Abstract:
BACKGROUND:While tumor necrosis factor alpha (TNF-α) inhibitors (TNFi) and other biologics are very effective against autoimmune diseases, they can also cause infectious diseases. Therefore, it is important to clarify whether the TNFi sometimes used to treat patients with rheumatoid arthritis (RA) complicated with human T-lymphotropic virus type-I (HTLV-I) infection have the unintended side effect of promoting HTLV-I proliferation. METHODS:We used the HTLV-I-infected cell line HCT-5, derived from spinal fluid cells of a patient with HTLV-I associated myelopathy, to evaluate the production of cytokines and chemokines, TNF-α receptor (TNFR), the expression of HTLV-I associated genes, the HTLV-I proviral load (PVL), the expression of HTLV-I structural protein, and apoptosis. We used Jurkat cells as a control. RESULTS:Supernatants of HCT-5 showed time-dependent elevations of IL-6, RANTES and ICAM-1. HCT-5 supernatants treated with infliximab, adalimumab, etanercept (ETN), golimumab and certolizumab pegol showed no significant differences in the levels of these molecules compared to the control. Neither TNFR1 nor TNFR2 expression was altered by any TNFi treatment, relative to phosphate-buffered saline (PBS) treatment, with the exception that TNFR2 was significantly decreased and internalized in HCT-5 cells by ETN treatment. The HTLV-I associated genes Tax and HBZ and the PVL levels were not significantly changed. Immunofluorescence staining of HCT-5 for an HTLV-I-associated protein, GAG, was also not significantly different between any of the TNFi treatments and the PBS treatment. DNA ladders as an index of apoptosis were not detected. Apoptotic cells were not increased by the addition of any TNFi. CONCLUSIONS:In vitro, TNFi did not affect the cytokine profiles, expression of associated genes and proteins, proviral load or apoptosis of HCT-5 cells. The results suggested that TNFi treatment of RA patients complicated with HTLV-I might have no effect on HTLV-I infection.
journal_name
BMC Immunoljournal_title
BMC immunologyauthors
Fukui S,Nakamura H,Takahashi Y,Iwamoto N,Hasegawa H,Yanagihara K,Nakamura T,Okayama A,Kawakami Adoi
10.1186/s12865-017-0191-2subject
Has Abstractpub_date
2017-02-03 00:00:00pages
7issue
1issn
1471-2172pii
10.1186/s12865-017-0191-2journal_volume
18pub_type
杂志文章相关文献
BMC IMMUNOLOGY文献大全abstract:BACKGROUND:Shiga toxin 2 (Stx2), one of two Stx liberated by Stx-producing Escherichia coli, is composed of an A subunit monomer and a B subunit pentamer, and is directly linked with hemolytic uremic syndrome in children. The pentameric B subunit binds to its cell surface receptor Gb3 for toxin internalization, and the...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-11-16
更新日期:2010-03-24 00:00:00
abstract:BACKGROUND:Neutrophils are critical in the defense against potentially harmful microorganisms, but their excessive and inappropriate activation can contribute significantly to tissue damage and a worsening pathology. Through the release of endocrine factors submandibular glands contribute to achieving a balance in neut...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-4-3
更新日期:2003-03-04 00:00:00
abstract:BACKGROUND:Non-adherence has been associated with reduced graft survival. The aim of this study was to investigate the immunological mechanisms underlying chronic renal allograft rejection using a model of non-adherence to immunosuppressive therapy. We used a MHC (major histocompatibility complex) -mismatched rat model...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-017-0236-6
更新日期:2017-12-19 00:00:00
abstract:BACKGROUND:While dendritic cells (DCs) can induce tolerance in T cells, little is known about tolerance induction in DCs themselves. We have analysed tolerance induced in human in-vitro generated DCs by repeated stimulation with ligands for TLR4 and TLR2. RESULTS:DCs stimulated with the TLR4 ligand LPS did show a rapi...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-9-69
更新日期:2008-11-24 00:00:00
abstract:BACKGROUND:The potential utility of dendritic cells (DC) as cancer vaccines has been established in early trials in human cancers. The concomitant administration of cytotoxic agents and DC vaccines has been previously avoided due to potential immune suppression by chemotherapeutics. Recent studies show that common chem...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-11-14
更新日期:2010-03-19 00:00:00
abstract:BACKGROUND:Some chemicals, including some phthalate plasticizers, have been shown to have an adjuvant effect in mice. However, an adjuvant effect, defined as an inherent ability to stimulate the humoral immune response, was only observed after exposure to a limited number of the phthalates. An adjuvant effect may be du...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-9-61
更新日期:2008-10-31 00:00:00
abstract:BACKGROUND:Egg white must provide nutrients and protection to the developing avian embryo. One way in which this is achieved is an arsenal of antimicrobial proteins and peptides which are essentially extensions of the innate immune system. Gallin is a recently identified member of a family of peptides that are found in...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-11-12
更新日期:2010-03-12 00:00:00
abstract:BACKGROUND:Tuberculosis (TB) remains a serious human health problem that affects millions of people in the world. Understanding the biology of Mycobacterium tuberculosis (Mtb) is essential for tackling this devastating disease. Mtb possesses a very complex cell envelope containing a variety of lipid components that par...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-015-0102-3
更新日期:2015-06-23 00:00:00
abstract:BACKGROUND:Parasite-specific IgE levels correlate with human resistance to reinfection with Schistosoma spp. after chemotherapy. Although the role of eosinophils in schistosomiasis has been the focus of a great deal of important research, the involvement of other Fcepsilon receptor-bearing cells, such as mast cells and...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-5-6
更新日期:2004-04-21 00:00:00
abstract:BACKGROUND:Gene transfer studies in primary T cells have suffered from the limitations of conventional viral transduction or transfection techniques. Replication-defective adenoviral vectors are an attractive alternative for gene delivery. However, naive lymphocytes are not readily susceptible to infection with adenovi...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-3-4
更新日期:2002-05-02 00:00:00
abstract:BACKGROUND:Myeloid cells, especially mononuclear phagocytes, which include monocytes, macrophages and dendritic cells (DC), play vital roles in innate immunity, and in the initiation and maintenance of adaptive immunity. While T cell-associated activation pathways and cytokines have been identified and evaluated in inf...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-019-0322-z
更新日期:2019-11-12 00:00:00
abstract:BACKGROUND:The role of M2 polarized macrophages (MΦ) during the allergic airway inflammation has been discussed in various animal models. However, their presence and relevance during the chronic and acute phase of allergic airway inflammation in humans has not been fully elucidated so far. In the present study we pheno...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-15-12
更新日期:2014-03-10 00:00:00
abstract:BACKGROUND:To understand the role of genetic factors on chromosome 1 in the regulation of spontaneous arthritis in mice deficient in IL-1 receptor antagonist protein (IL_1RA), we previously used speed congenic breeding to transfer the QTL region from DBA/1(-/-) mice that are resistant to spontaneous arthritis into BALB...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-014-0057-9
更新日期:2014-12-09 00:00:00
abstract:BACKGROUND:Adenoviral vectors have provided effective methods for in vivo gene delivery in therapeutic applications. However, these vectors can induce immune responses that may severely affect the ability of vector re-application. There is limited information about the mechanisms and signal transduction pathways involv...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-12-8
更新日期:2011-01-21 00:00:00
abstract:BACKGROUND:Symptomatic Primary Humoral Immunodeficiency Diseases (PHID) constitute a highly heterogeneous group of diseases characterized by a shared hypogammaglobulinemia, resulting in increased risk of recurrent or severe infections. Associations have been described with a variety of immunological abnormalities invol...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-15-13
更新日期:2014-03-12 00:00:00
abstract:BACKGROUND:Pattern recognition receptors (PRRs) for double-stranded RNA (dsRNA) are components of innate immunity that recognize the presence of viral infection and initiate efficient defense mechanisms. In addition to previously well-characterized signaling pathways that are mediated by PKR and TLR3, new intracellular...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-12-15
更新日期:2011-02-10 00:00:00
abstract:BACKGROUND:The USDA, Wildlife Services cooperative oral rabies vaccination (ORV) program uses a live vaccinia virus-vectored (genus Orthopoxvirus) vaccine, Raboral V-RG (V-RG), to vaccinate specific wildlife species against rabies virus in several regions of the U.S. Several naturally occurring orthopoxviruses have bee...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-9-57
更新日期:2008-10-03 00:00:00
abstract:BACKGROUND:Immunophenotyping of blood lymphocytes is an essential tool to evaluate the immune function of patients with immunodeficiency or autoimmunity. Predominately identified CD4+T cell subsets, Th1, Th2, Th17, as well as regulatory T (Treg) cells, play crucial roles in several immunological and pathological condit...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-020-00374-9
更新日期:2020-08-03 00:00:00
abstract:BACKGROUND:Recent studies have shown that early growth response 2 (EGR2) is highly induced in activated T cells and regulates T cell functions. In normal C57BL/6 (B6) mice, deletion of EGR2 in lymphocytes results in the development of lupus-like systemic autoimmune disease, which implies indirectly an autoimmune protec...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-020-00370-z
更新日期:2020-07-09 00:00:00
abstract:BACKGROUND:Functioning of the immune system requires the coordinated expression and action of many genes and proteins. With the emergence of high-throughput technologies, a great amount of molecular data is available for the genes and proteins of the immune system. However, these data are scattered into several databas...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-10-3
更新日期:2009-01-09 00:00:00
abstract:BACKGROUND:We sought to compare Pneumovax®23 responses in adults with subnormal IgG subclass concentrations. We studied adults with normal total IgG, frequent/severe respiratory infection, and subnormal IgG1, IgG3, or IgG1 + IgG3 before and after Pneumovax®23. We defined response as serotype-specific IgG > 1.3 μg/mL an...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-019-0310-3
更新日期:2019-08-20 00:00:00
abstract:BACKGROUND:The need for rapid and accurate comparison of panels of adjuvanted vaccine formulations and subsequent rational down selection, presents several challenges for modern vaccine development. Here we describe a method which may enable vaccine and adjuvant developers to compare antigen/adjuvant combinations in a ...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-018-0245-0
更新日期:2018-01-31 00:00:00
abstract:AIM:Brucellar spondylitis (BS) is one of the most serious complications of brucellosis. CXCR3 is closely related to the severity of disease infection. This research aimed to study the degree of BS inflammatory damage through analyzing the expression levels of CXCR3 and its ligands (CXCL9 and CXCL10) in patients with BS...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-020-00390-9
更新日期:2020-11-18 00:00:00
abstract:BACKGROUND:Besides their prominent role in the elimination of infected or malignantly transformed cells, natural killer (NK) cells serve as modulators of adaptive immune responses. Enhancing bidirectional crosstalk between NK cells and dendritic cells (DC) is considered a promising tool to potentiate cancer vaccines. W...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-018-0247-y
更新日期:2018-02-13 00:00:00
abstract:BACKGROUND:Dysregulation of the immune system has been shown to occur during spaceflight, although the detailed nature of the phenomenon and the clinical risks for exploration class missions have yet to be established. Also, the growing clinical significance of immune system evaluation combined with epidemic infectious...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-8-7
更新日期:2007-05-23 00:00:00
abstract:BACKGROUND:Respiratory burst function resulting in the release of reactive oxygen species such as superoxide anion (O2-) from neutrophils is one of the key mechanisms of the innate immune system, and maladaptive control of this mechanism is thought to play a pivotal role in the development of pathologies such as acute ...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-10-25
更新日期:2009-05-08 00:00:00
abstract:BACKGROUND:The purpose of this study was to characterize specific cytotoxic T-cell (CTL) responses in men who have sex with men (MSM) subjects infected with the human immunodeficiency virus type 1 (HIV-1) CRF01_AE subtype during the first year of infection and impacts on viral control and evolution. RESULTS:Fifteen HI...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-016-0166-8
更新日期:2016-08-30 00:00:00
abstract:BACKGROUND:The lymphatic vascular pattern in the head of mice has rarely been studied, due to problems of sectioning and immunostaining of complex bony structures. Therefore, the association of head lymphoid tissues with the lymphatics has remained unknown although the mouse is the most often used species in immunology...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-018-0242-3
更新日期:2018-01-25 00:00:00
abstract:BACKGROUND:Cryopreservation of peripheral blood mononuclear cells has been used to preserve and standardize immunologic measurements for multicenter studies, however, effects of cryopreservation on cytokine responses are incompletely understood. In designing immunologic studies for a new multicenter birth cohort study ...
journal_title:BMC immunology
pub_type: 杂志文章,多中心研究
doi:10.1186/1471-2172-7-29
更新日期:2006-12-12 00:00:00
abstract:BACKGROUND:Proinflammatory genes are highly expressed in several metabolic disorders associated with obesity. But it is not clarified whether gene expression levels and downstream inflammatory markers are related to the metabolic state or the presence of obesity. Hence, the present study aimed to compare Toll-Like Rece...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-019-0303-2
更新日期:2019-07-04 00:00:00