Biochemical study of individual zonae from human oocytes that failed to undergo fertilization in intracytoplasmic sperm injection.

abstract：:In addition to prostaglandins, inflammatory cytokines induce uterine contraction via oxytocin (OT). Placental leucine aminopeptidase (P-LAP), an oxytocinase that is identical to cystine aminopeptidase, destroys OT activity. Patients with spontaneous preterm delivery have higher concentrations of inflammatory cytokines...

journal_title：Molecular human reproduction

authors： Ikoma Y,Nomura S,Ito T,Katsumata Y,Nakata M,Iwanaga K,Okada M,Kikkawa F,Tamakoshi K,Nagasaka T,Tsujimoto M,Mizutani S

更新日期：2003-02-01 00:00:00

abstract：:G12 monoclonal antibody (mAb), one of a library of constructed mAb directed against human sperm proteins, was found by immunoperoxidase staining to label the post-acrosomal and neck regions of fixed human cauda epididymal and ejaculated spermatozoa. Epithelium and fluid of caput epididymis were strongly labelled while...

journal_title：Molecular human reproduction

authors： Lefèvre A,Martin Ruiz C,Chokomian S,Duquenne C,Finaz C

更新日期：1997-06-01 00:00:00

abstract：:Oocyte quality is one of the most important aspects of in vitro embryo development. Extensive epigenetic programming must occur during oocyte growth and maturation. A specific DNA methylation pattern of the imprinted genes must be established on differentially methylated regions (DMR). The insulin-like growth factor 2...

journal_title：Molecular human reproduction

authors： Fagundes NS,Michalczechen-Lacerda VA,Caixeta ES,Machado GM,Rodrigues FC,Melo EO,Dode MA,Franco MM

更新日期：2011-02-01 00:00:00

abstract：:Medium-chain acyl-CoA dehydrogenase (MCAD) deficiency is the most common defect in fatty acid oxidation. The disease is inherited in an autosomal recessive fashion (carrier frequency around 1 in 70) and probably affects as many as 1 in 10000 new-borns. Affected children usually present within the two first years of li...

journal_title：Molecular human reproduction

authors： Sermon K,Henderix P,Lissens W,De Vos A,Vandervorst M,Vanderfaeillie A,Vamos E,Van Steirteghem A,Liebaers I

更新日期：2000-12-01 00:00:00

abstract：:Matrix metalloproteinases (MMP), tissue inhibitors of metalloproteinases (TIMP), and MMP-TIMP interactions may contribute to the highly programmed process of embryo implantation. The loss of the delicate MMP-TIMP balance may lead to abnormal implantation. The role of TIMP-4 in mouse implantation has not been reported....

journal_title：Molecular human reproduction

authors： Zhang J,Zhao YG,Cao YJ,Sang QX,Duan EK

更新日期：2003-03-01 00:00:00

abstract：:In order to improve assisted fertilization in humans it is important to elucidate the mechanisms of control of growth and development in the early pre-embryo. Increasing evidence shows that growth factors are of importance for such control mechanisms. As platelet-derived growth factor (PDGF) has been shown to enhance ...

journal_title：Molecular human reproduction

authors： Osterlund C,Wramsby H,Pousette A

更新日期：1996-07-01 00:00:00

abstract：:The transcription factor OCT-4 is regarded as a critical factor in controlling mammalian early embryonic development because of its role in toti-/pluripotency. In human preimplantation embryos, OCT-4 studies are limited to RNA analysis of abnormally developing embryos. This study thoroughly investigated the expression...

journal_title：Molecular human reproduction

authors： Cauffman G,Van de Velde H,Liebaers I,Van Steirteghem A

更新日期：2005-03-01 00:00:00

abstract：:FSH is a pituitary gonadotropin that along with LH plays a key role in the regulation of gonadal function. The gonadotropic hormones are composed of two subunits, the common alpha subunit and the hormone-specific beta subunit, which determines the binding to specific receptors and induction of biological response. Unl...

journal_title：Molecular human reproduction

authors： Lamminen T,Jokinen P,Jiang M,Pakarinen P,Simonsen H,Huhtaniemi I

更新日期：2005-08-01 00:00:00

abstract：:Follicular development and ovulation are major processes in the reproductive system. Understanding their complexity is important to female fertility treatments and the control of reproductive processes. Wnt signaling pathway components were shown to be involved in reproduction in animal models. The secreted frizzled-r...

journal_title：Molecular human reproduction

authors： Maman E,Yung Y,Cohen B,Konopnicki S,Dal Canto M,Fadini R,Kanety H,Kedem A,Dor J,Hourvitz A

更新日期：2011-07-01 00:00:00

abstract：:The major regulators of endometrial function are oestrogen and progesterone, which act through binding their nuclear receptors and by activating transcription of their target genes. Interactions between steroid receptors and transcription proteins, e.g. c-JUN/AP-1, can modulate steroid action at the transcriptional le...

journal_title：Molecular human reproduction

authors： Salmi A,Pakarinen P,Peltola AM,Rutanen EM

更新日期：1998-12-01 00:00:00

abstract：:Development of uterine glands (adenogenesis) in mammals typically begins during the early post-natal period and involves budding of nascent glands from the luminal epithelium and extensive cell proliferation in these structures as they grow into the surrounding stroma, elongate and mature. Uterine glands are essential...

journal_title：Molecular human reproduction

authors： Cooke PS,Spencer TE,Bartol FF,Hayashi K

更新日期：2013-09-01 00:00:00

abstract：:Regulation of germ-cell-specific transcription is essential for the differentiation and other physiological processes of germ cells. Germ-cell-specific transcription factors, Sohlh2 and Figla, play key roles in gametogenesis. To elucidate whether an epigenetic mechanism is involved in the controlled expression of Sohl...

journal_title：Molecular human reproduction

authors： Pan B,Chao H,Chen B,Zhang L,Li L,Sun X,Shen W

更新日期：2011-09-01 00:00:00

abstract：:The peri-implantation period is a critical time during murine development. Although the importance of nitric oxide has been demonstrated during gestation, its role in implantation has not been fully defined. The aim of this study was to quantify (by Western blotting) two prominent nitric oxide synthase (NOS) isoforms,...

journal_title：Molecular human reproduction

authors： Purcell TL,Given R,Chwalisz K,Garfield RE

更新日期：1999-05-01 00:00:00

abstract：:We purified by fractionation on 10-40% glycerol gradients, 26S proteasomes from normal human spermatozoa. These proteasomes, which participate in the ATP-dependent degradation of ubiquitinated proteins, share a similar sedimentation coefficient to those purified from other human tissues. Fluorogenic peptide assays rev...

journal_title：Molecular human reproduction

authors： Tipler CP,Hutchon SP,Hendil K,Tanaka K,Fishel S,Mayer RJ

更新日期：1997-12-01 00:00:00

abstract：:Polar body emission is a specialized cell division throughout the animal kingdom, serving to reduce chromosome ploidy while preserving the egg cytoplasm. Critical to polar body emission are the asymmetric positioning of the meiotic spindle prior to anaphase, with one pole attached to the oocyte cortex, and the simulta...

journal_title：Molecular human reproduction

authors： Leblanc J,Zhang X,McKee D,Wang ZB,Li R,Ma C,Sun QY,Liu XJ

更新日期：2011-05-01 00:00:00

abstract：:Male and female preimplantation mammalian embryos differ not only in their chromosomal complement, but in their proteome and subsequent metabolome. This phenomenon is due to a finite period during preimplantation development when both X chromosomes are active, between embryonic genome activation and X chromosome inact...

journal_title：Molecular human reproduction

authors： Gardner DK,Larman MG,Thouas GA

更新日期：2010-08-01 00:00:00

abstract：:The spindle assembly checkpoint modulates the timing of anaphase initiation in response to the improper alignment of chromosomes at the metaphase plate. If defects are detected, a signal is transduced to halt further progression of the cell cycle until correct bipolar attachment to the spindle is achieved. The mitotic...

journal_title：Molecular human reproduction

authors： Steuerwald N,Cohen J,Herrera RJ,Sandalinas M,Brenner CA

更新日期：2001-01-01 00:00:00

abstract：:Oocytes are usually the largest cells in the body and as such offer unique opportunities for single-cell analysis. Unfortunately, these cells are also some of the rarest in the mammalian female, usually necessitating single-cell analysis. In cases of infertility in humans, determining the quality of the oocyte is ofte...

journal_title：Molecular human reproduction

authors： Brayboy LM,Wessel GM

更新日期：2016-03-01 00:00:00

abstract：:Chemokines are implicated in the implantation process. The aim of this study was to investigate mRNA expression and protein levels of chemokine receptors CXCR1, CXCR4, CCR5 and CCR2B in human endometrium throughout the menstrual cycle, during HRT and in the human blastocyst. The regulation of chemokine receptors in th...

journal_title：Molecular human reproduction

authors： Dominguez F,Galan A,Martin JJ,Remohi J,Pellicer A,Simón C

更新日期：2003-04-01 00:00:00

abstract：:Prostaglandin F2α (PGF2α) plays a critical role in the initiation and process of parturition. Since human labor has been described as an inflammatory event, we investigated the role of PGF2α in the inflammatory process using cultured human uterine smooth muscle cells (HUSMCs) isolated from term pregnant women as a mod...

journal_title：Molecular human reproduction

authors： Xu C,Liu W,You X,Leimert K,Popowycz K,Fang X,Wood SL,Slater DM,Sun Q,Gu H,Olson DM,Ni X

更新日期：2015-07-01 00:00:00

abstract：:Advanced glycation end products (AGEs) affect the follicular microenvironment. The close relationship between AGEs, proinflammatory cytokine production and activation of the unfolded protein response (UPR), which involves activating transcription factor 4 (ATF4), is crucial for regulation of various cellular functions...

journal_title：Molecular human reproduction

authors： Takahashi N,Harada M,Azhary JMK,Kunitomi C,Nose E,Terao H,Koike H,Wada-Hiraike O,Hirata T,Hirota Y,Koga K,Fujii T,Osuga Y

更新日期：2019-11-30 00:00:00

abstract：:Type I inositol 1,4,5-trisphosphate-sensitive receptors (InsP(3)R) are expressed in human oocytes and may be involved in operating the Ca(2+) release triggered by the fertilizing sperm. This study examines the contribution of type I InsP(3)R in operating Ca(2+) release in human oocytes secondary to InsP(3) itself, usi...

journal_title：Molecular human reproduction

authors： Goud PT,Goud AP,Leybaert L,Van Oostveldt P,Mikoshiba K,Diamond MP,Dhont M

更新日期：2002-10-01 00:00:00

abstract：:Charcot-Marie-Tooth (CMT) disease type 1A is an autosomal dominant peripheral neuropathy characterized by slow progressive distal muscle wasting and weakness, and decreased nerve conduction velocities. Most CMT1A cases (>98%) are caused by a duplication of a 1.5 Mb region on the short arm of chromosome 17 containing t...

journal_title：Molecular human reproduction

authors： De Vos A,Sermon K,Van de Velde H,Joris H,Vandervorst M,Lissens W,Mortier G,De Sutter P,Löfgren A,Van Broeckhoven C,Liebaers I,Van Steirteghem A

更新日期：1998-10-01 00:00:00

abstract：:The use of interphase fluorescent in situ hybridization (FISH) has shown that a large number of human embryos exhibit chromosomal abnormalities in vitro. The most common abnormality is mosaicism which is seen in up to 50% of preimplantation embryos at all stages of development. In this study, comparative genomic hybri...

journal_title：Molecular human reproduction

authors： Daphnis DD,Fragouli E,Economou K,Jerkovic S,Craft IL,Delhanty JD,Harper JC

更新日期：2008-02-01 00:00:00

abstract：STUDY HYPOTHESIS:It is possible to isolate pure populations of single potential human spermatogonial stem cells without somatic contamination for down-stream applications, for example cell culture and gene expression analysis. STUDY FINDING:We isolated pure populations of single potential human spermatogonial stem cel...

journal_title：Molecular human reproduction

authors： von Kopylow K,Schulze W,Salzbrunn A,Spiess AN

更新日期：2016-04-01 00:00:00

abstract：:Preimplantation development shifts from a maternal to embryonic programme rapidly after fertilization. Although the majority of oogenetic products are lost during the maternal to embryonic transition (MET), several do survive this interval to contribute directly to supporting preimplantation development. Embryonic gen...

journal_title：Molecular human reproduction

authors： Bell CE,Calder MD,Watson AJ

更新日期：2008-12-01 00:00:00

abstract：:Before the advent of intracytoplasmic sperm injection (ICSI) semen preparation techniques focused on the need to sustain the fertilizing potential of the spermatozoa particularly by reducing oxidative stress. However, for severely oligozoospermic patients treated by ICSI, sperm preparation protocols are used which aim...

journal_title：Molecular human reproduction

authors： Twigg J,Irvine DS,Houston P,Fulton N,Michael L,Aitken RJ

更新日期：1998-05-01 00:00:00

abstract：:Human endometrial stromal cells (ESC) can produce a variety of chemokines, especially after inflammatory stimulation. Interferon-gamma-inducible protein-10 (IP-10) is a potent chemoattractant for lymphocytes, and belongs to the family of non-ELR CXC chemokines. The expression of IP-10 in ESC after stimulation with int...

journal_title：Molecular human reproduction

authors： Kai K,Nasu K,Nakamura S,Fukuda J,Nishida M,Miyakawa I

更新日期：2002-02-01 00:00:00

abstract：:We recently identified the DPY19L2 gene as the main genetic cause of human globozoospermia. Non-genetically characterized cases of globozoospermia were associated with DNA alterations, suggesting that DPY19L2-dependent globozoospermia may be associated with poor DNA quality. However the origins of such defects have no...

journal_title：Molecular human reproduction

authors： Yassine S,Escoffier J,Martinez G,Coutton C,Karaouzène T,Zouari R,Ravanat JL,Metzler-Guillemain C,Lee HC,Fissore R,Hennebicq S,Ray PF,Arnoult C

更新日期：2015-02-01 00:00:00

abstract：:Oligozoospermia (low sperm count) is a common semen deficiency. However, to date, few genetic defects have been identified to cause this condition. Moreover, even fewer molecular genetic diagnostic tests are available for patients with oligozoospermia in the andrology clinic. Based on animal and gene expression studie...

journal_title：Molecular human reproduction

authors： Yatsenko AN,Georgiadis AP,Murthy LJ,Lamb DJ,Matzuk MM

更新日期：2013-06-01 00:00:00