Abstract:
:A possible link between protein kinase C (PKC) and P-glycoprotein (P-gp)-mediated-multidrug resistance (MDR) was assumed from studies on MDR cell lines selected in vitro. The functional relevance of PKC for the MDR phenotype remains unclear, and the involvement of a particular PKC isozyme in clinically occurring drug resistance is not known. Recently, we have demonstrated significant correlations between the expression levels of the PKC eta isozyme and the MDR1 or MRP (multidrug resistance-associated protein) genes in blasts from patients with acute myelogenous leukaemia (AML) and in ascites cell aspirates from ovarian cancer patients. To extend these findings to further types of human tumours we analysed specimens from 64 patients with primary breast cancer for their individual expression levels of several MDR-associated genes (MDR1, MRP, LRP (lung cancer resistance-related protein), topoisomerase (Topo) II alpha/IIbeta, cyclin A and the PKC isozyme genes (alpha, beta1, beta2, eta, theta, and mu) by a cDNA-PCR approach. We found significantly enhanced mean values for MRP, LRP and PKC eta gene expression, but significantly decreased Topo II alpha and cyclin A gene expression levels in G2 tumours compared with G3. Remarkably, significant positive correlations between the MDR1, MRP or LRP gene expression levels and PKC eta were determined: MDR1/PKC eta (rs = +0.6451, P < 0.0001) n = 62; MRP/PKC eta (rs = +0.5454, P < 0.0001) n = 63; LRP/PKC eta (rs = +0.5436, P < 0.0001) n = 62; MRP/LRP (rs = +0.7703, P < 0.0001) and n = 62, MDR1/MRP (rs = +0.5042, P < 0.0001) n = 62. Our findings point to the occurrence of a multifactorial MDR in the clinics and to PKC eta as a possible key regulatory factor for up-regulation of a series of MDR-associated genes in different types of tumours.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Beck J,Bohnet B,Brügger D,Bader P,Dietl J,Scheper RJ,Kandolf R,Liu C,Niethammer D,Gekeler Vdoi
10.1038/bjc.1998.13subject
Has Abstractpub_date
1998-01-01 00:00:00pages
87-91issue
1eissn
0007-0920issn
1532-1827journal_volume
77pub_type
杂志文章abstract::In vivo DNA damage and repair was induced by nicotinamide (NAM) in adenotype 12 virus-induced mouse sarcoma A12B3 and sarcoma F inoculated into CBA mice. DNA damage, NAM and NAD concentrations were measured after in vivo exposure to NAM, in tumours and spleens by alkaline elution and by HPLC analysis. Our results indi...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1996.367
更新日期:1996-08-01 00:00:00
abstract::Immunofluorescent assessment of hormone receptors in melanocytic tumours is quite feasible without loss of diagnostic material, in contrast to the impracticability of quantitative biochemical assays. Using this method, oestrogen receptors were demonstrated in 4/6, and progesterone receptors in 3/5 patients with metast...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1981.95
更新日期:1981-05-01 00:00:00
abstract:BACKGROUND:A frequent mechanism of acquired multidrug resistance in human cancers is overexpression of ATP-binding cassette transporters such as the Multi-Drug Resistance Protein 1 (MDR-1). Nutlin-3, an MDM2-p53 antagonist, has previously been reported to be a competitive MDR-1 inhibitor. METHODS:This study assessed w...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2014.325
更新日期:2014-08-12 00:00:00
abstract::In some clinical situations the endogenous production of glutamine may be insufficient to maintain optimal tissue structure and function such that glutamine becomes a conditionally essential amino acid. Studies in laboratory animals have demonstrated that glutamine supplementation can reduce the incidence and severity...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1038/bjc.1994.385
更新日期:1994-10-01 00:00:00
abstract::Dipyridamole has been shown to enhance the in vitro activity of antimetabolite anticancer drugs through the inhibition of nucleoside transport. However, the clinical potential of dipyridamole has not been realized because of the avid binding of the drug to the plasma protein alpha1-acid glycoprotein (AGP). Dipyridamol...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6690591
更新日期:1999-08-01 00:00:00
abstract::Pulmonary metastasectomy is widely and increasingly practiced in the belief that this intervention can cure patients with colorectal cancer, and that without it few survive 5 years. No good evidence exists supporting such convictions, indeed recent trial results challenge them. What evidence underpins this acceptance ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/s41416-020-0927-2
更新日期:2020-08-01 00:00:00
abstract::Immunoreactive and bioassayable plasma fibronectin (opsonic α(2) surface-binding (SB) glycoprotein) was measured during experimental Sarcoma-180 tumour growth in mice. Male C57BL/6 mice were challenged s.c. with 2 × 10(6) viable Sarcoma-180 tumour cells and evaluated sequentially in parallel with saline-injected contr...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1980.174
更新日期:1980-06-01 00:00:00
abstract::The levels of CA 242, a new tumour marker of carbohydrate nature, were measured in sera of 185 patients with malignancies of the digestive tract and of 123 patients with benign digestive tract diseases. High percentages of elevated CA 242 levels (greater than 20 U ml-1) were recorded in patients with pancreatic and bi...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1991.146
更新日期:1991-04-01 00:00:00
abstract::HER2/neu-derived peptides inducing MHC class II-restricted CD4+ T helper lymphocyte (Th) responses, although critical for tumour rejection, are not thoroughly characterized. Here, we report the generation and characterization of CD4+ T cell clones specifically recognizing a HER-2/neu-derived peptide (776-788) [designa...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1054/bjoc.2001.2089
更新日期:2001-11-16 00:00:00
abstract::Electrochemotherapy is an anti-tumour treatment that utilizes locally delivered electric pulses to increase cytotoxicity of chemotherapeutic drugs. The aim of our study was to determine whether anti-tumour effectiveness of electrochemotherapy with cisplatin is a consequence of increased plasma membrane permeability ca...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6690222
更新日期:1999-03-01 00:00:00
abstract::Aminoglutethimide without glucocorticoid has been shown to be a clinically effective treatment for postmenopausal breast cancer in low dosage (250 mg day-1). The mechanism of action of this approach is thought to be the inhibition of peripheral aromatase, the enzyme which converts androstenedione to oestrone. The acti...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1985.144
更新日期:1985-07-01 00:00:00
abstract:BACKGROUND:In the coBRIM study, cobimetinib plus vemurafenib (C+V) significantly improved survival outcomes vs placebo and vemurafenib (P+V) in patients with advanced/metastatic BRAFV600-mutated melanoma. An analysis of health-related quality of life (HRQOL) from coBRIM is reported. METHODS:Patients completing the Eur...
journal_title:British journal of cancer
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1038/bjc.2017.488
更新日期:2018-03-20 00:00:00
abstract::The reactivation of U.V.-irradiated adenovirus 2 in HeLa cells is enhanced 8-9 fold if the cells are given a brief hyperthermic shock before infection. Maximum reactivation is achieved by heating for 10 min at 45.5 degrees C and with a delay of 36 h between heating and infection. The induction process requires protein...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1984.32
更新日期:1984-02-01 00:00:00
abstract::Epidemiologic evidence has recently identified an association between an endemic outbreak of pleural and peritoneal mesothelioma in the Urgup region of Turkey and exposure to zeolite fibres. This malignancy is usually associated with exposure to asbestos dusts whose mineralogical characteristics differ from those of z...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1986.158
更新日期:1986-07-01 00:00:00
abstract::The association between Ki-ras mutations and proliferation activity was investigated in a comprehensive series of biliary tract carcinomas (BTCs). We precisely microdissected samples of tissue from paraffin-embedded sections of 77 BTCs including 22 intrahepatic cholangiocarcinomas (ICCs), 36 extrahepatic cholangiocarc...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1996.459
更新日期:1996-09-01 00:00:00
abstract:BACKGROUND:Epstein-Barr virus-positive post-transplant lymphoproliferative disease (PTLD) is a potentially lethal complication of iatrogenic immunosupression after transplantation. Predicting the development of PTLD allowing early and effective intervention is therefore of importance. Polymorphisms within cytokine gene...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6605278
更新日期:2009-09-15 00:00:00
abstract::The large numbers of papillary thyroid carcinomas that have occurred in those exposed to high levels of short-lived isotopes in fallout after Chernobyl provide a unique opportunity to correlate latency and tumour biology. We show that short latency is associated with tumours with a phenotype that is significantly less...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6601860
更新日期:2004-06-01 00:00:00
abstract::The inhibitor of apoptosis protein, XIAP, is frequently overexpressed in chemoresistant human tumours. An antisense oligonucleotide (AEG 35156/GEM 640) that targets XIAP has recently entered phase I trials in the UK. Method validation data are presented on three pharmacodynamic assays that will be utilised during this...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6602363
更新日期:2005-02-14 00:00:00
abstract::Male and female Swiss strain mice were put on a 0.03% thioacetamide diet at the age of 2 months. Control mice were kept on a stock diet. Control and treated mice were killed at the ages of 6, 9, 13 and 17 months. Progressive morphological, histological and biochemical alterations in the liver and tumour tissue of the ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1970.60
更新日期:1970-09-01 00:00:00
abstract::Epstein-Barr virus (EBV) expression was investigated by immunohistochemistry (latent membrane protein 1 [LMP-1]) and in situ hybridization (EBV encoded RNA [EBER]) in biopsies from 95 patients with untreated Hodgkin's disease (HD). Tumour EBV status was related to EBV antibody titres, spontaneous and concanavalin A in...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6690827
更新日期:1999-12-01 00:00:00
abstract::Diagnosis of hereditary non-polyposis colorectal cancer (HNPCC) is currently based on phenotypical analysis of an expanded pedigree. Diagnostic guidelines ('Amsterdam criteria') proposed by the International Collaborative Group on HNPCC are often too stringent for use with small families. There is also the possibility...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1995.508
更新日期:1995-11-01 00:00:00
abstract::This single-centre phase I trial was designed to determine the maximum tolerated dose of irinotecan and the recommended dose to use in combination with a fixed dose of 5-fluorouracil (5-FU) administered as a protracted venous infusion, for the first-line treatment of metastatic colorectal cancer (CRC). Tolerability an...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章
doi:10.1038/sj.bjc.6602173
更新日期:2004-10-18 00:00:00
abstract::High experienced continuity of care in patients with cancer is associated with lower needs for care, better quality of life and better psychological outcomes. We developed and evaluated an intervention to improve experienced continuity. The intervention, consisted of (1) a 17-item patient-completed continuity assessme...
journal_title:British journal of cancer
pub_type: 杂志文章,随机对照试验
doi:10.1038/sj.bjc.6604836
更新日期:2009-01-27 00:00:00
abstract::The x(c)(-) cystine transporter enhances biosynthesis of glutathione, a tripeptide thiol important in drug resistance and cellular defense against oxidative stress, by enabling cellular uptake of cystine, a rate-limiting precursor. Because it is known to regulate glutathione levels and growth of various cancer cell ty...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6604485
更新日期:2008-08-05 00:00:00
abstract::The UK National Case-Control Study Group has examined the relationship between smoking (both own smoking and passive), alcohol consumption and caffeine consumption and the risk of breast cancer. A total of 755 women with breast cancer diagnosed before the age of 36, each with an age-matched general population control,...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1994.258
更新日期:1994-07-01 00:00:00
abstract::Tumour angiogenesis is a complex multistep process regulated by a number of angiogenic factors. One such factor, platelet-derived endothelial cell growth factor has recently been shown to be thymidine phosphorylase (TP). TP catalyses the reversible phosphorylation of thymidine to deoxyribose-1-phosphate and thymine. A...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1996.49
更新日期:1996-02-01 00:00:00
abstract::To evaluate whether green tea consumption provides protection against stomach cancer death, relative risks were calculated using Cox proportional hazards regression analysis in the Japan Collaborative Study for Evaluation of Cancer Risk, sponsored by the Ministry of Health and Welfare (JACC Study). The study was based...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6600487
更新日期:2002-07-29 00:00:00
abstract::We have investigated the effects of 50 min whole-body hyperthermia (WBH; 15 min equilibration followed by 41 degrees C for 35 min) on the toxicity and pharmacokinetics of the radiosensitizer Ro 03-8799 in mice. WBH markedly reduced Ro 03-8799 LD50/7d from 779 to 259 micrograms g-1 (P less than 0.001). Pharmacokinetics...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1987.96
更新日期:1987-05-01 00:00:00
abstract::Emerging evidences suggest the existence of a new mode of epidermal growth factor receptor (EGFR) signalling pathway in which activated EGFR undergoes nuclear translocalization and subsequently regulates gene expression and potentially mediates other cellular processes. This signalling route is distinct from the bette...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::Using bivariate flow cytofluorometry, we have determined the nuclear DNA distribution and the expression of the p21 protein (coded by the Ha-ras oncogene) in the bone marrow (BM) cells of five solid tumour patients having histologically normal BM and in those of 57 patients with plasma cell dyscrasia (28 with monoclon...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1990.379
更新日期:1990-11-01 00:00:00