Enzyme immunoassays for specific IgG and IgE antibodies to Pichia pastoris components in normal humans.

abstract：:We here describe an ion-exchange high-performance liquid chromatography technique with electrochemical detection for rapid quantification of glutathione, homocysteine, cysteinylglycine, and methionine. The analytical validation of the technique showed within-assay and between-assay coefficients of variation between 3....

journal_title：Journal of clinical laboratory analysis

authors： Houze P,Gamra S,Madelaine I,Bousquet B,Gourmel B

更新日期：2001-01-01 00:00:00

abstract：BACKGROUND:Contradictory results have been reported previously in the analyses of cross-reactivity among Blomia tropicalis (Blo t), Dermatophagoides pteronyssinus (Der p), and Dermatophagoides farinae (Der f). This study aims to investigate the characteristics of co-sensitization and the IgE cross-reactivity among them...

journal_title：Journal of clinical laboratory analysis

authors： Liu X,Zheng P,Zheng SG,Zhai Y,Zhao X,Chen Y,Cai C,Wu Z,Huang Z,Zou X,Liao C,Sun B

更新日期：2019-11-01 00:00:00

abstract：:The purpose of this study was to evaluate whether IL-1 beta (IL-1beta promoter and IL-1beta exon 5), IL-1 receptor antagonist (IL-1 Ra), and IL-4 (IL-4 promoter and IL-4 intron 3) gene polymorphisms act as markers of susceptibility to Kawasaki disease (KD), or of the severity of the disease. The study included 107 KD ...

journal_title：Journal of clinical laboratory analysis

authors： Wu SF,Chang JS,Wan L,Tsai CH,Tsai FJ

更新日期：2005-01-01 00:00:00

abstract：BACKGROUND:The aim of our study was to assess two tumor markers, carbohydrate antigen 724 (CA724) and neuron-specific enolase (NSE), in 20 healthy individuals. Then, we determined their average intra- and inter-individual biological variations (CVI and CVG ). METHODS:Blood samples were taken at weekly intervals for fi...

journal_title：Journal of clinical laboratory analysis

authors： Wang S,Zhao M,Mu R,Zhang X,Yun K,Shang H

更新日期：2018-05-01 00:00:00

abstract：BACKGROUND:Gastric carcinoma (GC) is a familiar carcinoma and serious threat to human health. We investigated the efficacy and mechanism of circular RNA hsa_circ_0001649 on the growth, migration, and invasion of GC cells. METHODS:microRNA (miR)-20a and hsa_circ_0001649 expression was investigated by RT-qPCR and was ch...

journal_title：Journal of clinical laboratory analysis

authors： Sun H,Wang Q,Yuan G,Quan J,Dong D,Lun Y,Sun B

更新日期：2020-06-01 00:00:00

abstract：:A radioactive polymerase chain reaction (PCR) method has been developed for the relative quantification of the human alpha-2 chain of type I collagen [hu alpha-2(I)] in cells. cDNAs generated by reverse transcription from the total pool of cytoplasmic RNA serve as a template for polymerase chain reaction amplification...

journal_title：Journal of clinical laboratory analysis

authors： Yu YL,Golden JA,Migchielsen AA,Goetzl EJ,Turck CW

更新日期：1991-01-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay (ELISA) for the detection of antitetanus antibodies was developed using aluminum-absorbed tetanus toxoid as the coating antigen. The assay was tested by measuring antitetanus antibody levels in serum obtained from subjects before and after immunization with the aluminum-absorbed te...

journal_title：Journal of clinical laboratory analysis

authors： Thiele GM,Rogers J,Collins M,Yasuda N,Smith D,McDonald TL

更新日期：1990-01-01 00:00:00

abstract：:The newly developed immunoblot assay, RIBA SIA (recombinant and synthetic polypeptide immunoblot assay), Chiron, Calif., was compared with the commercially available second generation recombinant immunoblot assay (RIBA-2) for the detection of antibody to hepatitis C virus (anti-HCV). The two immunoblot tests were also...

journal_title：Journal of clinical laboratory analysis

authors： Chaudhary RK,Andonov A,MacLean C

更新日期：1993-01-01 00:00:00

abstract：:Previous studies have shown elevated serum levels of interleukin 6 (IL-6) and C-reactive protein (CRP) in patients with pancreatic cancer (PC). The aim of this study was to assess the diagnostic usefulness of pretreatment serum levels of IL-6 and CRP to differentiate between PC and chronic pancreatitis (CP) patients. ...

journal_title：Journal of clinical laboratory analysis

authors： Mroczko B,Groblewska M,Gryko M,Kedra B,Szmitkowski M

更新日期：2010-01-01 00:00:00

abstract：BACKGROUND:Osteoarthritis (OA) is a popular arthrosis featured as pain, limited joint activity, and deformity. Cyclooxygenase-2 (COX-2) has been reported to be up-regulated in arthritic tissues and is integral to the progression of osteoarthritis (OA). Previous studies showed the COX-2 promoter G-765C polymorphism coul...

journal_title：Journal of clinical laboratory analysis

authors： Huang W,Deng C,Tian F,Gao W,Ding Z,Rao X,Yin Z

更新日期：2018-07-01 00:00:00

abstract：BACKGROUND:Essential trace elements are vital to human health. In this study, our aim was to establish reference intervals of and to evaluate relationships among Ca, Fe, Cu, Mg, and Zn for children. METHODS:We collected blood samples of 3210 children aged 0-14 years from Lu'an, China, and concentrations of the above e...

journal_title：Journal of clinical laboratory analysis

authors： Zhai R,Zhang M,Liu J,Guang H,Li B,Chen D,Zhang S

更新日期：2017-03-01 00:00:00

abstract：BACKGROUND:Enzyme-linked immunosorbent assay (ELISA) has traditionally been used to detect myeloperoxidase (MPO) and proteinase 3 (PR3) antibodies, although it is time-consuming and physically demanding. As a novel and highly effective immunoassay, we compared chemiluminescent immunoassay (CIA) with ELISA to verify the...

journal_title：Journal of clinical laboratory analysis

authors： Hou X,Liu J,Wang T,Zhou J,Cui L

更新日期：2020-10-09 00:00:00

abstract：:The effects of TPA (12-0-tetradecanoylphorbol-13-acetate) and RA (retinoic acid) were investigated on the cell lines HL60 (acute promyelocytic leukemia) and K562 (erythroleukemia) and on cells from patients with several kinds of leukemia. There were 14 cases of acute lymphocytic leukemia (ALL), 2 cases of chronic lymp...

journal_title：Journal of clinical laboratory analysis

authors： Marques-Silva VM,De Souza MH,Teixeira MC,Arcuri RA,Rumjanek VM

更新日期：1990-01-01 00:00:00

abstract：:Enteroviruses (EVs) are the most commonly identified cause of aseptic meningitis. Rapid detection and characterization of EV meningitis is essential in making decisions for patient management and treatment. A total of 52 cases of acute aseptic meningitis that occurred from March 2003 to April 2005 were investigated fo...

journal_title：Journal of clinical laboratory analysis

authors： Dumaidi K,Frantzidou F,Papa A,Diza E,Antoniadis A

更新日期：2006-01-01 00:00:00

abstract：:Apoptosis of epithelial hepatocytes plays a pivotal role in acute as well as in chronic liver diseases. The cleavage of cytokeratin-18 (CK-18) by caspases is an early event in the apoptotic process. We therefore sought to investigate serum levels of CK-18 and 20S proteasome in patients with liver cirrhosis, primary gr...

journal_title：Journal of clinical laboratory analysis

authors： Hetz H,Hoetzenecker K,Hacker S,Faybik P,Pollreisz A,Moser B,Roth G,Hoetzenecker W,Lichtenauer M,Klinger M,Krenn CG,Ankersmit HJ

更新日期：2007-01-01 00:00:00

abstract：:Hemoglobin F (HbF) is an effective inhibitor of HbS polymerization. Hydroxyurea (HU) is used to increase HbF synthesis and improve the clinical course of sickle cell disease (SCD) patients. We studied a series of laboratory parameters concerning HbF production and reticulocyte response, and compared data between two g...

journal_title：Journal of clinical laboratory analysis

authors： Borba R,Lima CS,Grotto HZ

更新日期：2003-01-01 00:00:00

abstract：:Previously, an ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) for p24 antigen of HIV-1 was developed. The immune complex comprising 2,4-dinitrophenyl-biotinyl-bovine serum albumin-rabbit anti-p24 Fab' conjugate, p24 antigen, and rabbit anti-p24 Fab' -beta-D-galactosidase conjugate was t...

journal_title：Journal of clinical laboratory analysis

authors： Hashida S,Hashinaka K,Nishikata I,Saito A,Takamizawa A,Shinagawa H,Ishikawa E

更新日期：1996-01-01 00:00:00

abstract：:United States Food and Drug Administration (FDA) in 2010 approved the use of immunosuppressant drug everolimus, which requires therapeutic drug monitoring in whole blood. Taking advantage of structural similarity between sirolimus and everolimus we attempted to rapidly estimate everolimus concentration from apparent s...

journal_title：Journal of clinical laboratory analysis

authors： Dasgupta A,Moreno V,Balark S,Smith A,Sonilal M,Tejpal N,Van Buren CT

更新日期：2011-01-01 00:00:00

abstract：:Circulating bilirubin is thought to function as a physiological antioxidant. One of the decomposition products of this process is the biopyrrins, which include two regioisomers: biotripyrrin-a (1,14,15,17-tetrahydro-2,7,13-trimethyl-1,14-deoxy-3-vinyl-16H-tripyrrin-8,12-dipropionic acid) and biotripyrrin-b (1,14,15,17...

journal_title：Journal of clinical laboratory analysis

authors： Ihara H,Matsumoto T,Morita Y,Hirano A,Okada M,Hashizume N,Shioji I,Yoshimura H

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:This study was performed to compare the clinical efficacies of anterior cruciate ligament (ACL) reconstruction with autologous ligament grafting at different time points. METHODS:Eighty-five patients with ACL were categorized into two groups: Group A (GA, n = 45), who underwent early-stage (≤3 weeks) surger...

journal_title：Journal of clinical laboratory analysis

authors： Hu B,Gao F,Li C,Zhang B,An M,Lu M,Liu Y,Liu Y

更新日期：2020-08-26 00:00:00

abstract：BACKGROUND:Gastric cancer (GC) is one of the global mortality diseases and has a poor prognosis due to the lack of ideal tumor biomarkers. Circular RNAs (circRNAs) are an abundant kind of endogenous RNAs that recently are found play a crucial role in the cancer occurrence and development. Nevertheless, little is known ...

journal_title：Journal of clinical laboratory analysis

authors： Yu X,Ding H,Yang L,Yu Y,Zhou J,Yan Z,Guo J

更新日期：2020-03-01 00:00:00

abstract：:Recombinant HIV-1 p66 (rp66, a subunit of reverse transcriptase (RT), a heterodimer of p66 and p51) was produced in Escherichia coli in three different ways. First, rp66 was produced as a part of the fusion protein of lacZ protein and HIV-1 pol protein consisting of three components: protease (p10), RT (p51/p66), and ...

journal_title：Journal of clinical laboratory analysis

authors： Hashinaka K,Nishikata I,Hashida S,Adachi A,Oka S,Ishikawa E

更新日期：2000-01-01 00:00:00

abstract：BACKGROUND:Definitive detection of hemoglobin (Hb) variants requires DNA sequencing. High-resolution melting (HRM) analysis of polymerase chain reaction (PCR) amplicons was applied to detect and discriminate among uncommon α-Hb variants found in Thailand. METHODS:Uncommon suspected α-Hb variants observed in Hb typing ...

journal_title：Journal of clinical laboratory analysis

authors： Yimniam W,Jindadamrongwech S

更新日期：2016-09-01 00:00:00

abstract：OBJECTIVES:In this paper, a novel, sensitive and rapid method to simultaneously determine the free and total prostate-specific antigen (fPSA and tPSA) in serum by combining a time-resolved fluoroimmunoassay (TRFIA) and immunomagnetic separation was described. METHODS:The new approach uses magnetic particles as an immo...

journal_title：Journal of clinical laboratory analysis

authors： Zhou B,Zhang J,Lv Z,Fan J,Zhang Y,Huang B

更新日期：2017-11-01 00:00:00

abstract：:This study was designed to measure the correlation between left ventricular (LV) remodeling 3 months after successful reperfusion therapy, and the levels of serum N-terminal pro-brain natriuretic peptide (NT-proBNP) and high-sensitivity C-reactive protein (hs-CRP) at 3 days after acute myocardial infarction (AMI), and...

journal_title：Journal of clinical laboratory analysis

authors： Xiaozhou H,Jie Z,Li Z,Liyan C

更新日期：2006-01-01 00:00:00

abstract：BACKGROUND:The aim of this study was to detect reliability of Brucella Coombs gel test (BCGT) by comparing with with ELISA (IgG + IgM), Standard agglutination test, and Brucella immunocapture agglutination methods in serological diagnosis of brucellosis. METHODS:Brucella Coombs gel test (BCGT), Brucella ELISA (IgG + I...

journal_title：Journal of clinical laboratory analysis

authors： Kalem F,Ergün AG,Durmaz S,Doğan M,Ertuğrul Ö,Gündem S

更新日期：2016-09-01 00:00:00

abstract：BACKGROUND:To evaluate clinical features, bacterial characteristics, and risk factors for shock and mortality of immunocompromised patients with Escherichia coli bacteremia. METHODS:A nearly 6-year retrospective study of E coli bacteremia in 188 immunocompromised patients at Xiangya Hospital was conducted. Demographic...

journal_title：Journal of clinical laboratory analysis

authors： Tao X,Wang H,Min C,Yu T,Luo Y,Li J,Hu Y,Yan Q,Liu WE,Zou M

更新日期：2020-08-01 00:00:00

abstract：:A more sensitive and simpler immune complex transfer enzyme immunoassay for antithyroglobulin IgG in serum and its use for the measurement of antithyroglobulin IgG in healthy subjects and patients with thyroid diseases are described. Antithyroglobulin IgG in test serum was reacted simultaneously with dinitrophenylthyr...

journal_title：Journal of clinical laboratory analysis

authors： Kohno T,Mitsukawa T,Matsukura S,Tsunetoshi Y,Ishikawa E

更新日期：1989-01-01 00:00:00

abstract：BACKGROUND:There is a high prevalence of Hepatitis B virus (HBV) infection in China. The aim of this article is to reveal the HBV prevalence and its outcome in southern China by analyzing the HBV serum markers and their relationship to HBV genotype. METHODS:HBV serum markers, HBV-DNA and Alanine aminotransferase (ALT)...

journal_title：Journal of clinical laboratory analysis

authors： Lin S,Liu C,Shang H,Chen H,Yang B,Chen J,Chen Y,Chen D,Ou Q

更新日期：2013-03-01 00:00:00

abstract：BACKGROUND:Neurofilaments are the major cytoskeletal components of neurons, and cell injury leads to their release into the surrounding area. The aim of this study was to compare the cerebrospinal fluid (CSF) and serum (S) concentrations of neurofilament light chains (NFLs) and phosphorylated neurofilament heavy chains...

journal_title：Journal of clinical laboratory analysis

authors： Kušnierová P,Zeman D,Hradílek P,Čábal M,Zapletalová O

更新日期：2019-09-01 00:00:00