Abstract:
BACKGROUND:The catalytic alpha and smaller beta subunits of the plasma membrane Na,K-ATPase occur in various molecular forms (alpha1, alpha2, alpha3, beta1 and beta2). The alpha isoforms of the enzyme have varying affinities for ouabain and exist in different tissues with particular distribution patterns. OBJECTIVE:To document the existence of isoforms of the Na,K-ATPase in cultured human umbilical vein endothelial cells. METHODS:Microsomal fractions were prepared by differential ultracentrifugation from primary cultures of human umbilical vein endothelial cells and from such cells obtained after three passages. Na,K-ATPase activity was assayed using the coupled assay method and sensitivity to ouabain was determined in the presence of varying concentrations of ouabain. Specific antibodies for the various Na,K-ATPase isoforms were used to label these different proteins by immunocytochemistry in endothelial cells and by Western blotting in isolated membranes. RESULTS:In plotting the dose-response curves for Na,K-ATPase activity in response to ouabain we assumed the existence of two independent sites exhibiting different affinities for ouabain (in the micromol/l and the nmol/l ranges). The contribution of low-affinity sites was threefold that of high-affinity sites. After three passages in culture, a specific increase in Na,K-ATPase activity of the high-affinity sites was observed compared with that of cells from primary cultures. Confocal microscopy revealed the existence of beta1, beta2, and alpha1 subunit proteins in human umbilical endothelial cells. Staining for alpha3 isoform was less pronounced and no obvious alpha2 was detected. CONCLUSION:These findings suggest that human umbilical vein endothelial cells contain beta1, beta2, a large amount of alpha1 isoform with an apparently low affinity for ouabain, and a lesser amount of high-affinity sites, which may correspond to the alpha3 protein.
journal_name
J Hypertensjournal_title
Journal of hypertensionauthors
Mayol V,Dignat-George F,Gerbi A,Martin-Vasallo P,Lesaule G,Sampol J,Maixent JMdoi
10.1097/00004872-199816020-00003subject
Has Abstractpub_date
1998-02-01 00:00:00pages
145-50issue
2eissn
0263-6352issn
1473-5598journal_volume
16pub_type
杂志文章abstract:OBJECTIVES:We hypothesized that the aldosterone: renin ratio (ARR) predicts the antihypertensive response to mineralocorticoid receptor antagonist, spironolactone (SPIRO), when compared with bendroflumethiazide (BFZ). METHODS:We conducted a randomized, crossover, trial on hypertensive patients with either high ARR (HA...
journal_title:Journal of hypertension
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/HJH.0b013e328332b79b
更新日期:2010-01-01 00:00:00
abstract:OBJECTIVE:To examine the effect on quality of life in hypertensive men of a multiple risk factor intervention programme compared with that of usual care. DESIGN:A prospective, open, randomized, parallel-group study with allocation either to a comprehensive multiple risk factor modification programme or to usual care. ...
journal_title:Journal of hypertension
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1995-12-01 00:00:00
abstract:OBJECTIVE:Endothelial dysfunction and arterial stiffness are early vascular alterations, linked to oxidative stress and inflammation, with prognostic significance in essential hypertensive patients. P2X7 receptors (P2X7R), responding to extracellular ATP, are encoded by a highly polymorphic gene and modulate inflammato...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0b013e3283653ff5
更新日期:2013-12-01 00:00:00
abstract::: The term 'cardiorenal syndrome' (CRS) was introduced to describe problems related to the simultaneous existence of heart and renal insufficiency. The prevalence of anaemia in CRS is high and increases the risk of hospitalizations and death. Renin-angiotensin system (RAS) inhibition is the cornerstone therapy in card...
journal_title:Journal of hypertension
pub_type: 杂志文章,评审
doi:10.1097/HJH.0000000000002111
更新日期:2019-11-01 00:00:00
abstract:OBJECTIVE:Inherited differences in renal function underlie the effect of high salt diets on blood pressure in Dahl rats. We probed the kidneys of inbred Dahl SS/Jr and SR/Jr for anonymous and candidate genes whose expression was regulated by dietary sodium. METHODS:mRNA quantitation of both candidate genes implicated ...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/01.hjh.0000133719.94075.e2
更新日期:2004-08-01 00:00:00
abstract:OBJECTIVE:We have recently shown that mean pulse pressure is higher in patients with transient myocardial ischemia. Pulse pressure elevation might be an important consequence of increased arterial stiffness. The aim of this study was to prove if arterial stiffness is changed in patients with transient myocardial ischem...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/01.hjh.0000244959.92856.7e
更新日期:2006-10-01 00:00:00
abstract:OBJECTIVE:To examine the influence of genetic factors on plasma leptin levels. SUBJECTS AND METHODS:We measured plasma leptin levels, body mass index and body fat distribution in healthy young female monozygotic (n = 19) and dizygotic (n = 14) twins. The twin zygosity was verified by determination of short tandem repe...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/00004872-199917010-00005
更新日期:1999-01-01 00:00:00
abstract:OBJECTIVE:Functional sex differences are described in several vascular beds. In the case of renal vessels, sex differences could influence processes like regulation of blood pressure and ion balance. Angiotensin II and nitric oxide are important regulators of renal vascular tone. Females have higher nitric oxide syntha...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0b013e32835731dd
更新日期:2012-09-01 00:00:00
abstract:OBJECTIVES:Globally, healthcare policy promotes supported self-management as a strategy for people with long-term conditions. This meta-review aimed to explore how people with hypertension make sense of their condition, to assess the effectiveness of supported self-management in hypertension, and to identify effective ...
journal_title:Journal of hypertension
pub_type: 杂志文章,评审
doi:10.1097/HJH.0000000000001867
更新日期:2019-02-01 00:00:00
abstract:OBJECTIVE:Various studies have suggested that a delay in the time between diagnosing hypertension and its correct control (D-C, diagnostic-control time) is linked to a worse prognosis. The aim of this study was to examine the relationship between D-C time and all-cause mortality, or the incidence of cardiovascular even...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0000000000001896
更新日期:2019-02-01 00:00:00
abstract:OBJECTIVE:Traditional cardiovascular risk factors do not fully account for ethnic differences in cardiovascular disease. We tested if arterial function indices, particularly augmentation index (AIx), and their determinants from childhood could underlie such ethnic variability among young British adults in the 'DASH' lo...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0000000000001066
更新日期:2016-11-01 00:00:00
abstract::The activity of renal Na-K-ATPase was compared in hypertension-prone (SBH) and hypertension-resistant (SBN) Sabra rats on regular sodium intake and 2-3 weeks after a high sodium diet. ATPase activity was determined in single nephron segments by a micromethod. The activity profile was found to be similar in the two sub...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:
更新日期:1983-06-01 00:00:00
abstract:OBJECTIVE:Similar to cancer, pulmonary arterial hypertension (PAH) is characterized by vascular remodeling, which leads to obliteration of the small pulmonary arteriole, with marked proliferation of pulmonary artery smooth muscle cells (PASMC) and/or endothelial cells dysfunction. Aberrant expression of tumor suppresso...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0000000000001475
更新日期:2017-12-01 00:00:00
abstract:BACKGROUND:The objective of this study was to estimate the prevalence and distribution of hypertension and to determine the status of hypertension awareness, treatment and control in the Portuguese adult population. METHODS:This study was conducted in 2003, and a multistage cluster sampling method was used to select a...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/01.hjh.0000179908.51187.de
更新日期:2005-09-01 00:00:00
abstract:BACKGROUND:Arterial stiffness is an independent predictor of cardiovascular events and mortality in hypertensive patients. The influence of different antihypertensive drug classes on improving arterial stiffness beyond blood pressure reduction is not widely available. We aimed to determine whether the artery stiffness ...
journal_title:Journal of hypertension
pub_type: 杂志文章,meta分析
doi:10.1097/HJH.0b013e328346a583
更新日期:2011-06-01 00:00:00
abstract:BACKGROUND:Recent evidence suggests that central aortic blood pressure may be a better predictor of cardiovascular risk than peripheral blood pressure. The central SBP (cSBP) can be estimated from the late systolic shoulder of the radial pulse waveform. We compared the second systolic peak of the radial waveform (pSBP(...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/hjh.0b013e32832e0b58
更新日期:2009-09-01 00:00:00
abstract:OBJECTIVES:In experimental animal models, exogenous aldosterone excess has been linked to the progression of renal disease. However, the evidence of an increased risk of renal damage in patients affected by primary aldosteronism remains controversial. We aimed at evaluating the association between primary aldosteronism...
journal_title:Journal of hypertension
pub_type: 杂志文章,meta分析
doi:10.1097/HJH.0000000000002216
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:Hypertension-induced renal injury is characterized by inflammation, fibrosis and proteinuria. Previous studies have demonstrated that N-acetyl-Ser-Asp-Lys-Pro (Ac-SDKP) inhibits renal damage following diabetes mellitus and antiglomerular basement membrane nephritis. However, its effects on low-renin hyperten...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0b013e32834103ee
更新日期:2011-02-01 00:00:00
abstract:OBJECTIVE:To search for a genetic marker of the sarco(endo)plasmic reticulum Ca(2+)-dependent ATPase (SERCA) II gene in spontaneously hypertensive rats (SHRs) and to investigate differences in blood pressure and intracellular Ca2+ among some substrains of SHRs and Wistar-Kyoto (WKY) rats related to their SERCA II genot...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/00004872-199603000-00003
更新日期:1996-03-01 00:00:00
abstract:BACKGROUND:Few studies have directly compared the effects of different angiotensin receptor blockers (ARBs) on mechanisms involved in the pathogenesis of cardiovascular disease. METHODS:We studied the ability of different ARBs to inhibit the proliferation of vascular smooth muscle cells (VSMCs) and cardiac fibroblasts...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0b013e3282f56ba5
更新日期:2008-05-01 00:00:00
abstract::: Altered blood pressure (BP) is a common phenomenon in acute ischemic stroke (AIS), with high BP being the most frequent scenario. The pathophysiology of BP changes in AIS is complex and only partially understood. The available evidence indicates that extremely high BP during AIS is associated with a poor outcome. Im...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0000000000001704
更新日期:2018-06-01 00:00:00
abstract:OBJECTIVE:We hypothesized that downregulation of the antioxidant thioredoxin system contributes to oxidative stress in angiotensin II-induced hypertension. As oestrogen may protect against oxidative stress, we also evaluated whether the thioredoxin system, particularly in the heart, is differentially regulated between ...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0b013e3280acac60
更新日期:2007-06-01 00:00:00
abstract::Arterial aging is associated with a progressive increase in aortic stiffening, which results in a rise in central aortic pressures. Traditionally, blood pressure (BP)-lowering therapy has been directed at brachial rather than central pressures. However, a key contemporary question is whether this strategy is optimal i...
journal_title:Journal of hypertension
pub_type: 杂志文章,评审
doi:10.1097/HJH.0b013e328353e523
更新日期:2012-06-01 00:00:00
abstract:OBJECTIVE:Epidemiological studies suggest that intrauterine growth restriction (IUGR) due to maternal undernutrition during pregnancy represents a major risk factor for hypertension and diabetes in adult age. However, placental insufficiency, rather than maternal malnutrition, is the main cause of IUGR in the Western w...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/00004872-199917090-00002
更新日期:1999-09-01 00:00:00
abstract:BACKGROUND:Familial hyperkalaemic hypertension (FHH) is a Mendelian form of low-renin hypertension characterized by hyperkalaemia and hyperchloraemic acidosis despite a normal glomerular filtration rate. To date, three different loci have been identified, on chromosomes 1, 17 and 12. OBJECTIVE:To test for genetic link...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/00004872-200111000-00005
更新日期:2001-11-01 00:00:00
abstract::Hypertensive disease is known to increase the risks in connection with acute changes in blood pressure due to the presence of pronounced structural as well as functional changes in the cardiovascular system. In the present study the metabolic consequences of fixed haemorrhagic hypotension [mean arterial pressure (MAP)...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/00004872-198310000-00005
更新日期:1983-10-01 00:00:00
abstract:OBJECTIVE:In essential hypertension, the mechanical properties of the radial artery have been shown to be largely unaltered, whereas more controversial and less reliable data have been obtained for the common carotid artery. We therefore examined the distensibility/pressure relationships of the predominantly elastic co...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/00004872-199715120-00070
更新日期:1997-12-01 00:00:00
abstract::Neutrophils possess a plasma-membrane-bound reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase which catalyzes superoxide (O2-) formation and is activated by a variety of stimuli. Recently, neutrophils of patients with essential hypertension (EHT) have been reported to generate O2- at rates up to four...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/00004872-199102000-00008
更新日期:1991-02-01 00:00:00
abstract::Adequate evaluation of automated sphygmomanometers, in terms of safety, accuracy, mechanical reliability, patient acceptability and ability to record ambulatory blood pressure is essential before these devices are used in clinical practice and in clinical trials. We have evaluated the accuracy and performance of the A...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/00004872-199007000-00003
更新日期:1990-07-01 00:00:00
abstract:BACKGROUND AND PURPOSE:The present study clarifies associations between stroke outcomes after intravenous tissue plasminogen activator (tPA) and blood pressure (BP) as well as heart rate (HR) profiles. METHODS:We assessed 125 patients with stroke who received tPA within 3 h of onset. We obtained baseline, mean, maximu...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0b013e32834a764e
更新日期:2011-10-01 00:00:00