Multicenter evaluation of the MB-Redox medium compared with radiometric BACTEC system, mycobacteria growth indicator tube (MGIT), and Löwenstein-Jensen medium for detection and recovery of acid-fast bacilli.

abstract：:Respiratory syncytial virus (RSV) causes serious respiratory tract infection worldwide. The relatively low RSV load makes it difficult to detect in frail, elderly, and severely immune-compromised patients. In the present study, we developed a locked nucleic acid--based 1-tube nested real-time RT-PCR (OTNRT-PCR) assay ...

journal_title：Diagnostic microbiology and infectious disease

authors： Zhao L,Wang J,Li GX,Qiu FZ,Chen C,Zhao MC,Wang L,Duan SX,Feng ZS,Ma XJ

更新日期：2019-02-01 00:00:00

abstract：:The correlation between in vitro antimicrobial activity and the in vivo response to antimicrobial therapy is affected by multiple host factors, the site and nature of the infection, and the pharmacokinetics of the antimicrobial and its penetration into areas of infection. In certain instances, discrepancies are also c...

journal_title：Diagnostic microbiology and infectious disease

authors： Washington JA 2nd

更新日期：1983-03-01 00:00:00

abstract：:This study defines the characteristics of decreasing vancomycin susceptibility in multiple isolates of methicillin-resistant Staphylococcus aureus (MRSA) recovered from a hospitalized patient in Canada over a period of 6 months. The MICs of the isolates increased during therapy with vancomycin. The patient fractured h...

journal_title：Diagnostic microbiology and infectious disease

authors： Webster D,Rennie RP,Brosnikoff CL,Chui L,Brown C

更新日期：2007-02-01 00:00:00

abstract：:Interferon gamma release assays are used for diagnosing latent tuberculosis (TB); however, their role in diagnosing tuberculous pleural effusion (TPE) is not defined. The aim of this study was to evaluate the usefulness of the QuantiFERON-TB Gold In-Tube assay (QFT-IT) and compare this assay with the tuberculin skin t...

journal_title：Diagnostic microbiology and infectious disease

authors： Chung JH,Han CH,Kim CJ,Lee SM

更新日期：2011-11-01 00:00:00

abstract：:The mechanism and clinical relevance of the inhibitory effect of ciprofloxacin on the metabolism of selected drugs were studied in patients with bacterial infections. In study A, antipyrine tests were carried out in two groups of patients taking 1000 mg (group 1) and 250 mg (group 2) of oral ciprofloxacin for 7-10 day...

journal_title：Diagnostic microbiology and infectious disease

authors： Ludwig E,Graber H,Székely E,Csiba A

更新日期：1990-03-01 00:00:00

abstract：:The influence of the pH of urine on the detection of cytomegalovirus (CMV) by the shell vial assay was evaluated. The pH values of 295 urine specimens ranged from 4.7 to 8.5 (mean 6.3) and were not significantly different in culture-positive versus culture-negative samples. None of the urine specimens appeared to be t...

journal_title：Diagnostic microbiology and infectious disease

authors： Gray LD,Ilstrup DM,Smith TF

更新日期：1989-05-01 00:00:00

abstract：:In a collection of 50 pvl-positive Staphylococcus aureus isolates from 10 Rio de Janeiro hospitals, 18 (36%) were from bloodstream infections, and 31 (62%) carried the SCCmec IV. Among 25 (50%) isolates of the USA1100/ST30/CC30 lineage present in 8 hospitals, 1 isolate was characterized as vancomycin-intermediate S. a...

journal_title：Diagnostic microbiology and infectious disease

authors： Chamon RC,Iorio NL,da Silva Ribeiro S,Cavalcante FS,Dos Santos KR

更新日期：2015-12-01 00:00:00

abstract：:In 2003, a 10-month-old Ghanaian boy recovered from a Trypanosoma brucei infection, although the patient was not treated with antitrypanosomal drugs. Only T. brucei gambiense and T. brucei rhodesiense are able to infect humans, causing human African trypanosomiasis. The disease is considered 100% fatal if left untreat...

journal_title：Diagnostic microbiology and infectious disease

authors： Deborggraeve S,Koffi M,Jamonneau V,Bonsu FA,Queyson R,Simarro PP,Herdewijn P,Büscher P

更新日期：2008-08-01 00:00:00

abstract：:Development of the ARCHITECT Toxo IgM assay has been done to assist the clinician in acute Toxoplasma gondii infection detection, especially in pregnant women. Its use, in conjunction with ARCHITECT Toxo IgG and Toxo Avidity assays, will provide an array of assays particularly useful in the monitoring of pregnant fema...

journal_title：Diagnostic microbiology and infectious disease

authors： Sickinger E,Braun HB,Praast G,Stieler M,Gundlach C,Birkenbach C,Prostko J,Palafox MA,Frias E,Hsu S,Matias M,Pucci D,Hausmann M,Sagel U,Smith D

更新日期：2009-07-01 00:00:00

abstract：:Detection of specific IgM antibodies by ELISA forms the mainstay for diagnosis of dengue infection. However, IgM antibodies develop after 4 to 5 days of infection. The methods for early diagnosis include virus isolation and reverse transcriptase polymerase chain reaction (RT-PCR) which need a sophisticated laboratory....

journal_title：Diagnostic microbiology and infectious disease

authors： Singh MP,Majumdar M,Singh G,Goyal K,Preet K,Sarwal A,Mishra B,Ratho RK

更新日期：2010-09-01 00:00:00

abstract：:Methods currently used for susceptibility testing of Mycobacterium, including agar dilution and the Bactec radiometric method, are based on the proportion technique using a single critical concentration of antibiotic. Preliminary studies were conducted with Mycobacterium species by using the Etest method, a gradient m...

journal_title：Diagnostic microbiology and infectious disease

authors： Wanger A,Mills K

更新日期：1994-07-01 00:00:00

abstract：:Biomarkers such as procalcitonin (PCT) have been studied to guide duration of antibiotic therapy. We aimed to assess whether a decrease in PCT levels could be used to reduce the duration of antibiotic therapy in intensive care unit (ICU) patients with a proven infection without risking a worse outcome. We assessed 265...

journal_title：Diagnostic microbiology and infectious disease

authors： Deliberato RO,Marra AR,Sanches PR,Martino MD,Ferreira CE,Pasternak J,Paes AT,Pinto LM,dos Santos OF,Edmond MB

更新日期：2013-07-01 00:00:00

abstract：:An in vitro study of the activity of 9 agents against 181 US pediatric isolates of Streptococcus pneumoniae identified imipenem and faropenem as the most active agents. Overall, faropenem was the most potent oral agent inhibiting 98% of isolates at 1 microg/mL. ...

journal_title：Diagnostic microbiology and infectious disease

authors： Black JA,Moland ES,Chartrand SA,Thomson KS

更新日期：2001-09-01 00:00:00

abstract：:We assessed the performance of the ARCHITECT Toxo IgG, IgM, and IgG Avidity assays against corresponding assays on AxSYM and Vidas using 730 sera from pregnant women. The ARCHITECT Toxo IgG and IgM assays showed a relative sensitivity of 97.5% and 89.9% and a relative specificity of 99.1% and 99.8%, respectively. If I...

journal_title：Diagnostic microbiology and infectious disease

authors： Gay-Andrieu F,Fricker-Hidalgo H,Sickinger E,Espern A,Brenier-Pinchart MP,Braun HB,Pelloux H

更新日期：2009-11-01 00:00:00

abstract：:This prospective study evaluated the ability of the qPCR Amplidiag® CarbaR+VRE assay to detect Carbapenemase-producing Gram-negative bacilli (CP-GNB) directly on 1830 rectal swabs extracted using the fully automated platform Amplidiag® Easy instrument. The Amplidiag® CarbaR+VRE assay gave a positive signal for 94 rect...

journal_title：Diagnostic microbiology and infectious disease

authors： Nadji S,Gaillot O,Hallaert C,Messaadi S,Loukili N,Naas T,Lemaître N

更新日期：2019-12-01 00:00:00

abstract：:Deoxyribonucleic acid sequences specific for a pathogen of interest can be isolated from a variety of microorganisms. Such DNA probes can be exploited to detect infectious agents directly in infected patient material despite the presence of large numbers of other organisms and host DNA. This technology is dependent up...

journal_title：Diagnostic microbiology and infectious disease

authors： Horn JE,Quinn T,Hammer M,Palmer L,Falkow S

更新日期：1986-03-01 00:00:00

abstract：:We conducted a retrospective review of the clinical data on patients with polymicrobial candidemia during a nine-year period (1992-2000) at our tertiary care University Hospital. Also, the clinical features of patients with polymicrobial vs monomicrobial candidemia were compared. There were 16 (5.2%) patients with mul...

journal_title：Diagnostic microbiology and infectious disease

authors： Pulimood S,Ganesan L,Alangaden G,Chandrasekar P

更新日期：2002-12-01 00:00:00

abstract：:Purulent pericarditis is a cardiac emergency that can be difficult to diagnose and can be rapidly fatal. We report the case of a child with Down syndrome and recent atrial and ventricular septal defect repair who died from Haemophilus influenzae serotype f pericarditis. ...

journal_title：Diagnostic microbiology and infectious disease

authors： Taube JM,Hutchins GM,Carroll KC,Vricella LA,Scheel J,Halushka MK

更新日期：2006-09-01 00:00:00

abstract：:During March 2005, 24 cases of acute human Chagas disease were detected in Santa Catarina State, southern Brazil, all of them related to the ingestion of Trypanosoma cruzi-contaminated sugar cane juice. Following field studies allowed the isolation of 13 T. cruzi strains from humans, opossums (Didelphis aurita and Did...

journal_title：Diagnostic microbiology and infectious disease

authors： Steindel M,Kramer Pacheco L,Scholl D,Soares M,de Moraes MH,Eger I,Kosmann C,Sincero TC,Stoco PH,Murta SM,de Carvalho-Pinto CJ,Grisard EC

更新日期：2008-01-01 00:00:00

abstract：:The prevalence of low-level resistance to glycopeptides (teicoplanin MIC > or = 8 microg/mL and vancomycin MIC > or = 4 microg/mL) among staphylococci was investigated over a 15 month period. A total of 2,279 isolates (1,519 S. aureus, 760 coagulase-negative staphylococcus (CNS)) were screened using inoculum of 10(6) ...

journal_title：Diagnostic microbiology and infectious disease

authors： Park YJ,Jun Park J,Lee SO,Oh EJ,Kee Kim B

更新日期：2001-11-01 00:00:00

abstract：:Clinical non-relevant (CNR) IgM specific for Toxoplasma gondii is responsible for false-positive results in commercially available IgM assays. Using IgM immunoblotting, it is possible to distinguish between IgM in sera of acutely infected (AI) patients and CNR IgM. Especially the combination of staining of a 55 and 30...

journal_title：Diagnostic microbiology and infectious disease

authors： Meek B,Diepersloot RJ,van Gool T,Speijer D,Peek R

更新日期：2003-01-01 00:00:00

abstract：:Utilization of rapid microbiologic methods could improve both the quality of patient care and help to reduce patient health care costs through more efficient patient management. Medically urgent situations such as meningitis, endocarditis, bacteremia, some soft tissue infections, ocular infections, and pneumonia in im...

journal_title：Diagnostic microbiology and infectious disease

authors： Jorgensen JH

更新日期：1985-11-01 00:00:00

abstract：:Haemophilus influenzae, a frequent colonizer of the respiratory tract, is the causative agent of several clinically important infections. In cases that require therapeutic intervention, laboratory susceptibility testing can detect beta-lactam antibiotic resistance and guide the best treatment course. In the absence of...

journal_title：Diagnostic microbiology and infectious disease

authors： Schotte L,Wautier M,Martiny D,Piérard D,Depypere M

更新日期：2019-03-01 00:00:00

abstract：:The study evaluated the change in the clinical antifungal sensitivity profile of A. flavus strains after exposure to azole and benzimidazole fungicide. Exposure to fungicide altered the sensitivity profile for the antifungal itraconazole, voriconazole and posaconazole. This change was characterized by an increase in t...

journal_title：Diagnostic microbiology and infectious disease

authors： Meireles LM,de Araujo ML,Endringer DC,Fronza M,Scherer R

更新日期：2019-10-01 00:00:00

abstract：:We analyzed sequences of graSR, vraSR, walKR and rpoB genes in hVISA from Brazil. Five isolates showed mutations in at least one gene. rpoB H481N and graS T224I were the most frequent mutations, followed by graR D148Q and walK A468T. Our study reinforces the heterogeneity of genetic patterns among hVISA. ...

journal_title：Diagnostic microbiology and infectious disease

authors： Silveira ACO,Caierão J,Silva CI,Anzai EK,McCulloch JA,d'Azevedo PA,Sincero TCM

更新日期：2019-11-01 00:00:00

abstract：:Borrelia burgdorferi sensu lato (s. l.), the agent of Lyme disease, is distributed widely worldwide. A large number of polymerase chain reaction (PCR) methods have been developed and used for detection of B. burgdorferi s. l. However, there is a lack of a reference standard because of the genetic diversity of the B. b...

journal_title：Diagnostic microbiology and infectious disease

authors： Yang J,Liu Z,Guan G,Che R,Niu Q,Li Y,Liu J,Ma M,Ren Q,Liu A,Luo J,Yin H

更新日期：2012-05-01 00:00:00

abstract：:The impact of accessory gene regulator (agr) dysfunction and high bacterial density on vancomycin killing and resistance was evaluated among 10 clinical methicillin-resistant Staphylococcus aureus bloodstream isolates using time kill experiments. Under conditions of high inocula and agr dysfunction, vancomycin activit...

journal_title：Diagnostic microbiology and infectious disease

authors： Tsuji BT,Harigaya Y,Lesse AJ,Sakoulas G,Mylotte JM

更新日期：2009-06-01 00:00:00

abstract：:Despite improvement in vaccinations, Hepatitis B remains a major health concern due to the difficulty of prevention even in low endemic areas such as Europe. In this report we describe the performance characteristics of the new HBsAg Next Qualitative and HBsAg Next Confirmatory assays designed for blood screening and ...

journal_title：Diagnostic microbiology and infectious disease

authors： Sickinger E,Braun HB,Meyer T,Schmid K,Daghfal D,Oer M,Schultess J

更新日期：2020-06-01 00:00:00

abstract：:A patient presented with inflamed hands and Erysipelothrix rhusiopathiae bacteremia. Because a high incidence of endocarditis has been reported with this organism, a transesophageal echocardiogram was obtained, which was normal. Treatment with oral moxifloxacin resolved all manifestations of illness. The association b...

journal_title：Diagnostic microbiology and infectious disease

authors： Drekonja DM

更新日期：2013-11-01 00:00:00

abstract：:We developed and evaluated a method to genotypically identify enterotoxigenic Escherichia coli (ETEC) and to characterize these organisms with respect to 18 of 21 known colonization factors (CFs). The method, which is based on polynucleotide DNA-DNA colony hybridization, includes a pooled toxin probe assay to identify...

journal_title：Diagnostic microbiology and infectious disease

authors： Steinsland H,Valentiner-Branth P,Grewal HM,Gaastra W,Mølbak K K,Sommerfelt H

更新日期：2003-02-01 00:00:00