当前位置： SCI文献检索 > JOURNAL OF HEPATOLOGY期刊下所有文献 > Loss of Sept4 exacerbates liver fibrosis through the dysregulation of hepatic stellate cells.

Loss of Sept4 exacerbates liver fibrosis through the dysregulation of hepatic stellate cells.

Abstract：

BACKGROUND/AIMS:Septins are ubiquitous and multifunctional scaffold proteins involved in cytoskeletal organization, exocytosis and other cellular processes. We disclose the quiescent hepatic stellate cells (HSCs)-specific expression of a septin subunit Sept4 in the liver, and explore the significance of the septin system in liver fibrosis. METHODS:We analyzed the expression of alpha-smooth muscle actin (alpha-SMA), collagens and other markers in primary cultured HSCs derived from wild-type and Sept4(-/-) mice. We compared susceptibility of these mice to liver fibrosis induced by either carbon tetrachloride treatment, bile duct ligation or methionine/choline-deficient diet. Collagen deposition, the principal parameter of liver fibrosis, was quantified both histochemically (Masson's trichrome stain) and biochemically (hydroxyproline content). RESULTS:In vitro, Sept4 mRNA/protein was remarkably downregulated in HSCs through myofibroblastic transformation. Sept4(-/-) HSCs showed normal morphology and proliferation, while myofibroblastic transformation as monitored by the upregulation of alpha-SMA and collagen was accelerated compared to wild-type HSCs. In vivo, liver fibrosis was consistently more severe in Sept4(-/-) mice than in wild-type littermates in all of the three paradigms of hepatitis/liver fibrosis. CONCLUSIONS:These data concordantly indicate that the HSC-specific septin subunit Sept4 and perhaps the septin system are involved in the suppressive modulation of myofibroblastic transformation and fibrogenesis associated with liver diseases.

journal_name

J Hepatol

journal_title

Journal of hepatology

authors

Iwaisako K,Hatano E,Taura K,Nakajima A,Tada M,Seo S,Tamaki N,Sato F,Ikai I,Uemoto S,Kinoshita M

doi

10.1016/j.jhep.2008.05.026

subject

Has Abstract

pub_date

2008-11-01 00:00:00

pages

768-78

issue

5

eissn

0168-8278

issn

1600-0641

pii

S0168-8278(08)00360-7

journal_volume

49

pub_type

杂志文章

相关文献

JOURNAL OF HEPATOLOGY文献大全
  • Effects of silymarin in alcoholic patients with cirrhosis of the liver: results of a controlled, double-blind, randomized and multicenter trial.

    abstract：BACKGROUND/AIMS:Silymarin has protective effects in different experimental conditions, but its efficacy in human liver cirrhosis has not been completely established. Therefore, this study was carried out to determine the effect of silymarin in alcoholics with liver cirrhosis with respect to survival and clinical and la...

    journal_title：Journal of hepatology

    pub_type： 临床试验,杂志文章,多中心研究,随机对照试验

    doi：10.1016/s0168-8278(98)80285-7

    authors： Parés A,Planas R,Torres M,Caballería J,Viver JM,Acero D,Panés J,Rigau J,Santos J,Rodés J

    更新日期：1998-04-01 00:00:00

  • Immunization against hepatitis C virus with a fusion protein containing the extra domain A from fibronectin and the hepatitis C virus NS3 protein.

    abstract：BACKGROUND/AIMS:Vaccination strategies able to induce strong T-cell responses might contribute to eradicate hepatitis C virus (HCV) infection. We previously demonstrated that fusion of an antigen to the extra domain A from fibronectin (EDA) targets the antigen to TLR4-expressing dendritic cells (DC) and improves its im...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2009.06.005

    authors： Mansilla C,Gorraiz M,Martinez M,Casares N,Arribillaga L,Rudilla F,Echeverria I,Riezu-Boj JI,Sarobe P,Borrás-Cuesta F,Prieto J,Lasarte JJ

    更新日期：2009-09-01 00:00:00

  • Expression of MAGE, GAGE and BAGE genes in human liver diseases: utility as molecular markers for hepatocellular carcinoma.

    abstract：BACKGROUND/AIMS:The MAGE, GAGE and BAGE genes encode tumor antigens recognized by autologous cytotoxic T lymphocytes. The aim of this study was to evaluate the possibility of using these genes as molecular markers and as the targets of specific immunotherapy for human hepatocellular carcinoma (HCC). METHODS:The expres...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/s0168-8278(00)80223-8

    authors： Kobayashi Y,Higashi T,Nouso K,Nakatsukasa H,Ishizaki M,Kaneyoshi T,Toshikuni N,Kariyama K,Nakayama E,Tsuji T

    更新日期：2000-04-01 00:00:00

  • Decrease in serum ALT and increase in serum HCV RNA during pregnancy in women with chronic hepatitis C.

    abstract：BACKGROUND/AIMS:The natural history of chronic hepatitis C infection during pregnancy has not been clearly established, and thus our aim was to assess serum alanine aminotransferase levels and serum HCV RNA levels during pregnancy. METHODS:Twenty-six pregnant women with chronic hepatitis C were studied. Serum alanine ...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/s0168-8278(00)80075-6

    authors： Gervais A,Bacq Y,Bernuau J,Martinot M,Auperin A,Boyer N,Kilani A,Erlinger S,Valla D,Marcellin P

    更新日期：2000-02-01 00:00:00

  • Histological and virological features and follow-up of hepatitis C virus carriers with normal aminotransferase levels: the Italian prospective study of the asymptomatic C carriers (ISACC).

    abstract：BACKGROUND/AIMS:To evaluate demographic characteristics, liver histology and virological features of hepatitis C virus (HCV) carriers with normal alanine transaminase (ALT) levels. METHODS:A nationwide prospective study was started in 1997. Four Italian centres have participated in this study. RESULTS:Eight hundred a...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/s0168-8278(02)00101-0

    authors： Puoti C,Castellacci R,Montagnese F,Zaltron S,Stornaiuolo G,Bergami N,Bellis L,Precone DF,Corvisieri P,Puoti M,Minola E,Gaeta GB

    更新日期：2002-07-01 00:00:00

  • Mitochondrial abnormalities in non-alcoholic steatohepatitis.

    abstract：BACKGROUND/AIMS:We assessed mitochondrial morphology by electron microscopy and the prevalence of a mitochondrial gene deletion in patients with non-alcoholic steatohepatitis (NASH), alcohol-related liver disease and non-fatty liver diseases. Respiratory chain function using a cytoplasmic hybrid (cybrid) assay was furt...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/s0168-8278(99)80033-6

    authors： Caldwell SH,Swerdlow RH,Khan EM,Iezzoni JC,Hespenheide EE,Parks JK,Parker WD Jr

    更新日期：1999-09-01 00:00:00

  • Gene transfer to the rat biliary tract with the HVJ-cationic liposome method.

    abstract：BACKGROUND/AIMS:The ability to transfer foreign genes into the biliary tract would be useful for the treatment of biliary tract diseases, including cancer, cystic fibrosis and other genetic diseases. To introduce a foreign gene precisely into the rat biliary epithelial cells, we developed a new technique, inserting a p...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/s0168-8278(99)80137-8

    authors： Uehara T,Honda K,Hatano E,Terao R,Iimuro Y,Yamamoto N,Yamamoto M,Kaneda Y,Yamaoka Y

    更新日期：1999-05-01 00:00:00

  • Fibrosing cholestatic hepatitis in a renal transplant recipient associated with the hepatitis B virus precore mutant.

    abstract：:A patient with evidence of chronic infection with hepatitis B virus (seropositive for hepatitis B surface antigen, and with antibody to hepatitis B e antigen) underwent renal transplantation, which subsequently failed. The patient developed abnormal liver function tests and 8 months after the removal of the transplant...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/0168-8278(95)80116-2

    authors： Booth JC,Goldin RD,Brown JL,Karayiannis P,Thomas HC

    更新日期：1995-04-01 00:00:00

  • Evaluation of a bioartificial liver based on a nonwoven fabric bioreactor with porcine hepatocytes in pigs.

    abstract：BACKGROUND/AIMS:We developed a bioartificial liver (BAL) based on a direct hemoperfusion typed nonwoven fabric bioreactor containing porcine hepatocytes. In this study, the efficacy of our BAL was evaluated with a pig fulminant hepatic failure (FHF) model. METHODS:FHF was induced with intravenous administration of D-g...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2005.08.006

    authors： Li LJ,Du WB,Zhang YM,Li J,Pan XP,Chen JJ,Cao HC,Chen Y,Chen YM

    更新日期：2006-02-01 00:00:00

  • Effects of ursodeoxycholate, its glucuronide and disulfate and beta-muricholate on biliary bicarbonate concentration and biliary lipid excretion.

    abstract：:We previously reported that high-dose ursodeoxycholate (UDC) infusion in rats resulted in extensive glucuronidation of UDC, and speculated that the glucuronidation causes bicarbonate-rich hypercholeresis induced by UDC (Takikawa, H., Sano, N., Narita, T. and Yamanaka, M. Hepatology 1990; 11: 743-749). To test this hyp...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/0168-8278(92)90015-h

    authors： Takikawa H,Sano N,Minagawa K,Yamanaka M

    更新日期：1992-05-01 00:00:00

  • Treatment of chronic hepatitis B with interferon alpha-2b and interleukin-2.

    abstract：:A total of 37 patients with histologically confirmed chronic viral hepatitis B and presence of HBV-DNA and HBsAg in the serum were treated in a randomized, prospectively controlled multicenter trial either with recombinant IFN alpha-2b alone or a combination of IFN alpha-2b and recombinant IL-2. Twenty-two patients fr...

    journal_title：Journal of hepatology

    pub_type： 临床试验,杂志文章,多中心研究,随机对照试验

    doi：10.1016/s0168-8278(05)80424-6

    authors： Bruch HR,Korn A,Klein H,Markus R,Malmus K,Baumgarten R,Müller R

    更新日期：1993-01-01 00:00:00

  • Early post-transplant survival: Interaction of MELD score and hospitalization status.

    abstract：BACKGROUND & AIMS:Urgency-based allocation that relies on the MELD score prioritizes patients at the highest risk of waitlist mortality. However, identifying patients at greatest risk for short-term post-transplant mortality is needed in order to optimize the potential gains in overall survival obtained through improve...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2015.03.034

    authors： Bittermann T,Makar G,Goldberg DS

    更新日期：2015-09-01 00:00:00

  • Evaluation and comparison of different hepatitis C virus genotyping and serotyping assays.

    abstract：BACKGROUND/AIMS:Evidence that the geno/subtype of hepatitis C virus (HCV) is predictive of the response to interferon-alpha therapy suggests that typing methods are clinically useful. In the present study, HCV isolates obtained from 74 patients with chronic hepatitis C were used to evaluate three genotyping and two ser...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/s0168-8278(97)80108-0

    authors： Lee JH,Roth WK,Zeuzem S

    更新日期：1997-05-01 00:00:00

  • Demonstration of cooperative contribution of MET- and EGFR-mediated STAT3 phosphorylation to liver regeneration by exogenous suppressor of cytokine signalings.

    abstract：BACKGROUND/AIMS:As conditional knockout mice for stat3 are impaired in liver regeneration after partial hepatectomy while those for gp130 have defects in early STAT3 phosphorylation but have normal DNA synthesis, late STAT3 phosphorylation induced independently of gp130 seems to be essential for liver regeneration. Sin...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2007.08.020

    authors： Seki E,Kondo Y,Iimuro Y,Naka T,Son G,Kishimoto T,Fujimoto J,Tsutsui H,Nakanishi K

    更新日期：2008-02-01 00:00:00

  • Assessment of hepatitis B virus DNA levels in chronic HBsAg carriers with or without hepatitis delta virus superinfection.

    abstract：:In order to document the incidence of hepatitis delta virus (HDV) replication markers and their relationship to HBV replication, 91 HBsAg chronic carriers were studied. Of these, 51 were anti-HD-positive (19 HBeAg-positive and 32 anti-HBe-positive). Liver HDAg was found in 75% of anti-HD-positive patients. Of the 19 p...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/s0168-8278(88)80033-3

    authors： Bas C,Bartolomé J,La Banda F,Porres JC,Quiroga JA,Mora I,Carreño V

    更新日期：1988-04-01 00:00:00

  • Long-term native liver fibrosis in biliary atresia: development of a novel scoring system using histology and standard liver tests.

    abstract：BACKGROUND & AIMS:Although liver fibrosis is an important predictor of outcomes for biliary atresia (BA), postsurgical native liver histology has not been well reported. Here, we retrospectively evaluated postsurgical native liver histology, and developed and assessed a novel scoring system - the BA liver fibrosis (BAL...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2014.01.028

    authors： Tomita H,Masugi Y,Hoshino K,Fuchimoto Y,Fujino A,Shimojima N,Ebinuma H,Saito H,Sakamoto M,Kuroda T

    更新日期：2014-06-01 00:00:00

  • IL-6 pathway in the liver: From physiopathology to therapy.

    abstract：:Interleukin 6 (IL-6) is a pleiotropic four-helix-bundle cytokine that exerts multiple functions in the body. In the liver, IL-6 is an important inducer of the acute phase response and infection defense. IL-6 is furthermore crucial for hepatocyte homeostasis and is a potent hepatocyte mitogen. It is not only implicated...

    journal_title：Journal of hepatology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jhep.2016.02.004

    authors： Schmidt-Arras D,Rose-John S

    更新日期：2016-06-01 00:00:00

  • Hepatic regeneration in insulin-like growth factor binding protein-1 transgenic mice.

    abstract：BACKGROUND/AIMS:Partial hepatectomy results in activation of genes in the residual liver tissue which serve to restore glucose homeostasis and regenerate liver mass. Expression of insulin-like growth factor binding protein-1 (IGFBP-1) is up-regulated following partial hepatectomy and IGFBP-1 can modulate both the metab...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/s0168-8278(99)80199-8

    authors： Liu YY,Rajkumar K,Murphy LJ

    更新日期：1999-04-01 00:00:00

  • Differences and similarities in early atherosclerosis between patients with non-alcoholic steatohepatitis and chronic hepatitis B and C.

    abstract：BACKGROUND/AIMS:To compare carotid intima-media thickness (IMT) - an index of early atherosclerosis - among patients with non-alcoholic steatohepatitis (NASH), patients with chronic hepatitis B (HBV) or C (HCV) and control subjects. METHODS:We studied 60 consecutive patients with biopsy-proven NASH, 60 patients with H...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2007.01.021

    authors： Targher G,Bertolini L,Padovani R,Rodella S,Arcaro G,Day C

    更新日期：2007-06-01 00:00:00

  • Intrahepatic IL-8 producing Foxp3⁺CD4⁺ regulatory T cells and fibrogenesis in chronic hepatitis C.

    abstract：BACKGROUND & AIMS:Regulatory CD4(+) T cells (Tregs) are considered to affect outcomes of HCV infection, because they increase in number during chronic hepatitis C and can suppress T-cell functions. METHODS:Using microarray analysis, in situ immunofluorescence, ELISA, and flowcytometry, we characterised functional diff...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2013.04.011

    authors： Langhans B,Krämer B,Louis M,Nischalke HD,Hüneburg R,Staratschek-Jox A,Odenthal M,Manekeller S,Schepke M,Kalff J,Fischer HP,Schultze JL,Spengler U

    更新日期：2013-08-01 00:00:00

  • Experimental cholestatic liver disease through bile-duct ligation in rats results in skeletal fragility and impaired osteoblastogenesis.

    abstract：BACKGROUND/AIMS:Patients with cholestatic liver disease have 'low-turnover' osteoporosis. Since we reported that bile-duct ligated (BDL) rats develop bone disease with low bone formation and mass, we examined whether their reduced bone mass results in skeletal fragility, and whether the reduction in osteoprogenitor cel...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2003.11.032

    authors： Weinreb M,Pollak RD,Ackerman Z

    更新日期：2004-03-01 00:00:00

  • Altered pathogenicity in the liver induced by a mouse hepatitis virus type 3 thermosensitive mutant.

    abstract：:Intraperitoneal inoculation into sensitive BALB/c mice of D85, a thermosensitive (ts) mutant, provokes acute hepatitis followed by recovery of the mice. The ts mutant was able to replicate in the liver. However, the maximal viral titre was obtained 2 days later than was the case with the wild-type (wt) MHV 3 infection...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/0168-8278(91)90865-9

    authors： Martin JP,Bingen A,Koehren F,Gut JP,Kirn A

    更新日期：1991-07-01 00:00:00

  • An ultrasound scoring system for the diagnosis of liver disease in cystic fibrosis.

    abstract：:Advances in the management of the pulmonary complications of cystic fibrosis may result in an increasing prevalence of patients with chronic liver disease which may, therefore, become more important in the long-term management of cystic fibrosis patients. However, no simple and reliable test is available for the diagn...

    journal_title：Journal of hepatology

    pub_type： 临床试验,杂志文章

    doi：10.1016/0168-8278(95)80444-7

    authors： Williams SG,Evanson JE,Barrett N,Hodson ME,Boultbee JE,Westaby D

    更新日期：1995-05-01 00:00:00

  • Matrix metalloproteinase-9 contributes to brain extravasation and edema in fulminant hepatic failure mice.

    abstract：BACKGROUND/AIMS:Fulminant hepatic failure (FHF) can be dreadful. When coma sets in, brain edema develops taking FHF into a lethal course. Mechanisms of brain extravasation leading to brain edema remain incompletely understood. Matrix metalloproteinase (MMP)-9 is implicated in various brain injuries. We hypothesized tha...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2005.09.019

    authors： Nguyen JH,Yamamoto S,Steers J,Sevlever D,Lin W,Shimojima N,Castanedes-Casey M,Genco P,Golde T,Richelson E,Dickson D,McKinney M,Eckman CB

    更新日期：2006-06-01 00:00:00

  • Ethanol binging exacerbates sinusoidal endothelial and parenchymal injury elicited by acetaminophen.

    abstract：BACKGROUND/AIMS:The pathophysiology of binge drinking of ethanol and its potentiation of acetaminophen (APAP) toxicity has received very little attention. To evaluate if ethanol binging sensitizes hepatic sinusoidal endothelial cells (SEC) and liver to APAP toxicity. METHODS:The histopathological responses to APAP wer...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2004.11.033

    authors： McCuskey RS,Bethea NW,Wong J,McCuskey MK,Abril ER,Wang X,Ito Y,DeLeve LD

    更新日期：2005-03-01 00:00:00

  • Inhibition of nitric oxide production increases dimethylnitrosamine-induced liver injury in rats.

    abstract：:Intravascular coagulation is involved in the development of certain types of liver injury, including that induced by dimethylnitrosamine. Nitric oxide inhibits platelet aggregation and adhesion; however, its role in protecting against intravascular coagulation has not been clarified. We therefore investigated the effe...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/0168-8278(95)80068-9

    authors： Nagase S,Isobe H,Ayukawa K,Sakai H,Nawata H

    更新日期：1995-11-01 00:00:00

  • The lessons from in vitro lithotripsy for the clinical treatment of gallstones.

    abstract：:Fifty-eight gallbladder stones were treated in vitro, using a Wolf Piezolith 2300 lithotripter, to discover whether complete fragmentation (i.e., no particles greater than 2 mm diameter) could be achieved by lithotripsy alone. In 16 stones this was accomplished with up to 6000 shocks being administered. The degree of ...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/0168-8278(89)90082-2

    authors： Bird NC,Frost EA,Kanaris-Sotiriou R,Johnson AG

    更新日期：1989-07-01 00:00:00

  • Management of anemia induced by triple therapy in patients with chronic hepatitis C: challenges, opportunities and recommendations.

    abstract：:The addition of protease inhibitors, boceprevir or telaprevir, to peginterferon+ribavirin (PegIFN/RBV) increases the frequency as well as the severity, and hence, clinical relevance of anemia, which has now become one of the major complications associated with triple therapy. Most significant factors associated with a...

    journal_title：Journal of hepatology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jhep.2013.07.014

    authors： Romero-Gómez M,Berenguer M,Molina E,Calleja JL

    更新日期：2013-12-01 00:00:00

  • Induction of chronic cholangitis in the rat by trinitrobenzenesulfonic acid.

    abstract：:We investigated the effects of the hapten trinitrobenzenesulfonic acid instilled into the rat biliary tree. The study included three groups of animals that received a single intracholedochal injection of either saline, 10% ethanol or 10 mg trinitrobenzenesulfonic acid dissolved in 10% ethanol. A fourth group of rats w...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/0168-8278(95)80432-3

    authors： Mourelle M,Salas A,Vilaseca J,Guarner F,Malagelada JR

    更新日期：1995-02-01 00:00:00

  • Human hepatocyte cell lines proliferating as cohesive spheroid colonies in alginate markedly upregulate both synthetic and detoxificatory liver function.

    abstract：BACKGROUND/AIMS:Bio-artificial liver support systems for treatment of hepatic failure require maintained expression of hepatocyte function in vitro. We studied cultures of human hepatocyte cell-lines proliferating within alginate beads, investigating the hypothesis that 3-dimensional cohesive colonies of hepatocyte cel...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/s0168-8278(00)00080-5

    authors： Khalil M,Shariat-Panahi A,Tootle R,Ryder T,McCloskey P,Roberts E,Hodgson H,Selden C

    更新日期：2001-01-01 00:00:00