Abstract:
:We investigated the signaling mechanism of stretch-induced cell remodeling in human umbilical vein endothelial cells (HUVECs). Freshly dissociated HUVECs were cultured on an elastic silicon membrane and subjected to uniaxial cyclic stretch (20% in length, 1 Hz). The cells started to change their morphology as early as 15 min after stretch onset, and most cells eventually aligned perpendicularly to the stretch axis within 1 h. This remodeling was dependent on the increase in intracellular calcium concentration ([Ca2+]i) via a Ca(2+)-permeable stretch-activated (SA) channel. During the process of remodeling, extensive rearrangement of stress fibers and focal adhesions was observed, which may be close to the final step in the intracellular signaling cascade. This event was [Ca2+]i-dependent, suggesting the existence of a Ca(2+)-dependent intermediate cascade that links [Ca2+]i to the rearrangement of cytoskeletons and focal adhesions. We found that some proteins, including pp125FAK (focal adhesion kinase) and paxillin, were tyrosine phosphorylated during cyclic stretch in a Ca(2+)-dependent manner. Inhibition of this tyrosine phosphorylation prohibited the stretch-dependent rearrangement of cytoskeletons and focal adhesions as well as the remodeling. Finally the tyrosine kinase src, which could phosphorylate pp125FAK, was found to be activated in a [Ca2+]i-dependent way during stretch. All of the above molecular events were consistently Ca(2+)-dependent, which led us to propose the signaling cascade: SA channel activation-->[Ca2+]i increase-->src activation-->protein tyrosine phosphorylation-->rearrangement of cytoskeletons and focal adhesions-->cell remodeling.
journal_name
Heart Vesselsjournal_title
Heart and vesselsauthors
Sokabe M,Naruse K,Sai S,Yamada T,Kawakami K,Inoue M,Murase K,Miyazu Msubject
Has Abstractpub_date
1997-01-01 00:00:00pages
191-3eissn
0910-8327issn
1615-2573journal_volume
Suppl 12pub_type
杂志文章abstract::An expandable polytetrafluoroethylene (ePTFE) covered stent is generally employed to seal coronary artery perforation. The frequency of ePTFE covered stent use is relatively low; thus, only a handful of studies have reported neointimal coverage and endothelialization inside the deployed ePTFE and clinical time course ...
journal_title:Heart and vessels
pub_type: 杂志文章
doi:10.1007/s00380-017-0964-9
更新日期:2017-06-01 00:00:00
abstract::A 65-year-old man was admitted with chest pain. A diagnosis of spastic angina was made because of symptoms of recurrent anginal attacks associated with ST-segment elevations in the electrocardiogram. A selective coronary arteriogram revealed a 90% diameter narrowing of the proximal left anterior descending coronary ar...
journal_title:Heart and vessels
pub_type: 杂志文章
doi:10.1007/BF02058361
更新日期:1989-01-01 00:00:00
abstract::Peripheral artery disease (PAD) is a risk factor for the development of heart failure and mortality. The serum levels of carboxy-terminal telopeptide of type I collagen (I-CTP), a marker for collagen degradation derived from the extracellular matrix of vascular and myocardial tissue, is reportedly a useful marker for ...
journal_title:Heart and vessels
pub_type: 杂志文章
doi:10.1007/s00380-016-0858-2
更新日期:2017-02-01 00:00:00
abstract::The inotropic effects of 5-hydroxytryptamine (5-HT) on mammalian heart muscles were investigated. 5-HT (10(-8)-10(-3)M) produced increases in the contractile tension of atrial and ventricular muscles isolated from guinea pigs, Japanese monkeys, and humans, but not in rat heart preparations. The maximum percent increas...
journal_title:Heart and vessels
pub_type: 杂志文章
doi:10.1007/BF01744594
更新日期:1996-01-01 00:00:00
abstract::There are few studies regarding the assessment of left ventricular contractile function in patients with atrial fibrillation (AF). The aim of this study was to assess the left ventricular (LV) contractile function, i.e., the end-systolic pressure-volume relation (Ees) and a recently developed LV systolic myocardial st...
journal_title:Heart and vessels
pub_type: 临床试验,杂志文章
doi:10.1007/BF01744589
更新日期:1998-01-01 00:00:00
abstract::Activities of daily living (ADL) are maintained in patients with mild cognitive impairment (MCI), but, in patients with cardiovascular disease, ADL may have already declined. MCI combined with cardiac disease may accentuate the decline of ADL in these patients. Because the relationship between MCI and ADL in patients ...
journal_title:Heart and vessels
pub_type: 杂志文章
doi:10.1007/s00380-019-01437-7
更新日期:2019-12-01 00:00:00
abstract::Chronic kidney disease (CKD) is well known to be associated with an increased incidence of coronary artery disease (CAD). Diabetes mellitus (DM) and hypertension (HTN), both of which are traditional risk factors for CAD, are the two most common causes of CKD. However, the influence of CKD on coronary atherosclerosis i...
journal_title:Heart and vessels
pub_type: 杂志文章
doi:10.1007/s00380-018-01334-5
更新日期:2019-07-01 00:00:00
abstract::A 5-year-old girl developed cardiopulmonary arrest after crying. From the electrocardiogram and echocardiography, a left ventricular noncompaction (LVNC) with long QT syndrome (LQT) was suspected as the cause of the cardiopulmonary arrest, and treatment with a β-blocker and a calcium antagonist was then begun. A genet...
journal_title:Heart and vessels
pub_type: 杂志文章
doi:10.1007/s00380-012-0235-8
更新日期:2013-01-01 00:00:00
abstract::In the present study, we investigated whether gallic acid (GA) can induce death in cultured vascular smooth muscle cells (VSMCs), and whether production of the hydroxyl radical (.OH) is involved in the process of GA action. GA killed cultured VSMCs from rat aorta, in a dosc- and time-dependent manner. Cytoplasmic shri...
journal_title:Heart and vessels
pub_type: 杂志文章
doi:10.1007/s003800070038
更新日期:2000-01-01 00:00:00
abstract::Subintimal or false lumen stent deployment is a rare complication of percutaneous coronary interventions. The most balloon-induced small non-flow limiting dissections heal spontaneously and can be treated medically with close observation if distal coronary flow is not compromised. However, the complex and severe flow-...
journal_title:Heart and vessels
pub_type: 杂志文章
doi:10.1007/s00380-008-1052-y
更新日期:2008-07-01 00:00:00
abstract::The effects of nifedipine on regional dysfunction during pacing-induced ischemia were studied in eight patients with coronary artery disease. Single-plane left ventriculograms were obtained using a high-fidelity micromanometer-tipped catheter in the control and post-pacing periods both before and after pretreatment wi...
journal_title:Heart and vessels
pub_type: 杂志文章
doi:10.1007/BF02073655
更新日期:1985-11-01 00:00:00
abstract::In children, up to 10% of the cases of arterial hypertension may be caused by a renovascular disease. The etiology of this renovascular disease is most of the time due to a fibromuscular dysplasia (FMD), which causes a noninflammatory intimal-medial fibroplasia leading to luminal compromise. Percutaneous transluminal ...
journal_title:Heart and vessels
pub_type: 杂志文章
doi:10.1007/s00380-009-1147-0
更新日期:2009-11-01 00:00:00
abstract::The aim of this study was to examine the efficacy and safety of this novel epicardial circumferential left atrial ablation (CLAA) with pulmonary vein isolation (PVI) in sustained atrial fibrillation (AF). Thirty domestic pigs were divided equally into 3 groups: AF without ablation (AF group), AF with PVI (PVI group), ...
journal_title:Heart and vessels
pub_type: 杂志文章
doi:10.1007/s00380-014-0594-4
更新日期:2015-09-01 00:00:00
abstract::Percutaneous transvenous mitral commissurotomy (PTMC) increases peak oxygen uptake (VO2) chronically, but not acutely, despite early symptomatic improvements. Analysis of transient VO2 responses to submaximal exercise (an exercise regimen more comparable to the patients' daily activities than that provided by maximal ...
journal_title:Heart and vessels
pub_type: 杂志文章
doi:10.1007/BF02911391
更新日期:1995-01-01 00:00:00
abstract::Prolongation of the pulmonary artery potentials (PAPs) in response to short coupling intervals was related to polymorphic QRS configurations during the ventricular tachycardia originating above the pulmonary valve (PA-VT). This prospective study was aimed to investigate the mechanisms of polymorphic changes during the...
journal_title:Heart and vessels
pub_type: 杂志文章
doi:10.1007/s00380-018-1209-2
更新日期:2019-01-01 00:00:00
abstract::Acute kidney injury (AKI) is a common complication after coronary angiography. Early biomarkers of this disease are needed since increase in serum creatinine levels is a late marker. To assess the usefulness of urinary kidney injury molecule-1 (uKIM-1), neutrophil gelatinase-associated lipocalin (uNGAL) and liver-type...
journal_title:Heart and vessels
pub_type: 杂志文章
doi:10.1007/s00380-014-0538-z
更新日期:2015-11-01 00:00:00
abstract::Anatomic continuity between the anterior mitral leaflet and the aortic root may predispose those patients with aortic root pathology to functional changes of the mitral valve without any involvement of this valve. A 34-year-old man presented with aortic valve endocarditis. Transthoracic echocardiograpy showed severe a...
journal_title:Heart and vessels
pub_type: 杂志文章
doi:10.1007/s00380-004-0761-0
更新日期:2005-02-01 00:00:00
abstract::Coronary artery spasm is involved in the pathogenesis of various cardiac disorders. We investigated patients with a history of syncope who underwent elective coronary angiography. We retrospectively analyzed 5781 consecutive patients who had diagnostic or follow-up angiography during a 26-year period. During this peri...
journal_title:Heart and vessels
pub_type: 杂志文章
doi:10.1007/s00380-017-1046-8
更新日期:2018-02-01 00:00:00
abstract::Morphological and functional changes of free arterial grafts in dogs were studied for 3 weeks after implantation and the changes were compared to those in implanted free vein grafts. In the arterial grafts, endothelial cells with abundant pinocytotic vesicles and some cytoplasmic folds were observed by transmission an...
journal_title:Heart and vessels
pub_type: 杂志文章
doi:10.1007/BF01744868
更新日期:1992-01-01 00:00:00
abstract::In a mass chest computed tomography (CT) screening using a mobile helical CT unit, we measured the aortic diameter at three segments to confirm standard values and also attempted to detect any asymptomatic aortic aneurysms. The population screened in the present study consisted of 6971 subjects (3847 men and 3124 wome...
journal_title:Heart and vessels
pub_type: 杂志文章
doi:10.1007/s380-002-8315-1
更新日期:2002-01-01 00:00:00
abstract::In this study, coronary diagnostic parameters, pressure drop coefficient (CDP: ratio of trans-stenotic pressure drop to distal dynamic pressure), and lesion flow coefficient (LFC: ratio of % area stenosis (%AS) to the CDP at throat region), were evaluated to distinguish levels of %AS under varying contractility condit...
journal_title:Heart and vessels
pub_type: 杂志文章
doi:10.1007/s00380-013-0355-9
更新日期:2014-01-01 00:00:00
abstract::Late-acquired stent malapposition (LASM) is a common finding after sirolimus-eluting stent (SES) implantation and may be the cause for late stent thrombosis. Inflammation may play a pivotal role in LASM just as it plays in stent restenosis. We have therefore investigated seven polymorphisms involved in inflammatory pr...
journal_title:Heart and vessels
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00380-010-0046-8
更新日期:2011-05-01 00:00:00
abstract::Adenosine constricted 37 of 58 isolated and perfused monkey coronary arteries, whereas it dilated dog coronary arteries in all cases. The vasoconstriction induced by 0.1-10 micrograms (0.37-37.4 nmol) of adenosine was examined, since adenosine in large doses produced tachyphylaxis. The adenosine-induced vasoconstricti...
journal_title:Heart and vessels
pub_type: 杂志文章
doi:10.1007/BF02058320
更新日期:1990-01-01 00:00:00
abstract::Previous studies suggested that sleep-disordered breathing was associated with cardiovascular diseases such as heart failure (HF). Recently, algorithms of cardiac implantable electronic devices (CIEDs) have been developed to detect advanced sleep apnea (SA); the Apnea Scan (AP Scan) being an example. The purpose of th...
journal_title:Heart and vessels
pub_type: 杂志文章
doi:10.1007/s00380-019-01551-6
更新日期:2020-06-01 00:00:00
abstract::The purpose of this study was to investigate the use of hepatocyte growth factor as a biochemical marker for acute myocardial infarction. Several biochemical markers are used for noninvasive detection of acute myocardial infarction. However, hepatocyte growth factor has not been used previously for this purpose. We me...
journal_title:Heart and vessels
pub_type: 杂志文章
doi:10.1007/BF02766790
更新日期:1997-01-01 00:00:00
abstract::MicroRNAs (miRNAs) are short non-coding RNAs that regulate gene expression. It seems that microRNA-21 (miR-21) and Visfatin, a novel adipocytokine, play roles in inflammation and atherosclerosis. The aim of this study was to investigate the association of miR-21 with Visfatin, inflammation, atherosclerosis and acute c...
journal_title:Heart and vessels
pub_type: 杂志文章
doi:10.1007/s00380-016-0913-z
更新日期:2017-05-01 00:00:00
abstract::Resting bradycardia is an important symptom for early diagnosis of anorexia nervosa (AN) during weight loss, and it improves with body-weight recovery. However, chronotropic incompetence (CI) in exercise is observed in some patients with AN despite amelioration of resting bradycardia in the recovery phase. We examined...
journal_title:Heart and vessels
pub_type: 杂志文章
doi:10.1007/s00380-018-1282-6
更新日期:2019-04-01 00:00:00
abstract::It is known that the heart of an athlete has been physiologically adapted by prolonged training. There are a large number of echocardiographic studies which have focused on left ventricular wall thickness and dilatation, but there are few studies concerning right heart function in the athlete's heart. The aim of this ...
journal_title:Heart and vessels
pub_type: 杂志文章
doi:10.1007/s003800200018
更新日期:2002-07-01 00:00:00
abstract::Left ventricular non-compaction (LVNC) is genetically heterogeneous. It has been previously shown that LVNC is associated with defects in TAZ, DNTA, LDB3, YWHAE, MIB1, PRDM16, and sarcomeric genes. This study was aimed to investigate sarcomeric gene mutations in a Chinese population with LVNC. From 2004 to 2010, 57 un...
journal_title:Heart and vessels
pub_type: 杂志文章
doi:10.1007/s00380-014-0503-x
更新日期:2015-03-01 00:00:00
abstract::We developed a new individually customized contrast-injection protocol for coronary computed tomography (CT) angiography based on the time-attenuation response in a test bolus, and investigated its clinical applicability. We scanned 60 patients with suspected coronary diseases using a 64-detector CT scanner, who were ...
journal_title:Heart and vessels
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00380-013-0338-x
更新日期:2014-03-01 00:00:00