Conversions of deoxyradicinin to radicinin and of radicinin to 3-epi-radicinin in the phytopathogenic fungus Bipolaris coicis.

Abstract:

:Radicinin is a phytotoxic and antibiotic metabolite produced by some phytopathogenic fungi. Precursor administration and cell-free experiments with deoxyradicinin and radicinin were carried out in Bipolaris coicis H13-3. When deoxyradicinin was administered to the fungus, radicinin and 3-epi-radicinin formed. When radicinin administered, 3-epi-radicinin was formed. Their formation was confirmed by cell-free experiments. Deoxyradicinin 3-monooxygenase which catalyzes conversion of deoxyradicinin to radicinin showed the best activity at 35 °C and pH 7.0, and required NAD+ as co-enzyme. Its molecular weight was determined to be 130-184 kDa. Radicinin epimerase catalyzing the reaction of radicinin to 3-epi-radicinin was purified from a cell-free extract. Radicinin epimerase is a homodimer of a 28 kDa subunit, and its highest activity was achieved at 30-35 °C and pH 7.0-9.0.

journal_name

Phytochemistry

journal_title

Phytochemistry

authors

Suzuki M,Sakuno E,Ishihara A,Tamura J,Nakajima H

doi

10.1016/j.phytochem.2011.11.010

subject

Has Abstract

pub_date

2012-03-01 00:00:00

pages

14-20

eissn

0031-9422

issn

1873-3700

pii

S0031-9422(11)00496-1

journal_volume

75

pub_type

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