当前位置： SCI文献检索 > Molecular Metabolism期刊下所有文献 > A comprehensive lipidomic screen of pancreatic β-cells using mass spectroscopy defines novel features of glucose-stimulated turnover of neutral lipids, sphingolipids and plasmalogens.

A comprehensive lipidomic screen of pancreatic β-cells using mass spectroscopy defines novel features of glucose-stimulated turnover of neutral lipids, sphingolipids and plasmalogens.

Abstract：

OBJECTIVE:Glucose promotes lipid remodelling in pancreatic β-cells, and this is thought to contribute to the regulation of insulin secretion, but the metabolic pathways and potential signalling intermediates have not been fully elaborated. METHODS:Using mass spectrometry (MS) we quantified changes in approximately 300 lipid metabolites in MIN6 β-cells and isolated mouse islets following 1 h stimulation with glucose. Flux through sphingolipid pathways was also assessed in (3)H-sphinganine-labelled cells using TLC. RESULTS:Glucose specifically activates the conversion of triacylglycerol (TAG) to diacylglycerol (DAG). This leads indirectly to the formation of 18:1 monoacylglycerol (MAG), via degradation of saturated/monounsaturated DAG species, such as 16:0_18:1 DAG, which are the most abundant, immediate products of glucose-stimulated TAG hydrolysis. However, 16:0-containing, di-saturated DAG species are a better direct marker of TAG hydrolysis since, unlike the 18:1-containing DAGs, they are predominately formed via this route. Using multiple reaction monitoring, we confirmed that in islets under basal conditions, 18:1 MAG is the most abundant species. We further demonstrated a novel site of glucose to enhance the conversion of ceramide to sphingomyelin (SM) and galactosylceramide (GalCer). Flux and product:precursor analyses suggest regulation of the enzyme SM synthase, which would constitute a separate mechanism for localized generation of DAG in response to glucose. Phosphatidylcholine (PC) plasmalogen (P) species, specifically those containing 20:4, 22:5 and 22:6 side chains, were also diminished in the presence of glucose, whereas the more abundant phosphatidylethanolamine plasmalogens were unchanged. CONCLUSION:Our results highlight 18:1 MAG, GalCer, PC(P) and DAG/SM as potential contributors to metabolic stimulus-secretion coupling.

journal_name

Mol Metab

journal_title

Molecular metabolism

authors

Pearson GL,Mellett N,Chu KY,Boslem E,Meikle PJ,Biden TJ

doi

10.1016/j.molmet.2016.04.003

subject

Has Abstract

pub_date

2016-04-13 00:00:00

pages

404-414

issue

6

issn

2212-8778

pii

S2212-8778(16)30020-5

journal_volume

5

pub_type

杂志文章

相关文献

Molecular Metabolism文献大全
  • Interdependence of nutrient metabolism and the circadian clock system: Importance for metabolic health.

    abstract：BACKGROUND:While additional research is needed, a number of large epidemiological studies show an association between circadian disruption and metabolic disorders. Specifically, obesity, insulin resistance, cardiovascular disease, and other signs of metabolic syndrome all have been linked to circadian disruption in hum...

    journal_title：Molecular metabolism

    pub_type： 杂志文章,评审

    doi：10.1016/j.molmet.2015.12.006

    authors： Ribas-Latre A,Eckel-Mahan K

    更新日期：2016-01-14 00:00:00

  • FGF21 resistance is not mediated by downregulation of beta-klotho expression in white adipose tissue.

    abstract：OBJECTIVE:Fibroblast growth factor 21 (FGF21) is an endocrine hormone that regulates metabolic homeostasis. Previous work has suggested that impairment of FGF21 signaling in adipose tissue may occur through downregulation of the obligate FGF21 co-receptor, β-klotho, which leads to "FGF21 resistance" during the onset of...

    journal_title：Molecular metabolism

    pub_type： 杂志文章

    doi：10.1016/j.molmet.2017.03.009

    authors： Markan KR,Naber MC,Small SM,Peltekian L,Kessler RL,Potthoff MJ

    更新日期：2017-03-27 00:00:00

  • Adipose specific disruption of seipin causes early-onset generalised lipodystrophy and altered fuel utilisation without severe metabolic disease.

    abstract：OBJECTIVE:Mutations to the BSCL2 gene disrupt the protein seipin and cause the most severe form of congenital generalised lipodystrophy (CGL). Affected individuals exhibit a near complete loss of white adipose tissue (WAT) and suffer from metabolic disease. Seipin is critical for adipocyte development in culture and mi...

    journal_title：Molecular metabolism

    pub_type： 杂志文章

    doi：10.1016/j.molmet.2018.01.019

    authors： Mcilroy GD,Suchacki K,Roelofs AJ,Yang W,Fu Y,Bai B,Wallace RJ,De Bari C,Cawthorn WP,Han W,Delibegović M,Rochford JJ

    更新日期：2018-04-01 00:00:00

  • Mitochondrial dysfunction has divergent, cell type-dependent effects on insulin action.

    abstract：:The contribution of mitochondrial dysfunction to insulin resistance is a contentious issue in metabolic research. Recent evidence implicates mitochondrial dysfunction as contributing to multiple forms of insulin resistance. However, some models of mitochondrial dysfunction fail to induce insulin resistance, suggesting...

    journal_title：Molecular metabolism

    pub_type： 杂志文章

    doi：10.1016/j.molmet.2014.02.001

    authors： Martin SD,Morrison S,Konstantopoulos N,McGee SL

    更新日期：2014-03-12 00:00:00

  • Action and therapeutic potential of oxyntomodulin.

    abstract：:Oxyntomodulin (OXM) is a peptide hormone released from the gut in post-prandial state that activates both the glucagon-like peptide-1 receptor (GLP1R) and the glucagon receptor (GCGR) resulting in superior body weight lowering to selective GLP1R agonists. OXM reduces food intake and increases energy expenditure in hum...

    journal_title：Molecular metabolism

    pub_type： 杂志文章,评审

    doi：10.1016/j.molmet.2013.12.001

    authors： Pocai A

    更新日期：2013-12-14 00:00:00

  • Activation of natriuretic peptides and the sympathetic nervous system following Roux-en-Y gastric bypass is associated with gonadal adipose tissues browning.

    abstract：OBJECTIVE:Roux-en-Y gastric bypass (RYGB) is an effective method of weight loss and remediation of type-2 diabetes; however, the mechanisms leading to these improvements are unclear. Additionally, adipocytes within white adipose tissue (WAT) depots can manifest characteristics of brown adipocytes. These 'BRITE/beige' a...

    journal_title：Molecular metabolism

    pub_type： 杂志文章

    doi：10.1016/j.molmet.2015.02.006

    authors： Neinast MD,Frank AP,Zechner JF,Li Q,Vishvanath L,Palmer BF,Aguirre V,Gupta RK,Clegg DJ

    更新日期：2015-03-03 00:00:00

  • Functional analysis reveals differential effects of glutamate and MCH neuropeptide in MCH neurons.

    abstract：OBJECTIVES:Melanin-concentrating hormone (MCH) neurons in the lateral hypothalamus (LH) regulate food intake and body weight, glucose metabolism and convey the reward value of sucrose. In this report, we set out to establish the respective roles of MCH and conventional neurotransmitters in these neurons. METHODS:MCH n...

    journal_title：Molecular metabolism

    pub_type： 杂志文章

    doi：10.1016/j.molmet.2018.05.001

    authors： Schneeberger M,Tan K,Nectow AR,Parolari L,Caglar C,Azevedo E,Li Z,Domingos A,Friedman JM

    更新日期：2018-07-01 00:00:00

  • Metabolic dysfunction in polycystic ovary syndrome: Pathogenic role of androgen excess and potential therapeutic strategies.

    abstract：BACKGROUND:Polycystic ovary syndrome (PCOS) is the most common endocrinopathy among reproductive age women. Although its cardinal manifestations include hyperandrogenism, oligo/anovulation, and/or polycystic ovarian morphology, PCOS women often display also notable metabolic comorbidities. An array of pathogenic mechan...

    journal_title：Molecular metabolism

    pub_type： 杂志文章,评审

    doi：10.1016/j.molmet.2020.01.001

    authors： Sanchez-Garrido MA,Tena-Sempere M

    更新日期：2020-05-01 00:00:00

  • Epidermal Acyl-CoA-binding protein is indispensable for systemic energy homeostasis.

    abstract：OBJECTIVES:The skin is the largest sensory organ of the human body and plays a fundamental role in regulating body temperature. However, adaptive alterations in skin functions and morphology have only vaguely been associated with physiological responses to cold stress or sensation of ambient temperatures. We previously...

    journal_title：Molecular metabolism

    pub_type： 杂志文章

    doi：10.1016/j.molmet.2020.101144

    authors： Neess D,Kruse V,Marcher AB,Wæde MR,Vistisen J,Møller PM,Petersen R,Brewer JR,Ma T,Colleluori G,Severi I,Cinti S,Gerhart-Hines Z,Mandrup S,Færgeman NJ

    更新日期：2020-12-18 00:00:00

  • Parental obesity leads to metabolic changes in the F2 generation in Drosophila.

    abstract：OBJECTIVE:A significant portion of the heritable risk for complex metabolic disorders cannot be attributed to classic Mendelian genetic factors. At least some of this missing heritability is thought to be due to the epigenetic influence of parental and grandparental metabolic state on offspring health. Previous work su...

    journal_title：Molecular metabolism

    pub_type： 杂志文章

    doi：10.1016/j.molmet.2017.03.012

    authors： Palu RAS,Praggastis SA,Thummel CS

    更新日期：2017-04-26 00:00:00

  • Profiling plasma N-Acylethanolamine levels and their ratios as a biomarker of obesity and dysmetabolism.

    abstract：OBJECTIVE:N-acylethanolamines play different roles in energy balance; anandamide (AEA) stimulates energy intake and storage, N-palmitoylethanolamide (PEA) counters inflammation, and N-oleoylethanolamide (OEA) mediates anorectic signals and lipid oxidation. Inconsistencies in the association of plasma N-acylethanolamine...

    journal_title：Molecular metabolism

    pub_type： 杂志文章

    doi：10.1016/j.molmet.2018.06.002

    authors： Fanelli F,Mezzullo M,Repaci A,Belluomo I,Ibarra Gasparini D,Di Dalmazi G,Mastroroberto M,Vicennati V,Gambineri A,Morselli-Labate AM,Pasquali R,Pagotto U

    更新日期：2018-08-01 00:00:00

  • 14-3-3ζ mediates an alternative, non-thermogenic mechanism in male mice to reduce heat loss and improve cold tolerance.

    abstract：OBJECTIVE:Adaptive thermogenesis, which is partly mediated by sympathetic input on brown adipose tissue (BAT), is a mechanism of heat production that confers protection against prolonged cold exposure. Various endogenous stimuli, for example, norepinephrine and FGF-21, can also promote the conversion of inguinal white ...

    journal_title：Molecular metabolism

    pub_type： 杂志文章

    doi：10.1016/j.molmet.2020.101052

    authors： Diallo K,Dussault S,Noll C,Lopez AF,Rivard A,Carpentier AC,Lim GE

    更新日期：2020-11-01 00:00:00

  • Metabolic functions of the tumor suppressor p53: Implications in normal physiology, metabolic disorders, and cancer.

    abstract：BACKGROUND:The TP53 gene is one of the most commonly inactivated tumor suppressors in human cancers. p53 functions during cancer progression have been linked to a variety of transcriptional and non-transcriptional activities that lead to the tight control of cell proliferation, senescence, DNA repair, and cell death. H...

    journal_title：Molecular metabolism

    pub_type： 杂志文章,评审

    doi：10.1016/j.molmet.2019.10.002

    authors： Lacroix M,Riscal R,Arena G,Linares LK,Le Cam L

    更新日期：2020-03-01 00:00:00

  • Preadipocytes of obese humans display gender-specific bioenergetic responses to glucose and insulin.

    abstract：BACKGROUND/OBJECTIVES:Although the prevalence of obesity and its associated metabolic disorders is increasing in both sexes, the clinical phenotype differs between men and women, highlighting the need for individual treatment options. Mitochondrial dysfunction in various tissues, including white adipose tissue (WAT), h...

    journal_title：Molecular metabolism

    pub_type： 杂志文章

    doi：10.1016/j.molmet.2018.11.006

    authors： Keuper M,Berti L,Raedle B,Sachs S,Böhm A,Fritsche L,Fritsche A,Häring HU,Hrabě de Angelis M,Jastroch M,Hofmann SM,Staiger H

    更新日期：2019-02-01 00:00:00

  • A Neural basis for Octanoic acid regulation of energy balance.

    abstract：OBJECTIVES:Nutrient sensing by hypothalamic neurons is critical for the regulation of food intake and energy expenditure. We aimed to identify long- and medium-chain fatty acid species transported into the brain, their effects on energy balance, and the mechanisms by which they regulate activity of hypothalamic neurons...

    journal_title：Molecular metabolism

    pub_type： 杂志文章

    doi：10.1016/j.molmet.2020.01.002

    authors： Haynes VR,Michael NJ,van den Top M,Zhao FY,Brown RD,De Souza D,Dodd GT,Spanswick D,Watt MJ

    更新日期：2020-04-01 00:00:00

  • Gut microbiota and glucometabolic alterations in response to recurrent partial sleep deprivation in normal-weight young individuals.

    abstract：OBJECTIVE:Changes to the microbial community in the human gut have been proposed to promote metabolic disturbances that also occur after short periods of sleep loss (including insulin resistance). However, whether sleep loss affects the gut microbiota remains unknown. METHODS:In a randomized within-subject crossover s...

    journal_title：Molecular metabolism

    pub_type： 杂志文章

    doi：10.1016/j.molmet.2016.10.003

    authors： Benedict C,Vogel H,Jonas W,Woting A,Blaut M,Schürmann A,Cedernaes J

    更新日期：2016-10-24 00:00:00

  • Cadm2 regulates body weight and energy homeostasis in mice.

    abstract：OBJECTIVE:Obesity is strongly linked to genes regulating neuronal signaling and function, implicating the central nervous system in the maintenance of body weight and energy metabolism. Genome-wide association studies identified significant associations between body mass index (BMI) and multiple loci near Cell adhesion...

    journal_title：Molecular metabolism

    pub_type： 杂志文章

    doi：10.1016/j.molmet.2017.11.010

    authors： Yan X,Wang Z,Schmidt V,Gauert A,Willnow TE,Heinig M,Poy MN

    更新日期：2018-02-01 00:00:00

  • The AMPK activator R419 improves exercise capacity and skeletal muscle insulin sensitivity in obese mice.

    abstract：OBJECTIVE:Skeletal muscle AMP-activated protein kinase (AMPK) is important for regulating glucose homeostasis, mitochondrial content and exercise capacity. R419 is a mitochondrial complex-I inhibitor that has recently been shown to acutely activate AMPK in myotubes. Our main objective was to examine whether R419 treatm...

    journal_title：Molecular metabolism

    pub_type： 杂志文章

    doi：10.1016/j.molmet.2015.06.002

    authors： Marcinko K,Bujak AL,Lally JS,Ford RJ,Wong TH,Smith BK,Kemp BE,Jenkins Y,Li W,Kinsella TM,Hitoshi Y,Steinberg GR

    更新日期：2015-06-15 00:00:00

  • Human beta cell mass and function in diabetes: Recent advances in knowledge and technologies to understand disease pathogenesis.

    abstract：BACKGROUND:Plasma insulin levels are predominantly the product of the morphological mass of insulin producing beta cells in the pancreatic islets of Langerhans and the functional status of each of these beta cells. Thus, deficiency in either beta cell mass or function, or both, can lead to insufficient levels of insuli...

    journal_title：Molecular metabolism

    pub_type： 杂志文章,评审

    doi：10.1016/j.molmet.2017.06.019

    authors： Chen C,Cohrs CM,Stertmann J,Bozsak R,Speier S

    更新日期：2017-07-08 00:00:00

  • Altered ghrelin secretion in mice in response to diet-induced obesity and Roux-en-Y gastric bypass.

    abstract：:The current study examined potential mechanisms for altered circulating ghrelin levels observed in diet-induced obesity (DIO) and following weight loss resulting from Roux-en-Y gastric bypass (RYGB). We hypothesized that circulating ghrelin levels were altered in obesity and after weight loss through changes in ghreli...

    journal_title：Molecular metabolism

    pub_type： 杂志文章

    doi：10.1016/j.molmet.2014.07.009

    authors： Uchida A,Zechner JF,Mani BK,Park WM,Aguirre V,Zigman JM

    更新日期：2014-08-01 00:00:00

  • Gut microbiota and immune crosstalk in metabolic disease.

    abstract：BACKGROUND:Gut microbiota is considered as a major regulator of metabolic disease. This reconciles the notion of metabolic inflammation and the epidemic development of the disease. In addition to evidence showing that a specific gut microbiota characterizes patients with obesity, type 2 diabetes, and hepatic steatosis,...

    journal_title：Molecular metabolism

    pub_type： 杂志文章,评审

    doi：10.1016/j.molmet.2016.05.016

    authors： Burcelin R

    更新日期：2016-06-06 00:00:00

  • Mapping the molecular signatures of diet-induced NASH and its regulation by the hepatokine Tsukushi.

    abstract：OBJECTIVE:Nonalcoholic steatohepatitis (NASH) is closely associated with metabolic syndrome and increases the risk for end-stage liver disease, such as cirrhosis and hepatocellular carcinoma. Despite this, the molecular events that influence NASH pathogenesis remain poorly understood. The objectives of the current stud...

    journal_title：Molecular metabolism

    pub_type： 杂志文章

    doi：10.1016/j.molmet.2018.12.004

    authors： Xiong X,Wang Q,Wang S,Zhang J,Liu T,Guo L,Yu Y,Lin JD

    更新日期：2019-02-01 00:00:00

  • 11β-Hydroxysteroid dehydrogenase-1 is involved in bile acid homeostasis by modulating fatty acid transport protein-5 in the liver of mice.

    abstract：:11β-Hydroxysteroid dehydrogenase-1 (11β-HSD1) plays a key role in glucocorticoid receptor (GR) activation. Besides, it metabolizes some oxysterols and bile acids (BAs). The GR regulates BA homeostasis; however, the impact of impaired 11β-HSD1 activity remained unknown. We profiled plasma and liver BAs in liver-specifi...

    journal_title：Molecular metabolism

    pub_type： 杂志文章

    doi：10.1016/j.molmet.2014.04.008

    authors： Penno CA,Morgan SA,Rose AJ,Herzig S,Lavery GG,Odermatt A

    更新日期：2014-05-02 00:00:00

  • Distinct adipocyte progenitor cells are associated with regional phenotypes of perivascular aortic fat in mice.

    abstract：OBJECTIVE:Perivascular adipose tissue depots around the aorta are regionally distinct and have specific functional properties. Thoracic aorta perivascular adipose tissue (tPVAT) expresses higher levels of thermogenic genes and lower levels of inflammatory genes than abdominal aorta perivascular adipose tissue (aPVAT). ...

    journal_title：Molecular metabolism

    pub_type： 杂志文章

    doi：10.1016/j.molmet.2017.12.014

    authors： Tran KV,Fitzgibbons T,Min SY,DeSouza T,Corvera S

    更新日期：2018-03-01 00:00:00

  • Neonatal GLP1R activation limits adult adiposity by durably altering hypothalamic architecture.

    abstract：OBJECTIVE:Adult obesity risk is influenced by alterations to fetal and neonatal environments. Modifying neonatal gut or neurohormone signaling pathways can have negative metabolic consequences in adulthood. Here we characterize the effect of neonatal activation of glucagon like peptide-1 (GLP-1) receptor (GLP1R) signal...

    journal_title：Molecular metabolism

    pub_type： 杂志文章

    doi：10.1016/j.molmet.2017.05.006

    authors： Rozo AV,Babu DA,Suen PA,Groff DN,Seeley RJ,Simmons RA,Seale P,Ahima RS,Stoffers DA

    更新日期：2017-05-15 00:00:00

  • Metabolic remodeling of dystrophic skeletal muscle reveals biological roles for dystrophin and utrophin in adaptation and plasticity.

    abstract：OBJECTIVES:Preferential damage to fast, glycolytic myofibers is common in many muscle-wasting diseases, including Duchenne muscular dystrophy (DMD). Promoting an oxidative phenotype could protect muscles from damage and ameliorate the dystrophic pathology with therapeutic relevance, but developing efficacious strategie...

    journal_title：Molecular metabolism

    pub_type： 杂志文章

    doi：10.1016/j.molmet.2020.101157

    authors： Hardee JP,Martins KJB,Miotto PM,Ryall JG,Gehrig SM,Reljic B,Naim T,Chung JD,Trieu J,Swiderski K,Philp AM,Philp A,Watt MJ,Stroud DA,Koopman R,Steinberg GR,Lynch GS

    更新日期：2020-12-24 00:00:00

  • The taming of PARP1 and its impact on NAD+ metabolism.

    abstract：BACKGROUND:Poly-ADP-ribose polymerases (PARPs) are key mediators of cellular stress response. They are intimately linked to cellular metabolism through the consumption of NAD+. PARP1/ARTD1 in the nucleus is the major NAD+ consuming activity and plays a key role in maintaining genomic integrity. SCOPE OF REVIEW:In this...

    journal_title：Molecular metabolism

    pub_type： 杂志文章,评审

    doi：10.1016/j.molmet.2020.01.014

    authors： Hurtado-Bagès S,Knobloch G,Ladurner AG,Buschbeck M

    更新日期：2020-08-01 00:00:00

  • Receptor structure-based discovery of non-metabolite agonists for the succinate receptor GPR91.

    abstract：OBJECTIVE:Besides functioning as an intracellular metabolite, succinate acts as a stress-induced extracellular signal through activation of GPR91 (SUCNR1) for which we lack suitable pharmacological tools. METHODS AND RESULTS:Here we first determined that the cis conformation of the succinate backbone is preferred and ...

    journal_title：Molecular metabolism

    pub_type： 杂志文章

    doi：10.1016/j.molmet.2017.09.005

    authors： Trauelsen M,Rexen Ulven E,Hjorth SA,Brvar M,Monaco C,Frimurer TM,Schwartz TW

    更新日期：2017-12-01 00:00:00

  • Adult neural stem cell fate is determined by thyroid hormone activation of mitochondrial metabolism.

    abstract：OBJECTIVE:In the adult brain, neural stem cells (NSCs) located in the subventricular zone (SVZ) produce both neuronal and glial cells. Thyroid hormones (THs) regulate adult NSC differentiation towards a neuronal phenotype, but also have major roles in mitochondrial metabolism. As NSC metabolism relies mainly on glycoly...

    journal_title：Molecular metabolism

    pub_type： 杂志文章

    doi：10.1016/j.molmet.2017.08.003

    authors： Gothié JD,Sébillot A,Luongo C,Legendre M,Nguyen Van C,Le Blay K,Perret-Jeanneret M,Remaud S,Demeneix BA

    更新日期：2017-11-01 00:00:00

  • High-fidelity Glucagon-CreER mouse line generated by CRISPR-Cas9 assisted gene targeting.

    abstract：OBJECTIVE:α-cells are the second most prominent cell type in pancreatic islets and are responsible for producing glucagon to increase plasma glucose levels in times of fasting. α-cell dysfunction and inappropriate glucagon secretion occur in both type 1 and type 2 diabetes. Thus, there is growing interest in studying b...

    journal_title：Molecular metabolism

    pub_type： 杂志文章

    doi：10.1016/j.molmet.2017.01.003

    authors： Ackermann AM,Zhang J,Heller A,Briker A,Kaestner KH

    更新日期：2017-01-12 00:00:00