Abstract：
BACKGROUND:Because treatment of advanced pancreatic cancer is often unsuccessful, early detection is important. Codon 12 c-Ki-ras mutations have been found in 80-90% of pancreatic cancer cases and are a potential early marker for pancreatic cancer, but obtaining tissue or fluid for analysis can be difficult. We therefore evaluated whether mutant c-Ki-ras could be detected in bile samples obtained from pancreatic cancer patients. METHODS:DNA was isolated from bile specimens obtained from 20 patients with pancreatic cancer. The mutant-enriched PCR technique (ME-PCR) was used to amplify and detect point mutations at codon 12 of the c-Ki-ras oncogene. RESULTS:In 17 cases sufficient DNA for amplification was obtained; 14 had mutant c-Ki-ras alleles. Cytological evaluation of the bile was performed in 11 of these cases, but was positive in only two cases; both were positive for codon 12 c-Ki-ras mutations. Of the 9 cytologically negative biliary specimens, ME-PCR was positive in six. CONCLUSIONS:Codon 12 c-Ki-ras mutations can be successfully identified in PCR-amplified DNA from bile samples obtained from patients with advanced pancreatic cancer. This technique may supplement cytologic techniques for diagnosing pancreatic cancer and may be capable of identifying individuals at risk for this disease.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Abbruzzese JL,Evans DB,Raijman I,Larry L,King T,Leach SD,Frazier MLsubject
Has Abstractpub_date
1997-03-01 00:00:00pages
795-801issue
2Aeissn
0250-7005issn
1791-7530journal_volume
17pub_type
杂志文章abstract：BACKGROUND/AIM:4-methylumbelliferone (4-MU) has received considerable attention due to its potential for cancer treatment since it inhibits cell proliferation, migration and invasion. An increasing body of evidence suggests that extracellular matrix (e.g. hyaluronan) and nutrients (e.g. glucose) in the tumor microenvir...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-09-01 00:00:00
abstract：:Interleukin-10 (IL-10) is an immunosuppressive cytokine involved in carcinogenesis via immune escape. The present study aimed at evaluating the contribution of IL-10 promoter A-1082G (rs1800896), T-819C (rs3021097), A-592C (rs1800872) genetic polymorphisms to the risk of lung cancer in Taiwan. Associations of three IL...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-12-01 00:00:00
abstract：BACKGROUND:Prognosis of renal cell carcinoma (RCC) differs within the same stage and grade. Our aim was to investigate the incidence of COX-2 in primary RCC tumors at different stages according to the occurrence of metastasis, and the impact of this biomarker on the survival of RCC patients. PATIENTS AND METHODS:The c...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-07-01 00:00:00
abstract：BACKGROUND:Extracts from Viscum album L. (VAL), which are one of the most widely used alternative cancer therapies in Europe, have been recognised to induce apoptosis. A clinically relevant direct anti-tumour effect might be induced only by their intratumoural or intrapleural injection, and thus effectiveness is highly...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：BACKGROUND:Basic conventional prognostic factors for breast cancer include the age of the patient, tumor grade, regional lymph nodes status, and estrogen (ER) and progesterone (PR) receptor status. Positivity of the HER2 receptor (c-erbB-2) seems to be a new prognostic and predictive factor. Prognostic factors seem to ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2007-07-01 00:00:00
abstract：:We report a case of secondary chondrosarcoma arising in the ilium showing spontaneous regression after frequent local tumor recurrences followed by multiple surgeries of marginal or intralesional excision. The patient was a 16-year-old boy who had been diagnosed as having multiple exostosis from 9 years of age. He exp...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-09-01 00:00:00
abstract：:A new cell line, designated CC2/CUHK2, was established from a squamous carcinoma of the uterine cervix from a Chinese patient. The cell line grew well without interruption for over 12 months and over 90 passages. The doubling time of CC2/CUHK2 was 72 hours at passage 57. When seeded at a density of 1.5 x 10(3) cells p...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1993-07-01 00:00:00
abstract：BACKGROUND:High thymidine kinase (TK) activity in cancer cells could counteract adjuvant chemotherapy directed at the inhibition of de novo DNA synthesis. TK is an independent prognostic factor in breast cancer patients receiving adjuvant chemotherapy. MATERIAL AND METHODS:In this paper, we describe the effects of ext...
journal_title：Anticancer research
pub_type： 杂志文章,多中心研究
doi：
更新日期：2000-03-01 00:00:00
abstract：:Gross cystic disease (GCD) represents an advanced form of fibrocystic disease of the breast. Bearers of macrocysts have been reported to be at risk of developing breast cancer. Natural killer (NK) cells are a lymphocyte subset deeply involved in immunosurveillance against neoplasia. We investigated whether breast cyst...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1994-03-01 00:00:00
abstract：:A rat rhabdomyosarcoma clonal line RMS/8 was cotransfected with genomic DNA of procaryotic or eucaryotic origin and neomycin resistance plasmid pKOneo. The lung colonizing and metastatic capacity of transfected cells was evaluated. When col- meta- RMS/8 cells were transfected, an increase in lung colonizing but not in...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1987-03-01 00:00:00
abstract：:T-CAR1 is a human carcinoma cell line established from a brain metastasis. The tumour cells overexpress EGFR and contain an amplified EGFR gene. In vitro in the presence of 5% human serum the tumour cells grow as adherent cells in monolayer. Shortly after exposure to EGF a large number of tumour cells round up and det...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1995-05-01 00:00:00
abstract：:The effects of an anti-CD3 mAb on induction of non-MHC restricted cytolysis was investigated. Peripheral blood mononuclear cells (PBMC) from normal donors (29) and cancer patients (18) were cultured in 100 U/ml of interleukin-2 (rIL-2) with and without anti-CD3 mAb (OKT3, 10 ng/ml) for the first 48 hours of incubation...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1992-11-01 00:00:00
abstract：AIMS AND BACKGROUND:Radiation therapy holds a fundamental role in oncological emergencies such as superior vena cava syndrome, spinal cord compression and endocranial hypertension. The purpose of our study was, by comparing schedules of treatment, to confirm the efficacy of hypofractionated radiation therapy. METHODS:...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：2001-05-01 00:00:00
abstract：BACKGROUND:Kita-Kyushu lung cancer antigen-1 (KK-LC-1) is a cancer/testis antigen and predominant target for cancer immunotherapy. Its detection is only established based on gene expression. In this study, we established a monoclonal antibody against KK-LC-1 to detect its protein expression in formalin-fixed samples. ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12937
更新日期：2018-10-01 00:00:00
abstract：BACKGROUND/AIM:Many studies have shown an antiproliferative, anti-inflammatory, anti-angiogenetic, and apoptosis-inducing effect of Vitamin D. A vitamin D deficiency has been associated with an increased risk for different types of cancer. This study examined vitamin D 25(OH)D levels in gynaecological cancers in compar...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13983
更新日期：2020-01-01 00:00:00
abstract：BACKGROUND:Our purpose was to determine the effects of amifostine, a cytoprotective agent, on doxorubicin tolerance and cardiotoxicity in rats. MATERIALS AND METHODS:Male Wistar rats were treated every other day with an intraperitoneal injection of amifostine or saline 30 minutes before intraperitoneal injection of do...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：:The multifaceted involvement of the active vitamin D metabolite 1,25-dihydroxyvitamin D3 (henceforth referred to by the synonyms 1,25(OH)2D3, calcitriol or vitamin D) in blunting the growth of cancer cells is amply recognized. In this review we focused our attention on the cross-talk between 1,25 (OH)2D3 and the tumor...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：10.21873/anticanres.11216
更新日期：2016-12-01 00:00:00
abstract：:We have recently identified ICBP90 as being a protein able to bind in vitro a CCAAT box of the topoisomerase II alpha gene promoter. The aim of the present work was to check whether ICBP90 is able to regulate in vivo topoisomerase II alpha expression in human lung fibroblasts under various proliferating conditions. Tr...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2002-11-01 00:00:00
abstract：:Metals and metal compounds have been used in medicine for several thousands of years. In this review we summarized the anti-cancer activities of the ten most active metals: arsenic, antimony, bismuth, gold, vanadium, iron, rhodium, titanium, gallium and platinum. The first reviewed metal, arsenic, presents the anomaly...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2004-05-01 00:00:00
abstract：:In this immunohistochemical study we investigated the expression of Bcl-2 and Bax apoptosis related proteins in node-negative breast carcinomas. The results were correlated with the recurrence rate of the patients. In order to avoid the influence of the most important tumor prognostic parameters we selected two groups...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-07-01 00:00:00
abstract：:Homogeneous subpopulations, which are endowed with low or high metastatic potential, were selected from Lewis lung carcinoma (3LL) in an attempt to correlate metastatic phenotype with specific properties of tumor cells. Since the growth of malignant cells at secondary sites could depend on their ability to respond to ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1990-11-01 00:00:00
abstract：:It was the aim of this study to compare drug-resistant sublines of the murine P388 in relation to resistance markers, the resistant phenotype and immunogenicity. Resistance to drugs either belonging to the MDR type (Doxorubicin, Vincristine, Mitoxantrone) or to the non-MDR type (Methotrexate) was generated in vivo in ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1994-09-01 00:00:00
abstract：:Hepatocellular carcinoma is one of the most common malignancies in the world, with the liver being the second most frequently involved organ in metastatic disease. Although the gold standard treatment for malignant liver disease is surgical resection, only few patients can undergo such an intervention. This explains t...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2007-07-01 00:00:00
abstract：:Using a cultured human colon cancer cell line (Col 2), a structurally diverse group of chemopreventive agents was evaluated for their potential to induce apoptosis. As a result, 1,4-phenylenebis(methylene)selenocyanate (p-xylylselenocyanate; p-XSC) was found to be active in this process. p-XSC, a synthetic organoselen...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：BACKGROUND:The aim of this study was to investigate the diagnostic value of FDG PET in the follow-up for breast cancer in disease-free patients and patients suspected of having recurrent or metastatic disease. As a single imaging tool, PET can be compared with the conventional diagnostic means used for different examin...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：2003-03-01 00:00:00
abstract：:The electrophysiological effects of gallium chloride (Ga ) and its activity on arrhythmias induced by digitalis were investigated in guinea pig papillary muscle. KCl microelectrodes were used to record transmembrane electrical activity from Purkinje cells from the papillary muscle of guinea pig hearts during superfusi...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:Smokeless tobacco consumption is causally associated with oral cavity cancers; however, extensive cytogenetic studies have not been done. In the present study, individuals consuming dry snuff or tobacco with lime have been studied for frequency of micronucleated cells (MNC) in exfoliated buccal mucosa and chromosome a...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1993-11-01 00:00:00
abstract：:In order to identify genes associated with metastasis of mammary carcinoma, we compared the transcriptional profile (Affymetrix chip technology) of two cell lines derived from primary mammary carcinoma, three cell lines derived from bone marrow micrometastasis, a cell line derived from a lymph node metastasis as well ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
abstract：BACKGROUND/AIM:The impact of testosterone or obesity on the pathological grade of prostate cancer remains controversial. Therefore, in this study, we investigated the relationship of serum testosterone and body mass index (BMI) to Gleason score at biopsy. PATIENTS AND METHODS:This study included 128 Japanese patients ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-11-01 00:00:00
abstract：:The experimental techniques, methods of evaluation, and environmental implications of the findings of chemical carcinogenesis studies and related subject areas are herein critically reviewed. It is concluded that firstly, certain presently used procedures provide the best possibilities for the study of the hazardous n...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1985-09-01 00:00:00
