Genetic deletion of dectin-1 does not affect the course of murine experimental colitis.

Abstract:

BACKGROUND:It is believed that inflammatory bowel diseases (IBD) result from an imbalance in the intestinal immune response towards the luminal microbiome. Dectin-1 is a widely expressed pattern recognition receptor that recognizes fungi and upon recognition it mediates cytokine responses and skewing of the adaptive immune system. Hence, dectin-1 may be involved in the pathogenesis of IBD. METHODS:We assessed the responses of dectin-1 deficient macrophages to the intestinal microbiota and determined the course of acute DSS and chronic Helicobacter hepaticus induced colitis in dectin-1 deficient mice. RESULTS:We show that the mouse intestinal microbiota contains fungi and the cytokine responses towards this microbiota were significantly reduced in dectin-1 deficient macrophages. However, in two different colitis models no significant differences in the course of inflammation were found in dectin-1 deficient mice compared to wild type mice. CONCLUSIONS:Together our data suggest that, although at the immune cell level there is a difference in response towards the intestinal flora in dectin-1 deficient macrophages, during intestinal inflammation this response seems to be redundant since dectin-1 deficiency in mice does not affect intestinal inflammation in experimental colitis.

journal_name

BMC Gastroenterol

journal_title

BMC gastroenterology

authors

Heinsbroek SE,Oei A,Roelofs JJ,Dhawan S,te Velde A,Gordon S,de Jonge WJ

doi

10.1186/1471-230X-12-33

subject

Has Abstract

pub_date

2012-04-16 00:00:00

pages

33

issn

1471-230X

pii

1471-230X-12-33

journal_volume

12

pub_type

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