Abstract：
:The effects of the trace metals zinc (Zn), manganese (Mn), and cadmium (Cd) on the metabolism of growth plate chondrocytes was examined using a mineralizing culture system. Supplementation of serum-free primary cultures of growth plate chondrocytes with 10-100 mu m Zn resulted in an increase in cell protein and greatly increased alkaline phosphatase (AP) activity; however, above 25 mu m Zn mineralization of the cultures was reduced. The effects of Zn on cellular protein and AP activity were enhanced by the addition of the albumin to the culture media. Removal of Zn from basal culture media resulted in recoverable reductions in cellular protein and AP activities. Cadmium was acutely toxic to chondrocyte cell cultures at concentrations above 5 mu m. Even at very low concentrations (0.25 mu m) Cd caused significant reductions in DNA, cellular protein, and matrix protein synthesis. In contrast, Cd had negligible effects on AP activity or culture mineralization. Manganese treatment (50 mu m) resulted in reduced levels of proteoglycan, cell protein, DNA synthesis, and collagen synthesis, although AP specific activity did not change. At 10 mu m, Mn significantly reduced mineralization but had only minor influence on other culture parameters. Both Zn (200 mu m) and Cd (0.1 mu m), but not Mn, induced the synthesis of metallothionein. The physiological and biochemical effects of specific metal ions is largely dependent on their physicochemical properties, especially their ligand affinities. Knowledge of these properties allows predictions to be made regarding whether the organic or the mineral phase are most likely to be affected in a mineralized tissue.
journal_name
Calcif Tissue Intjournal_title
Calcified tissue internationalauthors
Litchfield TM,Ishikawa Y,Wu LN,Wuthier RE,Sauer GRdoi
10.1007/s002239900442subject
Has Abstractpub_date
1998-04-01 00:00:00pages
341-9issue
4eissn
0171-967Xissn
1432-0827journal_volume
62pub_type
杂志文章abstract：:Fracture healing is a natural process that recapitulates embryonic skeletal development. In the early phase after fracture, reactive oxygen species (ROS) are produced under inflammatory and ischemic conditions due to vessel injury and soft tissue damage, leading to cell death. Usually, such damage during the course of...
journal_title：Calcified tissue international
pub_type： 杂志文章,评审
doi：10.1007/s00223-019-00576-3
更新日期：2019-10-01 00:00:00
abstract：:Bone loss occurs as early as the third decade and its cumulative effect throughout adulthood may impact risk for osteoporosis in later life, however, the genes and environmental factors influencing early bone loss are largely unknown. We investigated the role of genes in the change in bone mineral density (BMD) in par...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-008-9197-3
更新日期：2009-02-01 00:00:00
abstract：:We assessed the relationship of bone density and microarchitecture between hand, peripheral, and axial skeletal sites using high-resolution peripheral quantitative computed tomography (HR-pQCT) and dual-energy X-ray absorptiometry (DXA) in patients with rheumatoid arthritis (RA) and which factors influence these param...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-012-9644-z
更新日期：2012-11-01 00:00:00
abstract：:Although hypogonadism is a risk factor for bone loss and fractures, the different etiopathophysiology and hormonal profile of classical and obesity-induced hypogonadism may lead to differences in musculoskeletal profile. This is a cross-sectional study of hypogonadal men between 40 and 74 years old. Our outcomes inclu...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-017-0316-x
更新日期：2017-12-01 00:00:00
abstract：:The purpose of this study was to examine the influence of exercise on the serum concentrations of intact parathyroid hormone (PTH). Serum PTH and plasma lactate were measured in 15 well-trained men, 9 long-distance runners and 6 fire-fighters, during two running exercises. Test one consisted of 40-minute treadmill run...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00295947
更新日期：1994-04-01 00:00:00
abstract：:Previous observational studies have identified various risk factors associated with the development of osteoporosis, including sex hormone-binding globulin (SHBG). The aim of this study was to determine the potential causal effects of circulating SHBG concentrations on bone mineral density (BMD). Two-sample Mendelian ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-020-00770-8
更新日期：2020-10-17 00:00:00
abstract：:Allogenous demineralized bone matrix (DBM) represents a potential scaffold for bone tissue engineering due to its close relation in structure and function with autologous bone, but its supply is often restricted by donor availability. Thus, an expanded source of human bone is needed. The aim of this study was to evalu...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-008-9159-9
更新日期：2008-09-01 00:00:00
abstract：:To assess the usefulness of the measurement of the os calcis by ultrasound, a method that probably reflects bone quality as well as density, we have studied 54 women with hip fracture of the proximal femur and a control group. Ultrasound evaluation of the os calcis [broadband ultrasound attenuation (BUA), speed of the...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00310252
更新日期：1995-09-01 00:00:00
abstract：:A guidance on the assessment and treatment of postmenopausal women at risk from fractures due to osteoporosis was recently published in Osteoporosis International as a joint effort of the International Osteoporosis Foundation and European Society for Clinical and Economic Aspects of Osteoporosis and Osteoarthritis (Ka...
journal_title：Calcified tissue international
pub_type： 杂志文章,评审
doi：10.1007/s00223-018-00512-x
更新日期：2019-03-01 00:00:00
abstract：:The purpose of this study was to determine whether total serum calcium, parathyroid gland structure, and/or levels of parathyroid hormone, 1,25 and 24,25 DHCC, are altered in pigs with dental and skeletal fluorosis. Eight experimental animals receiving 2 mg F-/kg b.w. per day from age 8-14 months were compared with ei...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02556714
更新日期：1986-04-01 00:00:00
abstract：:Several studies have demonstrated that bone has the power of regeneration and repair. BMPs (bone morphogenetic proteins) are involved in the determination of osteoblast phenotype and bone turnover, therefore genes coding for these proteins, like BMP-4, could be considered potential candidate genes for osteoporosis. We...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223001103
更新日期：2000-07-01 00:00:00
abstract：:The effects of a series of novel phosphonates on the kinetics of mineral development in an ionophore-primed 7:2:1 phosphatidylcholine (PC): dicetylphosphate (DCP): cholesterol (Chol) liposomal model system are reported. When present at 2.5 micromol/liter or 25 micromol/liter concentrations in the solution surrounding ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02509384
更新日期：1996-05-01 00:00:00
abstract：:Aging and sex hormones related changes lead to inflammatory and oxidant conditions, which are involved in the pathogenesis of osteoporosis. Recent studies have suggested that polyphenols may exert a protective effect in such conditions. We assessed the effect of phloridzin (Phlo), a flavonoid exclusively found in appl...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-005-0060-5
更新日期：2005-11-01 00:00:00
abstract：:To determine the relationships among nutrients intake, bone mass, and bone turnover in women we have investigated these issues in a population-based, cross-sectional, observational study in one county in central Sweden. A total of 175 women aged 28-74 at entry to the study were included. Dietary assessment was made by...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00298425
更新日期：1995-08-01 00:00:00
abstract：:Hypophosphatemic rickets (HR) is a group of rare disorders caused by excessive renal phosphate wasting. The purpose of this cross-sectional study of 38 HR patients was to characterize the phenotype of adult HR patients. Moreover, skeletal and endodontic severity scores were defined to assess possible gender difference...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-010-9373-0
更新日期：2010-08-01 00:00:00
abstract：:Measurement of areal bone mineral density (aBMD) in intravertebral subregions may increase the diagnostic sensitivity of dual-energy X-ray absorptiometry (DXA)-derived parameters for vertebral fragility. This study investigated whether DXA-derived bone parameters in vertebral subregions were better predictors of verte...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-014-9866-3
更新日期：2014-08-01 00:00:00
abstract：:The effect of the configuration of porous cylindrical hydroxyapatite (HA) scaffold and laminin preparation of the scaffold on bone formation was estimated. HA scaffolds with a hollow center of 2 or 4 mm in diameter and those without a hollow center were used. The scaffolds were immersed in laminin solution or in cultu...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-008-9157-y
更新日期：2008-08-01 00:00:00
abstract：:The crystallinity of bone mineral at different stages of maturation has been measured by quantitative X-ray diffraction methods. Crystallinity measurements were made on tibial mid-diaphyses from 17-day embryonic chicks, newly-formed periosteal bone from embryonic chicks, and density-fractionated bone from post-hatch c...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02405032
更新日期：1983-01-01 00:00:00
abstract：:Mice deficient in the chloride channel ClC-7, which is likely involved in acidification of the resorption lacuna, display severe osteopetrosis. To fully characterize the osteopetrotic phenotype, the phenotypes of osteoclasts and osteoblasts were evaluated. ClC-7(-/-) mice and their corresponding wild-type littermates ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-008-9185-7
更新日期：2008-12-01 00:00:00
abstract：:Calcaneus bone mineral density (BMD) of 738 Japanese women (605 healthy and 133 with osteoporosis) was measured using single X-ray absorptiometry (SXA). A reference range of calcaneus BMD values for healthy Japanese women was established and the usefulness of this method for screening and diagnosis of osteoporosis was...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900665
更新日期：1999-08-01 00:00:00
abstract：:Prosected vertebrae and phalanges from 32 cadavera were examined using noninvasive methods commonly used for assessment of osteoporosis. Bone measures were then compared with a strength variable obtained by mechanically crushing or breaking the bones. All of the phalangeal density assessment techniques were found to b...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02555150
更新日期：1988-08-01 00:00:00
abstract：:Newly developed fuzzy logic-derived structural parameters were used to characterize trabecular bone architecture in high-resolution magnetic resonance imaging (HR-MRI) of human cadaver calcaneus specimens. These parameters were compared to standard histomorphological structural measures and analyzed concerning perform...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-007-9058-5
更新日期：2007-10-01 00:00:00
abstract：:Roux-en-Y gastric bypass surgery (RYGB) is an effective treatment of morbid obesity, with positive effects on obesity-related complications. The treatment is associated with bone loss, which in turn might increase fracture risk. The aim of this study was to evaluate changes in bone mineral density (BMD) and bone archi...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-015-0091-5
更新日期：2016-03-01 00:00:00
abstract：:Crouzon syndrome is a debilitating congenital disorder involving abnormal craniofacial skeletal development caused by mutations in fibroblast growth factor receptor-2 (FGFR2). Phenotypic expression in humans exhibits an autosomal dominant pattern that commonly involves premature fusion of the coronal suture (craniosyn...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-013-9701-2
更新日期：2013-05-01 00:00:00
abstract：:Arterial calcification leading to increased arterial stiffness, a powerful risk factor for cardiovascular disease, may underlie the association of osteoporosis with cardiovascular disease in postmenopausal women. Osteoprotegerin (OPG), an indirect inhibitor of osteoclastogenesis, may be involved in arterial calcificat...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-008-9153-2
更新日期：2008-08-01 00:00:00
abstract：:The definition of osteoporosis was based for several years on bone mineral density values, which were used by most guidelines for defining treatment thresholds. The availability of tools for the estimation of fracture risk, such as FRAX™ or its adapted Italian version, DeFRA, is providing a way to grade osteoporosis s...
journal_title：Calcified tissue international
pub_type： 社论
doi：10.1007/s00223-013-9748-0
更新日期：2013-09-01 00:00:00
abstract：:SUMMARY. The high variation often observed in the ex vivo fibroblastic-colony forming unit (CFU-f) assay is likely to be due to both biological and experimental variation. To determine whether we could improve experimental methods we developed an alternative method of bone marrow cell (BMC) isolation employing a centr...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900723
更新日期：1999-11-01 00:00:00
abstract：:A simple method for determining histamine and polyamines in various tissues was devised. The method, however, could not be applied to calcified tissues, because the high concentration of Ca2+ in the extract interferes with the chromatographic separation of these amines. By treating the extracts from calcified tissues ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00296220
更新日期：1992-07-01 00:00:00
abstract：:Ipriflavone (i.p.) positively affects bone density in postmenopausal osteoporosis, primarily by inhibiting bone resorption. Using in vitro models of human osteoblast differentiation, we have observed that i.p. and some of its metabolites stimulate the expression of bone sialoprotein, decorin, and type I collagen, and ...
journal_title：Calcified tissue international
pub_type： 杂志文章,评审
doi：10.1007/s002239900378
更新日期：1997-01-01 00:00:00
abstract：:We studied the effect of raloxifene (RAL) on arthritis and bone mineral density (BMD) in rats with collagen-induced arthritis (CIA). Seven-month-old female Sprague-Dawley rats were divided into five groups: rats without CIA (CNT), CIA rats that underwent ovariectomy (OVX) and were treated with RAL (CIA + OVX + RAL), C...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-010-9432-6
更新日期：2011-02-01 00:00:00
