Efficient adenovirus-mediated gene transfer into primary T cells and thymocytes in a new coxsackie/adenovirus receptor transgenic model.


BACKGROUND:Gene transfer studies in primary T cells have suffered from the limitations of conventional viral transduction or transfection techniques. Replication-defective adenoviral vectors are an attractive alternative for gene delivery. However, naive lymphocytes are not readily susceptible to infection with adenoviruses due to insufficient expression of the coxsackie/adenovirus receptor. RESULTS:To render T cells susceptible to adenoviral gene transfer, we have developed three new murine transgenic lines in which expression of the human coxsackie/adenovirus receptor (hCAR) with a truncated cytoplasmic domain (hCAR(Delta)cyt) is limited to thymocytes and lymphocytes under direction of a human CD2 mini-gene. hCAR(Delta)cyt.CD2 transgenic mice were crossed with DO11.10 T cell receptor transgenic mice (DO11.hCAR(Delta)cyt) to allow developmental studies in a defined, clonal T cell population. Expression of hCAR(Delta)cyt enabled adenoviral transduction of resting primary CD4+ T cells, differentiated effector T cells and thymocytes from DO11.hCAR(Delta)cyt with high efficiency. Expression of hCAR(Delta)cyt transgene did not perturb T cell development in these mice and adenoviral transduction of DO11.hCAR(Delta)cyt T cells did not alter their activation status, functional responses or differentiative potential. Adoptive transfer of the transduced T cells into normal recipients did not modify their physiologic localization. CONCLUSION:The DO11.hCAR(Delta)cyt transgenic model thus allows efficient gene transfer in primary T cell populations and will be valuable for novel studies of T cell activation and differentiation.


BMC Immunol


BMC immunology


Hurez V,Dzialo-Hatton R,Oliver J,Matthews RJ,Weaver CT





Has Abstract


2002-05-02 00:00:00








  • Streptococcus pneumoniae stabilizes tumor necrosis factor alpha mRNA through a pathway dependent on p38 MAPK but independent of Toll-like receptors.

    abstract:BACKGROUND:Streptococcus pneumoniae is a human pathogenic bacteria and a major cause of severe invasive diseases, including pneumonia, bacteremia, and meningitis. Infections with S. pneumoniae evoke a strong inflammatory response, which plays a major role in the pathogenesis of pneumococcal disease. RESULTS:In this st...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Mogensen TH,Berg RS,Ostergaard L,Paludan SR

    更新日期:2008-09-16 00:00:00

  • Detection of IL-9 producing T cells in the PBMCs of allergic asthmatic patients.

    abstract:BACKGROUND:Interleukin-9 (IL-9) was reported as an active participant in the pathogenesis of allergic asthma. This study aimed to investigate the major source ofIL-9 and its effect on interferon γ (IFN-γ) and immunoglobulin (Ig) secretion by B cells. METHODS:We isolated peripheral blood mononuclear cells from children...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Jia L,Wang Y,Li J,Li S,Zhang Y,Shen J,Tan W,Wu C

    更新日期:2017-07-19 00:00:00

  • HLA class I supertypes: a revised and updated classification.

    abstract:BACKGROUND:Class I major histocompatibility complex (MHC) molecules bind, and present to T cells, short peptides derived from intracellular processing of proteins. The peptide repertoire of a specific molecule is to a large extent determined by the molecular structure accommodating so-called main anchor positions of th...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Sidney J,Peters B,Frahm N,Brander C,Sette A

    更新日期:2008-01-22 00:00:00

  • Differential utilization of NF-kappaB RELA and RELB in response to extracellular versus intracellular polyIC stimulation in HT1080 cells.

    abstract:BACKGROUND:Pattern recognition receptors (PRRs) for double-stranded RNA (dsRNA) are components of innate immunity that recognize the presence of viral infection and initiate efficient defense mechanisms. In addition to previously well-characterized signaling pathways that are mediated by PKR and TLR3, new intracellular...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Yun JJ,Tsao MS,Der SD

    更新日期:2011-02-10 00:00:00

  • Prothymosin α and a prothymosin α-derived peptide enhance T(H)1-type immune responses against defined HER-2/neu epitopes.

    abstract:BACKGROUND:Active cancer immunotherapies are beginning to yield clinical benefit, especially those using peptide-pulsed dendritic cells (DCs). Different adjuvants, including Toll-like receptor (TLR) agonists, commonly co-administered to cancer patients as part of a DC-based vaccine, are being widely tested in the clini...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Ioannou K,Derhovanessian E,Tsakiri E,Samara P,Kalbacher H,Voelter W,Trougakos IP,Pawelec G,Tsitsilonis OE

    更新日期:2013-09-22 00:00:00

  • Eosinophils may play regionally disparate roles in influencing IgA(+) plasma cell numbers during large and small intestinal inflammation.

    abstract:BACKGROUND:Eosinophils are innate immune cells present in the intestine during steady state conditions. An intestinal eosinophilia is a hallmark of many infections and an accumulation of eosinophils is also observed in the intestine during inflammatory disorders. Classically the function of eosinophils has been associa...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Forman R,Bramhall M,Logunova L,Svensson-Frej M,Cruickshank SM,Else KJ

    更新日期:2016-05-31 00:00:00

  • Selection of reliable reference genes for the normalisation of gene expression levels following time course LPS stimulation of murine bone marrow derived macrophages.

    abstract:BACKGROUND:Macrophages are key players in the initiation, perpetuation and regulation of both innate and adaptive immune responses. They largely perform these roles through modulation of the expression of genes, especially those encoding cytokines. Murine bone marrow derived macrophages (BMDMs) are commonly used as a m...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Tanaka A,To J,O'Brien B,Donnelly S,Lund M

    更新日期:2017-10-03 00:00:00

  • Characteristics and reference ranges of CD4+T cell subpopulations among healthy adult Han Chinese in Shanxi Province, North China.

    abstract:BACKGROUND:Immunophenotyping of blood lymphocytes is an essential tool to evaluate the immune function of patients with immunodeficiency or autoimmunity. Predominately identified CD4+T cell subsets, Th1, Th2, Th17, as well as regulatory T (Treg) cells, play crucial roles in several immunological and pathological condit...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Niu HQ,Zhao XC,Li W,Xie JF,Liu XQ,Luo J,Zhao WP,Li XF

    更新日期:2020-08-03 00:00:00

  • Depigmented-polymerised allergoids favour regulatory over effector T cells: enhancement by 1α, 25-dihydroxyvitamin D3.

    abstract:BACKGROUND:Allergen immunotherapy (SIT) is the only treatment for allergic disease capable of modifying disease long term. To reduce the risk of anaphylaxis from SIT, allergen-extracts have been modified by polymerisation with glutaraldehyde to reduce IgE binding. It is suggested that these allergoid extracts also have...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Urry ZL,Richards DF,Black C,Morales M,Carnés J,Hawrylowicz CM,Robinson DS

    更新日期:2014-05-29 00:00:00

  • Potential effect of prior raccoonpox virus infection in raccoons on vaccinia-based rabies immunization.

    abstract:BACKGROUND:The USDA, Wildlife Services cooperative oral rabies vaccination (ORV) program uses a live vaccinia virus-vectored (genus Orthopoxvirus) vaccine, Raboral V-RG (V-RG), to vaccinate specific wildlife species against rabies virus in several regions of the U.S. Several naturally occurring orthopoxviruses have bee...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Root JJ,McLean RG,Slate D,MacCarthy KA,Osorio JE

    更新日期:2008-10-03 00:00:00

  • Tolerance induced via TLR2 and TLR4 in human dendritic cells: role of IRAK-1.

    abstract:BACKGROUND:While dendritic cells (DCs) can induce tolerance in T cells, little is known about tolerance induction in DCs themselves. We have analysed tolerance induced in human in-vitro generated DCs by repeated stimulation with ligands for TLR4 and TLR2. RESULTS:DCs stimulated with the TLR4 ligand LPS did show a rapi...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Albrecht V,Hofer TP,Foxwell B,Frankenberger M,Ziegler-Heitbrock L

    更新日期:2008-11-24 00:00:00

  • The role of CXCR3 and its ligands expression in Brucellar spondylitis.

    abstract:AIM:Brucellar spondylitis (BS) is one of the most serious complications of brucellosis. CXCR3 is closely related to the severity of disease infection. This research aimed to study the degree of BS inflammatory damage through analyzing the expression levels of CXCR3 and its ligands (CXCL9 and CXCL10) in patients with BS...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Hu X,Shang X,Wang L,Fan J,Wang Y,Lv J,Nazierhan S,Wang H,Wang J,Ma X

    更新日期:2020-11-18 00:00:00

  • Co-localization of lymphoid aggregates and lymphatic networks in nose- (NALT) and lacrimal duct-associated lymphoid tissue (LDALT) of mice.

    abstract:BACKGROUND:The lymphatic vascular pattern in the head of mice has rarely been studied, due to problems of sectioning and immunostaining of complex bony structures. Therefore, the association of head lymphoid tissues with the lymphatics has remained unknown although the mouse is the most often used species in immunology...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Lohrberg M,Pabst R,Wilting J

    更新日期:2018-01-25 00:00:00

  • Modulation of pathogen-induced CCL20 secretion from HT-29 human intestinal epithelial cells by commensal bacteria.

    abstract:BACKGROUND:Human intestinal epithelial cells (IECs) secrete the chemokine CCL20 in response to infection by various enteropathogenic bacteria or exposure to bacterial flagellin. CCL20 recruits immature dendritic cells and lymphocytes to target sites. Here we investigated IEC responses to various pathogenic and commensa...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Sibartie S,O'Hara AM,Ryan J,Fanning A,O'Mahony J,O'Neill S,Sheil B,O'Mahony L,Shanahan F

    更新日期:2009-10-08 00:00:00

  • Gallin; an antimicrobial peptide member of a new avian defensin family, the ovodefensins, has been subject to recent gene duplication.

    abstract:BACKGROUND:Egg white must provide nutrients and protection to the developing avian embryo. One way in which this is achieved is an arsenal of antimicrobial proteins and peptides which are essentially extensions of the innate immune system. Gallin is a recently identified member of a family of peptides that are found in...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Gong D,Wilson PW,Bain MM,McDade K,Kalina J,Hervé-Grépinet V,Nys Y,Dunn IC

    更新日期:2010-03-12 00:00:00

  • EGR2 is elevated and positively regulates inflammatory IFNγ production in lupus CD4+ T cells.

    abstract:BACKGROUND:Recent studies have shown that early growth response 2 (EGR2) is highly induced in activated T cells and regulates T cell functions. In normal C57BL/6 (B6) mice, deletion of EGR2 in lymphocytes results in the development of lupus-like systemic autoimmune disease, which implies indirectly an autoimmune protec...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Dai R,Heid B,Xu X,Xie H,Reilly CM,Ahmed SA

    更新日期:2020-07-09 00:00:00

  • Increased expression of ICAM-1, VCAM-1, MCP-1, and MIP-1 alpha by spinal perivascular macrophages during experimental allergic encephalomyelitis in rats.

    abstract:BACKGROUND:T-cells extravasation and CNS parenchyma infiltration during autoimmune neurodegenerative disease can be evoked by local antigen presenting cells. Studying the chemoattracting potential of spinal perivascular macrophages (SPM) during experimental allergic encephalomyelitis (EAE), we observed numerous infiltr...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Hofmann N,Lachnit N,Streppel M,Witter B,Neiss WF,Guntinas-Lichius O,Angelov DN

    更新日期:2002-08-26 00:00:00

  • Distinct gene expression profiles in different B-cell compartments in human peripheral lymphoid organs.

    abstract:BACKGROUND:There are three major B-cell compartments in peripheral lymphoid organs: the germinal center (GC), the mantle zone (MNZ) and the marginal zone (MGZ). Unique sets of B-cells reside in these compartments, and they have specific functional roles in humoral immune response. MNZ B cells are naive cells in a quies...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Shen Y,Iqbal J,Xiao L,Lynch RC,Rosenwald A,Staudt LM,Sherman S,Dybkaer K,Zhou G,Eudy JD,Delabie J,McKeithan TW,Chan WC

    更新日期:2004-09-15 00:00:00

  • Functional requirements for inhibitory signal transmission by the immunomodulatory receptor CD300a.

    abstract:BACKGROUND:Activation signals can be negatively regulated by cell surface receptors bearing immunoreceptor tyrosine-based inhibitory motifs (ITIMs). CD300a, an ITIM bearing type I transmembrane protein, is expressed on many hematopoietic cells, including subsets of lymphocytes. RESULTS:We have taken two approaches to ...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: DeBell KE,Simhadri VR,Mariano JL,Borrego F

    更新日期:2012-04-26 00:00:00

  • High glucose concentrations induce TNF-α production through the down-regulation of CD33 in primary human monocytes.

    abstract:BACKGROUND:CD33 is a membrane receptor containing a lectin domain and a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) that is able to inhibit cytokine production. CD33 is expressed by monocytes, and reduced expression of CD33 correlates with augmented production of inflammatory cytokines, such as IL...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Gonzalez Y,Herrera MT,Soldevila G,Garcia-Garcia L,Fabián G,Pérez-Armendariz EM,Bobadilla K,Guzmán-Beltrán S,Sada E,Torres M

    更新日期:2012-04-14 00:00:00

  • CD4(+)CD25(High) Treg cells in HIV/HTLV co-infected patients with neuropathy: high expression of Alpha4 integrin and lower expression of Foxp3 transcription factor.

    abstract:BACKGROUND:Regulatory CD4 T cells (Tregs) are critical in maintaining the homeostasis of the immune system. Quantitative or phenotypic alterations and functional impairment of Tregs have been associated with the development of pathologies including those of the central nervous system. Individuals with HIV-1/HTLV-1 co-i...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Matavele Chissumba R,Silva-Barbosa SD,Augusto Â,Maueia C,Mabunda N,Gudo ES Jr,Bhatt N,Jani I,Savino W

    更新日期:2015-09-02 00:00:00

  • Pneumococcal vaccination responses in adults with subnormal IgG subclass concentrations.

    abstract:BACKGROUND:We sought to compare Pneumovax®23 responses in adults with subnormal IgG subclass concentrations. We studied adults with normal total IgG, frequent/severe respiratory infection, and subnormal IgG1, IgG3, or IgG1 + IgG3 before and after Pneumovax®23. We defined response as serotype-specific IgG > 1.3 μg/mL an...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Parker AR,Skold M,Harding S,Barton JC,Bertoli LF,Barton JC

    更新日期:2019-08-20 00:00:00

  • Multifaceted effects of synthetic TLR2 ligand and Legionella pneumophilia on Treg-mediated suppression of T cell activation.

    abstract:BACKGROUND:Regulatory T cells (Treg) play a crucial role in maintaining immune homeostasis and self-tolerance. The immune suppressive effects of Tregs should however be limited in case effective immunity is required against pathogens or cancer cells. We previously found that the Toll-like receptor 2 (TLR2) agonist, Pam...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: van Maren WW,Nierkens S,Toonen LW,Bolscher JM,Sutmuller RP,Adema GJ

    更新日期:2011-03-24 00:00:00

  • Changes in IgE- and antigen-dependent histamine-release in peripheral blood of Schistosoma mansoni-infected Ugandan fishermen after treatment with praziquantel.

    abstract:BACKGROUND:Parasite-specific IgE levels correlate with human resistance to reinfection with Schistosoma spp. after chemotherapy. Although the role of eosinophils in schistosomiasis has been the focus of a great deal of important research, the involvement of other Fcepsilon receptor-bearing cells, such as mast cells and...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Satti MZ,Cahen P,Skov PS,Joseph S,Jones FM,Fitzsimmons C,Hoffmann KF,Reimert C,Kariuki HC,Kazibwe F,Mwatha JK,Kimani G,Vennervald BJ,Ouma JH,Kabatereine NB,Dunne DW

    更新日期:2004-04-21 00:00:00

  • Evaluation of signal transduction pathways after transient cutaneous adenoviral gene delivery.

    abstract:BACKGROUND:Adenoviral vectors have provided effective methods for in vivo gene delivery in therapeutic applications. However, these vectors can induce immune responses that may severely affect the ability of vector re-application. There is limited information about the mechanisms and signal transduction pathways involv...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Steinstraesser L,Sorkin M,Jacobsen F,Al-Benna S,Kesting MR,Niederbichler AD,Otte JM,Hirsch T,Stupka J,Steinau HU,Schulte M

    更新日期:2011-01-21 00:00:00

  • Immune responses in the lungs of patients with tuberculous pleural effusion without pulmonary tuberculosis.

    abstract:BACKGROUND:Tuberculous pleural effusion (TPE) is one of the most common forms of extrapulmonary tuberculosis. Because most studies of TPE focused on the pleural space, little information regarding lung parenchyma is available. We therefore aimed to investigate immune responses in the lung parenchyma of TPE patients wit...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Qama D,Choi WI,Kwon KY

    更新日期:2012-08-13 00:00:00

  • NK cell predicts the severity of acute graft-versus-host disease in patients after allogeneic stem cell transplantation using antithymocyte globulin (ATG) in pretreatment scheme.

    abstract:BACKGROUND:Graft-versus-host disease (GVHD) is one of the most complex complications after allogeneic stem cell transplantation. Current standard of grading system is based on clinical symptoms in skin, liver and intestinal. However, it's difficult to differ GVHD and its extent just by clinical manifestation. Here we r...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Zhang P,Yang S,Zou Y,Yan X,Wu H,Zhou M,Sun YC,Zhang Y,Zhu H,Xu K,Wang Y,Sheng LX,Mu Q,Sun L,Ouyang G

    更新日期:2019-12-09 00:00:00

  • miR32-5p promoted vascular smooth muscle cell calcification by upregulating TNFα in the microenvironment.

    abstract:BACKGROUND:Vascular calcification is often associated with chronic inflammation and is a risk factor for brain arterial stiffness. Our previous results showed that miR32-5p was positively correlated with vascular smooth muscle cells (VSMC) calcification, but it is unclear whether miR32-5p promoted VSMC calcification by...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Cao J,Chen L,Zhong X,Shen Y,Gao Y,Chen Q,Zu X,Liu J

    更新日期:2020-01-17 00:00:00

  • The discriminative capacity of soluble Toll-like receptor (sTLR)2 and sTLR4 in inflammatory diseases.

    abstract:BACKGROUND:The extracellular domains of cytokine receptors are released during inflammation, but little is known about the shedding of Toll-like receptors (TLR) and whether they can be used as diagnostic biomarkers. METHODS:The release of sTLR2 and sTLR4 was studied in in-vitro stimulations, as well as in-vivo during ...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Ten Oever J,Kox M,van de Veerdonk FL,Mothapo KM,Slavcovici A,Jansen TL,Tweehuysen L,Giamarellos-Bourboulis EJ,Schneeberger PM,Wever PC,Stoffels M,Simon A,van der Meer JW,Johnson MD,Kullberg BJ,Pickkers P,Pachot A,Jooste

    更新日期:2014-11-19 00:00:00

  • Potential T cell epitopes of Mycobacterium tuberculosis that can instigate molecular mimicry against host: implications in autoimmune pathogenesis.

    abstract:BACKGROUND:Molecular mimicry between microbial antigens and host-proteins is one of the etiological enigmas for the occurrence of autoimmune diseases. T cells that recognize cross-reactive epitopes may trigger autoimmune reactions. Intriguingly, autoimmune diseases have been reported to be prevalent in tuberculosis end...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Chodisetti SB,Rai PK,Gowthaman U,Pahari S,Agrewala JN

    更新日期:2012-03-21 00:00:00