Abstract:
:Radicinin is a phytotoxic and antibiotic metabolite produced by some phytopathogenic fungi. Precursor administration and cell-free experiments with deoxyradicinin and radicinin were carried out in Bipolaris coicis H13-3. When deoxyradicinin was administered to the fungus, radicinin and 3-epi-radicinin formed. When radicinin administered, 3-epi-radicinin was formed. Their formation was confirmed by cell-free experiments. Deoxyradicinin 3-monooxygenase which catalyzes conversion of deoxyradicinin to radicinin showed the best activity at 35 °C and pH 7.0, and required NAD+ as co-enzyme. Its molecular weight was determined to be 130-184 kDa. Radicinin epimerase catalyzing the reaction of radicinin to 3-epi-radicinin was purified from a cell-free extract. Radicinin epimerase is a homodimer of a 28 kDa subunit, and its highest activity was achieved at 30-35 °C and pH 7.0-9.0.
journal_name
Phytochemistryjournal_title
Phytochemistryauthors
Suzuki M,Sakuno E,Ishihara A,Tamura J,Nakajima Hdoi
10.1016/j.phytochem.2011.11.010subject
Has Abstractpub_date
2012-03-01 00:00:00pages
14-20eissn
0031-9422issn
1873-3700pii
S0031-9422(11)00496-1journal_volume
75pub_type
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