Abstract:
:To measure thyroid-stimulating antibody (TSAb) in sera from patients with Graves' disease, we developed a new assay system with using frozen stocks of CHO-K1 cells. CHO-K1 cells transfected with cloned thyrotropin (TSH) receptor on a 96 well plate were frozen in Cell Banker and stored at -70 degrees C. Three days before the assay, they were thawed in the culture medium and allowed to grow in a monolayer until use. The medium was replaced with medium containing IgGs from the patients, then after 2 h, it was collected and concentrations of adenosine 3'-5'-cyclicmonophosphate (cAMP) were measured. This method is sensitive enough to detect TSAb and it is simpler and easier than the methods which use FRTL-5 cells.
journal_name
Endocr Jjournal_title
Endocrine journalauthors
Takano T,Sumizaki H,Amino Ndoi
10.1507/endocrj.44.431subject
Has Abstractpub_date
1997-06-01 00:00:00pages
431-5issue
3eissn
0918-8959issn
1348-4540journal_volume
44pub_type
杂志文章abstract::Recent studies indicate that succinate dehydrogenase (SDH) genes B, C, or D are, at least partly, involved in the pathogenesis of pheochromocytoma or paraganglioma. Of these three genes, the SDHD gene mutation is most closely related with paragangliomas of the neck. Here we describe a case of an SDHD-related paragangl...
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journal_title:Endocrine journal
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journal_title:Endocrine journal
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doi:
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pub_type: 杂志文章
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更新日期:2015-01-01 00:00:00
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更新日期:2005-04-01 00:00:00
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更新日期:2007-12-01 00:00:00
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更新日期:2015-01-01 00:00:00
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