Abstract:
:This study evaluated the attachment, chemoattractive, proliferative and mineralization inductive potential of a bovine cementum extract (CPE) on newborn murine dental follicle cells (MDFC) in vitro. Cementum extract was partially purified by DEAE-cellulose chromatography. A band representing an M(r) of 55,000 was excised from the gel and the protein(s) were electroeluted. Attachment assays revealed that CPE (1.0 microgram/ml) promoted MDFC attachment by 96% in comparison with collagen type I (5 micrograms/ml), and was five-fold greater compared with serum-free media (SFM), (P < 0.05). Between 1 and 5 days CPE at 1.0 microgram/ml and collagen type I at 5 micrograms/ml sustained more than 75% attachment and spreading of MDFC when compared to SFM (P < 0.05). Contrary to other reports, fibronectin (0.5 microgram/ml) was more potent than CPE in promoting MDFC chemoattraction (P < 0.05). MDFC proliferation was stimulated by CPE (0.125 microgram/ml), but this response was elicited only when CPE was used together with 10% FBS (37.3%) or 0.2% FBS (76%) (P < 0.05). Alkaline phosphatase expression by MDFC was increased by CPE (1.0 microgram/ml), in comparison to the control. Calcium deposits were detected by von Kossa staining in 14-day MDFC cultures treated with CPE. Nodule formation and its mineralization in long-term MDFC cultures were induced by CPE (1.0 microgram/ml). Molecule(s) contained in CPE appear to regulate various biological activities in MDFC, indicating that CPE could play a key role in selecting progenitor cells required for the process of cementogenesis during development.
journal_name
Arch Med Resjournal_title
Archives of medical researchauthors
Arzate H,Aguilar-Mendoza ME,Esponda Aguilar C,Portilla Robertson Jsubject
Has Abstractpub_date
1997-10-01 00:00:00pages
407-13issue
3eissn
0188-4409issn
1873-5487journal_volume
28pub_type
杂志文章abstract:BACKGROUND AND AIMS:Extended-spectrum β-lactamase (ESBLs) production is still the most frequent mechanism of resistance to cephalosporins in gram-negative bacteria. The aim of the study was to identify the types of ESBL-producing Enterobacteriaceae clinical isolates causing nosocomial infections in Mexico. METHODS:ESB...
journal_title:Archives of medical research
pub_type: 杂志文章,多中心研究
doi:10.1016/j.arcmed.2011.02.004
更新日期:2011-02-01 00:00:00
abstract:BACKGROUND:The aim of this study was to determine concentrations of tumor necrosis factor-alpha (TNF-alpha) in normal, painful, and asymptomatic human dental pulps. METHODS:Pulps were obtained from three groups of teeth, including healthy teeth, asymptomatic teeth with caries and/or large restoration, and symptomatic ...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/s0188-4409(02)00396-x
更新日期:2002-09-01 00:00:00
abstract:BACKGROUND:The paper analyzed the impact of war, the most pernicious of social evils, taking into consideration intensity of change in rate of suicide in the southwestern region of Croatia during the war and including three distinctly specific intervals, two peacetime-one pre-war (1986-1990) and one post-war (1996-2000...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2004.03.001
更新日期:2004-07-01 00:00:00
abstract:BACKGROUND:It is well documented that Giardia duodenalis undergoes surface antigenic variation both in vivo and in vitro. Proteins involved have been characterized and referred to as VSP (variable surface protein). METHODS:Two cloned cDNA inserts of 0.45 and 1.95 kb were obtained from G. duodenalis expression library ...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2003.12.005
更新日期:2004-05-01 00:00:00
abstract:BACKGROUND:Gamma-irradiation of platelet concentrates may inactive contaminated lymphocytes and subsequently inhibit the synthesis of cytokine in platelet during storage. The aim of this study was to determine the effect of gamma-irradiation on the production of IL-8 and lymphocyte proliferation isolated from random do...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2008.05.004
更新日期:2008-08-01 00:00:00
abstract::Global warming has serious implications for all aspects of human life, including infectious diseases. The effect of global warming depends on the complex interaction between the human host population and the causative infectious agent. From the human standpoint, changes in the environment may trigger human migration, ...
journal_title:Archives of medical research
pub_type: 杂志文章,评审
doi:10.1016/j.arcmed.2005.03.041
更新日期:2005-11-01 00:00:00
abstract:BACKGROUND AND AIMS:Despite its beneficial effects, cisplatin has considerable nephrotoxic, ototoxic, neurotoxic and hepatotoxic side effects. It has been documented that reactive oxygen radical species are involved with the pathophysiology of cisplatin-induced hepatotoxicity. Molsidomine (MOL) can exert antioxidant an...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2013.09.013
更新日期:2013-10-01 00:00:00
abstract::During the process of energy production in aerobic respiration, vascular cells produce reactive oxygen species (ROS). A growing body of evidence indicates that oxidative stress refers to a condition in which cells are subjected to excessive levels of ROS. Overall vascular function is dependent upon a fine balance of o...
journal_title:Archives of medical research
pub_type: 杂志文章,评审
doi:10.1016/j.arcmed.2005.11.012
更新日期:2006-05-01 00:00:00
abstract:BACKGROUND AND AIMS:In previous studies, the Pro12Ala polymorphism in the peroxisome proliferator-activated receptor gamma 2 (PPAR-γ2) was shown to be associated with both lipid metabolism and longevity. We examined whether the polymorphism continued to be associated with abnormal levels of serum lipid/lipoprotein amon...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2011.09.010
更新日期:2011-10-01 00:00:00
abstract::During the late 1940s, Alfred Jost demonstrated that mammalian sex differentiation begins in fetal testis, producing two factors necessary for the establishment of phenotypic males. Castrated embryos prior to testis differentiation led to phenotypic female differentiation. Jost proposed the existence of a testis-deter...
journal_title:Archives of medical research
pub_type: 杂志文章,评审
doi:10.1016/s0188-4409(01)00317-4
更新日期:2001-11-01 00:00:00
abstract:BACKGROUND:One of the most frequent malignancies in women worldwide is carcinoma of the uterine cervix. High-risk human papillomavirus (HPV) infection is considered the most important etiological factor of uterine cervical cancer. Our aim was to identify novel cellular genes that could potentially act as predictive mol...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2005.04.016
更新日期:2005-09-01 00:00:00
abstract:BACKGROUND:Vascular endothelial growth factor (VEGF), an important regulator of angiogenesis and vascular permeability, is involved in various steps of many malignancies. Gene polymorphisms within the gene encoding VEGF have been shown to be independently associated with an adverse outcome in various malignancies inclu...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2009.07.011
更新日期:2009-10-01 00:00:00
abstract:BACKGROUND:Thyrotoxic periodic paralysis (TPP) is characterized by episodes of neuromuscular weakness occurring in the context of hypokalemia and hyperthyroidism and has been predominantly described in Oriental populations. Whereas it is uncommon in Caucasians and Blacks, TPP does occur in individuals of Native America...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/s0188-0128(98)00014-1
更新日期:1999-01-01 00:00:00
abstract::Three exoglucanase genes have been described in Saccharomyces cerevisiae. The bulk of the exoglucanase (EXO) activity is encoded by the EXG1 gene, whose primary gene product is differentially glycosylated during its transit to the cell surface to yield three isoenzymes: EXOI, EXOII and EXOII1/2. EXOII, the major isoen...
journal_title:Archives of medical research
pub_type: 杂志文章,评审
doi:
更新日期:1993-10-01 00:00:00
abstract:BACKGROUND AND AIMS:Cervical cancer is a common neoplastic disease affecting women worldwide. Expression of human papillomavirus type 16 (HPV-16) E6/E7 genes is frequently associated with cervical cancer, representing ideal targets for diagnostic and therapeutic strategies. Aptamers are oligonucleotide ligands capable ...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2011.02.005
更新日期:2011-02-01 00:00:00
abstract:BACKGROUND AND AIMS:The pathogenesis of coronary artery ectasia (CE) is poorly understood. An underlying inflammatory process is suspected; current therapy aims to prevent thrombotic events. Presently, there is no evidence to support an anti-inflammatory approach for CE patients. We undertook this study to determine th...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2014.07.002
更新日期:2014-08-01 00:00:00
abstract:BACKGROUND:Afternoon nap sleep was studied in 32 young male medical students who take customary naps to replace loss in nocturnal sleep. METHODS:From 16 subjects, a group called dreamers was formed, and the other 16 individuals were grouped as non-dreamers. Polygraphic recordings lasting 30 min were done at a fixed ti...
journal_title:Archives of medical research
pub_type: 临床试验,杂志文章
doi:
更新日期:1998-07-01 00:00:00
abstract::Phagocytic host defense mechanisms play a pivotal role in the prevention and containment of invasive, disseminated candidiasis, aspergillosis, and other opportunistic mycoses. The specific processes involved depend upon both the species, growth phase, and surface properties of the fungal target and the type and differ...
journal_title:Archives of medical research
pub_type: 杂志文章,评审
doi:
更新日期:1993-01-01 00:00:00
abstract:OBJECT:Astragaloside IV (AS IV) has antioxidative and anti-apoptotic properties, however, its effects on juvenile mice with diabetic ketoacidosis (DKA) have not been determined. This study aims to explore the effect and mechanism of Astragaloside IV (AS-IV) on juvenile mice with DKA. METHODS:DKA model was established ...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2020.06.013
更新日期:2020-10-01 00:00:00
abstract::The expression of neurodegenerative diseases can be categorized into three main symptomatic domains: neurological, cognitive and, neuropsychiatric. This review focuses on the cognitive profile and neuropsychological assessment of Alzheimer's disease (AD). The topography and progression of brain neuropathology determin...
journal_title:Archives of medical research
pub_type: 杂志文章,评审
doi:10.1016/j.arcmed.2012.08.015
更新日期:2012-11-01 00:00:00
abstract:BACKGROUND:Many studies indicate that the production of reactive oxygen species (ROS) after renal ischemia/reperfusion (I/R) may initiate the cascade of cellular injury. It has been demonstrated that ozone oxidative preconditioning (OzoneOP) may prevent the damage induced by ROS and attenuate renal I/R injury. On the b...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2007.09.005
更新日期:2008-02-01 00:00:00
abstract:BACKGROUND:Patients with certain types of stroke need urgent anticoagulation and it is extremely important for them to achieve fast and stable anticoagulant effect and receive individualized treatment during the initiation of warfarin therapy. METHODS:We conducted a prospective study among 210 acute stroke patients wh...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2015.05.001
更新日期:2015-05-01 00:00:00
abstract:BACKGROUND:Hyperlipidemia is a risk factor for atherosclerotic events. Differences in lipid levels may exist in different races. Han is the largest group and Zhuang is the largest minority among the 56 nationalities in China. Geographically and linguistically, Zhuang can be classified into 43 ethnic subgroups, among wh...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2005.12.005
更新日期:2006-08-01 00:00:00
abstract:BACKGROUND:There is a need to assess whether human immunodeficiency virus (HIV)-infected patients are more likely than noninfected individuals to have any of the specific lipoprotein combination profiles identified as the best predictors of future cardiovascular disease in the general population. METHODS:One hundred f...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2007.06.012
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND AND AIMS:Previously, TP53 codon 72 polymorphisms have been implicated as risk factors for various cancers. Several studies have been conducted on the association of TP53 codon 72 polymorphisms with susceptibility to nasopharyngeal carcinoma (NPC) and have yielded inconclusive results. The aim of the present ...
journal_title:Archives of medical research
pub_type: 杂志文章,meta分析
doi:10.1016/j.arcmed.2009.03.006
更新日期:2009-05-01 00:00:00
abstract:BACKGROUND:The frequency of neurologic manifestations of the central nervous system (CNS) as a defining disease of acquired immunodeficiency syndrome (AIDS) (neurologic manifestation defining disease [NMDD-AIDS]) varies according to geographic region. The aim of this study was to determine its prevalence among patients...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2003.07.004
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND AND AIMS:It remains unclear whether U50488H (a selective kappa-opioid receptor agonist) produces anti-apoptotic effect during ischemia and reperfusion (I/R). Therefore, the effect of U50488H on myocardial apoptosis was investigated in the present study. METHODS:Rats were subjected to 45min coronary artery o...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2009.04.009
更新日期:2009-05-01 00:00:00
abstract:BACKGROUND AND AIMS:Thyroid hormones exert important effects on heart remodeling through mir-208. The process may have a role in myocardial changes in chronic kidney disease where thyroid abnormalities are common. In this study the effect of T4 supplementation on left ventricle (LV) remodeling in 5/6 nephrectomized rat...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2013.11.005
更新日期:2013-11-01 00:00:00
abstract::Pathogenesis of the development of sepsis is highly complex and has been the object of study for many years. The inflammatory phenomena underlying septic shock are described in this review, as well as the enzymes and genes involved in the cellular activation that precedes this condition. The most important molecular a...
journal_title:Archives of medical research
pub_type: 杂志文章,评审
doi:10.1016/j.arcmed.2004.07.006
更新日期:2004-11-01 00:00:00
abstract::In this report we show the chromosomal changes seen in a group of 303 Mexican patients with de novo Acute Myeloblastic Leukemia (AML). Two hundred forty-two patients were diagnosed and treated at two hospitals affiliated with the Instituto Mexicano del Seguro Social (IMSS). These are the Centro Medico Nacional Siglo X...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:
更新日期:1997-07-01 00:00:00