Abstract:
BACKGROUND:In this study, a total of 426 human faecal samples were examined for the presence of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii infection via a combination of microscopic examination and nested polymerase chain reaction (PCR) targeting 16S ribosomal RNA of Entamoeba species. METHODS:Faecal sample were collected from 426 participants in five rural villages in Peninsular Malaysia. The faecal samples were processed by direct wet smear and formalin ethyl acetate concentration technique followed by iodine staining and examined via microscopy for the presence of Entamoeba species and other intestinal parasites. Microscopically positive samples for Entamoeba species cysts were further characterized using a Nested Polymerase Chain Reaction (Nested-PCR) targeting 16S-like ribosomal RNA gene. The data entry and analysis was carried out using the SPSS software (Statistical Package for the Social Sciences) program for Windows version 17 (SPSS, Chicago, IL, USA). RESULTS:Based on single faecal examination, overall prevalence of Entamoeba infection was 17.6% (75/426). Females (19.1%) were more commonly infected compared to males (15.9%). Comparison by age groups showed that adults (23.9%) had higher infection rates than children (15.3%). The PCR results showed that 52 out of 75 microscopy positive samples successfully generated species-specific amplicons. The infection with E. histolytica (75.0%; 39/52) was the most common, followed by E. dispar (30.8%; 18/52) and E. moshkovskii (5.8%; 3/52). Of these, 33 (63.5%) were shown to contain only E. histolytica, 10 (19.2%) contained E. dispar and 3 (5.8%) contained only E. moshkovskii. Mixed infection with E. histolytica and E. dispar was found in 6 (11.5%) samples. CONCLUSIONS:The present study essentially emphasized the benefit of molecular techniques in discriminating the pathogenic Entamoeba species from the non-pathogenic for accurate diagnosis and better management of amoebiasis. The presence of E. moshkovskii is of great public health concern as it was the first time it has been reported in Malaysia.
journal_name
Parasit Vectorsjournal_title
Parasites & vectorsauthors
Ngui R,Angal L,Fakhrurrazi SA,Lian YL,Ling LY,Ibrahim J,Mahmud Rdoi
10.1186/1756-3305-5-187subject
Has Abstractpub_date
2012-09-04 00:00:00pages
187issn
1756-3305pii
1756-3305-5-187journal_volume
5pub_type
杂志文章abstract:BACKGROUND:Sheep scab is caused by the ectoparasitic mite Psoroptes ovis which initiates a profound cutaneous inflammatory response, leading to the development of the skin lesions which are characteristic of the disease. Existing control strategies rely upon injectable endectocides and acaricidal dips but concerns over...
journal_title:Parasites & vectors
pub_type: 杂志文章
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更新日期:2012-02-08 00:00:00
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journal_title:Parasites & vectors
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更新日期:2015-06-14 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
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更新日期:2018-03-07 00:00:00
abstract:BACKGROUND:Rhipicephalus sanguineus sensu lato (s.l.) is the most widely distributed ixodid tick and is a vector of major canine and human pathogens. High-throughput technologies have revealed that individual ticks carry a high diversity of pathogens, including bacteria, protozoa and viruses. Currently, it is accepted ...
journal_title:Parasites & vectors
pub_type: 杂志文章
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abstract:BACKGROUND:Following the recent discovery of the role of Anopheles rufipes Gough, 1910 in human malaria transmission in the northern savannah of Cameroon, we report here additional information on its feeding and resting habits and its susceptibility to the pyrethroid insecticide deltamethrin. METHODS:From 2011 to 2015...
journal_title:Parasites & vectors
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更新日期:2018-04-18 00:00:00
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journal_title:Parasites & vectors
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更新日期:2021-01-25 00:00:00
abstract:BACKGROUND:Dengue virus (DENV) is the arbovirus with the highest incidence in New Caledonia and in the South Pacific region. In 2012-2014, a major DENV-1 outbreak occurred in New Caledonia. The only known vector of DENV in New Caledonia is Aedes aegypti but no study has yet evaluated the competence of New Caledonia Ae....
journal_title:Parasites & vectors
pub_type: 杂志文章
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更新日期:2017-08-09 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
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journal_title:Parasites & vectors
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journal_title:Parasites & vectors
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更新日期:2013-05-01 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
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更新日期:2017-02-07 00:00:00
abstract:BACKGROUND:The tropical bed bug, Cimex hemipterus, is a serious indoor public health pest in tropical regions causing intense physical discomfort and mental distress to humans. At present, the application of insecticides is the major control strategy. The present study was designed to evaluate the development of resist...
journal_title:Parasites & vectors
pub_type: 杂志文章
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更新日期:2019-06-21 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-5-76
更新日期:2012-04-20 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-1148-z
更新日期:2015-10-12 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-1055-3
更新日期:2015-09-04 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3668-4
更新日期:2019-09-23 00:00:00
abstract:BACKGROUND:Tick cell lines are now available from fifteen ixodid and argasid species of medical and veterinary importance. However, some tick cell lines can be difficult to cryopreserve, and improved protocols for short- and long-term low temperature storage will greatly enhance their use as tools in tick and tick-born...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-3-37
更新日期:2010-04-13 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
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更新日期:2016-12-01 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-04420-8
更新日期:2020-10-27 00:00:00
abstract:BACKGROUND:The cysteine proteinases in papaya latex have been shown to have potent anthelmintic properties in monogastric hosts such as rodents, pigs and humans, but this has not been demonstrated in ruminants. METHODS:In two experiments, sheep were infected concurrently with 5,000 infective larvae of Haemonchus conto...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-4-36
更新日期:2011-03-15 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1508-3
更新日期:2016-04-23 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-0913-3
更新日期:2015-05-28 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-151
更新日期:2014-04-01 00:00:00
abstract:BACKGROUND:A good understanding of mosquito ecology is imperative for integrated vector control of malaria. In breeding sites, Anopheles larvae are concurrently exposed to predators and parasites. However, to our knowledge, there is no study on combined effects of predators and parasites on development and survival of ...
journal_title:Parasites & vectors
pub_type: 杂志文章,评审
doi:10.1186/s13071-020-3915-8
更新日期:2020-01-30 00:00:00
abstract:BACKGROUND:Toxoplasma gondii is an intracellular protozoan parasite that infects a wide variety of warm-blooded hosts, including humans. Limited information about T. gondii infection in bats is available in China. The objective of the present study was to determine prevalence and genetic characterization of T. gondii i...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-014-0558-7
更新日期:2014-12-03 00:00:00
abstract:BACKGROUND:Environmental changes caused by urbanization can cause alterations in the ecology and behavior of sandflies and in the epidemiology of leishmaniasis. Geotechnological tools allow the analysis and recognition of spatiotemporal patterns by monitoring and mapping risk areas of this vector-borne disease. This st...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-364
更新日期:2014-08-15 00:00:00
abstract:BACKGROUND:Despite intensive control measures including governmental campaigns using highly-efficacious systemic insecticides, there is evidence for persisting or recurring bovine Hypoderma species populations in parts of Europe, the USA and Canada. The present study evaluated the efficacy of LONGRANGE® (eprinomectin 5...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3475-y
更新日期:2019-05-14 00:00:00
abstract:BACKGROUND:The use of phytochemicals is a promising solution in biological control against salmon louse (Lepeophtheirus salmonis). Glucosinolates belong to a diverse group of compounds used as protection against herbivores by plants in the family Brassicaceae, while in vertebrates, ingested glucosinolates exert health-...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1537-y
更新日期:2016-05-10 00:00:00
abstract::Schistosomiasis is a major disease of public health importance in humans occurring in 76 countries of the tropics and sub-tropics. In China, schistosomiasis japonica is one of the highest priorities in communicable disease control defined by the central government. Since 1970s, the habitats of Biomphalaria straminea, ...
journal_title:Parasites & vectors
pub_type: 杂志文章,评审
doi:10.1186/1756-3305-6-249
更新日期:2013-08-28 00:00:00