[Influence of visible interferences on biochemical assays realised on Au 5231, AU 5223 (Olympus) and CL 7200 (Shimadzu)].

Abstract:

:The impact of the major interferents (hemolysis, bilirubin, turbidity), on the quality of biochemical tests, was evaluated on multiparametric analysers (CL 7200 Shimadzu, Japan/Ciba-Corning, France; AU 5231 and AU 5223 Olympus, Japan/bioMérieux, France), according to the SFBC instructions. Interferences were detected in 33 cases upon 165 tests realized, that is to say 20% of the performed analysis. Turbidity was the most frequent cause of interference (7.8%), followed by hemolysis (8.5%) and bilirubin (3.6%). The use of a sample blank, a bireagent, the change of reagent, the change of the secondary wavelength or the modification of the measurement times, allowed us to reduce more than 80% of the interferences. Only three interferences remained: hemolysis upon the measurement of TGO and potassium, and bilirubin upon the measurement of creatinine. For these parameters, a suitable note using the Olympus factors (semi quantitative expression of the importance of the three interferents) is reported on the answer sheet.

journal_name

Ann Biol Clin (Paris)

authors

Frémont S,Combe AM,Mecrin M,Tronel H,Galland AV,Scholl C,Nicolas JP

subject

Has Abstract

pub_date

1996-01-01 00:00:00

pages

309-20

issue

8-9

eissn

0003-3898

issn

1950-6112

journal_volume

54

pub_type

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