Abstract:
:Human whole saliva was collected at 4 degrees C into a protein inhibitor cocktail to prevent proteolytic degradation. Saliva was clarified by centrifugation and fractionated by immobilized metal affinity chromatography (IMAC) after charging the column with zinc ions. Proteins with an affinity for zinc were eluted from the IMAC column, characterized by sodium dodecylsulphate-polyacrylamide gel electrophoresis, and probed with 65ZnCl2 after electrotransfer on to polyvinylidenefluoride membranes. A zinc-binding protein of approx. 11 kDa was characterized by N-terminal sequencing followed by a FASTA search of Genbank. The first 40 residues were sequenced, of which the first 34 residues were used to conduct a FASTA search and yielded and homology > 97% coding for sequences of mRNA of two proteins, a cystic fibrosis antigen with an M(r) of 10,938 and a calcium-binding inflammatory protein MRP8 with an M(r) of 10,835 expressed in chronic inflammation. The identity of the last 15 residues of the sequence and the likelihood that the protein is secreted via saliva indicates that the 11-kDa protein is the cystic fibrosis antigen, a protein not previously reported in saliva.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Davey HP,Embery G,Creeth JE,Cummins Ddoi
10.1016/s0003-9969(97)00035-6subject
Has Abstractpub_date
1997-12-01 00:00:00pages
861-7issue
12eissn
0003-9969issn
1879-1506pii
S0003-9969(97)00035-6journal_volume
42pub_type
杂志文章abstract::Salivary proteins and glycoproteins that participate in the formation of 2-h in vivo enamel pellicle were determined utilizing polyacrylamide gel electrophoresis [sodium dodecyl sulphate (SDS)-PAGE and anionic PAGE]/Western transfer analyses, and specific radiolabelling/SDS-PAGE fluorography. The sensitivity of these ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(89)90070-8
更新日期:1989-01-01 00:00:00
abstract:OBJECTIVE:Interleukin (IL)-12 is an important cytokine for innate and adaptive immunity. We previously reported that IL-12 inhibits tumour necrosis factor (TNF)-α-mediated osteoclast formation by inducing apoptosis. We also reported that TNF-α plays an important role in mechanical loading-induced osteoclast formation a...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2011.07.006
更新日期:2012-01-01 00:00:00
abstract:OBJECTIVE:To investigate the antibacterial activity of povidone-iodine (PVP-I) on an artificial dual species biofilm of periodontal pathogens. DESIGN:Porphyromonas gingivalis or Fusobacterium nucleatum grown in broth culture was inoculated on polycarbonate membrane (PCM) tissue culture inserts. After incubation for 72...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2011.09.005
更新日期:2012-04-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to investigate the age-related changes in the articular disc, condyle, condylar fibrocartilage, and subchondral bone of the temporomandibular joint (TMJ) in rabbits. METHODS:Female New Zealand white rabbit aged 1, 4, 12, and 32 weeks were obtained. Each age group comprised 5 rabbits...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2019.104639
更新日期:2020-03-01 00:00:00
abstract::It is well accepted that reduced masticatory function induced by a diet with soft physical consistency causes alterations in the craniofacial morphology in growing animals. It is assumed that these alterations are associated with reduced proliferative activity of osteoblasts on the bone surface, indicating a significa...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(00)00057-1
更新日期:2000-10-01 00:00:00
abstract:OBJECTIVE:Acidic diets are advocated as main risk factor for tooth erosion, which could be prevented, or at least controlled, if patients were early advised. It is important to identify, hence, if possible dietary constituents regionally consumed on large scale, such as tucupi, a low-pH yellowish-green color and strong...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2015.10.021
更新日期:2016-01-01 00:00:00
abstract::The classical hydrodynamic theory implicated fluid movement as a transducing mechanism in the production of dental sensitivity. This theory assumes that sensitive dentine must be permeable. Various measurements of dentine permeability are discussed, including: (1) factors that influence diffusive permeation across den...
journal_title:Archives of oral biology
pub_type: 杂志文章,评审
doi:10.1016/0003-9969(94)90191-0
更新日期:1994-01-01 00:00:00
abstract:OBJECTIVE:The DNA oxidation byproduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a well-known biomarker used to evaluate oxidative stress. We previously reported that the generation of reactive oxygen species (ROS) is increased in cultured gingival fibroblasts (GF) from patients with Down syndrome (DS). Thus, the aim of t...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2013.03.017
更新日期:2013-09-01 00:00:00
abstract:OBJECTIVE:Preservation of structural and biochemical properties of the root dentin matrix is crucial to favor healing and regenerative periodontal processes. Aim of this study was to evaluate the biochemical characteristics of collagen and chondroitin sulphate of root dentin surfaces exposed by periodontal disease afte...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2006.07.001
更新日期:2007-01-01 00:00:00
abstract::The effect of chronic mandibular protrusion on the collagenolytic and phosphatase activity of several mandibular bone sites and the condylar cartilage was evaluated. Ninety-three male Sprague-Dawley rats were equally divided into two experimental and one control group. One experimental group wore a protrusive applianc...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(89)90067-8
更新日期:1989-01-01 00:00:00
abstract:OBJECTIVES:The holy grail of biomarker research in periodontology is to develop a high impact diagnostics which have a significant impact on clinical decision-making, patient outcomes and healthcare providers. In the field of periodontal diagnostics, oral fluid-based biomarkers have been studied mainly in the gingival ...
journal_title:Archives of oral biology
pub_type: 杂志文章,评审
doi:10.1016/j.archoralbio.2017.12.022
更新日期:2018-03-01 00:00:00
abstract::Biomechanical loads were varied by feeding diets of different consistencies. One group was fed hard diet for eight weeks, a second soft diet for eight weeks, and a third soft diet for four weeks followed by four weeks of hard diet. In all groups, alkaline-phosphatase activity was localized primarily in cells and extra...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(87)90043-4
更新日期:1987-01-01 00:00:00
abstract::To study the effects of increased occlusal vertical dimension on these kinetics, the actin-filament sliding velocity on masseter myosins in an in vitro motility assay and the ATPase activity of masseter myosins from normal (control) and bite-opened (5.6 mm increase in the vertical dimension for 1 week) guinea-pigs wer...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(97)00038-1
更新日期:1997-07-01 00:00:00
abstract:BACKGROUND:Spondias mombin has been used in folk medicine to treat inflammation in the oral cavity. This study aimed to evaluate the antioxidant and anti-inflammatory activity of S. mombin extract in an oral mucositis experimental model. METHODS:Male hamsters were orally pre-treated with hydroethanolic extract of S. m...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2020.104664
更新日期:2020-03-01 00:00:00
abstract::Substituting ferrets for rats and dogs as animal models for craniofacial research is favourable because of the similarity of many of the ferret's anatomical, metabolic and physiological features to those of man. Other advantages are cost-effectiveness and possibly less ethical controversy. However, information on the ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(02)00043-2
更新日期:2002-08-01 00:00:00
abstract:OBJECTIVE:Enterococcus faecalis is a Gram-positive, facultative anaerobic bacterium that is associated with failed endodontic cases and nosocomial infections. E. faecalis can form biofilms, penetrate dentinal tubules and survive in root canals with scarce nutritional supplies. These properties can make E. faecalis resi...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2016.07.001
更新日期:2016-11-01 00:00:00
abstract:OBJECTIVE:This study aimed to identify candidate genes as potential biomarkers in nasopharyngeal carcinoma (NPC) by bioinformatical analysis. METHODS:Three microarray datasets: GSE32906, GSE15170, GSE53819 were download from public database and analyzed to identify the differentially expressed genes (DEGs) between NPC...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2019.07.003
更新日期:2019-10-01 00:00:00
abstract::The aim of this study was to investigate the age-related developmental changes of taste bud distribution within the subpopulations at different postnatal ages in the mouse oral cavity. Developmental changes of taste bud distribution on the soft palate, fungiform, foliate and circumvallate papillae in the mouse oral ca...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2008.01.005
更新日期:2008-06-01 00:00:00
abstract::Daily intravenous administration of 50 mg/kg cyclosporin A (CsA), after excision of cheek pouch tumors induced with 9,10-dimethyl-1,2-benzanthracene (DMBA), enhanced the incidence of cervical lymph node metastasis to 93%, a significant increase over the rates observed for animals receiving a dosage of 25 mg/kg (43%), ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(92)90143-v
更新日期:1992-01-01 00:00:00
abstract::Exposure to high fluoride concentrations in the immediate environment of the tissue is recognized to result in the post-translational modification of non-collagenous dentine extracellular matrix (ECM) components, potentially altering dentine mineralization. However, less is known about the effects of fluoride exposure...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(02)00160-7
更新日期:2003-01-01 00:00:00
abstract::Peptidoglycans and cells walls from Actinomyces viscosus, Staphylococcus aureus, and group A streptococcus were compared for their relative abilities to activate the alternate complement pathway (ACP). On the dry-weight basis, the peptidoglycan from A. viscosus was 3.5 times more active than group A streptococcal pept...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(83)90066-3
更新日期:1983-01-01 00:00:00
abstract:OBJECTIVE:Tenascin is a large extracellular matrix glycoprotein that plays specific role in cell matrix interaction. This protein is mainly attracted because of its oncofetal predominance expression at epithelial-mesenchymal interaction and also been associated with inflammatory response. Thus the aim was to study the ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2010.11.020
更新日期:2011-07-01 00:00:00
abstract::The first indication of cartilage formation was detected on day 14.5 of pregnancy in close connection to the ossifying mandible. A separated blastema was never observed. Thus the findings support the concept that the condylar cartilage develops from already differentiated cells, not from primary undifferentiated mesen...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(95)00105-0
更新日期:1996-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Dental pulp repair is a common process triggered by microbial and mechanical challenges. Matricellular modulators, such as periostin, are key for extracellular matrix stability and tissue healing. In the scope of the dental pulp, periostin expression has been reported during development and act...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2015.02.008
更新日期:2015-05-01 00:00:00
abstract::Degradation of phenylalanine and leucine by resting cells of Eubacterium brachy ATCC 33089 were studied under strict anaerobic conditions. The effects of metronidazole and air on the metabolism were also studied. The organism principally produced phenylpropionate and isocaproate from phenylalanine and leucine, respect...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(94)90080-9
更新日期:1994-11-01 00:00:00
abstract:UNLABELLED:Adaptive remodelling of the mandibular condyle in response to mandibular advancement is the mechanism exploited by orthodontic forward displacement devices. OBJECTIVE:This work investigated the expression of collagens, matrix metalloproteinases and vascular endothelial growth factor during this process. DE...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2011.09.017
更新日期:2012-06-01 00:00:00
abstract:OBJECTIVE:Few clinical techniques are available for the description of mechano-nociceptive function in human periodontal tissues. The objective of this study was to test a new technique for assessment of periodontal mechano-nociceptive function by direct application of controlled forces to the teeth and quantify site, ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2016.07.012
更新日期:2016-11-01 00:00:00
abstract::Although most cases of cleft lip and palate are free of other developmental defects, children with isolated cleft lip/palate are at enhanced risk of delayed growth and reduced final size. Three variables were assessed in the permanent dentition away from the cleft site: congenital absence by tooth type (which ranged f...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(90)90210-2
更新日期:1990-01-01 00:00:00
abstract:OBJECTIVES:The initiation of the pain sensation experienced following the thermal stimulation of dentine has been correlated with fluid flow in the dentinal tubules. There may be other mechanisms. METHODS:This study examines this possibility using a mathematical model to simulate the temperature and thermal stress dis...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2011.02.011
更新日期:2011-09-01 00:00:00
abstract:OBJECTIVES:Dental evolution affects the geometry of the tooth, but the adaptive relevance of these changes is related to tooth sharpness, complexity, and relief (topography). On a set of laboratory mice, we assessed how wear related to age and food consistency affected molar geometry and topography. DESIGN:Three group...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2017.04.028
更新日期:2017-09-01 00:00:00