Abstract：
BACKGROUND:Diagnostic procedures for the diagnosis of infection with the nematode parasite Onchocerca volvulus are currently based on the microscopic detection of microfilariae in skin biopsies. Alternative approaches based on amplification of parasitic DNA in these skin biopsies are currently being explored. Mostly this is based on the detection of the O-150 repeat sequence using PCR based techniques. METHODS:An isothermal, loop-mediated amplification method has been designed using the mitochondrial O. volvulus cox1 gene as a target. RESULTS:Analysis of dilution series of synthetic DNA containing the targeted sequence show a non-linear dose-response curve, as is usually the case for isothermal amplification methods. Evaluation of cross-reactivity with the heterologous sequence from the closely related parasites Wuchereria bancrofti, Loa loa and Brugia malayi demonstrated strong specificity, as none of these sequences was amplified. The assay however amplified both O. volvulus and O. ochengi DNA, but with a different melting point that can be used to discriminate between the species. Evaluation of this assay in a set of skin snip biopsies collected in an endemic area in Ghana showed a high correlation with O-150 qPCR and also demonstrated a similar sensitivity. Compared to qPCR, LAMP had a sensitivity of 88.2% and a specificity of 99.2%. CONCLUSIONS:We have developed a sensitive and specific loop-mediated amplification method for detection of O. volvulus DNA in skin biopsies that is capable of providing results within 30 min.
journal_name
Parasit Vectorsjournal_title
Parasites & vectorsauthors
Lagatie O,Merino M,Batsa Debrah L,Debrah AY,Stuyver LJdoi
10.1186/s13071-016-1913-7subject
Has Abstractpub_date
2016-12-01 00:00:00pages
624issue
1issn
1756-3305pii
10.1186/s13071-016-1913-7journal_volume
9pub_type
杂志文章abstract：BACKGROUND:Blackflies (Diptera: Simuliidae) are known as effective vectors of human and animal pathogens, worldwide. We have already indicated that some individuals in the Simulium turgaicum complex are annoying pests of humans and livestock in the Aras River Basin, Iran. However, there is no evidence of host preferenc...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-020-04432-4
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abstract：BACKGROUND:Release of gene-drive mutants to suppress Anopheles mosquito reproduction is a promising method of malaria control. However, many scientific, regulatory and ethical questions remain before transgenic mosquitoes can be utilised in the field. At a behavioural level, gene-drive carrying mutants should be at lea...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-020-04382-x
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journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-4-218
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abstract：BACKGROUND:The role of endemic murid rodents as hosts of arthropod vectors of diseases of medical and veterinary significance is well established in the northern hemisphere. In contrast, endemic murids are comparatively understudied as vector hosts in Africa, particularly in South Africa. Considering the great rodent d...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-7-366
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abstract：BACKGROUND:The South-to-North Water Diversion Project (SNWDP) is the largest national water conservancy project in China. However, the Eastern Route Project (ERP) of SNWDP will refer to the habitats of Oncomelania hupensis, the intermediate host of Schistosoma japonicum. The present study was aimed at investigating the...
journal_title：Parasites & vectors
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journal_title：Parasites & vectors
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doi：10.1186/s13071-020-04230-y
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journal_title：Parasites & vectors
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journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-7-442
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journal_title：Parasites & vectors
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journal_title：Parasites & vectors
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journal_title：Parasites & vectors
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journal_title：Parasites & vectors
pub_type： 杂志文章
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journal_title：Parasites & vectors
pub_type： 杂志文章
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journal_title：Parasites & vectors
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journal_title：Parasites & vectors
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更新日期：2016-08-24 00:00:00
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journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-020-04265-1
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journal_title：Parasites & vectors
pub_type： 杂志文章
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journal_title：Parasites & vectors
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journal_title：Parasites & vectors
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journal_title：Parasites & vectors
pub_type： 杂志文章
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journal_title：Parasites & vectors
pub_type： 杂志文章
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journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-018-2622-1
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journal_title：Parasites & vectors
pub_type： 杂志文章
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journal_title：Parasites & vectors
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journal_title：Parasites & vectors
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journal_title：Parasites & vectors
pub_type： 杂志文章
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journal_title：Parasites & vectors
pub_type： 杂志文章
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journal_title：Parasites & vectors
pub_type： 杂志文章
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journal_title：Parasites & vectors
pub_type： 杂志文章
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journal_title：Parasites & vectors
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更新日期：2018-07-04 00:00:00
