Abstract:
:Pectin methyltransferase (PMT) catalyzing the transfer of the methyl group from S-adenosyl-L-methionine (SAM) to the C-6 carboxyl group of galactosyluronic acid residues in pectin was found in a membrane preparation of etiolated hypocotyls from 6-d-old soybean (Glycine max Merr.). The enzyme was maximally active at pH 6.8 and 35-40 degrees C, and required 0.5% (w/v) Triton X-100. The incorporation of the methyl group was significantly enhanced by addition of a pectin with a low (22%) degree of methyl-esterification (DE) as exogenous acceptor substrate. The apparent Michaelis constants for SAM and the pectin (DE22) were 0.23 mM and 66 microg x ml(-1), respectively. Attachment of the methyl group to the carboxyl group of the pectin via ester linkage was confirmed by analyzing radiolabeled product from incubation of the enzyme with [14C]methyl SAM and the acceptor pectin. Size-exclusion chromatography showed that both enzymatic hydrolysis with a pectin methylesterase and a mild alkali treatment (saponification) led to the release of radioactive methanol from the product. Enzymatic hydrolysis of the product with an endopolygalacturonase degraded it into small pectic fragments with low relative molecular mass, which also supports the idea that the methyl group is incorporated into the pectin. The soybean hypocotyls were fractionated into their cell wall components by successive extraction with water, EDTA, and alkali treatment. Among the resulting polysaccharide fractions, high PMT activity was observed when a de-esterified polysaccharide derived from the EDTA-soluble fraction (the pectic fraction) was added as an alternative acceptor substrate, indicating that the enzyme may be responsible for producing methyl-esterified pectin in vivo.
journal_name
Plantajournal_title
Plantaauthors
Ishikawa M,Kuroyama H,Takeuchi Y,Tsumuraya Ydoi
10.1007/s004250050680subject
Has Abstractpub_date
2000-04-01 00:00:00pages
782-91issue
5eissn
0032-0935issn
1432-2048pii
10.1007/s004250050680journal_volume
210pub_type
杂志文章相关文献
PLANTA文献大全abstract::The properties of the water-soluble metabolites of [(3)H]gibberellin A1 ([(3)H]GA1) from lettuce (Lactuca sativa L.) hypocotyls were compared with those of authentic samples of gibberellin (GA) glucosyl esters and ethers. Partitioning against l-butanol at high and low pH was not an efficient method of differentiating ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385994
更新日期:1977-01-01 00:00:00
abstract::Taking into account the literature on the so-called sieve-tube slime ("mictoplasm", "slime strands") and regarding its fine structure more in detail the term plasmatic filament ("Plasmafilament") is proposed and will be used in this paper to characterize the individual exceedingly fine subunit of the plasmatic network...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385169
更新日期:1967-03-01 00:00:00
abstract:MAIN CONCLUSION:Exogenous SA treatment at appropriate concentrations promotes adventitious root formation in cucumber hypocotyls, via competitive inhibiting the IAA-Asp synthetase activity of CsGH3.5, and increasing the local free IAA level. Adventitious root formation is critical for the cutting propagation of horticu...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-020-03467-2
更新日期:2020-10-07 00:00:00
abstract::In the genome of Nostoc punctiforme PCC 73102, three functional β-carotene ketolase genes exist, one of the crtO and two of the crtW type. They were all expressed and their corresponding enzymes were functional inserting 4-keto groups into β-carotene as shown by functional pathway complementation in Escherichia coli. ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-013-1846-8
更新日期:2013-05-01 00:00:00
abstract::The genomic sequence of a rice (Oryza sativa L.) glycine-rich protein (GRP) gene, designated Osgrp-2, has been previously determined (GenBank U40708). Primer extension analysis indicated that transcription starts 47 bp upstream of the translation start codon. To gain an insight into the transcriptional regulation of t...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-002-0934-y
更新日期:2003-03-01 00:00:00
abstract::The basic helix-loop-helix (bHLH) transcription factors (TFs) comprise one of the largest families of TFs involved in developmental and physiological processes in plants. Here, we describe the functional characterization of two bHLH TFs (NtAn1a and NtAn1b) isolated from tobacco (Nicotiana tabacum) flowers. NtAn1a and ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-011-1407-y
更新日期:2011-08-01 00:00:00
abstract::We have investigated the regulation of sucrose storage in cell-suspension cultures of sugarcane. When grown in batch culture, sucrose accumulation commences after about 5 d, when the nitrogen supply is exhausted. Sucrose storage is also induced by decreasing the nitrogen supply to cells growing in a chemostat. The mea...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00197564
更新日期:1991-12-01 00:00:00
abstract::The differential growth causing second positive phototropic curvature in intact, black-capped and decapitated Avena coleoptiles has been measured. In all cases the curvature is brought about by a cessation in growth of the illuminated side. The fact that shading the apex does not significantly alter the initial steps ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00429452
更新日期:1982-08-01 00:00:00
abstract::Air-grown cells of Porphyridium purpurem contain appreciable carbonic-anhydrase activity, comparable to that in air-grown Chlamydomonas reinhardtii, but activity is repressed in CO2-grown cells. Assay of carbonic-anhydrase activity in intact cells and cell extracts shows all activity to be intracellular in Porphyridiu...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00393867
更新日期:1987-12-01 00:00:00
abstract::The sites of transcription and translation of carotenoid pigments were studied in synchronously grown cells of Chlamydomonas reinhardtii Dang. Rifampicin, cycloheximide and spectinomycin were used to distinguish between the nuclear-cytoplasmic genetic system and the genetic system of the chloroplast. Since rifampicin ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00386737
更新日期:1973-03-01 00:00:00
abstract:MAIN CONCLUSION:In this genome-wide association study, we obtained novel insights into the genetic basis of the effect of herbivory or drought stress on the level of resistance against the fungus Botrytis cinerea. In nature, plants function in complex environments where they encounter different biotic and abiotic stres...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-018-3065-9
更新日期:2019-04-01 00:00:00
abstract::Sesuvium portulacastrum is a common halophyte growing well in adverse surroundings and is exploited mainly for the environmental protection including phytoremediation, desalination and stabilization of contaminated soil. In the present investigation, attempts have been made on the decolorization of a toxic textile dye...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-011-1556-z
更新日期:2012-05-01 00:00:00
abstract::Hemoglobin gene expression is upregulated during hypoxia. To determine whether the induction occurs via similar mechanisms that have been proposed for other hypoxically induced proteins, barley (Hordeum vulgare L.) aleurone layers were treated with various agents that interfere with known components of signal transduc...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-005-0094-y
更新日期:2006-02-01 00:00:00
abstract:MAIN CONCLUSION:A chemical screen of plant-derived compounds identified holaphyllamine, a steroid, able to trigger defense responses in Arabidopsis thaliana and improve resistance against the pathogenic bacterium Pseudomonas syringae pv tomato DC3000. A chemical screen of 1600 plant-derived compounds was conducted and ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-017-2755-z
更新日期:2017-12-01 00:00:00
abstract:MAIN CONCLUSION:AtNAP , an Arabidopsis NAC transcription factor family gene, functions as a negative regulator via transcriptional repression of AREB1 in salt stress response. AtNAP is an NAC family transcription factor in Arabidopsis and is known to be a positive regulator of senescence. However, its exact function an...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-016-2609-0
更新日期:2017-02-01 00:00:00
abstract::Supernatant and resuspended pellet samples from a centrifugation of homogenised, etiolated oat seedlings were prepared and assayed spectrophotometrically for phytochrome in the presence and absence of added calcium carbonate (CaCO3) particles under a variety of conditions. At a constant sample thickness, in the absenc...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387903
更新日期:1982-03-01 00:00:00
abstract::Freeze-etch observations of protoplasts isolated from tobacco (Nicotiana tabacum L.) mesophyll tissue and tomato (Lycopersicum esculentum Mill.) fruit locule tissue are described which clarify earlier observations (Burgess, J., Fleming, E.N., Planta 131, 173-178, 1976; Planta 133, 267-273, 1977), obtained using scanni...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00389379
更新日期:1978-01-01 00:00:00
abstract::Uncovered in studies on photosynthesis 35 years ago, redox regulation has been extended to all types of living cells. We understand a great deal about the occurrence, function, and mechanism of action of this mode of regulation, but we know little about its origin and its evolution. To help fill this gap, we have take...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-012-1803-y
更新日期:2013-02-01 00:00:00
abstract::Crude extracts of dark-kept resting cells of a chlorophyll-free, carotenoid-containing mutant of Chlorella vulgaris Beijerinck (211-11h/20) were found to convert 14.44±0.77 nmol PEP per min and mg protein into pyruvate by the action of pyruvate kinase (=PK; EC 2.7.1.40). When such cells were exposed to blue light (λ<5...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00397172
更新日期:1976-01-01 00:00:00
abstract::The surface of plants is covered by cuticular wax, which contains a mixture of very long-chain fatty acid (VLCFA) derivatives. This wax surface provides a hydrophobic barrier which reduces non-stomatal water loss. One component of the cuticular wax is the alkyl esters, which typically contain a VLCFA esterified to an ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-007-0489-z
更新日期:2007-07-01 00:00:00
abstract::Vacuoles isolated from red beet (Beta vulgaris L.) storage roots contain Na(+) and K(+) but their analysis does not give reliable information about the size of vacuolar pools of these ions in vivo. Analyses of isolated vacuoles indicated that between 53% and 90% of the Na(+) was located in the vacuole and that the vac...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392083
更新日期:1983-11-01 00:00:00
abstract::The rbcS1, 2 and 3 genes of Phaseolus vulgaris are identical in coding sequence and we have studied their expression using gene-specific probes derived from their 3' non-coding regions. The genes differ in their relative levels of expression but show only minor qualitative differences in their regulation. Transcripts ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00197583
更新日期:1996-01-01 00:00:00
abstract::Developing wheat (Triticum aestivum L.) endosperm was examined using ultrathin sections prepared from tissues harvested at 5, 9, 16 and 25 d after flowering. Protein bodies were evident by 9 d and displayed a variety of membranous structures and inclusions. The Golgi apparatus was a prominent organelle at all stages, ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392442
更新日期:1988-11-01 00:00:00
abstract::Narbonin is a 2S protein from the globulin fraction of narbon bean (Vicia narbonensis L.) cotyledons. Its amino acid composition and the pattern of its regulated accumulation in developing seeds led to the suggestion that narbonin could be a storage protein. Therefore, it was expected to be present in protein bodies o...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00050163
更新日期:1997-09-01 00:00:00
abstract::The cell-wall formation in the egg of Pelvetia fastigiata (J.G. Agardh) DeToni (Fucaceae) was studied with freeze-fracture. 1. The wall is lamellated with microfibrils approximately parallel in each lamella. The average orientation of microfibrils turns about 35° in each subsequent lamella. This slow turn gives rise t...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00386007
更新日期:1976-01-01 00:00:00
abstract::Mitochondria isolated from greening etiolated laminae of Avena sativa L. show changes in the permeability of their inner membranes during chloroplast development similar to those described earlier for plastids. Oxalo-acetate, succinate and α-keto-glutarate permeate most readily inner membranes of mitochondria isolated...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387340
更新日期:1976-01-01 00:00:00
abstract::Sugar-synthesis and -transport patterns were analyzed in Coleus blumei Benth. leaves to determine where galactinol, raffinose, and stachyose are made and whether phloem loading includes an apoplastic (extracellular) step or occurs entirely within the symplast (plasmodesmata-connected cytoplasm). To clarify the sequenc...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00195663
更新日期:1992-06-01 00:00:00
abstract::Embryogenesis occurs in cells from brome grass grown in suspension culture in defined medium. Exogenous hormones are not required. The embryos develop into plants which lack chlorophyll. ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00384708
更新日期:1970-12-01 00:00:00
abstract::We studied changes induced by cold on electron transfer pathways (linked to NADH or NADPH oxidation) in endoplasmic reticulum of rapeseed hypocotyls (Brassica napus L.) from a freezing-sensitive variety (ISL) and freezing-tolerant variety (Tradition). Plantlets were grown at 22 degrees C then submitted to a cold shock...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-008-0793-2
更新日期:2008-10-01 00:00:00
abstract::Haploid plants of Nicotiana tabacum L. were produced in greater numbers from anthers on agar medium containing activated charcoal than on the same medium without charcoal. ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00390524
更新日期:1974-09-01 00:00:00