Abstract:
:A more sensitive and simpler immune complex transfer enzyme immunoassay for antithyroglobulin IgG in serum and its use for the measurement of antithyroglobulin IgG in healthy subjects and patients with thyroid diseases are described. Antithyroglobulin IgG in test serum was reacted simultaneously with dinitrophenylthyroglobulin and thyroglobulin-beta-D-galactosidase conjugate. The complex formed of antithyroglobulin IgG, dinitrophenyl thyroglobulin, and thyroglobulin-beta-D-galactosidase conjugate was trapped onto two polystyrene balls coated with affinity-purified rabbit (antidinitrophenyl bovine serum albumin) IgG. The polystyrene balls were washed to eliminate nonspecific IgG in the test serum, and the complex was eluted from the polystyrene balls with dinitrophenyl-L-lysine and transferred to two polystyrene balls coated with affinity-purified rabbit (anti-human IgG gamma-chain) IgG. beta-D-Galactosidase activity bound to the polystyrene balls was assayed by fluorimetry using 4-methylumbelliferyl-beta-D-galactoside as substrate. As compared with the previous immune complex transfer enzyme immunoassay, the procedure was simplified by reducing one incubation step and one washing step, and the detection limit of antithyroglobulin IgG in serum (0.1 microgram/liter) was lowered 100-fold. More careful and extensive examination than in a previous study revealed the presence of antithyroglobulin IgG in a large proportion of healthy subjects and in all patients with Graves' disease and chronic thyroiditis.
journal_name
J Clin Lab Analjournal_title
Journal of clinical laboratory analysisauthors
Kohno T,Mitsukawa T,Matsukura S,Tsunetoshi Y,Ishikawa Edoi
10.1002/jcla.1860030306subject
Has Abstractpub_date
1989-01-01 00:00:00pages
163-8issue
3eissn
0887-8013issn
1098-2825journal_volume
3pub_type
杂志文章abstract:BACKGROUND:Automated flow cytometry-based urine analyzer is increasingly being used to identify and enumerate cells and particles in urine specimens. It measures electrical conductivity which could be transformed to osmolality. Using this machine, all urine specimens could be screened for osmolality without requiring a...
journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.23586
更新日期:2020-09-24 00:00:00
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journal_title:Journal of clinical laboratory analysis
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journal_title:Journal of clinical laboratory analysis
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journal_title:Journal of clinical laboratory analysis
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journal_title:Journal of clinical laboratory analysis
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.21914
更新日期:2016-09-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
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更新日期:2013-07-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.23510
更新日期:2020-08-26 00:00:00
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.20053
更新日期:2005-01-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.23306
更新日期:2020-08-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
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更新日期:1990-01-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.20470
更新日期:2011-01-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.23434
更新日期:2020-10-01 00:00:00
abstract:INTRODUCTION:The pro- and anti-inflammatory cytokines play crucial role in the development and functions of placenta. Any changes in these cytokines may be associated with many pregnancy-related disorders like preeclampsia. Therefore, the present study is aimed to study the expression of pro-inflammatory (TNF-α, IL-6) ...
journal_title:Journal of clinical laboratory analysis
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doi:10.1002/jcla.22834
更新日期:2019-05-01 00:00:00
abstract:BACKGROUND:Gastric carcinoma (GC) is a familiar carcinoma and serious threat to human health. We investigated the efficacy and mechanism of circular RNA hsa_circ_0001649 on the growth, migration, and invasion of GC cells. METHODS:microRNA (miR)-20a and hsa_circ_0001649 expression was investigated by RT-qPCR and was ch...
journal_title:Journal of clinical laboratory analysis
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doi:10.1002/jcla.23235
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journal_title:Journal of clinical laboratory analysis
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journal_title:Journal of clinical laboratory analysis
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doi:10.1002/(sici)1098-2825(1998)12:1<54::aid-jcla9>3.
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章,评审
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journal_title:Journal of clinical laboratory analysis
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更新日期:1992-01-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
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更新日期:2000-01-01 00:00:00
abstract:AIM:To investigate the presence of antipituitary antibodies (APA) in the serum of patients undergoing gastroenteropathies (GEP) other than celiac disease (CD). METHODS:APA were analyzed in GEP patients (n = 103), CD patients (n = 94), idiopathic growth hormone (GH) deficiency patients (n = 21), and 98 age- and sex-mat...
journal_title:Journal of clinical laboratory analysis
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更新日期:2014-01-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.20418
更新日期:2010-01-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
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更新日期:2009-01-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
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更新日期:2016-11-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
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更新日期:2020-08-30 00:00:00
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.20245
更新日期:2008-01-01 00:00:00
abstract::Quantitative determination of albumin (ALB) in human urine is important to assess kidney functions in a variety of diseases. Recently, Siemens released an improved Microalbumin assay (microALB_2) to measure urinary ALB on the automated, random access ADVIA 1650/1800, ADVIA 2400, and ADVIA 1200 Chemistry Systems. We ev...
journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
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更新日期:2009-01-01 00:00:00