当前位置： SCI文献检索 > JOURNAL OF HEPATOLOGY期刊下所有文献 > Influence of 48 hours of cold storage in University of Wisconsin organ preservation solution on metabolic capacity of rat hepatocytes.

Influence of 48 hours of cold storage in University of Wisconsin organ preservation solution on metabolic capacity of rat hepatocytes.

Abstract：

BACKGROUND/AIMS:Suspensions of isolated hepatocytes are a valuable tool to study liver functions. For optimal use of the isolated hepatocytes, methods are needed to preserve the hepatocytes while maintaining their viability, metabolic and transport functions. Until now, little has been known about the maintenance of the drug metabolism capacity and energy state, measured by the so-called energy charge (ATP+1/2ADP)/(ATP+ADP+AMP), in hepatocytes after storage in University of Wisconsin cold storage solution (UW). Consequently, we investigated whether UW, originally designed to preserve organs for transplantation, was suitable for preservation of isolated rat hepatocytes with respect to the maintenance of drug metabolism and levels of energy-rich substrates. METHODS:Viability of the isolated rat hepatocytes was determined by trypan blue exclusion, ATP content and energy charge after 24 and 48 h of storage in UW at 0 degrees C. Phase I and II metabolic functions of the cells were studied by measuring the cytochrome P450 content and the metabolic rate of lidocaine and 7-ethoxycoumarin. RESULTS:During 48 h of storage of hepatocytes in UW both phase I and phase II metabolism are preserved at control levels. After storage, the viability of the hepatocytes was not changed significantly, and the cells maintained proper cellular ATP content and overall energy charge. CONCLUSIONS:These results imply that hepatocytes from a single isolation can be stored in UW solution and used for metabolism experiments for 3 consecutive days, allowing a reduction in the use of experimental animals.

journal_name

J Hepatol

journal_title

Journal of hepatology

authors

Olinga P,Merema M,Slooff MJ,Meijer DK,Groothuis GM

doi

10.1016/s0168-8278(97)80091-8

subject

Has Abstract

pub_date

1997-10-01 00:00:00

pages

738-43

issue

4

eissn

0168-8278

issn

1600-0641

pii

S0168-8278(97)80091-8

journal_volume

27

pub_type

杂志文章

相关文献

JOURNAL OF HEPATOLOGY文献大全
  • Expression of MAGE, GAGE and BAGE genes in human liver diseases: utility as molecular markers for hepatocellular carcinoma.

    abstract：BACKGROUND/AIMS:The MAGE, GAGE and BAGE genes encode tumor antigens recognized by autologous cytotoxic T lymphocytes. The aim of this study was to evaluate the possibility of using these genes as molecular markers and as the targets of specific immunotherapy for human hepatocellular carcinoma (HCC). METHODS:The expres...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/s0168-8278(00)80223-8

    authors： Kobayashi Y,Higashi T,Nouso K,Nakatsukasa H,Ishizaki M,Kaneyoshi T,Toshikuni N,Kariyama K,Nakayama E,Tsuji T

    更新日期：2000-04-01 00:00:00

  • The lung in patients with cirrhosis.

    abstract：:To summarize, nowadays it seems clear that in patients with cirrhosis: (a) the pulmonary circulation is usually markedly dilated. (b) This vasodilation is due to a loss of vascular tone and it is characterized by a poor (or even absent) vascular reactivity in front of the hypoxic stimulus. (c) This abnormal behaviour ...

    journal_title：Journal of hepatology

    pub_type： 杂志文章,评审

    doi：10.1016/0168-8278(90)90061-u

    authors： Agustí AG,Roca J,Bosch J,Rodriguez-Roisin R

    更新日期：1990-03-01 00:00:00

  • Glucagon-like peptide-1 reduces hepatic lipogenesis via activation of AMP-activated protein kinase.

    abstract：BACKGROUND & AIMS:Glucagon-like peptide-1 (GLP-1), a gut-derived peptide degraded by dipeptidyl peptidase-4 (DPP4), stimulates insulin secretion in response to nutrients, yet its direct effect on the liver is controversial. We investigated the effects of GLP-1 on hepatic fat and glucose metabolism and elucidated its me...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2010.09.032

    authors： Ben-Shlomo S,Zvibel I,Shnell M,Shlomai A,Chepurko E,Halpern Z,Barzilai N,Oren R,Fishman S

    更新日期：2011-06-01 00:00:00

  • The non-structural 5A protein of hepatitis C virus exhibits genotypic differences in interferon antagonism.

    abstract：BACKGROUND/AIMS:Patients infected with hepatitis C virus (HCV) genotype 2 or 3 usually respond better to interferon (IFN) treatment than those infected with genotype 1. In this study, we investigated whether the non-structural 5A protein (NS5A) of HCV genotypes 1 and 2 (1b-NS5A and 2a-NS5A, respectively) exerted differ...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2008.06.030

    authors： Tsai YH,Kuang WF,Lu TY,Kao JH,Lai MY,Liu CJ,Chen PJ,Hwang LH

    更新日期：2008-12-01 00:00:00

  • Hepatopulmonary syndrome in candidates for liver transplantation.

    abstract：BACKGROUND:Hepatopulmonary syndrome (HPS) has been defined as a clinical triad, including chronic liver disease, gas exchange defects (increased alveolar-arterial PO2 difference irrespective of the presence of arterial hypoxemia), and widespread intrapulmonary vascular dilatations. We determined the incidence and the c...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/s0168-8278(00)00108-2

    authors： Martínez GP,Barberà JA,Visa J,Rimola A,Paré JC,Roca J,Navasa M,Rodés J,Rodriguez-Roisin R

    更新日期：2001-05-01 00:00:00

  • Heterogeneity of hepatitis B virus C-gene sequences: implications for amplification and sequencing.

    abstract：:Occasionally direct sequencing of amplified hepatitis B virus DNA leads to weak signals on autoradiograms. Using amplified C-gene sequences we investigated whether this is due to sequence heterogeneity of virus populations and use of inappropriate primers for direct sequencing. High C-gene sequence heterogeneity (poin...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/s0168-8278(05)80009-1

    authors： Miska S,Günther S,Vassilev M,Meisel H,Pape G,Will H

    更新日期：1993-04-01 00:00:00

  • Applicability of adult-to-adult living donor liver transplantation.

    abstract：BACKGROUND/AIMS:The applicability of adult-to-adult living donor liver transplantation has not been established yet. We report the first data in a European center of the process leading to this procedure from the first moment the patients were informed about it. METHODS:In phase 1 of the process, 121 adult patients en...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2005.02.016

    authors： Rimola A,Llovet JM,Navasa M,Bruix J,Londoño MC,Fuster J,García-Valdecasas JC

    更新日期：2005-07-01 00:00:00

  • Quality of life and cognitive function in hepatitis C at different stages of liver disease.

    abstract：BACKGROUND/AIMS:Hepatitis C has been associated with a decrease in quality of life and with neurological abnormalities. The aim of our study was to investigate the relationship between quality of life and cognitive function. METHODS:Quality of life, clinical variables and neuropsychological function were evaluated in ...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/s0168-8278(03)00189-2

    authors： Córdoba J,Flavià M,Jacas C,Sauleda S,Esteban JI,Vargas V,Esteban R,Guardia J

    更新日期：2003-08-01 00:00:00

  • Ultrarapid hepatitis C virus clearance by daily high-dose interferon in non-responders to standard therapy.

    abstract：BACKGROUND/AIMS:To analyze the kinetics of the hepatitis C virus and the patterns of resistance to interferon alpha, we assessed HCV RNA levels early during retreatment with high-dose interferon in patients who did not respond to standard treatment. METHODS:Eleven non-responders to previous therapy with 3-6 MU interfe...

    journal_title：Journal of hepatology

    pub_type： 临床试验,杂志文章

    doi：10.1016/s0168-8278(98)80343-7

    authors： Bekkering FC,Brouwer JT,Leroux-Roels G,Van Vlierberghe H,Elewaut A,Schalm SW

    更新日期：1998-06-01 00:00:00

  • IL28 variation affects expression of interferon stimulated genes and peg-interferon and ribavirin therapy.

    abstract：BACKGROUND & AIMS:Common genetic variation within the IL28 locus has been found to influence the effect of peg-interferon and ribavirin combination therapy against chronic hepatitis C virus (HCV) infection. Expression of IL28 in peripheral blood cells has been reported to be higher in patients with IL28 SNP genotypes a...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2010.09.019

    authors： Abe H,Hayes CN,Ochi H,Maekawa T,Tsuge M,Miki D,Mitsui F,Hiraga N,Imamura M,Takahashi S,Kubo M,Nakamura Y,Chayama K

    更新日期：2011-06-01 00:00:00

  • Actin is a target antigen of anti-neutrophil cytoplasmic antibodies (ANCA) in autoimmune hepatitis type-1.

    abstract：BACKGROUND/AIM:Anti-neutrophil cytoplasmic antibodies (ANCA) are a group of autoantibodies first associated with Wegener's granulomatosis and microscopic polyangiitis. The significance of ANCA in autoimmune hepatitis remains uncertain; the nature of the antigen or antigens has not been defined yet. The purpose of this ...

    journal_title：Journal of hepatology

    pub_type： 临床试验,杂志文章

    doi：10.1016/s0168-8278(97)80007-4

    authors： Orth T,Gerken G,Kellner R,Meyer zum Büschenfelde KH,Mayet WJ

    更新日期：1997-01-01 00:00:00

  • Non-hypervascular hypointense nodules detected by Gd-EOB-DTPA-enhanced MRI are a risk factor for recurrence of HCC after hepatectomy.

    abstract：BACKGROUND & AIMS:The gadolinium-ethoxybenzyl-diethylenetriamine pentaacetic acid (Gd-EOB-DTPA)-enhanced magnetic resonance imaging (MRI) often depicts non-hypervascular hypointense hepatic nodules during the hepatobiliary phase in patients with hepatocellular carcinoma (HCC). It is unclear whether the presence of thes...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2013.01.030

    authors： Toyoda H,Kumada T,Tada T,Niinomi T,Ito T,Sone Y,Kaneoka Y,Maeda A

    更新日期：2013-06-01 00:00:00

  • Toxicity of low dose azathioprine and 6-mercaptopurine in rat hepatocytes. Roles of xanthine oxidase and mitochondrial injury.

    abstract：BACKGROUND/AIMS:To study effects of pharmacologic concentrations of azathioprine and 6-mercaptopurine (6-MP) on rat hepatocytes. METHODS:Hepatocytes cultured on matrigel were incubated with azathioprine or 6-MP; effects of putative protective agents were studied. Viability (LDH leakage), reduced (GSH) and oxidized glu...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2003.11.024

    authors： Tapner MJ,Jones BE,Wu WM,Farrell GC

    更新日期：2004-03-01 00:00:00

  • Statin use and the risk of hepatocellular carcinoma in patients at high risk: A nationwide nested case-control study.

    abstract：BACKGROUND & AIMS:Statins are widely used to treat hypercholesterolemia. Statins may prevent hepatocellular carcinoma (HCC), but have not yet been fully studied, particularly in patients at high risk. Therefore, we investigated the risk of HCC after statin use in the whole general population and evaluated the effects o...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2017.10.018

    authors： Kim G,Jang SY,Nam CM,Kang ES

    更新日期：2018-03-01 00:00:00

  • 'Lupus' anticoagulant and thrombosis of the hepatic veins (Budd-Chiari syndrome). Report of three patients and review of the literature.

    abstract：:Three patients with Budd-Chiari syndrome in association with the 'lupus' anticoagulant are described. This anticoagulant was looked for in our patients with an otherwise idiopathic hepatic venous thrombosis because of the presence of a pronounced thrombocytopenia, a prolonged activated partial thromboplastin time or a...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/s0168-8278(86)80151-9

    authors： Van Steenbergen W,Beyls J,Vermylen J,Fevery J,Marchal G,Desmet V,De Groote J

    更新日期：1986-01-01 00:00:00

  • Non-invasive stratification of hepatocellular carcinoma risk in non-alcoholic fatty liver using polygenic risk scores.

    abstract：BACKGROUND & AIMS:Hepatocellular carcinoma (HCC) risk stratification in individuals with dysmetabolism is a major unmet need. Genetic predisposition contributes to non-alcoholic fatty liver disease (NAFLD). We aimed to exploit robust polygenic risk scores (PRS) that can be evaluated in the clinic to gain insight into t...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2020.11.024

    authors： Bianco C,Jamialahmadi O,Pelusi S,Baselli G,Dongiovanni P,Zanoni I,Santoro L,Maier S,Liguori A,Meroni M,Borroni V,D'Ambrosio R,Spagnuolo R,Alisi A,Federico A,Bugianesi E,Petta S,Miele L,Vespasiani-Gentilucci U,Anstee

    更新日期：2020-11-25 00:00:00

  • Causal relationships between NAFLD, T2D and obesity have implications for disease subphenotyping.

    abstract：BACKGROUND & AIMS:Non-alcoholic fatty liver disease (NAFLD), type 2 diabetes (T2D) and obesity are epidemiologically correlated with each other but the causal inter-relationships between them remain incompletely understood. We aimed to explore the causal relationships between the 3 diseases. METHODS:Using both UK Biob...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2020.03.006

    authors： Liu Z,Zhang Y,Graham S,Wang X,Cai D,Huang M,Pique-Regi R,Dong XC,Chen YE,Willer C,Liu W

    更新日期：2020-08-01 00:00:00

  • Hepatic immunohistochemical staining with a monoclonal antibody against HCV-E2 to evaluate antiviral therapy and reinfection of liver grafts in hepatitis C viral infection.

    abstract：BACKGROUND/AIMS:A simple and reproducible hepatic immunohistochemical staining (IHS) for hepatitis C virus (HCV) is not available. We aimed to validate hepatic IHS with monoclonal antibody (Mab) IG222, directed against the HCV-envelope 2 (E2) protein. METHODS:A three-step indirect immunoperoxidase method was used for ...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/s0168-8278(02)00389-6

    authors： Verslype C,Nevens F,Sinelli N,Clarysse C,Pirenne J,Depla E,Maertens G,van Pelt J,Desmet V,Fevery J,Roskams T

    更新日期：2003-02-01 00:00:00

  • Hepatitis C virus infection and chronic kidney disease: Time for reappraisal.

    abstract：:Hepatitis C virus (HCV) infection is associated with tremendous morbidity and mortality due to liver complications. HCV infection is also associated with many extrahepatic manifestations including cardiovascular diseases, glucose metabolism impairment, cryoglobulinemia vasculitis, B cell non-Hodgkin lymphoma and chron...

    journal_title：Journal of hepatology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jhep.2016.06.011

    authors： Cacoub P,Desbois AC,Isnard-Bagnis C,Rocatello D,Ferri C

    更新日期：2016-10-01 00:00:00

  • Biochemical and histological effects of 26 weeks of glycyrrhizin treatment in chronic hepatitis C: a randomized phase II trial.

    abstract：BACKGROUND/AIMS:Phase I/II studies of 4 weeks duration have confirmed the ALT lowering effect of glycyrrhizin in Western chronic hepatitis C patients. Our aim was to determine the dose frequency of glycyrrhizin required to maintain the ALT response beyond 4 weeks and evaluate its effect on liver histology and quality o...

    journal_title：Journal of hepatology

    pub_type： 杂志文章,多中心研究,随机对照试验

    doi：10.1016/j.jhep.2006.05.015

    authors： Orlent H,Hansen BE,Willems M,Brouwer JT,Huber R,Kullak-Ublick GA,Gerken G,Zeuzem S,Nevens F,Tielemans WC,Zondervan PE,Lagging M,Westin J,Schalm SW

    更新日期：2006-10-01 00:00:00

  • Indication of liver transplantation in severe alcoholic liver cirrhosis: quantitative evaluation and optimal timing.

    abstract：BACKGROUND/AIMS:The aim of our study was to evaluate the proportion of patients with severe alcoholic cirrhosis who would need orthotopic liver transplantation (OLT) and to determine the optimal delay to evaluate an abstinent patient for transplantation. METHODS:Survival without OLT, improvement in liver function and ...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/s0168-8278(01)00228-8

    authors： Veldt BJ,Lainé F,Guillygomarc'h A,Lauvin L,Boudjema K,Messner M,Brissot P,Deugnier Y,Moirand R

    更新日期：2002-01-01 00:00:00

  • Myofibroblasts and hepatocellular carcinoma: an in vivo and in vitro study.

    abstract：BACKGROUND/AIMS:The number of perisinusoidal myofibroblasts has been shown to be increased in hepatocellular carcinoma, as compared to cirrhosis. This increase might suggest a cooperative relationship between tumour cells and myofibroblasts. To assess this relationship, we undertook: (a) an immunohistochemical study to...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/s0168-8278(98)80186-4

    authors： Nhieu JT,Brochériou I,Préaux AM,Mallat A,Cherqui D,Zafrani ES,Mavier P

    更新日期：1998-07-01 00:00:00

  • Living donor vs. deceased donor liver transplantation for patients with hepatitis C virus-related diseases.

    abstract：BACKGROUND & AIMS:Living donor liver transplantation (LDLT) provides a timely alternative to deceased donor liver transplantation (DDLT) for patients with hepatitis C virus-related (HCV-related) diseases in the circumstances of severe organ dearth. However, the patient and graft outcomes, and recurrence of HCV after LD...

    journal_title：Journal of hepatology

    pub_type： 杂志文章,meta分析,评审

    doi：10.1016/j.jhep.2012.07.015

    authors： Hu A,Liang W,Zheng Z,Guo Z,He X

    更新日期：2012-12-01 00:00:00

  • Toll-like receptor 3 downregulation is an escape mechanism from apoptosis during hepatocarcinogenesis.

    abstract：BACKGROUND & AIMS:Low levels of toll-like receptor 3 (TLR3) in patients with hepatocellular carcinoma (HCC) are associated with poor prognosis, primarily owing to the loss of inflammatory signaling and subsequent lack of immune cell recruitment to the liver. Herein, we explore the role of TLR3-triggered apoptosis in HC...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2019.05.031

    authors： Bonnin M,Fares N,Testoni B,Estornes Y,Weber K,Vanbervliet B,Lefrançois L,Garcia A,Kfoury A,Pez F,Coste I,Saintigny P,Viari A,Lang K,Guey B,Hervieu V,Bancel B,Bartoch B,Durantel D,Renno T,Merle P,Lebecque S

    更新日期：2019-10-01 00:00:00

  • Acute liver failure: A curable disease by 2024?

    abstract：:Over the last three decades acute liver failure (ALF) has been transformed from a rare and poorly understood condition with a near universally fatal outcome, to one with a well characterized phenotype and disease course. Complex critical care protocols are now applied and emergency liver transplantation (ELT) is an es...

    journal_title：Journal of hepatology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jhep.2014.12.016

    authors： Bernal W,Lee WM,Wendon J,Larsen FS,Williams R

    更新日期：2015-04-01 00:00:00

  • Modified PAGE-B score predicts the risk of hepatocellular carcinoma in Asians with chronic hepatitis B on antiviral therapy.

    abstract：BACKGROUND & AIMS:Recently, the PAGE-B score and Toronto HCC risk index (THRI) have been developed to predict the risk of hepatocellular carcinoma (HCC) in Caucasian patients with chronic hepatitis B (CHB). We aimed to validate PAGE-B scores and THRI in Asian patients with CHB and suggested modified PAGE-B scores to im...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2018.07.018

    authors： Kim JH,Kim YD,Lee M,Jun BG,Kim TS,Suk KT,Kang SH,Kim MY,Cheon GJ,Kim DJ,Baik SK,Choi DH

    更新日期：2018-11-01 00:00:00

  • Virology analyses of HCV isolates from genotype 1-infected patients treated with simeprevir plus peginterferon/ribavirin in Phase IIb/III studies.

    abstract：BACKGROUND & AIMS:Simeprevir is an oral hepatitis C virus (HCV) NS3/4A protease inhibitor approved for treatment of chronic HCV infection. Baseline NS3 polymorphisms in all patients and emerging mutations in patients who failed to achieve sustained virologic response (SVR) with simeprevir plus peginterferon/ribavirin (...

    journal_title：Journal of hepatology

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.jhep.2014.11.032

    authors： Lenz O,Verbinnen T,Fevery B,Tambuyzer L,Vijgen L,Peeters M,Buelens A,Ceulemans H,Beumont M,Picchio G,De Meyer S

    更新日期：2015-05-01 00:00:00

  • The hepatitis B virus and the host response.

    abstract：:The clearance of hepatitis B virus infected cells from the liver is probably dependent on an interplay between the interferon system and the cellular limb of the host immune response. Although the importance of the nucleocapsid proteins as targets for sensitized cytotoxic T cells is established in chronic hepatitis B ...

    journal_title：Journal of hepatology

    pub_type： 杂志文章,评审

    doi：10.1016/0168-8278(90)90170-v

    authors： Thomas HC

    更新日期：1990-01-01 00:00:00

  • The effect of antioxidant supplementation on a serum marker of free radical activity and abnormal serum biochemistry in alcoholic patients admitted for detoxification.

    abstract：:Alcoholics admitted for detoxification were entered into a double blind placebo controlled trial of oral supplementation with an antioxidant cocktail (vitamin E, beta carotene, vitamin C and selenium) in order to determine the effect of this supplementation on the rate of resolution of a serum marker of free radical a...

    journal_title：Journal of hepatology

    pub_type： 临床试验,杂志文章

    doi：10.1016/s0168-8278(05)80183-7

    authors： Butcher GP,Rhodes JM,Walker R,Krasner N,Jackson MJ

    更新日期：1993-08-01 00:00:00

  • Hepatitis D virus replication is sensed by MDA5 and induces IFN-β/λ responses in hepatocytes.

    abstract：BACKGROUND & AIMS:Hepatitis B virus (HBV) and D virus (HDV) co-infections cause the most severe form of viral hepatitis. HDV induces an innate immune response, but it is unknown how the host cell senses HDV and if this defense affects HDV replication. We aim to characterize interferon (IFN) activation by HDV, identify ...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2018.02.021

    authors： Zhang Z,Filzmayer C,Ni Y,Sültmann H,Mutz P,Hiet MS,Vondran FWR,Bartenschlager R,Urban S

    更新日期：2018-07-01 00:00:00