Exposure of resting peripheral blood T cells to HIV-1 particles generates CD25+ killer cells in a small subset, leading to induction of apoptosis in bystander cells.


:Apoptosis is a major mechanism whereby HIV-1 depletes uninfected CD4+ and CD8+ T cells. We previously showed that resting peripheral blood T cells derived from healthy donors were killed by an apoptotic mechanism after adsorption to gp120-containing, protease-defective HIV-1 (L-2) particles, more effectively than parental wild-type LAI adsorption or rgp 120-mediated CD4 cross-linking, followed by mitogenic stimulation. Here, we present evidence that the L-2 particle-based apoptosis was induced both in CD4+ and CD8+ cells by generation of effector cells which were mainly derived from a resting memory CD4+CD38- subset. This subset enhanced the CD25 expression on the surface and secreted IFN-gamma in the culture supernatant after L-2 particle exposure. Significant elevation of Fas ligand mRNA was found in the subset by L-2 particle exposure, while expression of Fas antigen on uninfected T cells was induced by exposure to IFN-gamma. These results indicate that L-2 particles can shift the CD4+CD38- subpopulation from a resting to an activated state, and this activation leads to killing of bystander CD4+ and CD8+ T cells by a Fas-mediated mechanism. In fact, purified CD4+CD38- cells exposed to L-2 particles were converted into effector cells that were able to kill autologous as well as allogenic target T cells pretreated with IFN-gamma. Further, we found that the observation of apoptosis due to L-2 particles was a more general phenomenon, that also occurred with Thai primary HIV-1 isolates. These results suggest that such specific types of HIV-1 particles may play a major role in the induction of apoptosis for both bystander CD4+ and CD8+ T cells, through inappropriate activation of CD4+CD38- cells.


Int Immunol


International immunology


Kameoka M,Suzuki S,Kimura T,Fujinaga K,Auwanit W,Luftig RB,Ikuta K




Has Abstract


1997-10-01 00:00:00












  • Characterization of the developmental landscape of murine RORγt+ iNKT cells.

    abstract::Invariant natural killer T (iNKT) cells expressing the retinoic acid receptor-related orphan receptor γt (RORγt) and producing IL-17 represent a minor subset of CD1d-restricted iNKT cells (iNKT17) in C57BL/6J (B6) mice. We aimed in this study to define the reasons for their low distribution and the sequence of events ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Klibi J,Li S,Amable L,Joseph C,Brunet S,Delord M,Parietti V,Jaubert J,Marie J,Karray S,Eberl G,Lucas B,Toubert A,Benlagha K

    更新日期:2020-02-07 00:00:00

  • DNA vaccination with gp96-peptide fusion proteins induces protection against an intracellular bacterial pathogen.

    abstract::Effective vaccination using in vitro peptide-loaded heat-shock proteins (HSP), tumor-derived HSP and HSP fusion proteins has been shown in viral, parasite and tumor model systems. We demonstrate protective DNA vaccination using gp96-peptide fusion proteins against the intracellular bacterial pathogen Listeria monocyto...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Rapp UK,Kaufmann SH

    更新日期:2004-04-01 00:00:00

  • CD1d-dependent expansion of NKT follicular helper cells in vivo and in vitro is a product of cellular proliferation and differentiation.

    abstract::NKT follicular helper cells (NKTfh cells) are a recently discovered functional subset of CD1d-restricted NKT cells. Given the potential for NKTfh cells to promote specific antibody responses and germinal center reactions, there is much interest in determining the conditions under which NKTfh cells proliferate and/or d...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Rampuria P,Lang ML

    更新日期:2015-05-01 00:00:00

  • Immunomodulation by hepatitis C virus-derived proteins: targeting human dendritic cells by multiple mechanisms.

    abstract::Hepatitis C virus (HCV) has the ability to persist in the majority of infected people. Strong, multispecific and sustained T-cell response is correlated with viral clearance. The mechanisms of chronicity by HCV are unclear. HCV could restrain the immune system and establish chronic infection by modulating dendritic ce...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Krishnadas DK,Ahn JS,Han J,Kumar R,Agrawal B

    更新日期:2010-06-01 00:00:00

  • Anti-CD3 induces bi-phasic apoptosis in murine intestinal epithelial cells: possible involvement of the Fas/Fas ligand system in different T cell compartments.

    abstract::Recent studies have suggested that Fas-mediated apoptosis is involved in the pathogenesis of intestinal injury. In this study, we determined the role of Fas/Fas ligand (FasL) interactions in different T cell compartments using a murine model of small intestinal injury. An intraperitoneal injection of 145-2C11 (anti-CD...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Miura N,Yamamoto M,Fukutake M,Ohtake N,Iizuka S,Ishige A,Sasaki H,Fukuda K,Yamamoto T,Hayakawa S

    更新日期:2005-05-01 00:00:00

  • Normal thymic selection, superantigen-induced deletion and Fas-mediated apoptosis of T cells in IL-2 receptor beta chain-deficient mice.

    abstract::Mice lacking the IL-2 receptor beta chain (IL-2R beta) exhibit an autoimmune reaction characterized by generalized T cell activation, production of autoantibodies, myeloproliferation and severe anemia. T cells of IL-2R beta-/- mice were examined to elucidate the mechanism responsible for their abnormal activation and ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Suzuki H,Hayakawa A,Bouchard D,Nakashima I,Mak TW

    更新日期:1997-09-01 00:00:00

  • Modulation of CD4 co-receptor limits spontaneous autoimmunity when high-affinity transgenic TCR specific for self-antigen is expressed on a genetically resistant background.

    abstract::Myelin proteolipid protein (PLP) 139-151 is an immunodominant peptide that induces experimental autoimmune encephalomyelitis (EAE) in H-2(s) SJL/J mice. While PLP 139-151-specific TCR transgenic (tg) 4E3 mice develop fulminant spontaneous disease on the susceptible SJL/J background, spontaneous EAE is dramatically red...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Illés Z,Waldner H,Reddy J,Anderson AC,Sobel RA,Kuchroo VK

    更新日期:2007-10-01 00:00:00

  • PD-1 and PD-1 ligands: from discovery to clinical application.

    abstract::Programmed cell death-1 (PD-1, Pdcd1), an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. Because of the wide range of ligand distribution in the body, its b...

    journal_title:International immunology

    pub_type: 杂志文章,评审


    authors: Okazaki T,Honjo T

    更新日期:2007-07-01 00:00:00

  • Peripheral blood CD4+CD8+ lymphocytes in cynomolgus monkeys are of resting memory T lineage.

    abstract::In this study, we analyzed peripheral blood CD4+CD8+ double-positive (DP) lymphocytes in adult cynomolgus monkeys (Macaca fascicularis). Forty of 55 monkeys had > 5% of the peripheral blood DP subpopulation (9.3 +/- 5.9%; mean +/- SD) in peripheral blood lymphocytes (PBL) in contrast to a low percentage of peripheral ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Akari H,Terao K,Murayama Y,Nam KH,Yoshikawa Y

    更新日期:1997-04-01 00:00:00

  • Synergistic T-T cell interaction present in alloreactivity: determination of 'MLR helper' T cell subsets.

    abstract::CD4 and CD8 T cell subsets involved in primary allo-MLR responses were re-evaluated in the present study. The slope analysis of B6 allo-MLR responses to MHC class I-disparate bm1 and class II-disparate bm12 revealed the presence of a T-T cell interaction between CD4 and CD8 T cell subsets. The allo-MLR response of B6 ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Asano Y,Komuro T,Tada T

    更新日期:1990-01-01 00:00:00

  • Autoreactive human T cell lines recognizing ribosomal protein L7.

    abstract::Sera of patients suffering from systemic lupus erythematosus (SLE) frequently contain oligoclonal IgG autoantibodies with high affinity for the ribosomal protein L7 (rpL7). The humoral autoimmune response to rpL7 apparently is driven by antigen and T cell dependent. In order to analyze the T cell response to rpL7 we c...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Donauer J,Wochner M,Witte E,Peter HH,Schlesier M,Krawinkel U

    更新日期:1999-02-01 00:00:00

  • Induction of ornithine decarboxylase activity in mouse B lymphocytes.

    abstract::We have examined the role in B cell activation of ornithine decarboxylase (ODC), the labile rate-limiting enzyme in the synthesis of polyamines thought to be required for S phase entry in all cells. When small resting mouse splenic B cells were stimulated with the mitogenic agents phorbol myristate acetate (PMA) plus ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Molitor J,Pace W,Stunz L,Vigna J,Louie S,Ashman R

    更新日期:1994-11-01 00:00:00

  • Lipid mediators and sterile inflammation in ischemic stroke.

    abstract::Stroke is one of the major causes of lethality and disability, yet few effective therapies have been established for ischemic stroke. Inflammation in the ischemic brain is induced by the infiltration and subsequent activation of immune cells. Loss of cerebral blood flow and ischemic brain-cell death trigger the activa...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Nakamura A,Otani K,Shichita T

    更新日期:2020-10-20 00:00:00

  • Recombinant nucleocapsid-like particles from dengue-2 virus induce protective CD4+ and CD8+ cells against viral encephalitis in mice.

    abstract::Virus-like particles are a highly effective type of subunit vaccine that mimics the overall structure of virus particles without containing infectious genetic material. In this work, a particulate form of the recombinant capsid protein from dengue-2 was evaluated in mice to determine the level of protection against vi...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Gil L,López C,Lazo L,Valdés I,Marcos E,Alonso R,Gambe A,Martín J,Romero Y,Guzmán MG,Guillén G,Hermida L

    更新日期:2009-10-01 00:00:00

  • Role of the CD5 molecule on TCR gammadelta T cell-mediated immune functions: development of germinal centers and chronic intestinal inflammation.

    abstract::Although CD4(+) T cells form a major subset of TCRalphabeta T cells, only a small number of TCRgammadelta T cells express CD4. Factors contributing to the down-regulation of CD4(+) TCRgammadelta T cells have not been identified. The CD5 molecule is expressed on most TCRgammadelta T cells in the spleen, whereas only a ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Mizoguchi A,Mizoguchi E,de Jong YP,Takedatsu H,Preffer FI,Terhorst C,Bhan AK

    更新日期:2003-01-01 00:00:00

  • Induction of CD8+ cytotoxic T lymphocytes by immunization with syngeneic irradiated HIV-1 envelope derived peptide-pulsed dendritic cells.

    abstract::Based on the evidence that CD8+ cytotoxic T cells (CTL) precursors do not appear to distinguish between virus-infected cells and viral peptide-pulsed syngeneic cells, we have developed methods for priming class I MHC molecule restricted CD8+ CTL with such peptides without using any adjuvant. We were able to prime in v...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Takahashi H,Nakagawa Y,Yokomuro K,Berzofsky JA

    更新日期:1993-08-01 00:00:00

  • MHC class II-mediated apoptosis of mature dendritic cells proceeds by activation of the protein kinase C-delta isoenzyme.

    abstract::The mature dendritic cell (DC) is considered to be the most potent antigen-presenting cell. Regulation of the DC, particularly its survival, is therefore critical. Mature DC are markedly more sensitive to HLA-DR-mediated apoptosis than immature DC. To further characterize this key survival difference, we compared the ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Bertho N,Blancheteau VM,Setterblad N,Laupeze B,Lord JM,Drénou B,Amiot L,Charron DJ,Fauchet R,Mooney N

    更新日期:2002-08-01 00:00:00

  • Fas deficiency in mice with the Balb/c background induces blepharitis with allergic inflammation and hyper-IgE production in conjunction with severe autoimmune disease.

    abstract::Fas (CD95) is a cell surface death receptor belonging to the tumor necrosis factor receptor superfamily, which mediates apoptosis-inducing signaling when activated by Fas ligand or its agonistic antibody. lpr mice with a loss of apoptosis-inducing function mutation in the Fas gene develop systemic autoimmune disease a...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Takahashi S,Futatsugi-Yumikura S,Fukuoka A,Yoshimoto T,Nakanishi K,Yonehara S

    更新日期:2013-05-01 00:00:00

  • Negative autoregulation of Src homology region 2-domain-containing phosphatase-1 in rat basophilic leukemia-2H3 cells.

    abstract::Src homology region 2-domain-containing phosphatase-1 (SHP-1) plays an important role in the regulation of signaling from various receptors in hematopoietic cells. In mast cells, SHP-1 has been shown to negatively regulate the initial signaling triggered by high-affinity receptor for IgE (FcepsilonRI) and positively r...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Ozawa T,Nakata K,Mizuno K,Yakura H

    更新日期:2007-09-01 00:00:00

  • Homing of lymphocytes into islets of Langerhans in prediabetic non-obese diabetic mice is not restricted to autoreactive T cells.

    abstract::Non-obese diabetic mice spontaneously develop a type 1 diabetes. The entry of leukocytes in the islets of Langerhans was studied in untreated and in irradiated mice. FITC-labeled cells from spleen, lymph nodes or bone marrow of healthy or diabetic donors did home to the inflamed islets of unmanipulated recipients. B a...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Faveeuw C,Gagnerault MC,Kraal G,Lepault F

    更新日期:1995-12-01 00:00:00

  • Normal D-JH rearranged products of the IgH gene in SCID mouse bone marrow.

    abstract::SCID mice are profoundly immunodeficient, resulting from an inability to carry out the V(D)J recombination reaction during both B cell and T cell development. Recently, however, it was revealed that normal rearrangement frequently did occur in the TCR delta and gamma chain loci in the SCID thymus. To evaluate whether ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Araki R,Itoh M,Hamatani K,Abe M

    更新日期:1996-07-01 00:00:00

  • Activation-induced expression of murine CD83 on T cells and identification of a specific CD83 ligand on murine B cells.

    abstract::Human CD83 is a cell surface protein expressed predominantly by dendritic cells (DC) and lymphoid cells. So far, there exists no information on the function and distribution of mCD83. Here we demonstrate that mCD83 is moderately expressed on resting T cells and DC, but strongly increases in its expression on T cells f...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Cramer SO,Trumpfheller C,Mehlhoop U,Moré S,Fleischer B,von Bonin A

    更新日期:2000-09-01 00:00:00

  • PolyI:C-induced reduction in uptake of soluble antigen is independent of dendritic cell activation.

    abstract::Dendritic cells (DC) are key players in the initiation and modulation of adaptive immune responses due to their ability to acquire and present antigen and stimulate T cells. For the induction of effector T cell functions, antigen must be presented by activated DC. In this study, we have compared uptake of antigen by m...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Tirapu I,Giquel B,Alexopoulou L,Uematsu S,Flavell R,Akira S,Diebold SS

    更新日期:2009-07-01 00:00:00

  • A DNase I hypersensitive site near the murine gamma1 switch region contributes to insertion site independence of transgenes and modulates the amount of transcripts induced by CD40 ligation.

    abstract::Several cis-acting elements regulate the expression of germline transcripts of heavy chain constant region genes and their subsequent switch recombination. To study such elements in the murine gamma1 gene, we have utilized a transgenic approach. In this study we focused on a DNase I hypersensitive site (termed 'Site I...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Adams K,Ackerly H,Cunningham K,Dunnick W

    更新日期:2000-12-01 00:00:00

  • HIV envelope-directed signaling aberrancies and cell death of CD4+ T cells in the absence of TCR co-stimulation.

    abstract::HIV-1 infection in CD4(+) T cells initiates a viral cytopathic effect (CPE) that is dependent on the activation of intracellular protein tyrosine kinases (PTK). PTK in T cells are also activated during the course of TCR or CD4 receptor engagement and the manner of receptor engagement may generate signals leading eithe...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Tian H,Lempicki R,King L,Donoghue E,Samelson LE,Cohen DI

    更新日期:1996-01-01 00:00:00

  • Differential role of MyD88 signaling in Streptococcus suis serotype 2-induced systemic and central nervous system diseases.

    abstract::Streptococcus suis serotype 2 is an important porcine bacterial pathogen and a zoonotic agent responsible for sudden death, septic shock and meningitis, with exacerbated inflammation being a hallmark of the systemic and central nervous system (CNS) infections. However, S. suis serotype 2 strains are genetically and ph...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Auger JP,Benoit-Biancamano MO,Bédard C,Segura M,Gottschalk M

    更新日期:2019-10-16 00:00:00

  • Selective recognition of malaria antigens by human serum antibodies is not genetically determined but demonstrates some features of clonal imprinting.

    abstract::Malaria infection induces the production of serum antibodies to a variety of malaria antigens but the prevalence of antibodies to any particular antigen is typically much less than 100%. It has been assumed that non-responsiveness to defined antigens in malaria immune subjects is due to HLS-mediated restriction of the...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Taylor RR,Egan A,McGuinness D,Jepson A,Adair R,Drakely C,Riley E

    更新日期:1996-06-01 00:00:00

  • Production of citrulline and ornithine by interferon-gamma treated macrophages.

    abstract::Activated macrophages exert strong arginase (ASE) activity that converts L-arginine into ornithine, the key precursor for putrescine and polyamine biosynthesis. Macrophages were previously also shown to generate nitric oxide that is derived from the guanido group of arginine by the oxidative deiminase (OAD) reaction. ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Benninghoff B,Lehmann V,Eck HP,Dröge W

    更新日期:1991-05-01 00:00:00

  • Multiple nuclear factors bind to novel positive and negative regulatory elements upstream of the human MHC class I gene HLA-A11.

    abstract::Quantitative expression of class I genes varies widely in different tissues, during development and in some neoplastic cells. Regulatory DNA sequences controlling levels of transcription of class I genes have been characterized in the murine and swine promoters. Although some regulatory features appear to be retained ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Blanchet O,Gazin C,L'Haridon M,Tatari Z,Degos L,Sigaux F,Paul P

    更新日期:1994-10-01 00:00:00

  • MHC class II and invariant chain biosynthesis and transport during maturation of human precursor dendritic cells.

    abstract::Dendritic cells (DC) are highly potent activators of the immune response. The precise mechanisms that give rise to the DC phenotype are not known. To investigate the mechanisms that contribute to the generation of the DC phenotype, precursor DC were freshly isolated from human blood and allowed to mature in vitro. The...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Engering AJ,Richters CD,Fluitsma DM,van Pelt AM,Kamperdijk EW,Hoefsmit EC,Pieters J

    更新日期:1998-11-01 00:00:00