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Hepatic immunohistochemical staining with a monoclonal antibody against HCV-E2 to evaluate antiviral therapy and reinfection of liver grafts in hepatitis C viral infection.

Abstract：

BACKGROUND/AIMS:A simple and reproducible hepatic immunohistochemical staining (IHS) for hepatitis C virus (HCV) is not available. We aimed to validate hepatic IHS with monoclonal antibody (Mab) IG222, directed against the HCV-envelope 2 (E2) protein. METHODS:A three-step indirect immunoperoxidase method was used for frozen sections and a two-step indirect EnVision technique was used for paraffin-embedded sections. RESULTS:Naturally or in vitro HCV infected primary human hepatocytes were immunoreactive to HCV-E2. In the patient study (n=253), IHS had a sensitivity of 96% and a specificity of 91%. Six patients who showed positivity in the liver with Mab IG222, but remained anti-HCV and HCV-RNA negative, had hepatitis C-like changes in their liver biopsy. In one patient HCV-RNA could be detected in the liver biopsy. We confirmed early graft reinfection in patients transplanted for HCV-related disease (34 patients with serial biopsies). Treatment for acute cellular rejection with steroids was associated with an increase in staining intensity. In nine patients with clearance of HCV-RNA during antiviral therapy, seven achieved negativation of immunoreactivity and two a marked reduction. CONCLUSIONS:The IHS with Mab IG222 is an accurate tool for diagnosis and clinical management of chronic hepatitis C.

journal_name

J Hepatol

journal_title

Journal of hepatology

authors

Verslype C,Nevens F,Sinelli N,Clarysse C,Pirenne J,Depla E,Maertens G,van Pelt J,Desmet V,Fevery J,Roskams T

doi

10.1016/s0168-8278(02)00389-6

subject

Has Abstract

pub_date

2003-02-01 00:00:00

pages

208-14

issue

2

eissn

0168-8278

issn

1600-0641

pii

S0168827802003896

journal_volume

38

pub_type

杂志文章

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