Abstract：
BACKGROUND/AIMS:Cholangiocarcinoma comprises 5-20% of all primary malignant tumors of the liver. However, the lack of information about the genetic basis of cholangiocarcinoma has impeded characterization and understanding of its biological behavior. METHODS:In this study, genome-wide aberrations in 13 cases of cholangiocarcinoma were examined by the molecular cytogenetic technique, comparative genomic hybridization. RESULTS:Frequent gains of 1q, 3q, 8q, 15q and 17q, and common losses on 3p, 4q, 6q, 9p, 17p and 18q were found. The finding of common chromosome 3p loss (approximately 40%) with a minimal deleted region of 3p13-p21 has prompted our further investigation on the tumor suppressor gene RASSF1A, located at 3p21.3. Using bisulphite modification followed by methyl-specific PCR, a high incidence of methylated RASSF1A promoter region was detected in our current series (9/13 cases; 69%). Further expression analysis on the nine cases with promoter hypermethylation indicated much reduced RASSF1A expression compared to normal livers. CONCLUSIONS:Our current molecular cytogenetic investigation on primary cholangiocarcinoma provided overall karyotypic information and represents the first report on the methylation status of RASSF1A in cholangiocarcinoma. The high incidence of 3p loss and RASSF1A promoter hypermethylation detected may have implications for this tumor suppressor gene in the malignant progression of cholangiocarcinoma.
journal_name
J Hepatoljournal_title
Journal of hepatologyauthors
Wong N,Li L,Tsang K,Lai PB,To KF,Johnson PJdoi
10.1016/s0168-8278(02)00269-6subject
Has Abstractpub_date
2002-11-01 00:00:00pages
633-9issue
5eissn
0168-8278issn
1600-0641pii
S0168827802002696journal_volume
37pub_type
杂志文章abstract：:The liver contributes to immune surveillance against pathogens entering via the gut and is itself influenced by alterations in mucosal immune responses and the microbiome. Mucosal immunity is also implicated in autoimmune liver diseases that associate with inflammatory bowel disease (IBD), and in steatohepatitis where...
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abstract：:The prevalence of antibodies to hepatitis C virus (anti-HCV) in a hepatitis B virus hyperendemic region was evaluated with a second-generation enzyme immunoassay. A general population of 1500 from Northern Taiwan was tested with this immunoassay which includes synthetic peptides from the capsid and non-structural prot...
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journal_title：Journal of hepatology
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journal_title：Journal of hepatology
pub_type： 杂志文章,meta分析,评审
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更新日期：2011-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：1991-07-01 00:00:00
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pub_type： 杂志文章
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更新日期：1992-03-01 00:00:00
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pub_type： 杂志文章
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更新日期：1998-03-01 00:00:00
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pub_type： 杂志文章,随机对照试验
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journal_title：Journal of hepatology
pub_type： 杂志文章
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journal_title：Journal of hepatology
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更新日期：2014-06-01 00:00:00
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journal_title：Journal of hepatology
pub_type： 临床试验,杂志文章
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abstract：BACKGROUND/AIMS:Oxidative DNA damage, identifiable in the formation of 8-hydroxydeoxyguanosine (8-OHdG), is relevant in the mutagenesis/carcinogenesis process. The aim of this study was to assess 8-OHdG levels in patients with hepatitis C virus (HCV) infection in relation to extent of liver damage and HCV genotype. ME...
journal_title：Journal of hepatology
pub_type： 杂志文章
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更新日期：2001-04-01 00:00:00
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journal_title：Journal of hepatology
pub_type： 杂志文章
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更新日期：2018-04-01 00:00:00
abstract：BACKGROUND/AIMS:The hepatocyte receptor for asialoglycoproteins internalizes galactosyl terminating macromolecules which can be used as hepatotropic drug carriers. Since this receptor is also expressed on the cells of well differentiated human hepatocellular carcinomas (HCCs), we studied whether conjugation of doxorubi...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2005.02.045
更新日期：2005-10-01 00:00:00
abstract：:The hepatitis B virus (HBV) is a small enveloped DNA virus, which primarily infects hepatocytes and causes acute and persistent liver disease. Epidemiological studies have provided overwhelming evidence for a causal role of chronic HBV infection in the development of hepatocellular carcinoma, but the molecular mechani...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
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更新日期：2010-04-01 00:00:00
abstract：BACKGROUND/AIMS:The ability to transfer foreign genes into the biliary tract would be useful for the treatment of biliary tract diseases, including cancer, cystic fibrosis and other genetic diseases. To introduce a foreign gene precisely into the rat biliary epithelial cells, we developed a new technique, inserting a p...
journal_title：Journal of hepatology
pub_type： 杂志文章
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更新日期：1999-05-01 00:00:00
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journal_title：Journal of hepatology
pub_type： 杂志文章,评审
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更新日期：2019-02-01 00:00:00
abstract：:Hepatitis viruses are major threats to human health. During the last decade, highly diverse viruses related to human hepatitis viruses were found in animals other than primates. Herein, we describe both surprising conservation and striking differences of the unique biological properties and infection patterns of human...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：10.1016/j.jhep.2018.11.010
更新日期：2019-03-01 00:00:00
abstract：:Dermatological adverse events (AEs) are an existing concern during hepatitis C virus (HCV) infection and peginterferon/ribavirin treatment. HCV infection leads to dermatological and muco-cutaneous manifestations including small-vessel vasculitis as part of the mixed cryoglobulinemic syndrome. Peginterferon/ribavirin t...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：10.1016/j.jhep.2011.08.006
更新日期：2012-02-01 00:00:00
