Abstract：
:Diabetes foot infections are a common condition and a major causal pathway to lower extremity amputation. Identification of causative pathogens is vital in directing antimicrobial therapy. Historically, clinicians have relied upon culture-dependent techniques that are now acknowledged as both being selective for microorganisms that thrive under the physiological and nutritional constraints of the microbiology laboratory and that grossly underestimate the microbial diversity of a sample. The amplification and sequence analysis of the 16S rRNA gene has revealed a diversity of microorganisms in diabetes foot infections, extending the view of the diabetic foot microbiome. The interpretation of these findings and their relevance to clinical care remains largely unexplored. The advent of molecular methods that are culture-independent and employ massively parallel DNA sequencing technology represents a potential 'game changer'. Metagenomics and its shotgun approach to surveying all DNA within a sample (whole genome sequencing) affords the possibility to characterize not only the microbial diversity within a diabetes foot infection (i.e. 'which microorganisms are present') but the biological functions of the community such as virulence and pathogenicity (i.e. 'what are the microorganisms capable of doing'), moving the focus from single species as pathogens to groups of species. This review will examine the new molecular techniques for exploration of the microbiome of infected and uninfected diabetic foot ulcers, exploring the potential of these new technologies and postulating how they could translate to improved clinical care. Copyright © 2016 John Wiley & Sons, Ltd.
journal_name
Diabetes Metab Res Revjournal_title
Diabetes/metabolism research and reviewsauthors
Malone M,Gosbell IB,Dickson HG,Vickery K,Espedido BA,Jensen SOdoi
10.1002/dmrr.2834subject
Has Abstractpub_date
2017-01-01 00:00:00issue
1eissn
1520-7552issn
1520-7560journal_volume
33pub_type
杂志文章,评审abstract：AIMS:Insulin-like growth factor 1 receptor (IGF1R) is involved in cell growth and glucose homeostasis. In the current study, the IGF1R locus was analysed as a candidate gene for type 2 diabetes (T2D) in American Indians. MATERIALS AND METHODS:Whole genome sequence data from 335 American Indians identified 3 novel miss...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章
doi：10.1002/dmrr.2994
更新日期：2018-05-01 00:00:00
abstract：:This perspective covers a novel area of research describing the inadequacies of current approaches for diagnosing dysglycaemia and proposes that the 1-hour post-load glucose level during the 75-g oral glucose tolerance test may serve as a novel biomarker to detect dysglycaemia earlier than currently recommended screen...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章
doi：10.1002/dmrr.2992
更新日期：2018-07-01 00:00:00
abstract：BACKGROUND:The incidence of type 1 diabetes mellitus (T1DM) has increased in recent decades, as has the incidence of preterm births (<37 weeks). We aimed to evaluate and compare the prevalence of prematurity and early prematurity (<34 weeks) and birth season variability among T1DM and non-T1DM children. METHODS:A nati...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章
doi：10.1002/dmrr.2996
更新日期：2018-07-01 00:00:00
abstract：:Excess visceral fat can regulate insulin sensitivity and energy metabolism by releasing adipokines into the circulation which then bind with their cognate receptors in various tissues and alter glucose and lipid metabolism. Circulating levels of adiponectin, which promotes glucose uptake into skeletal muscle and incre...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章,评审
doi：10.1002/dmrr.778
更新日期：2007-11-01 00:00:00
abstract：BACKGROUND:Diabetes mellitus is an endocrine disorder which is characterized by the development of resistance to the cellular activity of insulin or inadequate insulin production. It leads to hyperglycemia, prolonged inflammation, and oxidative stress. Oxidative stress is assumed to play an important role in the develo...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章
doi：10.1002/dmrr.3060
更新日期：2018-11-01 00:00:00
abstract：BACKGROUND:This study examined whether temporal trends exist in treatment of patients with type 2 diabetes (T2D) and quality of diabetes care after implementation of quality improvement initiative in primary care setting. METHODS:We conducted a population-based retrospective cohort study of 202,284 patients with T2D w...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章
doi：10.1002/dmrr.2952
更新日期：2018-02-01 00:00:00
abstract：BACKGROUND:In patients with type 1 diabetes, three main variables need to be assessed to optimize meal-related insulin boluses: pre-meal blood glucose (BG), insulin to carbohydrate ratio (I : C), and basal insulin. We are presenting data for a novel use of the hyperinsulinaemic-euglycaemic clamp (HEC) in patients with ...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章
doi：10.1002/dmrr.727
更新日期：2007-09-01 00:00:00
abstract：BACKGROUND:Leptin's hematopoietic or proinflammatory role has been experimentally reported. We investigated whether serum leptin concentrations are associated with white blood cell (WBC) counts in humans. METHODS:Serum leptin concentrations of Japanese civil servants aged 40 to 59 years (1082 men and 200 women) were a...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章
doi：10.1002/dmrr.540
更新日期：2005-09-01 00:00:00
abstract：:Various different systems have been proposed to classify diabetic foot ulcers, but none has gained widespread acceptance. The International Working Group of the Diabetic Foot (IWGDF) developed a classification system for research purposes, which is described in this report. In this PEDIS system, all foot ulcers should...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章,评审
doi：10.1002/dmrr.464
更新日期：2004-05-01 00:00:00
abstract：:The article's objective is to review the key advances in the scientific literature related to the association of stroke with diabetes mellitus and to summarize the current approaches to stroke prevention in diabetic patients. The key findings from the literature regarding stroke incidence in patients with diabetes, sp...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章,评审
doi：10.1002/dmrr.490
更新日期：2004-07-01 00:00:00
abstract：:In Mediterranean countries, the incidence (per 100,000 per year) of Type 1 diabetes in children aged under 15 years shows wide variation from country to country, ranging from 2.45 in Macedonia to 34.4 in Sardinia. By interacting with environmental factors such as diet, toxins or viral infections, the HLA plus non-HLA ...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章,评审
doi：10.1002/(sici)1520-7560(199903/04)15:2<133::aid-dm
更新日期：1999-03-01 00:00:00
abstract：BACKGROUND:To investigate longitudinal associations of maternal glucose/HbA1c and insulin dose with birthweight-related outcomes in women with type 1 diabetes. METHODS:We performed a cohort study including 473 pregnant women with type 1 diabetes with singleton pregnancies. We investigated maternal self-monitored blood...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章
doi：10.1002/dmrr.3300
更新日期：2020-07-01 00:00:00
abstract：BACKGROUND:Variants in TCF7L2 have been associated with the age at onset of type 2 diabetes in Mexican Americans. However, there is a lack of data on this relationship in Caucasians. Furthermore, risk alleles in TCF7L2 have been suggested to account for decreased conversion of proinsulin to insulin and decreased expres...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章
doi：10.1002/dmrr.1194
更新日期：2011-07-01 00:00:00
abstract：BACKGROUND:Body mass index (BMI) is associated with death in a V-shaped manner in general populations but it is unknown whether BMI or other risk factors also exhibit V-shaped relationships with death in type 2 diabetic patients. METHODS:A prospective cohort of 7534 Chinese, type 2 diabetic patients enrolled since 199...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章
doi：10.1002/dmrr.792
更新日期：2008-03-01 00:00:00
abstract：:Non-invasive tests for the detection of peripheral artery disease (PAD) among individuals with diabetes mellitus are important to estimate the risk of amputation, ulceration, wound healing and the presence of cardiovascular disease, yet there are no consensus recommendations to support a particular diagnostic modality...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章,评审
doi：10.1002/dmrr.2703
更新日期：2016-01-01 00:00:00
abstract：BACKGROUND:The correlation between serum uric acid (SUA) and β-cell function remains controversial. The present study aims to use a new index, renal function-normalized SUA, to observe its correlation with β-cell function in patients with type 2 diabetes (T2DM). METHODS:A total of 713 patients with T2DM received stand...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章
doi：10.1002/dmrr.3001
更新日期：2018-07-01 00:00:00
abstract：:Diabetic foot ulceration poses a heavy burden on the patient and the healthcare system, but prevention thereof receives little attention. For every euro spent on ulcer prevention, ten are spent on ulcer healing, and for every randomized controlled trial conducted on prevention, ten are conducted on healing. In this ar...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章
doi：10.1002/dmrr.2738
更新日期：2016-01-01 00:00:00
abstract：:There are a number of medical conditions such as growth failure in children, pregnancy, lipid abnormalities, and early complications that are improved by the meticulous glycemic control that can be achieved with insulin pump therapy (CSII). By using an insulin pump, many patients with severe hypoglycemia, the dawn phe...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章,评审
doi：10.1002/(sici)1520-7560(199909/10)15:5<338::aid-dm
更新日期：1999-09-01 00:00:00
abstract：BACKGROUND:We conducted a meta-analysis to investigate the association of circulating tumor necrosis factor-1 (TNFR-1) and TNFR-2 with diabetic kidney disease (DKD) progression, which is the first-ever quantitative analysis of these associations thus far. Whether TNFRs were better than albumin-creatinine ratio (ACR) in...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章,meta分析
doi：10.1002/dmrr.3195
更新日期：2019-11-01 00:00:00
abstract：BACKGROUND:The present study was undertaken to determine whether there were changes evident in axonal membrane function prior to the onset of neuropathy in patients with type 1 and type 2 diabetes. METHODS:From a cohort of 110 consecutive referrals, nerve excitability was investigated in 40 diabetic patients without c...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章
doi：10.1002/dmrr.2360
更新日期：2013-01-01 00:00:00
abstract：BACKGROUND:Current guidelines recommend screening the feet of diabetic subjects with a 10-g monofilament or tuning fork. We investigated which tests and locations on the feet have the best predictive value regarding 1-year ulcer-free survival in diabetic subjects participating in the prospective Rotterdam Diabetic Foot...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章
doi：10.1002/dmrr.3119
更新日期：2019-03-01 00:00:00
abstract：AIMS:This study was designed to investigate the association between pancreatic fat content (PFC) and insulin secretory capacity as well as glucose tolerance in Korean adults. MATERIALS:A total of 39 participants (mean age 49.9 years, 53% males) without a previous history of diabetes, or those previously diagnosed as h...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章
doi：10.1002/dmrr.3425
更新日期：2020-11-30 00:00:00
abstract：:Cardiovascular disease now is the leading cause of mortality among patients with type 1 diabetes (T1D). The risk of death from cardiovascular events in subjects with T1D is 2-10 times higher than the general population, depending on blood glucose control. Although complications of cardiovascular disease occur in middl...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章,评审
doi：10.1002/dmrr.3423
更新日期：2020-11-30 00:00:00
abstract：:In several large recent observational studies, peripheral arterial disease (PAD) was present in up to 50% of the patients with a diabetic foot ulcer and was an independent risk factor for amputation. The International Working Group on the Diabetic Foot therefore established a multidisciplinary working group to evaluat...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章,评审
doi：10.1002/dmrr.2249
更新日期：2012-02-01 00:00:00
abstract：:In addition to reducing hyperglycaemia, the metabolic actions of TZDs (pioglitazone and rosiglitazone) in theory might improve the prognosis of patients with type 2 diabetes. However, it appears from recent data that pioglitazone and rosiglitazone have different cardiovascular risk profiles. The scope of this paper is...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章,评审
doi：10.1002/dmrr.937
更新日期：2009-02-01 00:00:00
abstract：BACKGROUND:Increased activity of the hypothalamic-pituitary-adrenal (HPA) axis, resulting in enhanced adrenocorticotropin (ACTH) and serum glucocorticoid levels, has been described in patients with diabetes mellitus and in animal models of this disease; however, altered steroid production by adrenocortical cells could ...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章
doi：10.1002/dmrr.978
更新日期：2009-07-01 00:00:00
abstract：:Four kinds of subcutaneous continuous glucose monitoring (CGM) machines have been currently introduced in clinical practice. These machines exhibit real-time glucose on the monitor every 5 minutes and have alarms to indicate hypoglycaemia and hyperglycaemia. However, thus far, there is no clear consensus about the cli...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章,评审
doi：10.1002/dmrr.2346
更新日期：2012-12-01 00:00:00
abstract：BACKGROUND:The cardiovascular effects of sulfonylureas (SU) in diabetic patients are controversial and it has been suggested that diabetic patients with acute myocardial infarction while on SU were at increased risk. OBJECTIVES:To assess the in-hospital outcome of patients with acute myocardial infarction according to...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章,多中心研究
doi：10.1002/dmrr.498
更新日期：2005-03-01 00:00:00
abstract：BACKGROUND:Clinical studies have shown that proinsulin C-peptide exerts renoprotective effects in type 1 diabetes, although the underlying mechanisms are poorly understood. As C-peptide has been shown to induce several intracellular events and to localize to nuclei, we aimed to determine whether gene transcription is a...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章
doi：10.1002/dmrr.1220
更新日期：2011-10-01 00:00:00
abstract：:Small fibres constitute 70-90% of peripheral nerve fibres and regulate several key functions such as tissue blood flow, temperature and pain perception as well as sweating, all of which are highly relevant to the clinical presentation and adverse outcomes associated with foot ulcerations in patients with diabetes. Rec...
journal_title：Diabetes/metabolism research and reviews
pub_type： 杂志文章,实务指引
doi：10.1002/dmrr.1222
更新日期：2011-10-01 00:00:00
