Abstract:
OBJECTIVE:Changes to the microbial community in the human gut have been proposed to promote metabolic disturbances that also occur after short periods of sleep loss (including insulin resistance). However, whether sleep loss affects the gut microbiota remains unknown. METHODS:In a randomized within-subject crossover study utilizing a standardized in-lab protocol (with fixed meal times and exercise schedules), we studied nine normal-weight men at two occasions: after two nights of partial sleep deprivation (PSD; sleep opportunity 02:45-07:00 h), and after two nights of normal sleep (NS; sleep opportunity 22:30-07:00 h). Fecal samples were collected within 24 h before, and after two in-lab nights, of either NS or PSD. In addition, participants underwent an oral glucose tolerance test following each sleep intervention. RESULTS:Microbiota composition analysis (V4 16S rRNA gene sequencing) revealed that after two days of PSD vs. after two days of NS, individuals exhibited an increased Firmicutes:Bacteroidetes ratio, higher abundances of the families Coriobacteriaceae and Erysipelotrichaceae, and lower abundance of Tenericutes (all P < 0.05) - previously all associated with metabolic perturbations in animal or human models. However, no PSD vs. NS effect on beta diversity or on fecal short-chain fatty acid concentrations was found. Fasting and postprandial insulin sensitivity decreased after PSD vs. NS (all P < 0.05). DISCUSSION:Our findings demonstrate that short-term sleep loss induces subtle effects on human microbiota. To what extent the observed changes to the microbial community contribute to metabolic consequences of sleep loss warrants further investigations in larger and more prolonged sleep studies, to also assess how sleep loss impacts the microbiota in individuals who already are metabolically compromised.
journal_name
Mol Metabjournal_title
Molecular metabolismauthors
Benedict C,Vogel H,Jonas W,Woting A,Blaut M,Schürmann A,Cedernaes Jdoi
10.1016/j.molmet.2016.10.003subject
Has Abstractpub_date
2016-10-24 00:00:00pages
1175-1186issue
12issn
2212-8778pii
S2212-8778(16)30193-4journal_volume
5pub_type
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doi:10.1016/j.molmet.2017.09.005
更新日期:2017-12-01 00:00:00
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doi:10.1016/j.molmet.2017.05.006
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doi:10.1016/j.molmet.2016.04.003
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doi:10.1016/j.molmet.2016.02.001
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doi:10.1016/j.molmet.2018.10.003
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pub_type: 杂志文章,评审
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更新日期:2019-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.molmet.2014.12.005
更新日期:2014-12-19 00:00:00
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pub_type: 杂志文章
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journal_title:Molecular metabolism
pub_type: 杂志文章,评审
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更新日期:2016-12-21 00:00:00