Yeast-secreted bovine herpesvirus type 1 glycoprotein D has authentic conformational structure and immunogenicity.

Abstract:

:Bovine herpesvirus-1 (BHV-1) glycoprotein D (gD), an envelope glycoprotein, engenders mucosal and systemic immunity protecting cattle from viral infection. Production of gD with authentic immunogenicity is required for a subunit vaccine. We placed the truncated BHV-1 gD gene, lacking its putative transmembrane and cytoplasmic domains, under the control of the methanol-inducible AOX1 promoter in the yeast Pichia pastoris. Truncated BHV-1 gD (tgD) was efficiently secreted into the culture medium as a 68 kDa protein using either the yeast alpha prepro or native BHV-1 gD signal sequences. The yeast-secreted tgD had N-linked glycosylation and appears to have authentic conformational structure and immunogenicity based on the following observations A panel of monoclonal antibodies recognizing five neutralizing epitopes reacted with yeast tgD. Sera from yeast tgD-immunized mice immunoprecipitated native BHV-1 gD and neutralized BHV-1 infection in vitro. Yeast tgD competitively blocked all reaction between native gD and monospecific gD polyclonal sera from cattle. Based on these data, yeast-derived BHV-1 tgD is an excellent candidate for a subunit vaccine.

journal_name

Vaccine

journal_title

Vaccine

authors

Zhu X,Wu S,Letchworth GJ 3rd

doi

10.1016/s0264-410x(96)00234-4

subject

Has Abstract

pub_date

1997-04-01 00:00:00

pages

679-88

issue

6-7

eissn

0264-410X

issn

1873-2518

pii

S0264-410X(96)00234-4

journal_volume

15

pub_type

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