Abstract:
BACKGROUND:Active cancer immunotherapies are beginning to yield clinical benefit, especially those using peptide-pulsed dendritic cells (DCs). Different adjuvants, including Toll-like receptor (TLR) agonists, commonly co-administered to cancer patients as part of a DC-based vaccine, are being widely tested in the clinical setting. However, endogenous DCs in tumor-bearing individuals are often dysfunctional, suggesting that ex vivo educated DCs might be superior inducers of anti-tumor immune responses. We have previously shown that prothymosin alpha (proTα) and its immunoreactive decapeptide proTα(100-109) induce the maturation of human DCs in vitro. The aim of this study was to investigate whether proTα- or proTα(100-109)-matured DCs are functionally competent and to provide preliminary evidence for the mode of action of these agents. RESULTS:Monocyte-derived DCs matured in vitro with proTα or proTα(100-109) express co-stimulatory molecules and secrete pro-inflammatory cytokines. ProTα- and proTα(100-109)-matured DCs pulsed with HER-2/neu peptides induce TH1-type immune responses, prime autologous naïve CD8-positive (+) T cells to lyse targets expressing the HER-2/neu epitopes and to express a polyfunctional profile, and stimulate CD4+ T cell proliferation in an HER-2/neu peptide-dependent manner. DC maturation induced by proTα and proTα(100-109) is likely mediated via TLR-4, as shown by assessing TLR-4 surface expression and the levels of the intracellular adaptor molecules TIRAP, MyD88 and TRIF. CONCLUSIONS:Our results suggest that proTα and proTα(100-109) induce both the maturation and the T cell stimulatory capacity of DCs. Although further studies are needed, evidence for a possible proTα and proTα(100-109) interaction with TLR-4 is provided. The initial hypothesis that proTα and the proTα-derived immunoactive decapeptide act as "alarmins", provides a rationale for their eventual use as adjuvants in DC-based anti-cancer immunotherapy.
journal_name
BMC Immunoljournal_title
BMC immunologyauthors
Ioannou K,Derhovanessian E,Tsakiri E,Samara P,Kalbacher H,Voelter W,Trougakos IP,Pawelec G,Tsitsilonis OEdoi
10.1186/1471-2172-14-43subject
Has Abstractpub_date
2013-09-22 00:00:00pages
43issn
1471-2172pii
1471-2172-14-43journal_volume
14pub_type
杂志文章相关文献
BMC IMMUNOLOGY文献大全abstract:BACKGROUND:Bv8, prokineticin-1, or endocrine gland-vascular endothelial growth factor, and prokineticin-2 are recently isolated peptide agonists of two G protein-coupled receptors, prokineticin receptor-1 (PROKR 1) and PROKR 2, and have been described as affecting a number of myeloid cell functions. We evaluated the im...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-9-60
更新日期:2008-10-28 00:00:00
abstract:BACKGROUND:We have developed a 12-parameter/10-color flow cytometric staining method for the simultaneous detection and characterization of 21 mouse thymocyte subpopulations that represent discreet stages of T cell development. To demonstrate the utility of this method, we assessed cytokine receptor expression on mouse...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-12-36
更新日期:2011-06-20 00:00:00
abstract:BACKGROUND:Functioning of the immune system requires the coordinated expression and action of many genes and proteins. With the emergence of high-throughput technologies, a great amount of molecular data is available for the genes and proteins of the immune system. However, these data are scattered into several databas...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-10-3
更新日期:2009-01-09 00:00:00
abstract:BACKGROUND:Non-adherence has been associated with reduced graft survival. The aim of this study was to investigate the immunological mechanisms underlying chronic renal allograft rejection using a model of non-adherence to immunosuppressive therapy. We used a MHC (major histocompatibility complex) -mismatched rat model...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-017-0236-6
更新日期:2017-12-19 00:00:00
abstract:BACKGROUND:Human Papillomavirus, HPV, is the main etiological factor for cervical cancer. Different studies show that in women infected with HPV there is a positive correlation between lesion grade and number of infiltrating macrophages, as well as with IL-10 higher expression. Using a HPV16 associated tumor model in m...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-11-27
更新日期:2010-06-07 00:00:00
abstract:BACKGROUND:The CXCR3 receptor and its three interferon-inducible ligands (CXCL9, CXCL10 and CXCL11) have been implicated as playing a central role in directing a Th1 inflammatory response. Recent studies strongly support that the CXCR3 receptor is a very attractive therapeutic target for treating autoimmune diseases, s...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-13-2
更新日期:2012-01-10 00:00:00
abstract:BACKGROUND:While tumor necrosis factor alpha (TNF-α) inhibitors (TNFi) and other biologics are very effective against autoimmune diseases, they can also cause infectious diseases. Therefore, it is important to clarify whether the TNFi sometimes used to treat patients with rheumatoid arthritis (RA) complicated with huma...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-017-0191-2
更新日期:2017-02-03 00:00:00
abstract:BACKGROUND:Macrophages are key players in the initiation, perpetuation and regulation of both innate and adaptive immune responses. They largely perform these roles through modulation of the expression of genes, especially those encoding cytokines. Murine bone marrow derived macrophages (BMDMs) are commonly used as a m...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-017-0223-y
更新日期:2017-10-03 00:00:00
abstract:BACKGROUND:The role of M2 polarized macrophages (MΦ) during the allergic airway inflammation has been discussed in various animal models. However, their presence and relevance during the chronic and acute phase of allergic airway inflammation in humans has not been fully elucidated so far. In the present study we pheno...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-15-12
更新日期:2014-03-10 00:00:00
abstract:BACKGROUND:While vaccination remains the most effective method to control hepatitis B virus (HBV) infection, 5-10% of recipients exhibit non-responsiveness to the HB vaccine. Immunological analysis of strong, weak or absent protective antibody responses to the HB vaccine should provide insights into the mechanisms that...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-018-0263-y
更新日期:2018-07-28 00:00:00
abstract:BACKGROUND:In genetically modified (GM) crops there is a risk that the inserted genes may introduce new allergens and/or adjuvants into the food and feed chain. The MON810 maize, expressing the insecticidal Cry1Ab toxin, is grown in many countries worldwide. In animal models, intranasal and intraperitoneal immunisation...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-016-0148-x
更新日期:2016-05-04 00:00:00
abstract:BACKGROUND:The initiation of highly active antiretroviral therapy (HAART) plays a significant role in the clinical management of HIV infected people by preventing morbidity and mortality. This benefit becomes, the most terrible when treatment failure develops. Thus, this research aims to assess the prevalence and assoc...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-018-0278-4
更新日期:2018-12-17 00:00:00
abstract:BACKGROUND:Marginal zone (MZ) B cells play important roles in the early phases of humoral immune responses. In addition to possessing an inherent capacity to rapidly differentiate into antibody secreting cells, MZ B cells also help to regulate the fate of both T-independent and T-dependent blood-borne antigens in the s...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-6-8
更新日期:2005-04-18 00:00:00
abstract:BACKGROUND:Proinflammatory genes are highly expressed in several metabolic disorders associated with obesity. But it is not clarified whether gene expression levels and downstream inflammatory markers are related to the metabolic state or the presence of obesity. Hence, the present study aimed to compare Toll-Like Rece...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-019-0303-2
更新日期:2019-07-04 00:00:00
abstract:BACKGROUND:There are three major B-cell compartments in peripheral lymphoid organs: the germinal center (GC), the mantle zone (MNZ) and the marginal zone (MGZ). Unique sets of B-cells reside in these compartments, and they have specific functional roles in humoral immune response. MNZ B cells are naive cells in a quies...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-5-20
更新日期:2004-09-15 00:00:00
abstract:BACKGROUND:Cryopreservation of peripheral blood mononuclear cells (PBMCs) is a common and essential practice in conducting research. There are different reports in the literature as to whether cryopreserved PBMCs need to only be stored ≤ -150 °C or can be stored for a specified time at -80 °C. Therefore, we performed g...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-016-0144-1
更新日期:2016-03-15 00:00:00
abstract:BACKGROUND:Streptococcus sanguinis (S.sanguinis), a predominant bacterium in the human oral cavity, has been widely associated with the development of infective endocarditis. Platelets play both a haemostatic function and can influence both innate and adaptive immune responses. Previous studies have shown that S.sangui...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-15-15
更新日期:2014-04-22 00:00:00
abstract:BACKGROUND:Dendritic cells (DC) play the key role in directing antigen-specific immune responses and manipulating their function may be a useful tool for immunotherapy. The balance between immune stimulation and tolerance is particularly important at mucosal interfaces, where discrimination between dangerous pathogens ...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-9-10
更新日期:2008-03-18 00:00:00
abstract:BACKGROUND:Deer mice (Peromyscus maniculatus) are the most common mammals in North America and are reservoirs for several zoonotic agents, including Sin Nombre virus (SNV), the principal etiologic agent of hantavirus cardiopulmonary syndrome (HCPS) in North America. Unlike human HCPS patients, SNV-infected deer mice sh...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-7-18
更新日期:2006-08-17 00:00:00
abstract:BACKGROUND:Shiga toxin 2 (Stx2), one of two Stx liberated by Stx-producing Escherichia coli, is composed of an A subunit monomer and a B subunit pentamer, and is directly linked with hemolytic uremic syndrome in children. The pentameric B subunit binds to its cell surface receptor Gb3 for toxin internalization, and the...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-11-16
更新日期:2010-03-24 00:00:00
abstract:BACKGROUND:Respiratory burst function resulting in the release of reactive oxygen species such as superoxide anion (O2-) from neutrophils is one of the key mechanisms of the innate immune system, and maladaptive control of this mechanism is thought to play a pivotal role in the development of pathologies such as acute ...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-10-25
更新日期:2009-05-08 00:00:00
abstract:BACKGROUND:CD4+CD25+ regulatory T cells play an essential role in maintaining immune homeostasis and preventing autoimmunity. Therefore, defects in Treg development, maintenance or function have been associated with several human autoimmune diseases including Systemic Lupus Erythematosus (SLE), a systemic autoimmune di...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-10-5
更新日期:2009-01-27 00:00:00
abstract:BACKGROUND:Most HIV-infected subjects exhibit a progressive rise in CD4 T-cell counts after initiation of highly active antiretroviral therapy (HAART). However, a subset of individuals exhibit very poor CD4 T-cell recovery despite effective control of HIV-RNA viraemia. We evaluated CD4 T-cell proliferation among subopt...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-14-26
更新日期:2013-06-20 00:00:00
abstract:BACKGROUND:Immunoglobulin rearrangement involves random and imprecise processes that act to both create and constrain diversity. Two such processes are the loss of nucleotides through the action of unknown exonuclease(s) and the addition of P nucleotides. The study of such processes has been compromised by difficulties...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-5-19
更新日期:2004-09-02 00:00:00
abstract:BACKGROUND:Myeloid cells, especially mononuclear phagocytes, which include monocytes, macrophages and dendritic cells (DC), play vital roles in innate immunity, and in the initiation and maintenance of adaptive immunity. While T cell-associated activation pathways and cytokines have been identified and evaluated in inf...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-019-0322-z
更新日期:2019-11-12 00:00:00
abstract:BACKGROUND:Understanding immune mechanisms, particularly the role of innate immune markers during latent TB infection remains elusive. The main objective of this study was to evaluate mRNA gene expression patterns of toll-like receptors (TLRs) as correlates of immunity during latent TB infection and further infer their...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-021-00400-4
更新日期:2021-01-28 00:00:00
abstract:BACKGROUND:Pro-inflammatory cytokines can affect intracellular lipid metabolism. A variety of effects have been described for different cell types; hepatocyte lipid turnover pathways are inhibited during inflammation, whereas interleukin-1beta (IL-1beta) reduces intracellular cholesterol levels in fibroblasts. Levels o...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-9-70
更新日期:2008-11-25 00:00:00
abstract:BACKGROUND:Molecular mimicry between microbial antigens and host-proteins is one of the etiological enigmas for the occurrence of autoimmune diseases. T cells that recognize cross-reactive epitopes may trigger autoimmune reactions. Intriguingly, autoimmune diseases have been reported to be prevalent in tuberculosis end...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-13-13
更新日期:2012-03-21 00:00:00
abstract:BACKGROUND:To understand the role of genetic factors on chromosome 1 in the regulation of spontaneous arthritis in mice deficient in IL-1 receptor antagonist protein (IL_1RA), we previously used speed congenic breeding to transfer the QTL region from DBA/1(-/-) mice that are resistant to spontaneous arthritis into BALB...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-014-0057-9
更新日期:2014-12-09 00:00:00
abstract:BACKGROUND:Myeloid derived suppressor cells (MDSCs) present a significant obstacle to cancer immunotherapy because they dampen anti-tumor cytotoxic T cell responses. Previous groups, including our own, have reported on the myelo-depletive effects of certain chemotherapy agents. We have shown previously that decitabine ...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-020-0337-5
更新日期:2020-02-27 00:00:00