Abstract：
:Plant cell suspension cultures of Rauvolfia are able to produce a high amount of arbutin by glucosylation of exogenously added hydroquinone. A four step purification procedure using anion exchange, hydrophobic interaction, hydroxyapatite-chromatography and chromatofocusing delivered in a yield of 0.5%, an approximately 390 fold enrichment of the involved glucosyltransferase. SDS-PAGE showed a M(r) for the enzyme of 52 kDa. Proteolysis of the pure enzyme with endoproteinase LysC revealed six peptide fragments with 9-23 amino acids which were sequenced. Sequence alignment of the six peptides showed high homologies to glycosyltransferases from other higher plants.
journal_name
Phytochemistryjournal_title
Phytochemistryauthors
Arend J,Warzecha H,Stöckigt Jdoi
10.1016/s0031-9422(99)00539-7subject
Has Abstractpub_date
2000-01-01 00:00:00pages
187-93issue
2eissn
0031-9422issn
1873-3700pii
S0031-9422(99)00539-7journal_volume
53pub_type
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