High-throughput sorting of mosquito larvae for laboratory studies and for future vector control interventions.

Abstract:

BACKGROUND:Mosquito transgenesis offers new promises for the genetic control of vector-borne infectious diseases such as malaria and dengue fever. Genetic control strategies require the release of large number of male mosquitoes into field populations, whether they are based on the use of sterile males (sterile insect technique, SIT) or on introducing genetic traits conferring refractoriness to disease transmission (population replacement). However, the current absence of high-throughput techniques for sorting different mosquito populations impairs the application of these control measures. METHODS:A method was developed to generate large mosquito populations of the desired sex and genotype. This method combines flow cytometry and the use of Anopheles gambiae transgenic lines that differentially express fluorescent markers in males and females. RESULTS:Fluorescence-assisted sorting allowed single-step isolation of homozygous transgenic mosquitoes from a mixed population. This method was also used to select wild-type males only with high efficiency and accuracy, a highly desirable tool for genetic control strategies where the release of transgenic individuals may be problematic. Importantly, sorted males showed normal mating ability compared to their unsorted brothers. CONCLUSIONS:The developed method will greatly facilitate both laboratory studies of mosquito vectorial capacity requiring high-throughput approaches and future field interventions in the fight against infectious disease vectors.

journal_name

Malar J

journal_title

Malaria journal

authors

Marois E,Scali C,Soichot J,Kappler C,Levashina EA,Catteruccia F

doi

10.1186/1475-2875-11-302

subject

Has Abstract

pub_date

2012-08-28 00:00:00

pages

302

issn

1475-2875

pii

1475-2875-11-302

journal_volume

11

pub_type

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