Oda Y,Ohga S,Taguchi T

更新日期：2021-01-13 00:00:00

abstract：INTRODUCTION:Mesenchymal stromal cells (MSCs) play an important role in the prevention of cell and tissue fibrosis. Senescence may decrease the function of MSCs during recovery from tissue and organ damage. Extracellular vesicles (EVs) released from MSCs contribute to the repair of kidney injury. We explored the influe...

journal_title：Stem cell research & therapy

authors： Wang Y,Guo YF,Fu GP,Guan C,Zhang X,Yang DG,Shi YC

更新日期：2020-07-08 00:00:00

abstract：BACKGROUND:Due to limitations of current angiogenesis assays, we aimed to develop a novel application of the rat aortic ring assay to assess the angiogenic potential of mesenchymal stromal cells (MSCs). First-trimester human umbilical cord-derived perivascular cells (FTM HUCPVCs) have multipotent characteristics and pr...

journal_title：Stem cell research & therapy

authors： Iqbal F,Szaraz P,Librach M,Gauthier-Fisher A,Librach CL

更新日期：2017-08-14 00:00:00

abstract：BACKGROUND:A keloid is a fibroproliferative disorder occurring in wounds characterized by an exaggerated response to injury. To date, no effective cure has been identified. As multipotent stem cells, human adipose-derived stem cells (ADSCs) may show the possibility for curing diseases such as fibrosis. This study sough...

journal_title：Stem cell research & therapy

authors： Wang X,Ma Y,Gao Z,Yang J

更新日期：2018-02-21 00:00:00

abstract：BACKGROUND:Stress urinary incontinence (SUI) is a common and bothersome condition. Invasive surgery will always be considered after conservative treatment fails, but the rates of postoperative complications and long-term recurrence are high. Thus, a new treatment strategy is still needed. In recent years, bone marrow m...

journal_title：Stem cell research & therapy

authors： Zhang H,Huang J,Liu J,Li Y,Gao Y

更新日期：2020-07-16 00:00:00

abstract：BACKGROUND:Neural stem cells for the treatment of spinal cord injury (SCI) are of particular interest for future therapeutic use. However, until now, stem cell therapies are often limited due to the inhibitory environment following the injury. Therefore, in this study, we aimed at testing a combinatorial approach with ...

journal_title：Stem cell research & therapy

authors： Butenschön J,Zimmermann T,Schmarowski N,Nitsch R,Fackelmeier B,Friedemann K,Radyushkin K,Baumgart J,Lutz B,Leschik J

更新日期：2016-01-13 00:00:00

abstract：BACKGROUND:Mesenchymal stem cell (MSC)-based cartilage tissue regeneration is a treatment with great potential. How to enhance the MSC chondrogenic differentiation is a key issue involved in cartilage formation. In the present study, we seek to expound the phenotypes and mechanisms of DLX5 in chondrogenic differentiati...

journal_title：Stem cell research & therapy

authors： Yang H,Cao Y,Zhang J,Liang Y,Su X,Zhang C,Liu H,Han X,Ge L,Fan Z

更新日期：2020-07-06 00:00:00

abstract：BACKGROUND:WNT and TGFβ signaling pathways play critical regulatory roles in cardiomyocyte fate determination and differentiation. MiRNAs are also known to regulate different biological processes and signaling pathways. Here, we intended to find candidate miRNAs that are involved in cardiac differentiation through regu...

journal_title：Stem cell research & therapy

authors： Kay M,Soltani BM,Aghdaei FH,Ansari H,Baharvand H

更新日期：2019-06-27 00:00:00

abstract：BACKGROUND:Non-small cell lung cancer (NSCLC) is the second most prevalent cause of cancer-related fatality. Long non-coding RNAs (lncRNAs) have been observed to exercise functions in NSCLC. Here, the current study aimed to explore the potential mechanism of lncRNA MBNL1-AS1 in NSCLC. METHODS:Microarray analysis was p...

journal_title：Stem cell research & therapy

authors： Li P,Xing W,Xu J,Yuan D,Liang G,Liu B,Ma H

更新日期：2019-05-21 00:00:00

abstract：BACKGROUND:Hematopoietic stem cell (HSC) transplantation has been widely applied to the treatment of malignant blood diseases. However, limited number of functional HSCs hinders successful transplantation. The purpose of our current study is to develop a new and cost-efficient medium formulation that could greatly enha...

journal_title：Stem cell research & therapy

authors： Zhang Y,Shen B,Guan X,Qin M,Ren Z,Ma Y,Dai W,Ding X,Jiang Y

更新日期：2019-06-13 00:00:00

abstract：INTRODUCTION:Mesenchymal stem cells (MSCs) are multipotent and have been derived from various tissues. Although MSCs share many basic features, they often display subtle tissue specific differences. We previously demonstrated that bone marrow (BM) MSCs frequently become polyploid in culture. This tendency was mediated ...

journal_title：Stem cell research & therapy

authors： Ravid O,Shoshani O,Sela M,Weinstock A,Sadan TW,Gur E,Zipori D,Shani N

更新日期：2014-12-17 00:00:00

abstract：:Extracellular vesicles (EVs) contain proteins, microRNAs, mRNAs, long non-coding RNAs, and phospholipids, and are a novel mechanism of intercellular communication. It has been proposed that the immunomodulatory and regenerative effects of mesenchymal stem/stromal cells (MSCs) are mainly mediated by soluble paracrine f...

journal_title：Stem cell research & therapy

authors： Qiu G,Zheng G,Ge M,Wang J,Huang R,Shu Q,Xu J

更新日期：2019-11-28 00:00:00

abstract：BACKGROUND:Vascular injury is one of the most common detrimental effects of cancer radiotherapy on healthy tissues. Since the efficacy of current preventive and therapeutic strategies remains limited, the exploration of new approaches to treat radiation-induced vascular injury (RIV) is on high demands. The use of mesen...

journal_title：Stem cell research & therapy

authors： Tao X,Sun M,Chen M,Ying R,Su W,Zhang J,Xie X,Wei W,Meng X

更新日期：2019-03-13 00:00:00

abstract：BACKGROUND:The transfection of human mesenchymal stem cells (hMSCs) with the hyperpolarization-activated cyclic nucleotide-gated ion channel 2 (HCN2) gene has been demonstrated to provide biological pacing in dogs with complete heart block. The mechanism appears to be the generation of the ion current (If) by the HCN2-...

journal_title：Stem cell research & therapy

authors： Bruzauskaite I,Bironaite D,Bagdonas E,Skeberdis VA,Denkovskij J,Tamulevicius T,Uvarovas V,Bernotiene E

更新日期：2016-04-30 00:00:00

abstract：BACKGROUND:Endothelial cells have been shown to mediate angiogenesis in ischemic injury sites and contribute to the repair of damaged tissues. However, the treatment of ischemic disease requires a significant number of endothelial cells, which are difficult to isolate from patients. Embryonic stem cells have been consi...

journal_title：Stem cell research & therapy

authors： Park JK,Lee TW,Do EK,Moon HJ,Kim JH

更新日期：2018-07-18 00:00:00